alyzing allergen of Alternaria alternata.

Objective: To investigate the effects of surface sealing on the shear bond strength(SBS) of the brackets. Methods: Twenty extracted premolars for orthodontics were cut into the buccofacial teeth pieces and etched by Transbond~TM Plus self-etching primers for 30 seconds firstly, then divided into two groups with 10 teeth pieces in each group randomly. ① experiment group, the buccal surface of the enamel was sealed with Filtek Flow 1400A3 flowable composite resin before bracket bonding;②control group, the bracket was bonded to the buccal surface of the enamel directly. All specimens were stored in deionized water at 37 ℃ for 48 h, thermocycled for 1 000 times at 30-second intervals between 5 ℃ and 55 ℃ water baths, and debonded with a universal testing machine. The amounts of resin remaining on the teeth pieces after debonding by using the resin remnant index (RRI), which were examined under 10×magnification stereo-microscope. Results: The SBS(MPa) between experiment group and control group was 8.08±1.38, 8.34±1.37 respectively, whereas the RRI was 2.80±1.03, 3.20±1.03 respectively. The SBS, RRI between experimental group and control group revealed no statistically significant differences. Conclusion: Surface sealing shows adequate bond strength, which is considered a clinically acceptable bond strength. The debonding of bracket is not affected by the surface sealing.

The aim of this study is to investigate the apoptotic inhibition and its molecular mechanism of dexamethasone (DEX) acting on cisplatin (CDDP)-induced apoptosis of human lung adenocarcinoma cell SPC-A1; SPC-A1 cells were pre-cultured in vitro for 24 hours with DEX in different concentrations and then CDDP was added in different concentrations for culturing for further 48 hours. The survival rates of the cells were determined by MTT. The expression of serum/glucocorticoid-induced kinase (SGK-1) and mitogen-activated protein kinase phosphatase-1 (MKP-1) in SPC-A1 cells after being cultured by 1 micromol/l DEX at different time was detected by semi-quantitative RT-PCR technology. The expression of glucocorticoid receptor (GR) in SPC-A1 cells was measured by immunohistochemistry (IHC) with biotin-labeled anti-GR. The results of MTT showed that SPC-A1 cells had resistance to CDDP-induced apoptosis with pre-cultured DEX and the resistance intensity presented DEX concentration-dependent. The expressing quantity of SGK-1 in SPC-A1 cells stimulated by DEX could be elevated and increased with intention of time, but the express of MKP-1 was not detected. Up-regulated expression of GR in SPC-A1 cells stimulated by DEX was detected by IHC. The number of cells expressing GR in SPC-A1 cells was significantly higher than that in the control group. The results showed that DEX inhibited apoptosis of SPC-A1 cells induced by CDDP. The possible molecular mechanism is that elevated expression of GR induced by DEX up-regulates the expression of SGK-1 which locates at the downstream of anti-apoptosis pathway. The apoptosis resistance of SPC-A1 cells may account for all above the factors.
Adenocarcinoma ; pathology ; Apoptosis ; drug effects ; Cell Line, Tumor ; Cisplatin ; pharmacology ; Dexamethasone ; pharmacology ; Dual Specificity Phosphatase 1 ; metabolism ; Humans ; Immediate-Early Proteins ; metabolism ; Lung Neoplasms ; pathology ; Protein-Serine-Threonine Kinases ; metabolism ; Receptors, Glucocorticoid ; metabolism ; Up-Regulation

The etiology of pulmonary fibrosis is complex. The occurance and development of this disease involve many cell signal transduction pathways, including MAPK signaling pathway, JAK-STAT signaling pathway, Smad signaling pathway, PI3K-Akt signaling pathway, NF-κB signaling pathway and so on. Numbers of experiments have shown that intervention in signal transduction pathways with TCM can delay the progression of fibrosis, which provides good therapeutic methods for this disease. This article reviewed study on TCM intervention in different signal transduction pathways in the pulmonary fibrosis in China.

Objective To investigate the effect of ethyl pyruvate (EP) on the expression of caspase-3 in the hippocampus and the learn-ing and memory ability in neonatal rats with hypoxic ischemic brain damage (HIBD). Methods 90 Wistar rats of 7 days old were randomly divided into sham operation group (n=15), HIBD group (n=15), EP1 group (n=15, 10 ml/kg), EP2 group (n=15, 30 mg/kg), EP3 group (n=15, 50 mg/kg) and EP4 group (n=15, 100 mg/kg). The model was established with Rice's method. 30 minutes before operation, and every 24 hours after operation, EP groups were injected with 10 ml/kg, 30 mg/kg, 50 mg/kg, 100 mg/kg EP in abdomen respectively, for 2 weeks. Af-ter treatment, the caspase-3 positive cells were observed by immunohistochemical staining, and the latency and the times crossing the target quadrant were tested by Morris water maze test. Results The caspase-3 positive cells were less in EP groups than in HIBD group (P<0.05), expect EP1 group (P>0.05), especially in EP3 group (P<0.01). The latency and the times crossing the target quadrant were better in EP groups than in HIBD group (P<0.05), expect EP1 group (P>0.05), especially in EP3 group (P<0.01). Conclusion Ethyl pyruvate can de-crease the expression of caspase-3 in hippocampus, and improve the ability of memory and learning in neonatal rats with HIBD.

