Objective:To investigate the effect of Dan Sen on gastric mucosal Na +-K +-ATPase activity and gastric transmucosal potential difference in rats during severe intraperitoneal infection.Methods:The intraperitoneally infected rat model was established by cecal ligation and puncture(CLP).Assay of Na +-K +-ATPase activity in gastric mucosal tissue was conducted by biochemistry method.The electric-physiological recorder meter was used to measure gastric mucosal potential difference.Results:The activity of Na +-K +-ATPase markedly decreased in infected group at 3h after perforation,compared with the control group ( P

OBJECTIVE： To investigate the possible role of the alteration of mitochondrial transmembrane potentials(Δ Ψ m) in arsenic trioxide(As2O3)-induced apoptosis of human gastric adenocarcinoma cells(SGC7901).METHODS： Cell apoptosis was assessed through morphology， cell viability， the percentage of sub-G1 cells and phosphatidylserine(PS) externalization.the Δ Ψ m was detected on flow cytometry through double staining of Rhodamine 123 (Rh123) and propidium iodide(PI).RESULTS： The As2O3-induced apoptosis was associated closely with the disruption of the Δ Ψ m.CONCLUSION： The disruption of the Δ Ψ m is one of the mechanisms of As2O3-induced apoptosis.

Objective To investigate the imaging features in neonates with hypoglycemic brain injury by early and serial MRI.Methods Neonates who were admitted to neonatal department from May 2005 to Jul 2013 received MRI and diffusion-weighted imaging(DWI) scan within 7 days after hypoglycemia.Fortynine neonates were diagnosed with hypoglycemic brain injury.Thirty-four neonates received second MRI scan between 2 ～3 weeks after hypoglycemia.Seven neonates received third MRI scan.Results All the 49 neonates showed hyperintensity on DWI in the regions of occipital and parietal lobe for the first MRI scan(mainly involved 33 cases).Six cases combined frontal lobe and temporal lobe.Ten cases presented widespread cortex and white matter involvement.All the cases presented hypointensity on DWI for the affected area,T1 and T2 weighted image signal changes were not obvious.Some cases presented deep white matter and gray matter injury.Thirty-four cases received second MRI scan between 2 ～3 weeks after hypoglycemia,20 presented hypointensity on DWI,hypointensity on T1 weighted image and hyperintensity on T2 weighted image.Eleven cases with normal signals,and the other 3 were absorbing.Seven cases received third MRI scan,appeared encephalomalacia,myelin retardation,white matter volume decreased,hypoplasia of corpus callosum.The more severe the clinical symptoms was,the more severe the degree of brain injury showed.Conclusion Cerebral occipital and parietal regions are the most vulnerable in neonatal hypoglycemic brain injury.Early DWI for the imaging diagnosis of hypoglycemic brain injury should be taken within one week after hypoglycemia.Mild injury is recoverable,but severe would chang into necrosis and encephalomalacia.Some combined deep white and gray matter injury might related to hypoxia and ischemia.

Esophageal carcinoma represents high incidence and mortality rate in China.Irinotecan (CPT-11) has shown promising activity in a number of gastrointestinal cancers,including esophageal cancer.This article reviews recent clinical trials of irinotecan-based chemotherapy and radiochemotherapy in esophageal carcinoma.

Acute Lung Injury ; chemically induced ; pathology ; Animals ; Humans ; Lung ; pathology ; Nanospheres ; adverse effects ; Titanium ; adverse effects

Objective: To investigate the effect of growth hormone(GH) and hypo calorie enteral nutrition(EN) on protein metabolism in patients after cardiac operations. Methods: 36 patients after cardiac operations were randomly divided into two groups. The test group received GH and EN, and the control group received EN only. The treatment was maintained 7 days postoperatively. Plasma albumin(A)?transferrin(TF)?prealbumin (PA)?retinol binding protein(RBP) on the preoperative day and day 1,4,8 postoperatively and nitrogen balance(NB) on the day 1,2,3,5,7 postoperatively were detected. Results: There was no significant difference of all plasma protein levels between two groups before operation and all decreased on the 1st postoperative day, but only RBP higher in the test group than that in the control. There was significant difference in TF,PA,RBP levels on the 4th and 8th postoperative day, the plasma A level on the 8th postoperative day, and NB on the 1st to 7th postoperative day between the two groups. Conclusion: Combined use of GH and EN can elevate plasma A,TF,PA,RBP levels and improve negative NB.

