Objective To compare the quality of platelet concentrates (PCs) between two different flow speeds of leukodepletion through filtration processes.Methods MCS+version C5 and disposables were used,each of which linked with a leuko-depleted filter of LRF series products,The PCs was gone respectively through a continuous slow filtration process at flow rate about 5 to 8 ml per minute in collection process (L-LDP) and through a fast filtration process at flow rate about 25 ml per minute after collection (F-LDP). The anticipation for PCs yield was ≥2.5?10 11 cells/bag and for residual white blood cells (WBC) was≤5.0?105 cells/bag.Results The PCs products obtained by L-LDP and F-LDP correspondingly showed the mean platelet yield (2.70?0.29)?10 11 cells/bag and (2.56?0.38)?10 11 cells/bag (t=1.25) the residual WBC (7.73?0.01)?104 cells/bag and (2.02?3.05)?105 cells/bag (log t=1.48), the success collection rate 78.6% and 70.6%, as well as the collection efficiency 57.1?6.8% and 55.5?8.7% (t=0.64) against precount. As both anticipated criteria of platelet yield and residual WBC level to be met synchronously, the PCs for L-LDP accounted for 78.6% (22/28) and the one for F-LDP hand only access to 64.7% (11/17).Conclusion The satisfactory results for PCs were obtained through both different leukodepleted filtration processes. As all individualiged indexes observed of L-LDP excelled that of F-LDP, the PCs filtrated at low speed had a better quality than the one at fast speed.

Rubus corchorifolius(Shanmei or mountain berry,2n=14)is widely distributed in China,and its fruits possess high nutritional and medicinal values.Here,we reported a high-quality chromosome-scale genome assembly of Shanmei,with contig size of 215.69 Mb and 26,696 genes.Genome comparison among Rosaceae species showed that Shanmei and Fupenzi(Rubus chingii Hu)were most closely related,followed by blackberry(Rubus occidentalis),and that environmental adaptation-related genes were expanded in the Shanmei genome.Further resequenc-ing of 101 samples of Shanmei collected from four regions in the provinces of Yunnan,Hunan,Jiangxi,and Sichuan in China revealed that among these samples,the Hunan population of Shanmei possessed the highest diversity and represented the more ancestral population.Moreover,the Yunnan population underwent strong selection based on the nucleotide diversity,linkage dise-quilibrium,and historical effective population size analyses.Furthermore,genes from candidate genomic regions that showed strong divergence were significantly enriched in the flavonoid biosyn-thesis and plant hormone signal transduction pathways,indicating the genetic basis of adaptation of Shanmei to the local environment.The high-quality assembled genome and the variome dataset of Shanmei provide valuable resources for breeding applications and for elucidating the genome evo-lution and ecological adaptation of Rubus species.