Objective:To analyze the reasons of complicated arrhythmia after resection of lung. Methods:The relation of arrhythmia with age, operation mode,preoperative lung function and postoperative blood gas of 562 cases of lung resection were analyzed. Results:There were 104 of 562 cases (18.5%) complicated with arrhythmia postoperatively. 38.77% of them were older than 70 years. 27.72% had operation of pneumonectomy.The parameters of FEV 1,MVV,FVC lower than 50% were 67.24%,46.53% and 67.93% respectively.Postoperative blood gas analysis of the 23 cases showed that Po 2 was lower than 60 mmHg,Pco 2 was higher than 45 mmHg,So 2 was lower than 96%.21 of them developed postoperative arrhythmia.After tracheotomy and immediately correcting anoxia,all the 21 patients recovered from arrhythmia within 3 days.4 patients died in this group(3.84%).Conclusion:Pneumonectomy,in patients older than 70,results in the parameters of FEV 1,MVV and FVC lower than 50%.Significant anoxia is the cause of arrhythmia.

Objective To comparatively analyze the results of the PT derivative method and the Clauss method for detecting fi-brinogen(Fib) by the automated coagulation analyzer and to explore their accuracy and clinical value .Methods Fib was detected in 156 blood samples selected from the patients in this hospital from March to May 2013 ,25 normal control samples and 13 positive samples of D-dimer (DD) by the TP derivative method and the Clauss method .Results The Clauss method and the PT derivative method by Sysmex CA-1500 coagulation analyzer had good repetitive rate in measuring Fib for 25 times ;when Fib<1 .0 g/L ,the results detected by these two methods had statistically significant difference (P<0 .05);when Fib in the range of 1 .0 -2 .0 g/L , 2 .1-3 .0 g/L ,3 .1-4 .0 g/L ,the detection results had no statistically significant difference between the two methods (P>0 .05);when Fib in the range of 4 .1 -5 .0 g/L ,the detection results had statistically significant difference (P<0 .05);when Fib>5 .0 g/L ,the detection results had statistically significant difference (P<0 .05);in 13 DD positive samples ,the detection results of the PT derivative method were significantly higher than those of the Clauss method with statistically significant difference (t=8 .530 , P<0 .05) .Conclusion The difference exists between the PT derivative method and the Clauss method for detecting Fib ,but the ac-curacy of the Clauss method is higher than its specificity ,the Clauss method should be the first choice for the clinical determination of plasma Fib .

OBJECTIVE: To observe the effects of long-term administration of pioglitazone at different doses on the expression of glucose transporter-4(GLUT-4) of cardiac muscle tissue in rats and to discuss the possible mechanisms.METHODS: A total of 40 Wistar rats were enrolled,of which,10 were randomly assigned to normal control group(NC),and the other 30 were injected intraperitoneally with streptozotocin to establish diabetes mellitus(DM) rat model.After successful modeling,the rats were assigned to DM model group(DM,normal saline),high and low dose pioglitazone group(HP,LP;0.75,0.25 mg?kg-1?d-1).The NC groups received normal saline for 12 weeks.Blood glucose(BG) level before grouping(baseline level) and prior to death was determined by tail-cutting method.GLUT-4 level in cardiac muscle tissue and both protein and mRNA expression of AMPK? were determined by RT-PCR and western blot,respectively.RESULTS: As compared with NC group,the BG level in DM group was significantly increased but the expression of GLUT-4 and AMPK? decreased significantly(P

Objective To establish a Chromatin immunoprecipitation assay to analyze transcriptional regulation of gene expression in hepatocytes. Methods The PEPCK gene was used as a target gene, HPRT, MBP and IgG were used as internal reference, the chromatin immunoprecipitation and PCR were performed with antibody specific for CREB to verify bindings of transcription factor CREB to the PEPCK promoter under various metabolic conditions. Results Transcription factor CREB occupies its target sites in the PEPCK promoter and this binding is increased by 4-fold during fasting in the anti-CREB antibody immunoprecipitated DNA fragments. Conclusion The results confirm that CREB binds to the PEPCK promoter and is involved in transcriptional regulation of PEPCK gene in hepatocytes.

Objective:To discuss the method of Video Assisted Thoracoscopy(VATS) in diagnosis and treatment of small peripheral lung neoplasm.Methods:A wedge-shaped excision with VATS had been performed on 20 patients with small peripheral lung neoplasm.The sample had been sent to pathology during surgery.Lobectomy was done with the malignant tumor.Results:14 cases(70%) were malignant in stage IA ,6 cases were benign.The mean follow-up time postoperatively was 24 months(12～36months),there was no relapse case.Conclusion:For those patients,the diameter of neoplasm is smaller than 15mm,and antibiotic treatment is ineffective for 2～3 weeks,VATS can be available for diagnosis and treatment.

