Antiviral Agents ; therapeutic use ; Guidelines as Topic ; Hepatitis B, Chronic ; drug therapy ; Humans

Hepatitis C ; etiology ; virology ; Hepatitis Viruses ; Humans ; Liver Transplantation ; Recurrence

OBJECTIVEThe postoperative presence of alpha-fetoprotien (AFP) mRNA in the peripheral blood of patient with hepatic cellular carcinoma (HCC) may suggest that carcinomatous cells still exist in the peripheral blood which may someday develop into metastasis. Therefore, the expression of AFP mRNA in postoperative peripheral blood is detected and its correlation with recurrence of HCC is investingated.

METHODSAFP mRNA in peripheral blood of HCC patients and non-hepatic cellular carcinoma patients treated by partial hepatectomy on the first, 7th, 14th postoperative day was examined by means of RT-PCR/ Southern blot. All HCC patients were followed up for 36 months.

RESULTSOf the 29 HCC patients, 18 (62.1%) had recurrence, and 17 (58.6%) was found to have AFP mRNA expression in peripheral blood during postoperative period. Fourteen of these 17 patients (82.4%) with positive AFP mRNA expression developed recurrence, but only 4 patients (33.3%) with negative AFP mRNA expression had recurrence. The difference between two groups was statistically significant (P < 0.05).

CONCLUSIONOur preliminary results suggest that detection of AFP mRNA during postoperative period may be helpful in predicting recurrence for hepatic cellular carcinoma.

Carcinoma, Hepatocellular ; genetics ; pathology ; surgery ; Female ; Follow-Up Studies ; Hepatectomy ; Humans ; Liver Neoplasms ; genetics ; pathology ; surgery ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; Postoperative Period ; Prognosis ; RNA, Messenger ; blood ; genetics ; Reverse Transcriptase Polymerase Chain Reaction ; alpha-Fetoproteins ; genetics

Adult ; Aged ; Cataract Extraction ; methods ; Endothelial Cells ; pathology ; Female ; Fuchs' Endothelial Dystrophy ; pathology ; Humans ; Male ; Middle Aged ; Phacoemulsification

In order to investigate the relationship between gut microbiota and type 2 diabetic erectile dysfunction (T2DED),we analyzed the characteristics of gut microbiota in the Sprague-Dawley (SD) rats with T2DED.Thirty-five SD rats were randomly divided into two groups:control group (n=15)with normal diet,and experimental group (n=20) with construction of T2D model.Faecal and serum samples were collected at 2nd and 8th week after establishment of T2D model,respectively.Faecal samples were used for analysis of gut microbiota,and serum samples for detection of trimethylamine N-oxide (TMAO),lipopolysaccharide (LPS),and inflammatory factors like interleukin-1 (IL-1),IL-2,IL-10,and monocyte chemoattractantprotein-1 (MCP-1).The main compositions of gut microbiota were Bacteroidetes,Proteobacteria and Firmicutes at the phylum level,and Oscillospira,Allobaculum,Bacteroides,Ruminococcus,SMB53,Prevotella,Coprococcus,Sutterella and Blautia at the genus level with relatively higher abundance in all SD rats.The relative abundance of Enterococcus,Corynebacterium,Aerococcus,Facklamia (opportunistic pathogens in most case) increased,and that ofAllobaculum,Bifidobacterium,Eubacterium,Anaerotruncus (beneficial bacteria) decreased in T2DED group as compared with that at 2nd week after establishment of T2D model (T2D2 group).The serum contents of TMAO,LPS,IL-1,IL-2,IL-10 and MCP-1 in T2DED group were significantly higher than those in control group.The gut microbiota of T2DED rats was inhibited.The gut microbiota of T2DED rats had changed,as the relative abundance of beneficial bacterium was decreased while that of opportunistic pathogens was increased.The variations of gut microbiota might lead to inflammation and prompt the emergence of erectile dysfunction in the rats with T2D.TMAO might play an important role in the formation of T2DED.

Oxidative stress is implicated in male infertility and significantly higher reactive oxygen species are detected in 25% of infertile males. Although different agents of various alternative medicines, including traditional Chinese medicine, have been tried with varying success, evidence remains limited on whether and how much herbs or supplements might help increase the anti-oxidant ability of the sperm. This study examined the anti-oxidative effects of icariin, a flavonoid isolated from Herba Epimedii, on the human sperm. We prepared the FeSO4/H2O2-damaged human sperms, which were co-cultured with icariin in vitro, and then observed the changes of the sperm by employing Raman micro-spectroscopy. The results showed that Raman mapping with a 514 nm excitation laser allowed clear differentiation of the nucleus, neck, and, in particular, the mitochondria-rich middle piece of a human sperm cell. The effect of icariin on different organelles of the sperm was quantified by localized spectral Raman signatures obtained within milli-seconds, and icariin could keep the "Raman fingerprint" of the human sperm the same as the control groups, suggesting that icariin could protect the human sperm from being damaged by FeSO4/H2O2. Icariin may serve as a tonifying and replenishing agent of herbal origin for enhancing reproductive functions.

