Objective:To investigate the effect of Dan Sen on gastric mucosal Na +-K +-ATPase activity and gastric transmucosal potential difference in rats during severe intraperitoneal infection.Methods:The intraperitoneally infected rat model was established by cecal ligation and puncture(CLP).Assay of Na +-K +-ATPase activity in gastric mucosal tissue was conducted by biochemistry method.The electric-physiological recorder meter was used to measure gastric mucosal potential difference.Results:The activity of Na +-K +-ATPase markedly decreased in infected group at 3h after perforation,compared with the control group ( P

ObjectiveTo analyze the characteristics of severe trauma patients with acute kidney injury (AKI) receiving renal replacement therapy (RRT), in order to look for the risk factors of AKI and the opportune time for the initiation of RRT on prognosis.Methods A retrospective cohort study involving consecutive patients with severe trauma in emergency intensive care unit (ICU) in the Second Affiliated Hospital of Zhejiang University School of Medicine, from August 2011 to December 2014, was conducted. Inclusion criteria included age≥18 years, injury severity score (ISS)> 16, AKI receiving RRT, and the duration of hospital stay> 24 hours. The general data, the risk factors of AKI, the prognostic indicators, and the information of RRT were recorded. All patients were divided into two groups according to the prognosis, the time of onset of AKI and the initiation time of RRT. The independent risk factors for prognosis were screened by binary logistic regression analysis.Results Seventy-three patients were eligible for enrollment, including 48 deaths (65.8%); 49 patients suffered from AKI≤48 hours after trauma (early stage group), and in 24 patients it was longer than 48 hours (late stage group). In 55 patients RRT was routinely started (routine RRT group), 18 patients underwent RRT ahead of routine criteria decided by the judgment of the attending doctor (earlier RRT group). The main risk factors of RRT in traumatic patients with AKI were shock and sepsis, each accounted for 90.4% and 53.4%. Compared with survival group, in death group, the proportion of male patients was lower (70.8% vs. 100.0%,χ2 = 7.238,P = 0.007), acute physiology and chronic health evaluationⅡ (APACHEⅡ) scores were higher (23.7±5.1 vs. 14.4±3.7,t = 8.031,P< 0.001), Glasgow coma score (GCS) was lower [5.0 (3.0, 15.0) vs. 15.0 (8.0, 15.0),U = 320.000,P = 0.001], incidence of shock and sepsis was higher (97.9% vs. 76.0%,χ2 =6.755,P = 0.009; 64.6% vs. 32.0%,χ2 = 7.014,P = 0.008), the rate of use of contrast medium was lower (27.1% vs. 56.0%,χ2 = 5.898,P = 0.015), the time for the diagnosis of AKI post trauma was delayed [days: 2 (1, 5) vs. 2 (1, 2), U = 762.000,P = 0.049], the time for the initiation of RRT post trauma was later [days: 6.0 (3.0, 12.0) vs. 3.0 (2.0, 4.5), U = 868.500,P = 0.002], the recovery rate of renal function at discharge was lower (10.4% vs. 100.0%,χ2 = 54.497, P< 0.001). Compared with late stage group, in early stage group, the mortality was lower (55.1% vs. 87.5%,χ2 =7.509,P = 0.006), and the incidence of sepsis before AKI was also lower (38.8% vs. 83.3%,χ2 = 12.854,P< 0.001). Compared with routine RRT group, the recovery of renal function at discharge was better with a lower mortality rate in the earlier RRT group, but the difference was considered to be insignificant (55.6% vs. 36.4%,χ2 = 2.064,P = 0.151;50.0% vs. 70.9%,χ2 = 2.633,P = 0.105). Logistic regression analysis showed GCS [odds ratio (OR) = 0.852, 95%confidence interval (95%CI) = 0.747-0.972,P = 0.017], shock before AKI (OR = 85.350, 95%CI = 5.682-1 282.073, P = 0.001), and sepsis before AKI (OR = 11.499, 95%CI = 2.127 - 62.161,P = 0.005) were independent risk factors for the judgment of prognosis.Conclusions Shock and sepsis are the major risk factors of RRT in trauma patients with AKI. Shock, sepsis and traumatic brain injury are the independent risk factors of death. Perhaps early initiation of routine RRT cannot improve the outcome of the patients with posttraumatic renal insuficiency.

Asialoglycoprotein receptor(ASGPR) is a specific receptor of mammalian hepatocytes.Reduction in ASGPR concentration has been proven to appear in liver cirrhosis and liver cancer.By liver ASGPR scintigraphy with 99mTcGSA,some indexes such as HH15、LHL15、[R]_0、R_0 can be obtained,which is useful for the evaluation of liver function.When combining with the techniques of functional scintigraphy and single photon emission computed tomography(SPECT),it becomes possible to functionally simulate the extension of hepatic resection and predict to some extent postoperative outcomes related to liver function.It is still a new professional field internationally,and almost absent domestically.It could be an important tool for quantificational evaluation of the risk of liver surgery and help determining the surgical procedures.We have here,with some of our research experiences in this field,written a review of ASGPR scintigraphy.

