1.Primary squamous carcinoma of intestine: report of a case.
Chinese Journal of Pathology 2009;38(5):350-351
Carcinoma, Adenosquamous
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pathology
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Carcinoma, Squamous Cell
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Humans
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Jejunal Neoplasms
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metabolism
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pathology
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surgery
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Jejunum
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surgery
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Keratins
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metabolism
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Male
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Middle Aged
2.Loss of melanopsin-containing retinal ganglion cells in a rat glaucoma model.
Huai-zhou WANG ; Qing-jun LU ; Ning-li WANG ; Hong LIU ; Ling ZHANG ; Gui-lin ZHAN
Chinese Medical Journal 2008;121(11):1015-1019
BACKGROUNDGlaucoma can cause progressive damage to retinal ganglion cells. These cells can be classified as cells projecting to the superior colliculus and melanopsin-containing retinal ganglion cells, which project to the suprachiasmatic nucleus. This study was to investigate the effects of chronic intraocular pressure elevation on melanopsin-containing retinal ganglion cells in rats.
METHODSChronic intraocular pressure elevation was induced in one eye of adult Wistar rats by cauterization of three episcleral veins. Intraocular pressure was measured at different intervals with a rebound tonometer. Superior collicular retinal ganglion cells were retrogradely labeled from the superior colliculus with Fluorogold. Melanopsin-containing retinal ganglion cells were visualized by free-floating immunohistochemistry on whole-mount retinas. The number of labeled superior collicular and melanopsin-containing retinal ganglion cells were counted in the sample areas on flat-mounted retinas.
RESULTSCompared with contralateral control eyes, the numbers of both superior collicular and melanopsin-containing retinal ganglion cells were significantly reduced after 12 weeks of experimental intraocular pressure elevation ((2317.41 +/- 29.96)/mm(2) vs (1815.82 +/- 24.25)/mm(2); (26.20 +/- 2.10)/mm(2) vs (20.62 +/- 1.52)/mm(2), respectively). The extent of cell loss of the two types of retinal ganglion cells was similar. However, no morphologic changes were found in melanopsin-containing retinal ganglion cells.
CONCLUSIONBoth melanopsin-containing and superior collicular retinal ganglion cells were damaged by chronic ocular hypertension, indicating that glaucomatous neural degeneration involves the non-image-forming visual pathway.
Animals ; Disease Models, Animal ; Glaucoma ; pathology ; Intraocular Pressure ; Male ; Rats ; Rats, Wistar ; Retinal Ganglion Cells ; pathology ; Rod Opsins ; analysis
3.Influence of pitavastatin on vascular endothelial function in young and middle-aged patients with hyperlipidemia complicated carotid plaques
Xiao-Na ZHAN ; Gui-Hua LI ; Ya-Juan WANG ; Li-Juan FU ; Lei GAO
Chinese Journal of cardiovascular Rehabilitation Medicine 2018;27(1):29-32
Objective: To explore therapeutic effect of pitavastatin on young and middle-aged patients with hyperlipidemia complicated carotid plaques and its influence on vascular endothelial function. Methods: A total of 126 young and middle-aged patients with hyperlipidemia complicated carotid plaques [carotid intima-media thickness (IMT) > 1. 5mm]were selected, and were randomly divided into no lipid lowering treatment group (n=62) and pitavastatin group (n=64). Flow-mediated dilation of brachial artery (FMD), carotid IMT and blood lipid levels before and 12 months after medication, and incidence rate of adverse events were recorded and compared between two groups. Results: Compare with before treatment and no lipid lowering treatment group after treatment, there was significant rise in FMD [(6. 70±2. 10) %, (6. 60±2. 35) % vs. (8. 90±3. 60) %], and significant reductions in levels of total cholesterol [(6. 05±1. 40) mmol/L, (5. 67±1. 90) mmol/L vs. (4. 05±1. 20) mmol/L], triglyceride [(2. 18± 0. 72) mmol/L, (2. 08±0. 68) mmol/L vs. (1. 77±0. 65) mmol/L]and low density lipoprotein cholesterol [(4. 65±1. 50) mmol/L, (4. 41±1. 36) mmol/L vs. (2. 01±1. 30) mmol/L]in pitavastatin group, P<0. 05 or<0. 01; there were no significant changes in IMT in two groups. No obvious adverse reaction was found in pitavastatin group. Conclusion: Pitavastatin can significantly improve lipid levels and vascular endothelial function in young and middle-aged patients with hyperlipidemia complicated carotid plaques.
