Objective In order to investigate the causes of postpartum hemonrrhage,and make clear the corresponding counter nursing measure. Methods The data of 103 patients with postpartum hemorrhage was selected and analyzed, the coresponding nmsing measures of the patients were summaried. Results The cur-ing rate of postpartum hemorrhage was 99.1%, the postpartum hemorrhage symptom greatly alleviated after abo-rative nursing. Conclusions The clinical intervention can reduce incidence of postparturn hemorrhage,in-crease the curing rate of it and lower the mortality rate of pregnant women.

Objective The purpose of the present study was to explore the use of psychological intervention in modified electroconvulsive therapy patients.Methods Divide all 160 patients random into two groups,the intervened group and the contrast group. The intervened group was accepted psychological intervention before the therapy day;The contrast group just did as normal.Record the anxiety, blood pressure,heart rate and the agreement to the therapy of the two group before the therapy.Results The anxiety of the intervened group was much little to the contrast group.The blood pressure and the heart rate of the intervened group was lower than the contrast group.Patient of the intervened group was much active to match with the treatment. Conclusions The use of psychological intervention in modified electroconvulsive therapy patients could decrease patient’s anxiety and painness,made them more active to match with the treatment.

To determine energy compounds in myocardial tissue simultaneously, we determined standard Creatine(Cr), Creatine phosphate(PCr), Hypoxanthine(Hypo), Nicotinamide adenine dinucleotide (NAD), Adenosine monophosphate (AMP), Adenosine diphosphate (ADP), Adenosine triphosphate (ATP), Cyclic-adenosine monophosphate(cAMP), and other compounds related to energy metabolism by a rapid isocratic method.The result showed that standard curves of the eight compounds were linear and passed through the origo for all examined concentrations (r=0.999).A recovery (88.1～117.6%) and replication (CV%=0.97～3.78%) for the present method was found to be accord with methology request.

Objective To study the clinical significance of cardiac enzymes of acute cerebral infarction(CI). Method The cardiac enzymes were determined in 92 patients with CI within 48 hours. The cardiac enzymes of CI group and control group,consciousness disturbance group and consciousness group were compared. Results The cardiac enzymes of CI were evaluated than the control group (P

The preventive effects of starch-Fe and sucrose-Fe on iron deficiency anemia were investigated. Weaning rats were used as experimental animal, and divided into starch-Fe, sucrose-Fe, ferrous sulfate and control group. The former 3 groups were set for the Fe deficiency preventive group and each animal received elemental iron 5-6mg per kg body weight per day according to its preparation demand orally. The control group was given ordinary diet only. After 24-day feeding with its experimental diet serum iron(SI), serum ferritin (SF) and hemoglobin (Hb) of the animal were measured. The results revealed that SI (?g/dl) was 111.08 ? 22.30,118.64?26.80, 136.81?54.36 and 83.44?22.60; SF (ng/ml) was 12.69?3.19, 14.52?1.98, 16.91?2.02 and 7.51?2.09; Hb (g/dl) was 10.71?0.63, 10.96 ?0.45, 11.01?0.84 and 8.46 ? 1.27 respectively. This suggested that the starch-Fe and sucrose-Fe could be well absorbed and probably favorable to rescue children from iron deficiency anemia.

Objective To evaluate the capacity of air quality test of indoor air quality test agencies in Beijing. Methods 59 laboratories participated the evaluation. The standard reference (SRF) material, ammonia and benzene, were used as the test items and the national standard methods were used in the determination, the capacity evaluation was done by comparing the test values and Robust Z-Score. Results 28 laboratories (47.5%) presented a satisfactory result for both ammonia and benzene determination, 3 gave an unsatisfactory result for both items, 28 showed an unsatisfactory result or a result with some error in alternative1 item. Conclusion In the present study, only 47.5% of the investigated laboratories are satisfied. There are still some problems in the capacity of air quality test of test agencies in Beijing and the test capacity need to be improved.

Objective:To compare the effects of different anesthesia methods on stress hormones and hemodynamic changes in the patients with gynecological laparoscopic surgery. Methods:Retrospective analysis was carried out. Totally 145 patients with gynecolog-ical laparoscopic surgery were divided into two groups according to the anesthesia method, the control group with 72 cases was given routine remifentanyl propofol intravenous anesthesia, and the observation group with 73 cases was given routine propofol isoflurane com-posite anesthesia. Such hemodynamic indices as SBP, DBP and HR, and plasma NE, E, cort and AT II levels at different time points in the two groups were studied and compared, and adverse drug reactions were also observed in the two groups. Results:The BP and HR at T2 and T3 time point in the two groups were significantly lower than those at T0 time point (P<0. 05), and those in the obser-vation group were lower than those in the control group at the same time point(P<0. 05). The levels of NE, E, cort and ATⅡat T2 and T3 time point in the two groups were significantly higher than those at T0 time point (P<0. 05), while the increase in the observa-tion group was lower than that in the control group (P<0. 05). The incidence of adverse drug reactions (ADR), such as respiratory depression, hypertension, bradycardia and so on, and the total incidence of ADR in the observation group were both lower than those in the control group (P<0. 05). Conclusion:Compared with remifentanyl propofol intravenous anesthesia, propofol isoflurane composite anesthesia can keep the hemodynamic stability more effectively and reduce the stress reaction with fewer ADR and promising safety, which is worthy of wider application in clinics.

