Objective To investigate the analgesic effect of warm needling and explore its relationship with the function of mast cells in the acupoint area and the specificity of acupoints.Method Sixty SD rats were randomized into blank (C), model (M), warm needling Zusanli (Z), contralateral warm needling (O), manual acupuncture (A), warm needling Futu (F), warm needling Yanglingquan(Y), warm needling Weizhong (W), disodium cromoglycate + warm needling (DSCG+Z) and saline + warm needling (Saline+Z) groups. A rat with adjuvant arthritis was used as a model of inflammatory pain (AA model). Hindpaw withdrawal latency was measured in therats during warm needling at Zusanli (ST36), Yanglingquan (SP9), Futu (ST32) and Weizhong (BL40). Pre-treatment and post-treatment rates of mast cell degranulation in the acupoint area were compared and the effect of acupoint area injection of disodium cromoglycate on the degranulation was observed by acupoint tissue section staining.Result Obvious degranulation of mast cells in the acupoint area appeared after warm needling (P>0.05). The analgesic effect of warm needling was better than that of manualacupuncture (P>0.05). After treatment, pain threshold was significantly higher in the warm needling Zusanli group than in the warm needling Futu, warm needling Yanglingquan and warm needling Weizhong groups (P>0.05). Acupuncture-induced mast cell degranulation could be prevented by disodium cromoglycate (P>0.05).Conclusion Warm needling at point Zusanli can produce an analgesic effect in adjuvant arthritis rats. Its analgesic effect is better than that of manual acupuncture. The rate of mast cell degranulation in the acupoint area is higher in the warm needling Zusanli group than in the manual acupuncture group. The analgesic effect of warm needling is reduced after pretreatment with disodium cromoglycate, a mast cell stabilizer. In an AA model, the analgesic effect of warm needling at point Zusanli is better than those of warm needling at points Yanglingquan, Futu and Weizhong.

Objective:To explore the relationship between parental rearing pattern and subjective quality of Life in children with asthma.Methods:Apply Egma Minnen av Bardndosnauppforstran(EMBU)and Inventory of Subjective Life Quality for children and adolescent(ISLQ)which to evaluate asthmatic children(mild 58,moderate 40)and 189 healthy children which age 9 to 12 years old.Results:the degree of father and mother's warm rearing pattern in mild and moderate asthmatic children(mild:45.7?8.3/44.0?9.2,moderate 52.1?9.3/50.0?11.2)were lower than that in health children(52.3?9.3,60.1?9.3,P

Objective To investigate the therapeutic effect of acupuncture combined with Vitalstim electric stimulation therapy for treatment of patients with dysphagia after ischemic stroke.Methods Ninety-two patients with dysphagia after ischemic stroke in accord with the criteria of enrollment in this study were admitted to the Department of Acupuncture of the First Affiliated Hospital of Tianjin Traditional Chinese Medicine University from May 2014 to November 2016, and they were divided into a control group and an observation group by random number table, 46 cases in each group. Both groups were treated with stroke conventional drug therapy and feeding swallowing rehabilitation training, in addition, Vitalstim swallowing therapeutic electrical stimulation therapy was used in the control group, and acupuncture combined with Vitalstim stimulation were applied in observation group. The changes of swallowing ability score, video fluoroscopic swallowing study (VFSS) score and adverse reactions were observed before and after treatment in the two groups, and the curative effect of combined method was evaluated.Results Compared with those before treatment, the swallowing ability and VFSS score of two groups after treatment were obviously higher, and the degrees of changesin observation group were more significant than those in control group (swallowing score: 8.12±1.13 vs. 7.45±1.04, VFSS score: 7.97±1.05 vs. 5.67±0.88, allP < 0.05); the total efficiency in the observation group was markedly higher than that in the control group [95.7% (44/46) vs. 78.3%(36/46)], the adverse reactions (wrong aspiration, pulmonary infection) in the observation group were significantly lower than those in the control group [10.9% (5/46) vs. 28.3% (13/46)], and there were statistically significant differences between the two groups (bothP < 0.05).Conclusions Acupuncture combined with Vitalstim electric stimulation therapy can improve the swallowing ability in patients with dysphagia after ischemic stroke, and the therapy has less adverse reactions; compared with the simple Vitalstim electric stimulation therapy, in the aspect of improving swallowing ability, the therapeutic effect of combined therapy is more significant.