4.Cloning and Phylogenetic Analysis of the Ribosomal Protein L8 cDNA of the Weever(Lateolabrax japonicus)
Xiao CONG ; Zhan-Tao SHAO ; Jin-Duo YUAN ; You-Zheng WANG ; Gui-Wen YANG ; Li-Guo AN
Progress in Modern Biomedicine 2008;8(5):858-861
The complete nucleotide sequence of a cDNA clone encoding weever ribosomal protein L8, WeevL8, is described here. The WeevL8 cDNA has 848 nucleotides in full length and encodes a 257 amino-acid protein with a calculated molecular mass of 28.02 kDa. Two conserved domains have been identified in WEEVL8. It was concluded fromsequence alignment that WeevL8 gene was quite conservative, consistent with its role as a house-keeping gene. A phylogenetic analysis made by L8 protein showed the similar phylogenetic relationship to that with 18s rDNA. The high similarity supports the notion that ribosomal protein L8 can also be used as phylogenetic criterion.
5.Effects of clotrimazole on the growth of oral squamous cell carcinoma.
Hui LIU ; Yue-Hong WANG ; Zhan-Gui TANG ; Ou-Sheng LIU ; Jie ZHANG ; Gui-Yuan LI
Chinese Journal of Stomatology 2010;45(9):545-548
OBJECTIVEto investigate the effects of clotrimazole on the growth of oral squamous cell carcinoma (OSCC).
METHODSOSCC-25 cells growing in log phase were treated with various doses of clotrimazole. The concentration of IC(50), cell cycle and cell cycle related protein were examined.
RESULTSthe concentration of clotrimazole for inhibiting OSCC was IC(50) 8.51 µmol/L. Clotrimazole induced cell cycle arrest in the G(0)-G(1) cell cycle phase, with a concomitant decrease of cells in the G(2)-M and S-phase. Furthermore, clotrimazole significantly decreased the levels of cyclin D, cyclin E and CDK-4.
CONCLUSIONSclotrimazole inhibits the growth of OSCC.
Carcinoma, Squamous Cell ; pathology ; Cell Cycle ; Cell Cycle Checkpoints ; Cell Division ; Cell Line, Tumor ; Clotrimazole ; pharmacology ; Cyclin E ; Humans ; Mouth Neoplasms ; pathology ; Oncogene Proteins
6.Inhibition effects of rhubarb ethanol extract on herpes simplex virus infection in vivo.
Zhi-yu WANG ; Bin XU ; Yan-yan SONG ; Gui-ting WANG ; Hong-zhi XU ; Xiao-fan WANG ; Yong-lei XUE ; Zhan-yong WANG ; Xiu-ping YU
Chinese Journal of Experimental and Clinical Virology 2003;17(2):169-173
OBJECTIVETo know the anti-viral effects of rhubarb ethanol extract (REE) on herpes simplex virus(HSV) infection in vivo.
METHODSBALB/c mice inoculated from tail vein with 0.15 ml of HSV (TCID50=10(3)) were injected hypodermically with REE next day. After divided into seven groups, three groups of mice were given different doses of REE respectively and the other groups as controls. Pathological sections from the liver, spleen, kidney were made at different times of postinfection, and their pathological changes were observed under microscope; the virus titers in viscera were assayed by using plaque formation technique and the rhubarb inhibitions to the infection of HSV in vivo?were observed.
RESULTSNo toxic response to mice were observed for REE injected hypodermically; no pathological changes were observed in different therapy groups of spleens. And those in livers and kidneys at medium- and high-dosed groups disappeared quickly. The effect of low-dosed group was equal to that of positive control group, acyclovir(ACV); the results of the titer tests showed that the virus decreased rapidly by using REE, especially in the medium- and high-dosed groups which were much more marked than the low-dosed group; Q test of the data showed that total mean value had statistical significance (F=49.1459, P<0.01); moreover there were statistical significance between therapy groups (ACV, DH1, DH2, DH3) and non-therapy groups (VC) (P<0.01 ) and between DH2, DH3 and DH1 (P<0.01); no statistical significance were found between DH1, DH2 or DH3 and ACV (P>0.05). Results show that as to the effect of decreasing the average of the total titer, rhubarb is as effective as ACV; furthermore, the medium- and high-dosed groups are superior to the low-dosed group.
