2.Alendronate improves bone matrix structure of ovariectomized rats
Xiang WANG ; Hailing GUO ; Yongfang ZHAO ; Hongsheng ZHAN ; Yinyu SHI
Chinese Journal of Tissue Engineering Research 2013;(50):8621-8628
BACKGROUND:Bisphosphonates that can increase bone density and inhibit bone resorption have been clinical y confirmed, but the structure of the bone matrix has been less studied.
OBJECTIVE:To observe the effects of alendronate on bone structure and bone matrix metabolism, and then to investigate the control ing mechanism by which alendronate improves bone mass and increase bone intensity.
METHODS:An ovariectomized rat model was prepared and intervened with alendronate as treatment group. Model and sham-surgery groups were set as controls. Alendronate effects on bone mineral density, bone metabolism, bone biomechanics, and bone structure were observed in bone loss rats using bone imaging, bone tissue pathology and biomechanical test and enzyme-linked immunosorbent assay.
RESULTS AND CONCLUSION:Alendronate intervention could fight against bone loss as compared with model group at weeks 4, 8, and 12 after treatment (P<0.05). Compared with the model group, the expression of urinary deoxypyridinoline and serum carboxyterminal propeptide of type Ⅰ procol agen was decreased significantly after alendronate intervention (P<0.05);the maximal load, maximal pressure and modelus on the lumbar vertebrae and femur were increased as wel as ratio of urinary pyridinoline/deoxypyridinoline of type Ⅰ procol agen (P<0.05). These findings suggest that alendronate intervention can inhibit bone loss in rats induced by estrogen deficiency, increase biomechanical properties, improve bone matrix structure, and meanwhile, recover the Ⅰ col agen crosslinking component due to ovariectomy.
3.The study on 235delC mutation of GJB2 gene in patients with idiopathic sudden hearing loss.
Yue ZHAN ; Yujuan HU ; Xiang HUANG ; Huamao CHEN ; Changkai GUO ; Hongjun XIAO ; Hong SHI ; Weijia KONG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(9):621-634
OBJECTIVE:
To analyze the rate of 235delC mutation in GJB2 gene in patients with idiopathic sudden hearing loss, and to explore its possible correlation with pathogenesis of idiopathic sudden hearing loss.
METHOD:
Two hundred and thirty-four patients with diagnosis of idiopathic sudden hearing loss in otolaryngology department were recruited as experimental group. Eighty people with normal hearing level were enrolled as control group. Their peripheral blood samples were obtained and genomic DNA was extracted. Using polymerase chain reaction, the coding region of GJB2 gene was amplified, and 235delC mutation is screened for in GJB2 gene by restriction endonuclease. At same time the clinical data of 234 patients was collected to analyze.
RESULT:
In 234 cases of idiopathic sudden hearing loss, 5 cases were found to have heterozygous 235delC mutation, none of them harbored homozygous 235delC mutation, the 235delC mutation rate was 2.1% (5/234). No 235delC mutation was found in control group. The rate of 235delC mutation in two group showed no statistically significant difference (P > 0.05).
CONCLUSION
This research shows that the rate of 235delC mutation in GJB2 is low in patients with idiopathic sudden hearing loss, and suggest that 235delC mutation possible has no correlation with idiopathic sudden hearing loss.