Objective:To explore protective mechanism of buflomedil Hydrochloride on the neuron of damaged Sciatic nerve in rats. Methods:SD rats were divided into normal, model,buflomedil hydrochloride group in random. Preconditioned for 14 days,the sciatic nerve lesion models were performed, the Bcl-2 and Bax positive cells were counted by immmunohistochemistry at the end of 6h,12h,24h,48h,72h,96h,7days respectively. Results:(1) At the end of 12h after the model perfomed,The expressions of Bcl-2 in buflomedil hydrochloride group increased obvionsly than the normal and model groups,peaked at 24~48h,and at the end of 7days,the expressions of Bcl-2 in buflomedil hydrochloride group increased obviously compared with model group.(2)The expressions of Bax in buflomedil hydrochloride groug were lower than the normal and model groups in the same periods,and that was the lowest at the end of 7 days,It was different significantly than that of model group(P

Monitoring data of antibiotic susceptibility from both home and abroad showed that carbapenem-resistant Enterobacteriaceae (CRE) are being on the rise,which represents an increasingly urgent clinical danger and an emerging public health concern worldwide.In order to effectively prevent CRE transmission in healthcare settings,we should not only pay high attention to the capability building of the laboratory of clinical microbiology,but also actively explore into the mechanisms of carbapenem resistance in Enterobacteriaceae.What's more,we should substantially raise the detection capacity of laboratory on CRE,effectively carry out the antimicrobial stewardship program,and strictly implement hand hygiene and contact precautions.(Chin J Lab Med,2013,36:300-302)

Based on 10 years management experience of CMA-1,OREAL China skin/hair grant,the article puts forward three main factors of scientific funds management,which are:adhereing to the fair and justice review,constantly strengthening the service consciousness and improving the service level,constantly perfecting the management system.The article also summarizes some specific experience and practice,including:Chinese and French experts joint review,avoid principle and double-blind review,Service provider role orientation,internet management platform development and whole process management,etc

Objective To discuss the effect of the PDCA cycle in enhancing the case care ability of nurses in order for nurses to adapt to the assessment model of cases tracking.Methods The training was carried out for enhancing nurses' ability of case care according to the PDCA cycle.Results The scores of the case care,the quality of care in wards and the patient satisfaction degree increased significantly after applying the PDCA cycle compared with those before.The aims of ensuring medical safety,improving the work quality,and increasing patient satisfaction were achieved.Conclusions Training nurses' ability of case care following the PDCA cycle can effectively improve the nurses' ability of case care,make them adapt to the case tracking assessment model,which is worthy of promotion.

BACKGROUND:Cytomegalovirus is one of the opportunistic infection viruses for organ transplant patients, and it can lead to functional loss of organ grafts and even endanger lives. Therefore, accurate diagnosis of cytomegalovirus infection at early phase is the key to clinical treatment. OBJECTIVE:To analyze the detection method, accuracy, detection time and clinical significance of cytomegalovirus infection after organ transplantation. METHODS: A computer-based search of VIP, PubMed and CNKI as wel as manual search of books were performed for literatures concerning organ transplantation and cytomegalovirus infection published from January 2007 to December 2014. The keywords were transplantation, cytomegalovirus in English and Chinese, respectively. Total 1 894 papers in English and 31 articles in Chinese were found. Among them, only 35 papers which were highly representative and published in related authoritative journals were chosen for further analysis. RESULTS AND CONCLUSION:The main non-invasive detection of cytomegalovirus infection is stil the test of CMV-pp65 antigen with good sensitivity and specificity which can accurately reflect the status of cytomegalovirus infection. This method is also the most important clinical diagnostic method. Molecular biological detection of cytomegalovirus can detect potentialy infected people, and this method can detect the infection earlier than CMV-pp65 antigen method, which is suitable for the early diagnosis of cytomegalovirus infection and early drug treatment. Quantitative nucleic acid test can be used to analyze cytomegalovirus-DNA copies so as to determine the concentrations of the virus in patients, which may provide a more sensitive monitoring way for inapparently infected patients. Cytomegalovirus-IgG and cytomegalovirus-IgM in serum can be used to determine whether organ transplant recipients have been infected by cytomegalovirus. Virus culture and histological examination have been used for several years, both of which are the gold standard for detecting cytomegalovirus. However, these methods cannot be used in early diagnosis and active infection. Immunological detection method provides a new ideal for the diagnosis of cytomegalovirus infection. This method can monitor cytomegalovirus infection from early phase accurately and assess the risk of cytomegalovirus, providing a new way for the development of the diagnosis of cytomegalovirus infection.