Objective To explore the effects of antagonist of angiotensin II(Ang II) receptors on acute pancreatitis(AP) of rats. Methods The Sprague-Dawley rats were divided randomly into normal group, AP group, AP+lower dose of valsartan(5mg/kg) group , AP+higher dose of valsartan(8mg/kg) group, lower dose of valsartan group and higher dose of valsartan group, and each group containing 6 animals. AP animal models were induced by intraperitoneal injection of cerulein. Valsartan was given by intragastric administration. The histopathological changes of pancreas were observed and scored, and the pancreatic edema was evaluated. The levels of plasma amylase (AMY) and pancreatic myeloperoxidase (MPO) were measured. Results Compared with AP group, the pathologic lesions of pancreas and pancreatic edema decreased in the group AP+ valsartan, and the levels of plasma AMY and MPO also reduced. Conclusion Pretreatment with antagonist of angiotensin II receptors in rat acute pancreatitis can markedly reduce pancreatic injury and inflammation.

Child ; Humans ; Pulmonary Embolism ; diagnosis ; etiology ; therapy ; Risk Factors

To investigate the relationship between diabetic macular edema (DME) and serum E-selectin level.
●METHODS: Totally 90 patients with diabetic retinopathy in our hospital from October 2009 to October 2013 were observed. Retrospective analysis of the correlation between macular edema degree and serum soluble E -selectin ( sE - sel ). Ninety patients with diabetic retinopathy were divided into two groups: DME group and non - DME group. The level of sE - sel and microalbuminuria (MA) of two groups were determined and compared.
●RESULTS: There was significant difference in MA, sE-sel levels of groups ( P < 0. 05). The value of diabetic retinopathy patients with sE - sel and MA, FBG were positively correlated ( r = 0. 728, P < 0. 05; r = 0. 651, P <0. 05).
●CONCLUSlON: Elevated levels of sE-sel is a risk factor for diabetic macular edema, and sE-sel was involved in the pathogenesis of diabetic microangiopathy.

Objective To study the relationship between the microvessel damage and the death rate of tumor cells and determine role of microvessel damage in the tumoricidal effect of hyperthermia.Methods H22 hepatoma cells were inoculated in the right hind legs of KM mice with immunosuppression.Local hyperthermia was administered to these mice which were divided randomly into 10 groups (8 in each group),which included groups of 3,6,12,24,48,72 and 96 h after hyperthermia for 30 min and groups of hyperthermia for 15,30 and 45 min.The tumor tissues were analyzed by flow cytometry(FCM)and immunohistochemistry.The cultured H22 cells were also given hyperthermia for 15,30,45 min and analyzed by FCM.Results The apoptotic rate(0.115±0.008)was higher in the initiation phase of hyperthermia in vitro,then the necrotic rates rose gradually.The difference between in vivo and in vitro was significant in hyperthermia of the same time about death rate(P＜0.05).A strong positive linear correlation(r=0.844)was observed between the death rate of tumor cells and MVD.Conclusion Microvessel damage plays the cardinal role in tumoricidal effect of hyperthermia.

Objective To explore the application of experienced teaching method in the teaching of Fundamentals of Nursing.Methods The feasibility and validity of experienced teaching method was evaluated by expounding the utilization and result of role-play method and practice experienced method.Results The experienced teaching method received good result and proved appropriate in the teaching of Fundamentals of Nursing.Conclusion The experienced teaching method overcame the disadvantages of mere traditional teaching method and aimed at the improvement of quality and ability of students.It contributed to developing the critical thinking ability and other abilities of students.

ObjectiveTo observe the chronological and spatial expressions of transforming growth factor-β receptor typeⅡ(TGF-βRⅡ)and fibronectin (Fn )in developing mouse kidney and investigate the relationship between the expressions and kidney development. Methods Immunohistochemistry and Western blotting were used to observe and measure the expression of TGF-βRⅡ and Fn in embryos and postnatal mouse kidneys. Results The expression of TGF-βRⅡ was noticed in all stages of glomeruli, renal tubes and collecting ducts, and increased gradually with ages; The expression of Fn was noticed too in all stages of glomeruli, renal tubes and collecting ducts, except for the comma-shaped bodies, and also increased gradually with ages. Conclusion TGF-β and Fn might play an important role in the development and maturation of mouse kidney.