Adolescent
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Adult
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Aged
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Child
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Connexin 26
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Connexins
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genetics
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DNA Mutational Analysis
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Female
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Hearing Loss, Sudden
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genetics
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Humans
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Male
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Middle Aged
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Mutation
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Young Adult
4.Calbindin-28 in rats of Parkinson's disease after deep brain stimulation to subthaiamus nucleus
Chun-Yan CAO ; Zhen-Guo LIU ; Sheng-Di CHEN ; Bo-Min SUN ; Shi-Kun ZHAN ; Guo-Qiang LU ;
Chinese Journal of Neurology 2005;0(10):-
Objective To explore the mechanism of deep brain stimulation(DBS)therapy to Parkinson's disease(PD).Methods We produced hemi-parkinsonian rat model with stereotaxically injecting 6-OHDA to right medial forebrain bundle(MFB)and stimulated ipsilateral subthalamu nucleus (STN)with platinum electrodes chronically to investigate the influence of DBS to the expression of Calbindin-28,synaptophysin and tyrosine dioxydase(TH)in Striatum by Western blot.In addition,slices of bilateral PD rats after DBS were stained to observe the expression of Calbindin-28 and synaptophysin in substantia nigra by Immunohistochemistry.Results High frequency stimulation impaired the rotational frequency 31% of unilateral PD rats triggered by apomophine;Long-term DBS increased the expression of TH in innocent striatum of unilateral PD rats 78.6%?9.5%,since the ipsilateral striatum(lesion side) was TH depleted by 6-OHDA insults;Calbindin-28 expression in ipsilateral striatum of hemi-PD rats raised up 75.4%?15.0% and long-term DBS reduced the effect by 43.0%?7.1%,meanwhile Calbindin-28 positive neurons in substantia nigra compacta in sham,PD and DBS rats were 74.5?10.2,75.7?15.6, 33.1?7.8.However,Synaptophysin expression in substantia nigra and striatum kept stable even after long- term DBS.Conclusions Consistent to the treatment to PD patients,DBS to STN alleviated the motor disorder of PD rats,the treatment might be based on regulating the expression of Calbindin-28 and TH.
6.Clinical experience of 1064 cases of severe acute pancreatitis: medical treatment predominant therapy
Yiqi DU ; Xianbao ZHAN ; Pei XIE ; Yuanhang DONG ; Yan CHEN ; Qihong YU ; Xiaorong GUO ; Jiefang GUO ; Wenjun ZHANG ; Xingang SHI ; Jianping LI ; Ye CAI ; Shengdao ZHANG ; Zhaoshen LI
Chinese Journal of Pancreatology 2012;(6):366-370
Objective To evaluate the value of medical treatment in the management of SAP.Methods From January 2000 to December 2011,a total of 1064 cases out of 931 SAP patients were admitted and retrospectively analyzed.The etiologies,severity score,complication rates,therapies,effectiveness and costs of those SAP cases were summarized.Results There were 559 males and 372 females with a mean age of (51 ± 15)years old.The main cause was biliary tract disease (58.3%),followed by fat-rich diet (31.2%),hyperlipidemia (13.6%) and alcohol (7.1%).At the time of admission,95.5% of SAP patients presented with level D disease according to Balthazar CT severity index,26.0% had a Ranson score ≥3 and 30.1% had an APACHE Ⅱ score ≥ 8.There were 42.7% cases complicated with systemic inflammatory response syndrome (SIRS).Acute lung injury and acute respiratory distress syndrome (ARDS),acute kidney injury,shock or heart failure,acute liver dysfunction,and diffuse intravascular clotting (DIC)occurred in 24.0%,8.1%,5.4%,3.2%,and 1% of all patients,respectively.Other complications of SAP included abdominal cavity bleeding (n =17),pseudocyst bleeding (n =9),pancreatic abscess (n =78) and gastrointestinal fistula (n =33).Totally 25 (2.3%) patients died in hospital and 36 (3.4%) patients were discharged against advice,with an overall treatment success rate of 94.3%.The mean hospital stay was (23.7 ± 19.2) d,and the average cost was 52.3 thousands of RMB.Conclusions A comprehensive treatment pathway relying on medical treatment,focusing on organ function support and assisted by miniinvasive intervention may improve the treatment success rate of SAP,which is worth of further application.