Whether the hepatic pipestem fibrosis induced by schistosomiasis japonica can result in cirrhosis,confusion exists among parasitologists in China.Evidence from national and international pathologists and clinicians confirmed that the pipestem fibrosis could develop into cirrhosis undoubtedly.Owing to different pathogenic causes,the characters of cirrhosis are different.To re-understand cirrhosis induced by schistosomiasis japonica is of significance for the diagnosis and treatment of the advanced patient.

Objective To explore the effect of Buzhong Yiqi combined with low frequency impact feedback and rehabilitation training on postpartum pelvic floor rehabilitation.Methods100 cases of maternal fertility were randomly divided into observation group and control group, and 50 cases were randomly divided into observation group and control group.The control group was given low-frequency shock and electrical stimulation and rehabilitation training intervention, the observation group in the control group at the same time plus the addition of Buzhong Yiqi Tang treatment, were treated for 3 months.To evaluate and compare the pelvic floor muscle function, vaginal tension, vaginal pressure and nocturia before and after treatment, and compare the quality of life and the occurrence of complications after treatment.ResultsThere were no significant differences in the parameters of the pelvic floor muscle, vaginal tension, vaginal pressure and nocturia before treatment.and the maximal muscle voltage of the pelvic floor muscle and the pelvic floor muscle contraction were 60 s, the mean muscle voltage values of the pelvic floor muscle were significantly increased (P<0.05).However, the vaginal muscle tension and vaginal pressure in the observation group were significantly higher than those in the control group (P<0.05), and the vaginal muscle tension and vaginal pressure were significantly higher (P<0.05), but the number of nocturia in the observation group was significantly lower than that in the control group (P<0.05), and the FS-FI score of the observation group was significantly higher than that of the control group (P<0.05).The incidence of urinary incontinence was 4.26% and 6.38% in the observation group were significantly lower than those in the control group (16.67%, 18.75%, P<0.05).ConclusionBuzhong Yiqi Fang combined with low-frequency shock and electric stimulation, rehabilitation training treatment is conducive to promoting maternal postpartum pelvic floor rehabilitation, improve sexual quality of life, and can effectively reduce the incidence of pelvic organ prolapse, urinary incontinence.

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Objective To explore the efficacy of internal fixation in the treatment of intra-articular calcaneum fractures via the sinus tarsi approach . Methods A retrospective study was made on 55 feet with intra-articular calcaneum fractures in 51 patients treated with open reduction and intemal fixation via sinus tarsi approach from January 2010 to June 2015.According to the Sanders classification, there were 15 feet of type Ⅱfractures and 40 feet of type Ⅲfractures. Results All the patients were followed up for a mean period of 15.7 months (range, 6-33 months).The fractures were completely healed .According to the Maryland Foot Scores , the operative effect was excellent in 43 feet, good in 7 feet, fair in 4 feet, and poor in 1 foot.The excellent and good rate was 90.9%(50/55).The postoperative width of the calcaneum , B?hler angle, and Gissane angle were significantly improved than before operation (P=0.000). Conclusion Open reduction and internal fixation via sinus tarsi approach is a simple and effective method for minimally invasive treatment of Sanders type Ⅱand Ⅲintra-articular calcaneum fractures .

Objective To investigate the change and its clinical significance of plasma insulin and leptin level in the patients with cerebral infarction.Methods The plasma leptin, insulin, glucose levels and insulin sensitivity index(ISI) of 31 patients with atherothrombotic cerebral infarction (ACI), 30 patients with lacunar infarction (LI) and 21 cases of healthy controls were determined. Results Compared with those in the controls, there were elevation of plasma insulin level (6.17 ? 4.33 ?IU/ml, P

OBJECTIVE： To investigate the possible role of the alteration of mitochondrial transmembrane potentials(Δ Ψ m) in arsenic trioxide(As2O3)-induced apoptosis of human gastric adenocarcinoma cells(SGC7901).METHODS： Cell apoptosis was assessed through morphology， cell viability， the percentage of sub-G1 cells and phosphatidylserine(PS) externalization.the Δ Ψ m was detected on flow cytometry through double staining of Rhodamine 123 (Rh123) and propidium iodide(PI).RESULTS： The As2O3-induced apoptosis was associated closely with the disruption of the Δ Ψ m.CONCLUSION： The disruption of the Δ Ψ m is one of the mechanisms of As2O3-induced apoptosis.

Acute Lung Injury ; chemically induced ; pathology ; Animals ; Humans ; Lung ; pathology ; Nanospheres ; adverse effects ; Titanium ; adverse effects