OBJECTIVE To investigate drug-resistance status of Klebsiella pneumoniae in county hospital and provide the reference for rational application of antibiotics.METHODS Drug-resistance was analyzed by K-B methods.The ESBLs producing strains were detected by disc confirmed test.RESULTS The detection rate of ESBLs producing K.pneumoniae was 34.7% in our hospital.Among 216 strains of K.pneumoniae,the susceptibility rates to imipenem and meropenem were all 100.0%.And the resistant rate to amikacin was 25.0%.The resistant rates to the other 9 antibiotics were all over 30.0%.CONCLUSIONS Drug-resistance status of K.pneumoniae is very serious in county hospital.We should think highly of monitoring and controlling drug-resistance of clinical strains in order to prevent breaking out and surveillance.

OBJECTIVE To study the infection of Pseudomonas aeruginosa after brain operation and drug-sensitivity.METHODS The system of BBLCrystalTM MIND-Crystal identification and Chinese software were used to identify P.aeruginosa.Drug-sensitivity was performed by K-B methods in accordance with NCCLS.RESULTS Totally 261 specimens were isolated from 126 patients during Mar to Dec 2005.From them 99 strains of P.aeruginosa were detected from specimens of 38 patients.There were 50 strains from phlegm,20 strains from suction in trachea,6 strains from urethral catheterization,8 strains from vein catheter,and 15 strains from wound excretion.Seventy seven strains(77.7%)were isolated during the peak period of May to August.Strains that were isolated in the same period possessed uniform drug-sensitivity results,which hinted epidemic of the same clone of P.aeruginosa in the Deparment of Cerebral Surgery.Drug-sensitivity results showed P.aeruginosa had been resistant to the common antibiotics.CONCLUSIONS Infection with P.aeruginosa after brain operation and drug resistance have already been serious,so we should think seriously of it.

Objective To study the role of 1,4,5-trisphosphate inositol(IP3) and bax gene expression in inhibiting transplanted hepatocellular carcinoma of nude mice by quercetin.Methods After animals with hepatocellular carcinoma were treated with quercetin 1mg/kg/d(ip)for 3 weeks,the volume and weight of tumor was measured,and IP3,Bax mRNA,and Bax protein were assayed by IP3- Birtrak assay,RT-PCR,and Western blotting,respectively;and compard with coutrol group.Results The tumor volume and weight of animals treated with quercetin were lower than those of control[(15.8?10.1) mm3 vs.(52.3?26.5 mm3;(44.8?10.4) mg vs.(91.3?31.4) mg],IP3 content was lower than that of control[(15.9?2.8)pmol/mg protein vs(35.3?6.6)pmol/mg protein],Bax mRNA expression was not significantly different between the 2 groups[RI which was the gray degree multiply area of bax / the gray degree multiply area of ?-actin(0.64?0.12) vs.(0.56?0.15)],Bax mRNA expression was higher in group treated with quercetin than that of control[(3.16?0.95) vs.(1.37?0.48)].Conclusions Quercetin can inhibit growth of transplanted hepatocellular carcinoma of nude mice by reducing IP3 production and bax protein expression.

Objective To study the effect of licorice on the pharmacokinetics of lovastatin in hyperlipidemic rat model.Methods Eighteen rats were randomly divided into control group (n =6) and test group (n =12).Rats in the test group were administered high fat diet to construct hyperlipidemic rat model.The 12 hyperlipidemic rats were then randomly divided into two groups:lovastatin group (n =6) and lovastatin combined with hcorice group (n =6).The rats in both groups were administered lovastatin capsule (20 mg/kg,0.5％ CMC-Na solution) after receiving licorice (for lovastatin combined with licorice group) or saline (for lovastatin group) for 7 days.Blood samples were collected at different time points before and after the administration of lovastatin capsule.The plasma concentrations of lovastatin and lovastatin acid (an active metabolite of lovastatin) were determined by LC-MS/MS method.Pharmacokinetics parameters were calculated using DAS 2.0 software,and the two groups were compared using SPSS 18.0 software.Results Long-term administration of licorice resulted in a significant increase in the plasma level of lovastatin acid in the hyperlipidemic rat,and the corresponding mean Cmax was approximately 80％ higher than that of the lovastatin group (P ＜ 0.05),while AUC0-t and AUC0-t increased by 115％ and 109％,respectively (P =0.005 and P =0.027).Cmax and AUC of lovastatin also increased,but there was no statistical significance (P ＞ 0.05).Conclusion Licorice can inhibit the metabolism of lovastatin in hyperhpidemic rats and increase its exposure in vivo.

Objective To explore the role of estradiol decline in the early luteal phase (the 2nd day after oocyte retrieval) in the prediction of in-vitro fertilization outcomes. Methods A total of 236 cases under in vitro fertilization-embryo transfer (IVF-ET)/ intracytoplasmic sperm injection-embryo transfer(ICSI-ET) cycles were included in this retrospective study of their medical records. The cases were divided into three groups according to the levels of estradiol decline on the 2nd day following OPU: group A (n = 70) with the decline rate of less than 70%, group B (n=114) with the decline rate of 70%~80%and group C (n=52) at the decline rate of more than 80%. The comparisons were done among the three groups in terms of the number of oocyte retrieval, the rate of fertilization, the rate of best oocytes and the rate of miscarriage. Results There were no significant differences statistically in the number of oocyte retrieval, the rate of fertilization, the rate of best oocytes and the rate of miscarriage (P > 0.05). But the rates of clinical pregnancy rate in groups A and B were significantly higher than that in group C (68.6% and 68.4% vs. 44.2%) (P < 0.05). Conclusion The levels of estradiol declines in the early luteal phase may be important in the prediction of IVF outcomes.