7.Related factors of vision recovery after complex traumatic and non - traumatic retinal detachment
Jiang-Bo ZHAN ; Shi-Ming CHENG ; Shu-Yun GUO ; Yun-Fang ZHANG ; Rui GUO
International Eye Science 2018;18(7):1271-1274
·AIM: To investigate the visual recovery factors in patients with complex traumatic and non-traumatic retinal detachment. ·METHODS: According to the history of ocular trauma before admission, 135 patients with complex retinal detachment were divided into traumatic group ( n=66, 66 eyes) and non-traumatic group (n=69, 69 eyes). The results of visual recovery and complications were compared between the two groups. Logistic regression was used to analyze the related factors of visual recovery in the two groups. ·RESULTS: There was no significant difference in the recovery rate between the two groups within 24h after surgery (77. 3% vs 78. 3%, P>0. 05), and there was no significant difference in the total incidence of complications at 3mo after surgery(18. 2% vs 17. 4%, P>0. 05). Multifactorial Logistic regression analysis showed significant correlation between age, injury type, time since retinal detachment, rage of retinal detachment, preoperative vitreous blood,proliferative vitreoretinopathy ( PVR ) and postoperative visual recovery in patients with complex traumatic retinal detachment ( P < 0. 05 ); age, time since retinal detachment, rage of retinal detachment and macular status were significantly associated with visual recovery in patients with complex non-traumatic retinal detachment (P<0. 05). ·CONCLUSION: Age, time since retinal detachment and rage of retinal detachment were significantly associated with traumatic and non-traumatic retinal detachment. The injury type, preoperative vitreous hemorrhage, PVR were significantly correlated with the visual recovery of traumatic retinal detachment patients. The condition of macular was significantly associated with the visual recovery of non-traumatic retinal detachment patients.
8.Predictive efficacy of magnetic resonance spectroscopy in patients with glioblastoma multiforme during and after postoperative radiotherapy with concomitant/adjuvant temozolomide
Chaofeng LIANG ; Haoqiang ZHAN ; Zhongxing LUO ; Ning GUO ; Jianqiang SHI ; Bocheng WANG ; Ying GUO
Chinese Journal of Neuromedicine 2017;16(11):1147-1152
Objective To explore the predictive ability of magnetic resonance spectroscopy (MRS) in overall survival (OS) and progression-free survival (PFS) of patients with glioblastoma multiforme (GBM) before,during,and 2 months after radiotherapy with concomitant/adjuvant temozolomide (TMZ).Methods GBM patients,admitted to our hospital from January 2011 to January 2016 and confirmed by pathology,were chosen in our study;all patients underwent postoperative three-dimensional conformal radiotherapy with concomitant/adjuvant TMZ.And 3D-MRS was performed before,during,and 2 months after radiotherapy,the levels of N-acetyl-aspartic acid (NAA),choline (Cho) and creatine (Cr),and ratios of Cho/NAA,Cho/Cr and NAA/Cr in the GBM/edge of surgery side and the normal brain tissues were observed.The survival curve,median overall survival (mOS) and median progression free survival (mPFS) of patients with standardized Cho decreased<30% and patients with standardized Cho decreased>30% 2 months after radiotherapy were compared.Results Twenty-one patients finished the scheduled MRS for 3 times.Until the end of our study,16 patients died and 5 survived.Standardized Cho gradually decreased before,during,and 2 months after radiotherapy (2.08±0.22,1.45 ±0.21 and 1.16±0.18),with significant differences (P<0.05).Standardized Cho after radiotherapy was significantly decreased as compared with that before radiotherapy (P<0.05).Ratios of Cho/NAA and Cho/Cr in the GBM/edge of surgery side were significantly higher than those in the normal brain tissues (P<0.05),and ratio of NAA/Cr in the GBM/edge of surgery side was significantly lower than that in the normal brain tissues (P<0.05).Ratio of Cho/NAA gradually decreased before,during,and 2 months after radiotherapy,with significant differences (P<0.05).As compared with patients with standardized Cho decreased<30% 2 months after radiotherapy,patients with standardized Cho decreased>30% 2 months after radiotherapy had significantly decreased rates of OS and PFS,and statistically shorter mPFS and mOS (4.5 vs.13.5,10.9 vs.25.3,P<0.05).Conclusion The changes of standardized Cho 2 months after radiotherapy have high prognostic significance for PFS and OS.