Objective] To observe the clinical effects of Tianwang Buxin decoction in the treatment of heart Yin deficiency type of Generalized Anxiety Disorder. [Methods] 63 patients with Generalized Anxiety Disorder(heart Yin deficiency type) were randomly divided into three groups: Tianwang Buxin decoction group, paroxetine group, Tianwang Buxin decoction combined paroxetine group, course of treatment for 8weeks.Contrast the scores of three groups by HAMA before and after treatment. [Results] The clinical efficacy was almost the same among the three groups without significant difference, but the decrease of HAMA scores of Tianwang Buxin decoction combined paroxetine group was obviously lower than Tianwang Buxin decoction group and paroxetine group(P<0.05), among which, TCM group and combination group of TCM and WM had less side effect than WM group. [Conclusion]The clinical efficacy of Tianwang Buxin decoction group is almost the same with the paroxetine group without significant difference. The clinical efficacy of Tianwang Buxin decoction combined paroxetine group is more significant.

Objective To explore the effect of Buzhong Yiqi combined with low frequency impact feedback and rehabilitation training on postpartum pelvic floor rehabilitation.Methods100 cases of maternal fertility were randomly divided into observation group and control group, and 50 cases were randomly divided into observation group and control group.The control group was given low-frequency shock and electrical stimulation and rehabilitation training intervention, the observation group in the control group at the same time plus the addition of Buzhong Yiqi Tang treatment, were treated for 3 months.To evaluate and compare the pelvic floor muscle function, vaginal tension, vaginal pressure and nocturia before and after treatment, and compare the quality of life and the occurrence of complications after treatment.ResultsThere were no significant differences in the parameters of the pelvic floor muscle, vaginal tension, vaginal pressure and nocturia before treatment.and the maximal muscle voltage of the pelvic floor muscle and the pelvic floor muscle contraction were 60 s, the mean muscle voltage values of the pelvic floor muscle were significantly increased (P<0.05).However, the vaginal muscle tension and vaginal pressure in the observation group were significantly higher than those in the control group (P<0.05), and the vaginal muscle tension and vaginal pressure were significantly higher (P<0.05), but the number of nocturia in the observation group was significantly lower than that in the control group (P<0.05), and the FS-FI score of the observation group was significantly higher than that of the control group (P<0.05).The incidence of urinary incontinence was 4.26% and 6.38% in the observation group were significantly lower than those in the control group (16.67%, 18.75%, P<0.05).ConclusionBuzhong Yiqi Fang combined with low-frequency shock and electric stimulation, rehabilitation training treatment is conducive to promoting maternal postpartum pelvic floor rehabilitation, improve sexual quality of life, and can effectively reduce the incidence of pelvic organ prolapse, urinary incontinence.

Plasma cell mastitis,also known as periductal mastitis or mammary duct ectasia,always occurs in young women.Because of It's Complicated and varied clinical manifestations,it is easily misdiagnosed as breast cancer.Early diagnosis and staging helps to avoid unnecessary surgery.At present,There is no unified understanding to this kind of disease.Modern medicine mainly treated it by surgery while Traditional Chinese medicine believes it shoull be treated internally and externally.Complete resection of the lesion and the cosmetic effect is the focus of current research.This article reviews the progress of research in plasma cell mastitis etiology,pathology,diagnosis and treatment.

Objective To investigate the change and its clinical significance of plasma insulin and leptin level in the patients with cerebral infarction.Methods The plasma leptin, insulin, glucose levels and insulin sensitivity index(ISI) of 31 patients with atherothrombotic cerebral infarction (ACI), 30 patients with lacunar infarction (LI) and 21 cases of healthy controls were determined. Results Compared with those in the controls, there were elevation of plasma insulin level (6.17 ? 4.33 ?IU/ml, P