Kawasaki disease is an autoimmune vasculitis disease, mainly involving small and medium-sized arteries, especially coronary arteries.The severity of coronary artery lesions is closely related to the prognosis of Kawasaki disease.Statins have been proved effective in the treatment of cardiovascular diseases.Recent studies have demonstrated the critical role of statins in the treatment of Kawasaki disease and their potential value in preventing the formation of coronary artery aneurysms in the acute and convalescent stages of Kawasaki disease.In this article, the research progress of statins in Kawasaki disease was reviewed.

Aim To investigate the protective effect of resveratrol on oxidative stress injury of cardiomyocytes induced by high fat and its correlation with AMPK/mTC)RCl/p70S6K signaling pathway. Methods The model of cardiomyocyte lipotoxicity injury was established by palmitic acid (PA). Resveratrol pretreatment and MTT assay were used to detect cell proliferation. The expression of reactive oxygen species ( ROS) was detected by fluorescence and flow cytometry, and the levels of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) were detected by kit. The protein expression of AMPK/mTORCl/P70S6K signaling pathway and apoptosis related proteins were determined by Western blot. Results MIT results showed that the cell viability decreased significantly at 0. 4 mmol L-1 and 24 h-48 h stimulation time, re-spectively. Fluorescence and flow cytometry showed that the levels of ROS and MDA increased significantly and SOD activity decreased significantly when PA (0. 4 mmol • L"1) stimulated cells for 24 h compared with control group. Western blot showed that the expression of p-AMPK and Bcl-2 decreased and the expression of p-mTORCl, p-p70S6K, Bax and cleaved caspase-3 increased when PA (0.4 mmol • L"1) stimulated cells for 24 h compared with control group. Resveratrol pre-treatment could significantly reverse the above chan-ges. Conclusions Resveratrol can significantly inhibit the apoptosis of cardiomyocytes induced by high fat. Its mechanism may be related to the regulation of AMPK/mTORCl/p70S6K signaling pathway.

BACKGROUND: Both cyclodextrin high polymer and polyvinyl alcohol (PVA) have been widely applied as biomedical materials owing to their characteristics including no toxicity and good biocompatibility and mechanical property and have become important functional materials of biological drug manufacture and medication research.OBJECTIVE: To observe the drug controlled release property of β-cyclodextrin (β-CD) loaded onto PVA by the covalent bond and to investigate the possible mechanisms of action. DESIGN, TIME AND SETTING: An observational experiment was performed at the Laboratories of Applied Chemistry, College of Science, Northwestern Polytechnical University, and College of Chemistry & Chemical Engineering, Xiangyang Normal University in 2008. MATERIALS: PVA (Tianda Experimental and Chemical Plant, Tianjin, China), β-CD (Sanpu Chemical Engineering Co.,Ltd., Shanghai, China) and camptothecin (Modern Times Chemical Institute, Xi'an, China) were used in this study. METHODS: Mono-6-O-tosyl-β-CD and mono-6-formyl-β-CD were synthesized separately. Silylatedβ-CD was loaded onto polymer chain of PVA by acetalization to produce PVA containingβ-CD (PVA-β-CD) linear high polymer. The clathration between PVA-β-CD and camptothecin was observed. Drug-release content of PVA-β-CD membranes under different pH values was determined through the use of ultraviolet-visible spectrophotometer. MAIN OUTCOME MEASURES: Synthesis condition and drug release rate of PVA-β-CD. RESULTS: The best condition for synthesis of PVA-β-CD linear high polymer comprised reaction time 2 hours, temperature 70 ℃, and the mass ratio of mono-6-formyl-β-cyclodextrin to PVA not higher than 4:1. Experimental results regarding drug release revealed that PVA-β-CD promoted water-insoluble drug release owing to its clathration and solubilization. Withβ-CD content increasing, camptothecin release amount and release velocity had no significant change in pH 11 medium but increased greatly in pH 2 medium. CONCLUSION: As for compact PVA membrane, addition ofβ-CD possibly leads to pore formation, thus promoting water molecule infiltration and drug diffusion, beneficial to drug release, and simultaneously, the solubilization of β-CD plays an important role in promoting the release of insoluble drug.