AIM:To study the HPLC fingerprints and to establish a sensitive and specific method for controlling the quality of Herba Ephedrae. METHODS: HPLC method was set up,using CLCphenyl column(5 ?m 4.6 mm?150 mm) with gradient elution,the mobile phase was comprised of acetonitrile and phosphoric acid(0.1%),the UV detection wavelength was at 215 nm,with a flow rate of 1.0 mL/min. RESULTS: There were 17 common peaks in the fingerprints of Herba Ephedrae based on 10 habitats.Sum of peak areas was larger than 90% total area.The similarity was compared and the HPLC fingerprints was set up. CONCLUSION: The quality of Herba Ephedrae can be controlled effectively by HPLC fingerprints.

AIM: To develop a new identification of crude drug Herba Ephedrae. METHODS: Using the method of X-ray diffraction fourier fingerprint patterns for the analysis and calculation of 12 habitats of Herba Ephedrae. RESULTS: The standard X-ray diffraction fourier fingerprint pattern of Herba Ephedrae was obtained with 26 peaks. CONCLUSION: The identification and quality control of Herba Ephedrae can be accomplished with X-ray diffraction fourier fingerprint patterns analysis.

Objective To improve standards of diagnosis and therapy for acute focal bacterial nephritis bY comparing the characters of acute focal bacterial nephritis and acute pylonephritis.Methods Thirty-five patients of upper urinary tract infection whoever accepted ultrasonographic and computed tomographic (CT) examinations in Beijing Hospital from January 2007 to January 2013 were studied retrospectively.Eighteen patients were diagnosed as acute focal bacterial nephritis (AFBN) according to CT imaging features,the other 17 patients were diagnosed as acute pyelonephritis (non-AFBN).The clinical feature,laboratory and imaging examination were compared between the two groups.Results Leukocyte count,percentage of neutrophil,fasting blood-glucose,ESR,NAG/Cre of urine,the combination of more than one kind of antibiotics and duration of antibiotic treatment were higher or much more in AFBN group patients than non AFBN patients,the differences between two groups were statistical significance(P ＜ 0.05).Urine microorganism culture was mainly Escherichia coli no matter in AFBN group or non-AFBN group,microorganism species of AFBN patients were more complex than non-AFBN patients.The most common contrast-enhanced CT feature of AFBN included local distribution of inflammatory area,wedge-shaped or unregular-shaped hypodense lesions on bilateral or single kidney,and mass-like hypodense lesions in more severe cases.NAG/CRE of urine was higher in AFBN group patients with bilateral kidney lesions than with single kidney lesions and non AFBN patients.The remission time of AFBN and non-AFBN was (3.4 ± 2.9) d,(3.0 ± 1.8)d respectively.The relapsing rate of AFBN and non-AFBN within half a year was 2/18,2/17,respectively.Conclusions The symptoms of acute focal bacterial nephritis are not specific.Abdominal contrast-Enhanced CT,ESR,leukocyte count,urine NAG/CRE are probably a predictor of AFBN.Antibiotic treatment with appropriate usage and sufficient duration could bring satisfactory outcomes and prognosis in acute focal bacterial nephritis and pyelonephritis patients.

Objective To evaluate short-term curative effect of chloroquine combined with traditional Chinese medicine in treatment of erosion lichen planus. Methods 40 cases were chosen and randomly divided into two groups. The treatment group included 20 cases were treated with chloroquine combined with traditional Chinese medicine. The control group with 20 cases was treated only with chloroquine. The curative effect of the two groups were compared. Results Comparing the two groups, in the treatment group, the pain was remarkably relieved and the erosion areas were obviously smaller. Significant changes were observed statisticly. The overall effectiveness of the treatment group was 85% ,but that of the control group was 65%. Conclusion The treatment of chloroquine combined with traditional Chinese medicine have a short-term clinical effect on erosion lichen planus.

AIM:To investigate the role of 1,4,5-trisphosphate inositol(IP3)and Fas gene expression in apoptosis of HepG2 cells induced by quercetin.METHODS:HepG2 cells were treated with quercetin at different concentrations(including 20,40,60,80 ?mol/L)for 72 h and treated with 60 ?mol/L quercetin for 6 h,12 h,24 h,48 h and 72 h.IP3,Fas mRNA,Fas protein and apoptosis rate were assayed by IP3-3H Birtrak assay,RT-PCR,Western blotting and flow cytometry,respectively.RESULTS:When HepG2 cells were incubated with different concentrations of quercetin for 72 h,the IP3 content was lower than those in control.Fas mRNA expression,Fas protein expression and the apoptosis rate were higher than those in control.When HepG2 cells were incubated with quercetin for 6 h,12 h,24 h,48 h,72 h,the IP3 contents were lower than those in control incubated with 60 ?mol/L quercetin for 12 h.Fas mRNA expression was higher than that in control incubated with 60 ?mol/L quercetin for 12 h.Fas protein expression was higher than that in control.The apoptosis rate was significantly higher than that in control incubated with 60 ?mol/L quercetin for 24 h(P