CONCLUSIONSREE has significant anti-viral effect on HSV in vivo; there will be a wide application foreground of it in clinical usage.
Animals ; Antiviral Agents ; pharmacology ; Drugs, Chinese Herbal ; pharmacology ; Female ; Herpes Simplex ; pathology ; virology ; Herpesvirus 1, Human ; drug effects ; Male ; Mice ; Random Allocation ; Rheum
7.Effects of recombinant human erythropoietin on brain-derived neurotrophic factor expression in different brain regions of aging rats.
Hu-Qing WANG ; Zhen GAO ; Meng-Yi CHEN ; Hai-Qin WU ; Gui-Lian ZHANG ; Shu-Qin ZHAN ; Ning BU ; Jing-Jie LIU ; Yue-Fen ZHAI
Journal of Southern Medical University 2016;37(4):551-554
OBJECTIVETo explore the effect of recombinant human erythropoietin (rhEPO) on expression of brain-derived neurotrophic factor (BDNF) in different brain regions of aging rats.
METHODSForty male SD rats were randomized equally into negative control group, D-galactose group, EPO treatment group, and positive control group. Rat models of subacute aging were established by continuous subcutaneous injection of 5% D-galactose. Immunohistochemical staining was used to analyze the variation of BDNF expressions in different brain regions of the aging rats with different treatments.
RESULTSSignificant brain region-specific differences in BDNF expression were found among the rats in different groups. Compared with those in the negative control group, the numbers of BDNF-positive cells in the hippocampal CA1 region, CA3 region, dentate gyrus (DG) and frontal cortex were all decreased obviously in D-galactose group (P<0.05) but increased in both EPO group and the positive control group (P<0.05) without significant differences between the latter two groups. In the rats in the same group, the number of BDNF-positive cells varied markedly in different brain regions (P<0.05), and the expression level of BDNF was the highest in the frontal cortex followed by the hippocampal CA3 region and the dentate gyrus, and was the lowest in the hippocampal CA1 region.
CONCLUSIONTreatment with rhEPO enhances the expression of BDNF in rat neural cells, suggesting that rhEPO may protect the nervous system from aging by regulating the BDNF pathway.
Aging ; Animals ; Brain-Derived Neurotrophic Factor ; metabolism ; CA1 Region, Hippocampal ; metabolism ; CA3 Region, Hippocampal ; metabolism ; Dentate Gyrus ; metabolism ; Erythropoietin ; pharmacology ; Frontal Lobe ; metabolism ; Galactose ; Humans ; Male ; Neurons ; drug effects ; metabolism ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Recombinant Proteins ; pharmacology
8.Expression change of apoptosis-associated genes after hyperthermia, chemotherapy and radiotherapy in human colon cancer-transplanted nude mice.
Han LIANG ; Hong-Jie ZHAN ; Bao-Gui WANG ; Yuan PAN ; Xi-Shan HAO
Chinese Journal of Gastrointestinal Surgery 2008;11(3):270-275
OBJECTIVETo investigate the expression change of apoptosis-associated genes in human colon cancer cells transplanted into nude mice after hyperthermia, chemotherapy and radiotherapy.
METHODSHuman colon cancer cell line HT29 was transplanted into the hind limbs of nude mice. Under the laboratory-simulated condition of hyperthermia(43 degree centigrade, 60 min), actual radiation doses and MMC doses were calculated in reference to the clinical practice. The mice were divided into 6 groups according to the treatment approaches: hyperthermia (group A), chemotherapy (group B), radiotherapy (group D), thermochemotherapy (group C), thermoradiotherapy (group E) and thermochemoradiotherapy (group F). The mice were sacrificed at different time points and the tumor tissues were taken for further procedures. The morphologic changes of P53, Bcl-2 and Bax expression in membrane, cytoplasm and nucleus of tumor cell after treatment were observed by immunohistochemistry stain (SP method).