AIM:To investigate the role of JAK-STAT pathway , IL-1βand IL-6 in the PC12 cells with X-ray irradiation.METHODS:The PC12 cells were irradiated with X-ray at doses of 2, 4 and 8 Gy.After 24 h, the levels of IL-1βand IL-6 were detected by ELISA .The protein levels of p-JAK1, p-JAK2, p-STAT1, p-STAT3 and p-STAT5 were measured by Western blot .RESULTS:Compared with control group , the levels of IL-1βand IL-6 increased .The protein levels of p-JAK1, p-JAK2, p-STAT1, p-STAT3 and p-STAT5 increased with the doses of X-ray exposed.CONCLUSION:JAK-STAT signaling pathway , IL-1βand IL-6 play a role in the injury of PC12 cells with X-ray irradiation.

Apolipoprotein A-I (apoA-I) is a member of the apolipoprotein A family, which was discovered the earli-est. It has an important role in the regulation of lipid metabolism, which mainly includes cholesterol synthesis and transfer. Therefore, apoA-I is closely related to hyperlipidemia and atherosclerosis. Clinically, serum ApoA-I/ApoB has been used as one of the indexes of hyperlipidemia. This article reviewed the study progress in ApoA-I's gene polymorphism and its relationship with reverse cholesterol transport (RCT).

OBJECTIVE: To investigate the effect of Salvia Miltiorrhiza injection (SMI) on peritoneal dialysis solution (PDS) induced injuries of peritoneal structure and function in a rat model, and to observe the relationship between the failure of peritoneal dialysis and expressions of aquaporin-1 (AQP-1) and zonula occluden-1 (ZO-1) in peritoneal tissues. METHODS: Fifty-six SD rats were randomly divided into normal control group, 1.5% PDS group, 4.25% PDS group, 1.5%PDS+1% SMI group, 1.5%PDS+2% SMI group, 4.25% PDS+1% SMI group and 4.25% PDS+2% SMI group. Two-hour peritoneal dialysis test was performed in rats in different groups by intraperitoneal injection for 8-week. Then rats were killed on the 8th week, and the bloods and peritoneal tissues were gathered. The rate of ultrafiltration, clearance rates of urea nitrogen, creatinine and glucose of peritoneum and content of total protein in PDS were detected. Peritoneal membrane histology was evaluated by light microscopy and transmission electron microscopy. Expressions of ZO-1 and AQP-1 proteins in peritoneal tissues were detected by immunohistochemical method, and AQP-1 protein expression was also detected by Western blotting technique. RESULTS: Compared with normal control group, using of 1.5% PDS and 4.25% PDS caused the changes of structure and function in peritoneum, such as pathological change of peritoneum, decreasing of the rate of ultrafiltration (P<0.05), clearance rates of creatinine and glucose (P<0.01) and the expression of ZO-1 protein (P<0.05), and increasing of the expression of AQP-1 protein (P<0.05). Compared with the simple PDS groups, the pathological damage of peritoneum was lessened and the rate of ultrafiltration and clearance rates of creatinine and glucose were increased in the 1.5% PDS+2% SMI group and 1.5% PDS+2% SMI group. Expression of AQP-1 protein was decreased by 1.5% PDS+2% SMI as compared with 1.5% PDS (P<0.05).[JP] CONCLUSION: SMI can relieve the injuries of function and structure of peritoneum by down-regulating the expression of AQP-1 protein.

Objective To investigate the association between the expression of heparanase(Hpa) and the invasion of choriocarcinoma by studying the expression of Hpa in human choriocarcinoma cell lines JEG-3 and JAR and human chorionic villous tissues.Methods(1)Matrigcl invasion assays were used to detect in vitro invasive ability of JEG-3 cells and JAR cells.(2)Expression of Hpa protein in the human chorionic villous tissues and choriocarcinoma cell lines(JEG-3 cells and JAR cells)were detected by immunocytochemistry and western blot.Results(1)The invasive cell number was significantly larger in JEG-3 cells than in JAR cells(191?17 vs 106?13,P