Acupuncture Therapy ; Adult ; Hoarseness ; therapy ; Humans ; Hysteria ; therapy ; Male

Aim To investigate the difference in pharmacokinetics between gatifloxacin mesylate(GATM) and gatifloxacin(GAT) in SD rats in vivo.Method The plasma concentration-time courses of GAT in rats were measured by HPLC method after a single oral dose of GATM and GAT,and the pharmacokinetic parameters were estimated by program 3p97 software.Results The results shown that both of GATM and GAT plasma concentration-time courses were best fitted to two-compartment models after a single oral dose(GATM 23.43 mg?kg~(-1)and GAT 20 mg?kg~(-1),respectively).The major average pharmacokinetic parameters of GATM were as follwing: T_(2)?: 9.13 h,AUC: 15.05 mg?L~(-1)?h,C_(max): 4.41 mg?L~(-1),and T_(max): 0.5 h,respectively.On the other hand,the pharmacokinetic parameters of GAT were as follwing: T_(2)?:10.70 h,AUC:13.84 mg?L~(-1)?h,C_(max):4.25 mg?L~(-1),T_(max):0.5 h,respectively.Conclusion Under the equi-mole dose condition treated as above,both of pharmacokinetic characters between GATM and GAT were not significantly differences in rats

Objective To explore epicanthus correction by transverse incision method and concurrent double-eyelid incision operation.Methods The transverse straight-line incision was performed in new inner canthus to the original canthal corner point; after the original inner canthus corner point was reached,the oblique-line parallel incision was performed along the lower eyelid so that full subcutaneous separation was obtained on the upper and lower incision; the malpositioned isomerous orbicular muscle and thickened tissue were released and excised so that the epicanthus skin was naturally restored; the superficial head of the inner canthus ligament was folded and sutured when necessary ; after small cat ears at the temporal side of the inner canthal incision was removed,routine double eyelid plasty was then performed,but the double eyelid and the inner canthus incisions were not continuous.Results The procedures were clinically applied in 258 cases and followed up for 3-18 months,showing that the epicanthus has disappeared,new inner canthus was in good appearance,the scar was not obvious,recurrence of the epicanthus was not found and double eyelids were beautiful in appearance.Conclusions The epicanthus correction by transverse incision and concurrent double-eyelid plasty is a simple and ideal approach for correction of single-edged eyelid with epicanthus and it is worthy of clinical promotion.

OBJECTIVE: In recent years, in the field of neurosurgery, people have tried to study the mesenchymal stem cells(MSCs) transplantation for treatment of spinal cord injury and review its related studies.DATA SOURCES: We did a computer-search of Medline for spinal cord injury related articles published in English from January 1990 to November 2004, with the key words of "mesenchymal stem cells, transplantation,spinal cord injury". Meanwhile, We also searched Chinese Biomedical Literature Database (CBM) for bone marrow-derived mesenchymal stem cells for transplantation of spinal cord injury related articles published from January 1990 to November 2004, with the key words of "mesenchymal stem cells, transplantation, spinal cord injury" in Chinese.STUDY SELECTION: All articles were selected firstly, and those relevant to progression of mesenchymal stem cells transplantation in spinal cord injury therapy were collected. The full texts of the rest literatures were looked up and the latest evolutional articles were enrolled.DATA EXTRACTION: A total of 32 articles about the mesenchymal stem cells transplantation in spinal cord injury therapy were collected. After the exclusion of repetitive or similar studies, 19 articles met our criteria.DATA SYNTHESIS: Aiming at the spinal cord injury therapy by means of mesenchymal stem cells transplantation, the viewpoints of collected articles were summarized. We found bone marrow derived mesenchymal stem cells could supply lesioned part as filler. Directional regeneration accomplishes the construction of the function that transfers up and down for the nerve cell anchor through surrounding structure. The microenvironment which can inhibit the regeneration of the glial cell, protect the survival of nerve cell body, promote the regeneration of autogenic nerve cells shall be created in transplant. The international successful experience was introduced emphatically, and the most hopeful outlook was suggested.CONCLUSION: The transplanted MSCs survive, migrate to the injury site and differentiate into neurons and astrocytes. Transplantation of MSCs following spinal cord injury may improve the recovery of spinal function and may be an effect way in treating spinal cord injury.

OBJECTIVE:To investigate the stability of injectable pantoprazole sodium in 4 kinds of infusions.METHODS: A HPLC method was adopted in this determination, the content change of injectable pantoprazole sodium was determined within 4 hours' mixing with 4 kinds of infusions and its change of color was observed; meanwhile the effects of pH value, potassium,magnesium,calcium ions on the stability of injectable pantoprazole sodium were investigated.RESULTS:No obvious changes were noted in terms of contents, appearances, pH value, UV - absorption maximum wavelength for the injectable pantoprazole sodium solution within 4 hours either in 4 kinds of common infusions or in potassium,magnesium,calcium ions water solutions.It was extremely unstable when pH value of pantoprazole sodium solution was lower than 7.0;when its pH value was 7.0, its color became yellowish yet without obvious loss of content; it is stable within 4h when its pH value was above 8.0.CONCLUSIONS:Injectable pantoprazole sodium remains stable within 4 hours either in 4 kinds of common infusions or in potassium, magnesium, calcium ions water solutions, the pH value has a great influence on the stability of pantoprazole sodium solution.