Objective The study was focused on the current situation of human resources of pharmaceutical enterprises in Heilongjiang Province, to solve the problems of workforce shortage in small- and medium-sized pharmaceutical enterprises, and put forward corresponding countermeasures. Methods A convenient sampling method was used to investigate the basic situation of 12 pharmaceutical enterprises in Heilongjiang Province in June - November 2016, and interviews and questionnaire survey were conducted with the employees of human resources departments. The basic situation of the survey included: the general situation, the demand for talent, the development of pharmaceutical enterprises and so on. Staff interviews included: the number of talents introduced and the number of outflows in the past 3 years, human resources needs, knowledge and ability of pharmaceutical enterprises. The staff survey included: employee job satisfaction, turnover tendency and career development prospects. Results The number of employees in the 12 pharmaceutical enterprises was 22065. Employees' age was in 34 - < 44 years old, with low education qualifications, 74.6% (16456/22065) of employees attended junior college or below . There were 6246 technical personnel , most of them (3678) received college education. In the interviews with 36 employees, it was found that the pharmaceutical enterprises were short of workforce. The unstable sources for the workforce and brain drain were the biggest problem in the development of pharmaceutical enterprises. In the 591 questionnaires, 24.6%(145/591) of employees had the idea of changing jobs in the past year. Conclusion Pharmaceutical enterprises should pay attention to the introduction and training of talented staff, and develop a series of preferential conditions to attract them, so as to achieve the goal of thriving enterprises by talented workforce.

The pilot work urban workers medical network settlement of Anhui province has launched in full scale,effectively facilitating the population seeking medical service elsewhere.In this regard,the medical settlement of a pilot hospital is cited as an example to introduce the networked medical settlement in remote places,summarizing problems encountered,and coming up with proposals accordingly.

BACKGROUND: Na+/dicarboxylate transporter protein (NaDC3) participates in the metabolism of energy, which may has effect on cellular senescence; however, the mechanism is not clear. OBJECTIVE: To investigate the role of human NaDC3 in cellular senescence by means of cell cycle and P16 measurements. DESIGN, TIME AND SETTING: The single sample observation was performed in the Nanjing Drum Tower Hospital from December 2007 to May 2008. MATERIALS: The WI-38 cell was purchased from ATCC Company, the retrovirus vector pLNCX2, sense vector PLNCX2-hNaDC3 and antisense vector PLNCX2-AshNaDC3 was provided by Clontech Company. METHODS: hNaDC3 was introduced into WI-38 cells with retroviral virus-mediated transfection. The integration and expression of exotic gene were confirmed. Whereafter, to examine the effects of hNaDC3 on cellular senescence of WI-38 cells by cell cycle and expression of P16. MAIN OUTCOME MEASURES: The integration and expression of hNaDC3, the cell morphology and generation of WI-38, the cell cycle and expression of P16 were observed. RESULTS: The results of Northern blot and Western blot revealed that hNaDC3 were expressed in WI-38/ hNaDC3 cells at gene and protein level, while, in WI-38/As hNaDC3 cells, there were integration of hNaDC3 mRNA with not protein expression. Compared with the control cells, WI-38 cells transfect with hNaDC3 cDNA showed cell generation decreased by eight to twelve passages, which in a low cell growth rate with cell cycle arrested at G1 phase, and the expression of P16 was elevated. CONCLUSION: Human NaDC3 gene may contribute to the process of cellular senescence of human diploid fibroblasts by increasing expression of P16.

Objective To study the relationship between cognitive dysfunction and depression and event-related potential(ERP) in patients with silent brain infarction(SBI).Methods 68 SBI patients were tested by the Hamilton Depression Rating Scale for Depression(HAMD),scale of mini mental state examination(MMSE)and ERP;and the results were compared with those in 55 patients without cerebral infarction(control group).Results In the SBI group,the HAMD score was ≥17 in 31(45.6%)cases(depression subgroup)and

The necessity of the Electrocardiograph anti-interference performance test are introduced,and the principle and method of anti-interference performance test based on standard YY 1139-2000 are also provided.Through the experiment of anti-interference,the reliability and practicality of our self-made testing device is confirmed.