Kawasaki disease (KD) is one of the most common causes of acquired heart disase in children,the development of which is closely related to the allergic diseases.Recent studies have manifested that the incidence of allergic diseases in children with KD is far higher than that in those children without KD.Meanwhile,the morbidity of KD in allergic children is significantly higher than that in those who do not have family medical history of allergic diseases.By far,the mechanisms causing interaction between KD and allergic diseases still remain unclear.Possible mechanisms include IgE-meditated immune response,release of large quantity of cytokines and inflammatory meditators as well as atopic family history.

Kawasaki disease(KD) is the most common reason for acquired heart disease in children,which is closely related to autoimmune response and the release of cytokines in vascular endothelial cells.Steroids are strongly effective in countering inflammation as well as immune response.In the time of being,it has become a hot spot that steroids play an important role in the initial treatment for acute KD and for incomplete and refractory KD.

Objective To investigate whether efflux mechanism is involved in azole resistant Candida albicans strains isolated in China. Methods We compared rhodamine 6G uptake and glucose induced efflux between azole sensitive and azole resistant strains to elucidate resistance to azole due to efflux pump, then measured mRNA level of efflux gene cdr1 and cdr2 by reverse transcriptase polymerase chain reaction (RT PCR). Results Rhodamine 6G moved from extra into the intracellular compartment in both azole resistant and azole sensitive strains quickly when in glucose free incubation condition. However, efflux of rhodanmine 6G was enhanced significantly in azole resistant strains and decreased in azole sensitive strains while adding glucose in the media. The mRNA level of efflux gene cdr1 and cdr2 was higher in clinical azole resistant strains and fluconazole induced resistant strains than strains reverted from resistance to sensitivity. However, all such strains had higher efflux gene expression than azole sensitive strains. Conclusions Efflux mechanism is associated with the resistance to azole in Candida albicans strains isolated in China. Fluconazole can induce efflux pumps expression leading to resistance to azole but the resistance to azoles can be reversed after withdrawing fluconazole.

Over a 2 year period,53 transbrachial approachs were selectively performed in 42 cases.5～6F catheters were insertend directly over a guide-wire vie a puncture site 1～3cm over the antecubital fossa of left arm,where the artery would be less mobile.Selective cathetenization of the hepatic artery,superior mesenterie artery,infradiaphragmatic artery,renal artery,internalilliac artery,femoral artery and their branches were suessesfully achieved.No significant complications were encountered.This method proved to be a safe,reliable and well-tolerated ahernetive,though the exemination technique was more in demanding.The indications,techniques and complications of the method were discussed.

The multiplex gene expression genetic analysis system is a novel analytical platform,characterized by its primer design with gene-specific and universal sequences.The fluorescence capillary electrophoretic separation is used to compare and analyze the expression levels of multiplex PCR products.This technology is simple,rapid,productive and cost-effective,and holds great promise for cancer classification and biomarkers discovery.

Objective To investigate the expressions of tenascin(TN)and microvessel density(MVD) in esophageal squamors cell carcinoma.Methods The experessions of TN and MVD were observed by immunohistochemiccal methods in 91 cases of esophageal carcinoma and 47 cases of ono-esophageal carcinoma.Results ①The expression of tenascin in esophageal cancer was much high than that in t he normal tissue(t=12.331,P＜0.01).②Over expression of TN was related to th length(F=12.373,P＜0.01),the invasion(F=11.039,P＜0.01),the lymphatic metastasis(F=6.882,P=0.01)and the pathologic trade(F=5.060,P=0.003)of cancer.③The expression fo MVD in the esophageal carcinoma tissues was stonger than that in the nonesophageal carcinoma tissues(t=6.023,P＜0.01).④Over expression of NVD wsa related to the length(F=9.033,P＜0.01),the lymphatic metastasis(F=12.429,P＜0.01)and the pathologic grade(F=5.717,P＜0.01)of cancer.⑤The positive expressions of TN and MVD were associated(P=0.001).Conclusion TN;s voerexpressions may be a biological marker in esophageal squamous cell carcinoma..It was associated with the expression of MVD.The levels of TN and NVD were useful molecular markers for evaluating malignancy degree and lymph node metastasis of esopageal carcinoma.

Neurological complications of viral hepatitis mainly include hepatic encephalopathy,hepatic myelopathy,peripheral neuropathy,meningoencephalitis,and epilepsy.Hepatic encephalopathy is a common complication with mature diagnosis and treatment methods,and the other complications are relatively uncommon and lack treatment methods.This article introduces the classification,clinical manifestation,and treatment of each complication,in order to find the optimal therapies for neurological complications of viral hepatitis.

With the informatization of modern hospital,the management model & structure for medical consumables evolves to adapt to the requirements of hospital.Then,some suggestions are put forward based on the analyses of the problems of hospital consumables management and the advantages of direct distribution.

MicroRNA could participate in a variety of physiological and pathological processes via iden-tifying the target genes by their seed sequences and regulating gene expression at post-transcriptional level. Glo-merular mesangial cell is one of the most chief cells of the glomerulus and plays an important role in the renal fi-brosis. The mesangial cellular proliferation and dysfunction may appear once attacked by pathogenic factors. Then,the extracellular matrix increases in the stimulation of inflammatory cytokines,eventually,resulting in glo-merular sclerosis and renal fibrosis. Reports have shown that series of microRNA could take part in the process of injury of mesangial cells and lead to renal fibrosis. MicroRNA could be a potential therapeutic target for early chronic kidney diseases.

Objective To investigate the impacts of health education on awareness of hyperglycemia,self-efficacy and serum level of blood glucose in patients with coronary heart disease (CHD).Methods Two hundred CHD patients were enrolled in this study and assigned to the normal blood glucose (NGT) group ( n =60),impaired glucose regulation (IGR) group ( n =50),and type 2 diabetes mellitus (T2DM) group (n =90).Health education programme was carried out before and after discharge from the hospital.Fasting plasma glucose (FPG),2 h postprandial blood glucose (2 hPG) and glycosylated hemoglobin ( HbA1c ) of each group were assessed at baseline and 3 or 6 months after the intervention.Chisquare or t test was used for data analysis.Results After the intervention,the awareness of hyperglycemia was significantly improved in each of three groups (NGT group:x2 values were 17.207,23.582,37.046,39.046,38.686,46.218,28.643,39.895,37.046 and 33.333,respectively,all P ＜ 0.05 ; IGR group:x2values were 31.723,23.529,23.389,27.542,21.091,27.307,20.219,38.235,24.669 and 26.475,respectively,all P ＜0.05; T2DM group:x2 values were 21.161,20.680,14.612,23.104,22.383,29.436,19.444,32.526,24.671 and 31.696,respectively,all P ＜ 0.05 ).Self-efficacy scores of symptom selfmanagement and common disease management were increased after the health education intervention (t values were 12.75 and 7.21,respectively,both P ＜ 0.05 ).At 3 or 6 months,the NGT group showed on significant change in FPG,2 h PG and HbAlc (F values were 2.35,1.97 and 2.01,respectively,all P ＞0.05) ; however,FPG,2 h PG and HbAlc of the IGR and T2DM group were significantly decreased ( IGRgroup:F values were 8.07,8.89,and 10.23,respectively,all P ＜ 0.05 ; T2DM group:F values were 12.35,11.56 and 8.99,respectively,all P ＜ 0.05 ).Conclusion Health education could improve the awareness of hyperglycemia,self-efficacy and glucose metabolism in patients with CHD.