Objective To study the inhibition of neutrophil apoptosis induced by interleukin-6 (IL-6) and evaluate the effect of propofol on IL-6 induced inhibition of neutrophils apoptosis. Methods Neutrophils were isolated from 20 healthy volunteers( 20-55 yr) and incubated in RPMI1640 cell culture medium . Neutrophils from each volunteer were divided into 6 parts and incubated with NS (group Ⅰ -control), IL-6(group Ⅱ), propofol( group Ⅲ), intralipid (the lipid vehicle of propofol-group Ⅳ ), IL-6 + propofol(group Ⅴ), IL-6 + intralipid(group Ⅵ ) respectively. After 24 h of incubation, neutrophils were stained with acridine orange and ethidium bromide and observed under fluorescence microscope. Apoptosis index was calculated.Results IL-6 inhibited neutrophil apoptosis (IL-6 35.80 ?8.59 vs control 52.00?10.60, P

0.05]. During the (6.5?2.4) month (range 1 to 10 months) follow-up, the angina incidence rate of the Firebird group was lower than the BMS group (21.0%, 35 5%, P

Objective To investigate the effect of rosiglitazone on neointima and inflammatory factor after carotid artery injury of rats. Methods A total of 60 SD rats were divided randomly into 3 groups: the control group, the surgery group and the resiglitazone therapeutic group. The left common carotid arteries were injured by balloon in the surgery group and the therapeutic group. Rosiglitazone was administrated intragastricly to therapeutic group. The plasma tumor necrosis factor-? (TNF-?) and interleukin-6 (IL-6) levels were detected with radioimmunoassay at 4h, 1 and 7d after the operation. After 2 weeks, the injured vessels were stained by HE and immunity histochemistry method,then observed with microscope. Neointimal area (NIA), internal elastic lamina area (IELA), stenosis index (SI) and optical density value of protein expression of NF-?B were calculated. Results Rosiglitazone inhibited the neointima of injured vessels, decreased the protein expression of NF-?B and reduced plasma TNF-? and IL-6 levels. Conclusion Rosiglitazone can inhibit the inflammatory reaction of injured vessels and lighten the restenosis of injured vessels.

Objective To observe the effects of hypoxia preconditioning on the mitochondria ultrastructure of hepatocyte during liver transplantation in rats.Methods A modified orthopotic liver autotransplantation model was used to simulate liver transplantatation.Seventy two Sprague-Dawley rats were randomly divided into the following three groups:the normal control(group NC),the autotransplantation group(group AT),and the hypoxia preconditioning group(group HP),with twenty four rats in each group.Group HP were given an 8% oxygen atmosphere for 90 minutes before the operation.At 1,6,and 24 hours after the operation,the rats were killed and the following tests were conducted:1.The morphology of mitochondria was observed under transmission electron microscopy(TEM);2.Mitochondria were quantitatively analyzed by a MiVnt image analysis system.ResultsHepatic cells in group AT showed typical injury signs under TEM,the appearance of hepatocytes and mitochondria in group HP were much better than the group AT,and the areas,perimeters and diameters of mitochondria in group HP were much smaller than those in group AT by a significantly amount(P

Objective To demonstrate the clinical results of the patients with failed cemented femoral component due to aseptic loosening with extensive porous-coated femoral implants. Methods Be-tween 1998 and 2003, twenty hips (20 patients) with a failed cemented femoral component were revised us-ing extensive porous-coated femoral components and allograft. There were 12 males and 8 females. The mean age of the patients at the time of revision was 64 years (41-77 years). All of the revised femoral com-ponents were cemented stems,with first-generation cement technique in 18 hips and second-generation ce-ment technique in 2 hips. The indications for hip arthroplasty included osteonecrosis of the femoral head in 14 hips and femoral neck fracture in 6 hips. The interval from primary hip arthroplasty to revision surgery ranged from 5 to 17 years, with an average of 11.5 years. There were 4 re-revisions in this group. Based on Paprosky classification for bone deficiency, 3 hips were of type I, 6 of type II and 11 of type IIIA. The com-ponents implanted included AML (DePuy) in 5 cases , Solution (DePuy)in 7, full-coated (Zimmer)in 6 and Perfecta Extend Stems (Wright Med) in 2. The postoperative stability was evaluated according to the Engh criteria. The patients were followed for an average of 40 months (18-60). Results At the latest fol-low-up, the mean Harris score was improved from 40 to 85 points postoperatively. The bony ingrowths were found in 17 hips and the fibrosis stability in 3 on postoperative radiographic evaluation. There were no re-revisions in this group. The bone remodeling was observed in all osteolytic zones. Conclusion In the pres-ence of bone loss in the proximal metaphyseal region of the femur, the extensive porous-coated femoral com-ponents may provide a bypass for the deficient proximal bone. It may further obtain initial optimizing pros-thesis-bone fitting and an axial anti-rotational stability in the relatively normal diaphyseal region of the fe-mur to allow the reliable biological fixation with bony ingrowths.