RESULTSAll of the six approaches of treatment could down-regulate the expression of P53 and Bcl-2, and up-regulate the expression of Bax in different levels. There was no significant difference in the amount of reduction of P53 expression among group A, C and E. The extent of reduction in the above mentioned groups was significantly different as compared to group B and D. By comparing to group D, the extent of reduction of P53 expression was greater in group B. Down-regulation of Bcl-2 could be enhanced when hyperthermia was combined with chemotherapy (group C) or radiation (group E), but more obvious down-regulation was found in group E as compared to group C. Hyperthermia itself could not obviously up-regulate Bax expression, and it occurred at last. Bax expression increased more by chemotherapy treatment (group B) than that by radiation (group D). By comparing to group C, the greater increase occurred in group E.
CONCLUSIONSHyperthermia enhances the effects of radiosensitivity and chemosensitivity on tumors by changing the expression of apoptosis-associated genes P53, Bcl-2 and Bax. Hyperthermia combined with chemotherapy and/or radiation has a greater effect on down-regulation of P53 and Bcl-2 expression and up-regulation of Bax expression than any single therapy.
Animals ; Apoptosis ; Cell Line, Tumor ; Cellular Apoptosis Susceptibility Protein ; metabolism ; Chemotherapy, Adjuvant ; Colonic Neoplasms ; metabolism ; therapy ; Combined Modality Therapy ; Humans ; Hyperthermia, Induced ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Transplantation ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Radiotherapy, Adjuvant ; Tumor Suppressor Protein p53 ; metabolism ; bcl-2-Associated X Protein ; metabolism
9.Distribution of hepatitis B virus genotypes in Ningxia Hui nationality and its clinical significance.
Shao-qi YANG ; Zhan-hui WANG ; Li YANG ; Jian-guo HU ; Wen-gui YANG ; Jin-lin HOU
Chinese Journal of Hepatology 2006;14(6):466-467
Adolescent
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Adult
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Child
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China
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epidemiology
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Female
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Genotype
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Hepatitis B virus
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genetics
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Hepatitis B, Chronic
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epidemiology
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virology
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Humans
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Male
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Middle Aged
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Polymorphism, Restriction Fragment Length
10.Antioxidant and antigenotoxic activity of bioactive extracts from corn tassel.
Li-chun WANG ; Ya-qin YU ; Min FANG ; Cai-gui ZHAN ; Hong-yan PAN ; Yong-ning WU ; Zhi-yong GONG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(1):131-136
This study is designed to evaluate antioxidant and antigenotoxic activities of corn tassel extracts (CTTs). The major bioactive components of CTTs include flavonoid, saponin and polysaccharide. The antioxidant properties of the three bioactive components of CTTs were investigated by Ferric Reducing Antioxidant Property (FRAP) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assays. The activities of the extracts were determined by assessing the inhibition of mutagenicity of the direct-acting mutagen fenaminosulf, sodium azide, and indirect-acting mutagen 2-aminofluorene using the Ames test (strains TA98 and TA100). The results showed that the extraction rates of flavonoid, saponin, and polysaccharide from the dried corn tassels were 1.67%, 2.41% and 4.76% respectively. DPPH and FRAP assay strongly demonstrated that CTTs had antioxidant properties. CTTs at doses of 625, 1250 and 2500 μg per plate reduced 2-aminofluorene mutagenicity by 12.52%, 28.76% and 36.49% in Salmonella typhimurium TA98 strain assay respectively and by 10.98%, 25.27% and 37.83%, at the same doses in Salmonella typhimurium TA100 assay system, respectively. 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay showed that the different concentrations of CTTs inhibited the proliferation of MGC80-3 cells in a dose-dependent manner (P<0.01). It is concluded that these integrated approaches to antioxidant and antigenotoxicity assessment may be useful to study corn tassel as a natural herbal material.
Antimutagenic Agents
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pharmacology
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Antioxidants
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pharmacology
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Biphenyl Compounds
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antagonists & inhibitors
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metabolism
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Cell Line, Tumor
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Cell Survival
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drug effects
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Flavonoids
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pharmacology
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Fluorenes
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pharmacology
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Free Radical Scavengers
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pharmacology
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Humans
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Inflorescence
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chemistry
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Mutagens
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pharmacology
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Picrates
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antagonists & inhibitors
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metabolism
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Plant Extracts
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pharmacology
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Polysaccharides
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pharmacology
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Salmonella typhimurium
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drug effects
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genetics
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Saponins
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pharmacology
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Zea mays
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chemistry