Coinfection/drug therapy* ; HIV ; HIV Infections/drug therapy* ; Hepacivirus ; Hepatitis B/drug therapy* ; Hepatitis C/drug therapy* ; Humans ; Prevalence

Objective To investigate the implementation effect of standardized training of residents in obstetrics and gynecology.Methods A total of 40 students who participated in the standardized residency training of obstetrics and gynecology in our hospital from 2016 to 2018 were selected as respondents.The scores of professional dedication,sense of responsibility,communication skills,document writing,medical history collection,physical examination,diagnostic and differential abilities,drug use,clinical skills,and computer use were evaluated before and after training.The scores of theoretical and skill exams before and after training were collected and compared to evaluate the implementation effect of standardized training of residents in obstetrics and gynecology.Results After training,there were significant changes in the scores of professional dedication [(4.775 ± 0.366) vs.(7.475 ± 0.362),P＜0.05)],sense of responsibility [(4.800 ±0.333) vs.(7.850 ± 0.366),P＜0.05],communication skills [(4.575 ± 0.382) vs.(7.550 ± 0.339),P＜0.05],document writing [(4.833 ± 0.376) vs.(7.762 ± 0.343),P＜0.05],medical history collection [(4.762 ± 0.314)vs.(7.738 ± 0.316),P＜0.05],physical examination [(4.434 ± 0.347) vs.(7.565 ± 0.317),P＜0.05],diagnostic and differential abilities [(4.239 ± 0.274) vs.(7.348 ± 0.314),P＜0.05],drug use [(4.370 ± 0.261) vs.(8.022 ±0.246),P＜0.05],clinical skills [(4.370 ±0.330) vs.(8.217 ±0.265),P＜0.05],and computer use [(4.652 ± 0.260) vs.(8.630 ± 0.289),P＜0.05].There were also significant changes after training in the scores of theoretical exam [(70.950 ± 1.165) vs.(89.675 ± 1.164),P＜0.05] and skill exam [(67.575 ± 0.947) vs.(88.150 ± 0.877),P＜0.05].Conclusion Standardized training of residents in obstetrics and gynecology can enhance their clinical skills and improve their clinical thinking ability.

Objective:To explore the acupoint selection law in acupuncture and moxibustion in treating postpartum incontinence by using data mining technology.Methods:The clinical research literature on acupuncture and moxibustion treatment for postpartum incontinence was retrieved from CNKI, Wanfang Data, VIP, CBM, China Medical Journal Full-text Database, PubMed, Embase, Web of Science from the establishment of the databases to February 1, 2023. The acupoint prescription database was established using Excel 2016 and conducting frequency statistics of acupoints, meridians and specific acupoints. Clustering analysis was performed using SPSS Statistics 25.0, and association rule analysis was performed using SPSS Modeler 18.0.Results:A total of 70 articles were included, involving 70 acupuncture prescriptions and including 31 acupoints with a total frequency of 384 times. The top five acupoints were Sanyinjiao (SP6, 46 times), Guanyuan (CV4, 45 times), Zusanli (ST36, 43 times), Zhongji (RN3, 41 times), and Qihai (CV6, 38 times). The commonly used meridians were Conception Vessel, bladder meridian and spleen meridian. The involved acupoints were mostly located in the lower limbs and belly, and most of them were Jiaohui, Mu and Wushu acupoints. Five effective clustering groups were obtained by clustering analysis of high frequency acupoints. The core acupoint combination of association rules was Sanyinjiao (SP6)-Zusanli (ST36)-Guanyuan (CV4)-Qihai (CV6)-Zhongji (RN3).Conclusions:The pathogenesis of postpartum urinary incontinence is mainly characterized by qi deficiency. Acupuncture and moxibustion treatment of postpartum urinary incontinence follows the principles of invigorating qi and tonifying spleen, nourishing lung and tonifying kidney, paying attention to the use of acupoints in abdomen and lower limbs, and using the methods of upper and lower acupoints, anterior and posterior acupoints.

Oxidative stress is implicated in male infertility and significantly higher reactive oxygen species are detected in 25% of infertile males. Although different agents of various alternative medicines, including traditional Chinese medicine, have been tried with varying success, evidence remains limited on whether and how much herbs or supplements might help increase the anti-oxidant ability of the sperm. This study examined the anti-oxidative effects of icariin, a flavonoid isolated from Herba Epimedii, on the human sperm. We prepared the FeSO4/H2O2-damaged human sperms, which were co-cultured with icariin in vitro, and then observed the changes of the sperm by employing Raman micro-spectroscopy. The results showed that Raman mapping with a 514 nm excitation laser allowed clear differentiation of the nucleus, neck, and, in particular, the mitochondria-rich middle piece of a human sperm cell. The effect of icariin on different organelles of the sperm was quantified by localized spectral Raman signatures obtained within milli-seconds, and icariin could keep the "Raman fingerprint" of the human sperm the same as the control groups, suggesting that icariin could protect the human sperm from being damaged by FeSO4/H2O2. Icariin may serve as a tonifying and replenishing agent of herbal origin for enhancing reproductive functions.
Cells, Cultured ; Drugs, Chinese Herbal ; pharmacology ; Ferrous Compounds ; pharmacology ; Flavonoids ; pharmacology ; Flow Cytometry ; Humans ; Hydrogen Peroxide ; pharmacology ; L-Lactate Dehydrogenase ; metabolism ; Male ; Malondialdehyde ; metabolism ; Oxidants ; pharmacology ; Oxidative Stress ; drug effects ; Protective Agents ; pharmacology ; Spectrum Analysis, Raman ; Spermatozoa ; cytology ; drug effects ; metabolism ; Superoxide Dismutase ; metabolism ; Time Factors