BACKGROUND:Femoral neck fracture combined with femoral shaft fracture as a high-energy injury exhibits some difficulties in its clinical treatment because of bone chips and bone defects. Bone and cel transplantations have been used recently in order to effectively fil the bone defect area. OBJECTIVE:To explore the therapeutic effect of bone transplantation versus cel transplantation for treatment of femoral neck fracture combined with femoral shaft fracture. METHODS: A retrospective analysis was performed in patients with femoral neck fracture combined with femoral shaft fracture, who respectively underwent internal fixation with bone transplantation, tissue transplantation and cel transplantation. Perioperative indexes, incidence of complications and Harris scores were observed and compared. RESULTS AND CONCLUSION: (1) Femoral neck fractures are most commonly classified into Garden type, AO type and Pauwel type. Fracture classification aims to unify standards, which is easy to guide treatment and scientific research. (2) Internal fixation with bone transplantation and tissue transplantation is easy, safe and effective to promote fracture healing and improve hip function, which is an effective way to repair femoral neck fractures with femoral shaft fractures. (3) Compared with autogenous bone graft, alograft transplantation materials as finished products have a variety of sources, and have indications and efficacy similar to non-vascularized bone graft, which are suitable for clinical practice, but long-term effects need to be furtherexplored. (4) Internal fixation with cel transplantation is simple, reliable and effective for femoral neck fractures with femoral shaft fractures, which provides a new idea to reduce the early incidence of femoral head necrosis.

Metastasis to liver is the most happened distant metastasis and is the leading cause of death in patients with colorectal cancer.Complete surgical resection of liver metastases from colorectal cancer is major curative method for resectable metasrases.Other methods such as chemotherapy, targeted therapy, hepatic ar-tery intervention, radiotherapy, radiofrequenly ablation or cryo-ablation should be considered.Both systemic and local treatment are.important for liver metastasis from colorectal cancer.

Objective To observe the characteristics of different blood perfusion in renal cell carcinoma by contrast-enhanced ultrasound and prove its diagnostic value.Methods One hundred and six cases of renal cell carcinoma confirmed by patholog were involved,and were divided into three groups:clear cell carcinoma group,papillary cell carcinoma group,and chromophobe cell carcinoma group.Their imaging characteristics were observed.Time-intensity curve was analyzed by using QLab software.The difference of time of beginning to increase (△AT =ATrenal cell carcinoma-AT surrounding normal renal parenchyma) and the difference of peak intensity(△PI =PIrenal cell carcinoma-PIsurrounding normal renal parenhyma) in three groups were compared.Results △AT and △PI in clear cell carcinoma group were different from those in the papillary cell carcinoma group and chromophobe cell carcinoma group (P ＜0.05).The difference between papillary carcinoma group and chromophobe carcinoma group was not significant (P ＞0.05).Contrast agent filled very fast in most clear cell carcinomas,filled speed and time to peak were early or synchronously with the surrounding renal tissue.Compared with renal papillary carcinoma and chromophobe carcinoma group,clear cell carcinoma group showed a high peak intensity and part of the surroundings appeared high increasing signs,like of a ring.Contrast agents filled slow in papillary carcinomas group and chromophobe carcinomas group,and the peak intensities were low.Conclasions Contrast enhanced ultrasound can provide important clinical value for the diagnosis of renal cell carcinoma and subtype diagnosis.

OBJECTIVE:To optimize the formulation of Asiaticoside cationic liposomes,and to investigate the characteristics of drug release in vitro. METHODS:The thin film dispersion method was used to prepare liposome;using encapsulation efficiency and drug-loading amount as index,the formulation of Asiaticoside liposomes was optimized by central composite design-response surface method with the ratio of drug to lipid(X1),the ratio of cholesterol to lipid(X2)and the concentration of D-mannose(X3) as factors. Using sodium lauryl sulfate as medium,in vitro release characteristics of cationic liposomes prepared with 1%octadecyl-amine was investigated by bag filter method,and compared with those of Asiaticoside solution and common liposome. RESULTS:The optimal formulation was X1 0.07,X2 0.17 and X3 0.03 g/ml. The encapsulation efficiency was (75.529 ± 1.071)%,and drug-loading amount was(2.539±0.029)%(n=3);the deviation from the predicted values were -0.217% and 0.205%;1% oc-tadecylamine was add into formulation to obtain cationic liposomes,and the Zeta potential had changed from -5.6 mV to 20.8 mV. in vitro accumulative release rates of Asiaticoside solution,common liposomes and cationic liposomes were 89.13%(12 h), 87.58%(72 h) and 94.46%(72 h),and the latter was in line with Weibull model. CONCLUSIONS:Asiaticoside cationic lipo-somes have high encapsulation efficiency,and can releases for 72 h.