9.Systematic review of clinical randomized controlled trials on manipulation treatment for vertebral artery type of cervical spondylosis.
Kai GUO ; Lin LI ; Hong-Sheng ZHAN ; Hui-Hao WANG ; Yin-Yu SHI
China Journal of Orthopaedics and Traumatology 2012;25(1):9-13
OBJECTIVETo evaluate the safety and efficacy of the manipulation treatment for vertebral artery type of cervical spondylosis and analyze the current status of clinical study.
METHODSTo retrievie the PubMed, OVID, Cochrane library, CBM-disc database, CNKI database, VIP database, 265 literatures of the manipulation treatment of vertebral artery type of cervical spondylosis were collected, including 251 literatures Chinese and 14 literatures English. Among the literatures, 5 studies of Chinese literatures accorded with inclusion criteria. Cochrane systematic review was used to quality evaluation; and 5 studies were used RevMan 4.2 for Meta analysis of literature.
RESULTSFive documents included a total of 736 patients. Summary of the literature combined OR for the cure rate was 2.93, 95% CI was [2.10,4.08]. Summary OR for the combined efficiency was 3.99, 95% CI was [2.47,6.44]. Manipulation group's cure rate of vertebral artery type of cervical spondylosis was efficient and clearly superior to the other therapies. Expect of the manipulation, the traction and the drugs, hereinafter referred to as "other therapies" (P < 0.01).
CONCLUSIONAt present, documents included in this study have shown that manipulation treatment on vertebral artery type of cervical spondylosis is safe, both cure rate and the effective rate are much better than other therapies; but the incorporation of a limited number of documents and the quality is not very high, the conclusion is still uncertain, high-quality evidence should be needed to be further validated.
Humans ; Manipulation, Spinal ; methods ; Randomized Controlled Trials as Topic ; Spondylosis ; therapy ; Vertebral Artery
10.The results of cementless Zweymüller hip system: 5 to 11 years follow-up study.
Yu-li GUO ; Zhan-jun SHI ; Da-di JIN ; Zong-sen JING ; Jian WANG ; Zhi-gang ZHU
Chinese Journal of Surgery 2009;47(13):1020-1023
OBJECTIVETo analyze the intermediate-term results associated with the use of Zweymtiller hip system.
METHODSReview the 116 cases (142 hips) who were treated with total hip replacement from 1996 to 2002 by a single surgeon using cementless Zweymüller hip systems.
RESULTSSixty-one cases (77 hips) were followed up, 50 cases (64 hips) were evaluated both clinically and radiographically while 5 cases (6 hips) and 6 cases (7 hips) were only evaluated clinically and radiographically respectively. The average follow-up period was 7.3 years (range 5 to 11 years). The mean preoperative Harris Hip Score was 46 while the mean postoperative Harris Hip Score was 93. Distal cortical hypertrophy and medullary sclerosis were observed in 30 hips (42.3%) and 33 hips (46.5%) respectively. Heterotopic ossification arose in 45 hips (63.4%). Radiolucent lines occurred in 27 stems (38.0%) but in no cups. Radiolucent lines were mostly observed in Gruen zones 1. Osteolysis occurred in 7 cups (9.9%) and 18 stems (25.4%). Osteolysis was mostly observed in Delee Zone 3 and Gruen zone 7. In the distal Gruen zones 3, 4 and 5, no radiolucent line or osteolysis was observed. No hips had been revised, 3 cups needed revision surgery because of aseptic loosening.
CONCLUSIONThe 5-11 years follow-up results are satisfactory, but osteolysis is common.
Adolescent ; Adult ; Aged ; Arthroplasty, Replacement, Hip ; Female ; Follow-Up Studies ; Hip Prosthesis ; Humans ; Male ; Middle Aged ; Treatment Outcome ; Young Adult