Objective To study the pharmacological effects of Qinggong Decoction on sedation and hypnosis. Methods Fifty mice were randomly divided into five groups: NS group, diazepam group, and low-, mid-, high-dose Qinggong Decoction group. Diazepam group was given 0.1 mL/10 g diazepam, and other four groups were given 0.2 mL/10 g corresponding drugs. Spontaneous activities before and after ig. were recorded and the sleeping time of mice with pentobarbital sodium (superthreshold dosage) and the number of the sleeping mice with pentobarbital sodium (subthreshold dosage) were measured. Convulsion model induced by nikethamide was used to observe the change of the seizure rate in mice after given Qinggong Decoction. Results Qinggong Decoction (3.0, 6.0 g/kg) could obviously inhibit spontaneous activities of mice, prolong the sleeping time of mice with pentobarbital sodium (superthreshold dosage) and increase in the number of the sleeping mice with pentobarbital sodium (subthreshold dosage), and had markedly anticonvulsant effect in mice induced by nikethamide. Conclusion Qinggong Decoction has obvious effects of sedation, hypnosis and anticonvulsion.

Objective To understand the quality of life in patients with chronic obstructive pulmonary disease (COPD) ,and to analyze the influencing factors for their quality of life .Methods A total of 250 patients with COPD from 3 hospitals in Zhanjiang City were investigated by using the Chinese Scale Of World Health Organization (WHOQOL‐BREF) ,and the influencing factors for quality of their life were analysed ,as well .Results A total of 237 qualified questionnaires were reclaimed out of 250 questionnaires , and the response rate was 94 .80% .Scores of physiological aspect ,psychological aspect ,social relation ,environmental condition and comprehensive health self evaluation were (59 .57 ± 15 .73) ,(56 .99 ± 13 .01) ,(64 .28 ± 14 .82) ,(58 .70 ± 11 .53)and (74 .97 ± 12 .61) ,respectively .The scores of quality of life in the physiological aspect ,psychological aspect ,social relation and environmental condition of male patients with COPD were higher than those of the female ;the scores of quality of life in the social relation in pa‐tients with higher education level were higher than that in patients with lower education level ,the scores of quality of life in the physiological aspect ,psychological aspect ,social relation and environmental condition in patients with higher income level were high‐er than those in patients with lower income level ;the disease severity was higher ,the scores of quality of life in the physiological as‐pect ,psychological aspect ,social relation and environmental condition were lower ,all had statistically significant differences (P<0 .05) .Logistic regression analysis showed that sex ,monthly income level and disease severity were the influence factors of quality of life self evaluation in COPD patients .Conclusion The quality of life in patients with COPD might be influenced by sex ,income level ,level of education and disease severity .It is necessary to strengthen the health education ,pay attention to personalized care and improve the quality of life of patients with COPD .

Objective To clone the human target gene HBVDNAPTP1 transactivated by hepatitis B virus DNA polymerase obtained by screening with suppression subtractive hybridization (SSH) and bioinformatics techniques. To construct prokaryotic expressive vector of HBVDNAPTP1 gene, induce the expression of recombinant protein in E. coli, and analyze the expression level of HBVDNAPTP1 gene in human tissues. Methods The DNA fragment of HBVDNAPTP1 was amplified by reverse transcription polymerase chain reaction (RT-PCR) taking mRNA from HepG2 cells as the template, and the correct DNA fragment was then inserted into inducible prokaryotic expressive vector pET-32a (+). The competent BL21 (DE3) E. coli was transformed, and then cultured and induced with IPTG. The expressed HBVDNAPTP1 was confirmed with Western blot. UniGene database was used to analyze the chromosome mapping and tissue expression profile of HBVDNAPTP1 gene. Results The DNA fragment of HBVDNAPTP1 was amplified by RT-PCR. HBVDNAPTP1 expressive vector was constructed. After transformation with pET-32a(+)-HBVDNAPTP1 and induction with IPTG, recombinant HBVDNAPTP1 was expressed and confirmed by Western blot. The expression of genomic location of HBVDNAPTP1 gene was low in multiple-tissues with the exception of pituitary gland, tonsil, tongue, thymus, trachea and umbilical cord. Conclusion The recombinant HBVDNAPTP1 gene could be expressed in prokaryotic expression system of E. coli. The chromosome mapping and tissue expression level of HBVDNAPTP1 gene is tentatively conceived.