OBJECTIVE:To establish a method for the content determination of naproxen in naproxen-?-cyclodextrin inclusion complex.METHODS:The drug concentration in naproxen-?-cyclodextrin inclusion complex was detected at the wavelength of330nm by using UV spectrophotometry.RESULTS:The linear range of naproxen was2.0～80.0mg/L(r=0.9995,n=5),the average recovery was99.22%(RSD=1.03%).CONCLUSIONS:The method is simple and reliable,which can be used for the content determination of naproxen in naproxen-?-cyclodextrin inclusion complex.

Antibodies, Monoclonal ; immunology ; therapeutic use ; Antibodies, Monoclonal, Humanized ; Carcinoma, Squamous Cell ; drug therapy ; Cetuximab ; Head and Neck Neoplasms ; drug therapy ; Humans ; Receptor, Epidermal Growth Factor ; immunology

BACKGROUND:Based on variational principle and weighting technology of three-dimensional finite element method, human teeth with a complex morphology can be modeled, which helps to understand the stress distribution of dental hard tissue and prosthesis during the dynamic repair process. OBJECTIVE:To comprehensively analyze the three-dimensional finite element studies concerning biomechanics of dental prostheses, focusing on the effects of metal crown, porcelain thickness, root canal preparation and fil ing on the tooth stress. METHODS:A computer-based search of PubMed (1993-04/2012-09), China Academic Journal Network Publishing Database (2001-2008), and VIP (2001-2008) was performed by the first author to retrieve articles concerning the effects of metal crown, porcelain thickness, root canal preparation and fil ing on the tooth stress. The keywords were“porcelain-fused-to-metal, finite element method, stress analysis, root canal”in English and Chinese. Articles with repetitive contents or meta-analysis were ruled out. Then 40 articles were suitable for further analysis.RESULTS AND CONCLUSION:Finite element method has important significance to establish high-fidelity and high-accuracy models in oral medicine, thereby providing effective biomechanical information for the root canal treatment and post-treatment repair. Scholars continue to explore the stress distribution of dental prostheses during chewing. This review summarizes the stress changes of post and core crowns, supporting reference for further research. Three-dimensional finite element method can be used to build nonlinear three-dimensional finite element models with anisotropic biomechanical characteristics, and can gradual yimprove the transition from static analysis to a dynamic analysis, truly achieving accurate simulation of oral biology and dental morphology as wel as chewing function of the teeth.

This study was aimed to evaluate the effectiveness of solution form of 17% ethylenediaminetetraacetic acid (EDTA) on removing smear layer of root canals at different exposure time periods and to provide scientific basis for EDTA as a choice of root canal irrigation in clinical practice. Twenty-five single-rooted teeth were randomly divided into 5 groups: control group (group A) was given 2.5% NaOCl, and 4 experimental groups were given 2.5% NaOCl and 17% EDTA, including groups B, C, D and E with exposure time of 1, 3, 5 and 7 min, respectively. After preparation of the root canals, the teeth were split along their longitudinal axis, and the root sections were examined under scanning electron microscope for evaluation of smear layer removal and erosion on the surface of the root canal walls. The specimens in group B showed presence of smear layer on the walls of the root canal with no statistical difference from that in group A (P>0.05). In groups C and D, partial removal of smear layer was obtained, and there was no significant difference between the two groups (P>0.05), but there was significant difference in removal of smear layer between group C and group B (P<0.05). Root canal walls in group E specimens showed almost complete removal of smear layer, and the removal of smear layer was significantly different from that in group D (P<0.01). There was no significant change in the structure of the surface of root canal for each sample. It was concluded that combined irrigation with 17% EDTA and 2.5% NaOCl could remove the smear layer with no significant alteration in dentinal structure when the chelating agent was applied for 7 min. At 3 and 5 min of application, partial removal of smear layer was observed and at 1 min negligible removal of smear layer was achieved.

OBJECTIVE To explore the risk factors and protective measures on ICU attendants.METHODS According to the risk factors of ICU occupational exposure,we developed and standardized the implementation of the protective measures.RESULTS The occupational exposure protection awareness,prevention methods,and self-protection were improved.CONCLUSIONS The education about occupational of exposure can improve the management of occupational safety and ensure the protection of physical and mental health of attendants,and to prevent the occurrence of hospital infection.

Objective To investigate whether KAP-1 could induce CSC-self renewal in pancreatic cancer cell lines.Methods KAP-1 expression was examined in 14 cases of pancreatic cancer with immunohistochemistry.KAP-1 shRNA amplified by PCR was inserted into pGC-LV in vector,and then identified by restriction endonuclease digestion and nucleotide sequencing.The lentiviral vector pGC-shRNA-KAP-1 was co-transfected with pHelper 1.0 and pHelper 2.0 packaging plasmids into HEK 293T cells,and the lentivirus was collected and virus titer was measured.The expressions of KAP-1 and vimentin were detected by Western blot and RT-qPCR when human pancreatic cancer cell line Panc-1 was infected by the lentivirus.Sphere forming assay was conducted to assess the capacity of CSC or CSC-like cell self-renewal in this study.Results The KAP-1 expression level in cancerous tissues on immunohistochemistry was significantly higher than in the corresponding normal tissues (P=0.002).After infected by lentivirus,the expressions of KAP-1 and vimentin were knocked down,which could be detected by Wesren blot and RT-qPCR.Compared to Panc-1-GFP (NC) cells,the outcomes suggested that knocking down the expression of KAP-1 could decrease the formation of pancreatospheres,which further suggested the capacity of CSC-self-renewal in primary and secondary pancreatospheres of Panc-1-shRNA-KAP-1 and NC cells.Conclusions KAP-1 expression in pancreatic cancer tissues has been identified.The lentiviral vector for shRNAs targeting KAP-1 was constructed successfully.The formation of pancreatospheres decreased by knocking down the KAP 1 gene.KAP-1 is involved in the regulation of CSC phenotype.The mechanism is probably related to the upregulated expression of the EMT marker vimentin.

Medical care on the battlefield is the core and basis of echelons of care.This review summarizes the background and characteristics of medical care units on the battlefield from the birth and growth of mobile army surgical hospitals before being replaced by forward surgical teams and combat support hospitals, since the United States Armed Forces began to lead the world military revolution during and after the World WarⅡ.Quick adaptation to the combat envi-ronment and the combat modes is the main reason that medical care units on the battlefield are adjusted continuously.This paper may provide some ideas for the development of our medical care units on the battlefield in the future.

Investigation of the corporate governance at the pilot areas along with literature review of the outcomes of corporate governance reform;reasonable definition of the authority-responsibility relationship of the stakeholders and the impact on the corporate governance model;optimization of public hospital′s corporate governance to recommend an optimal design of such hospitals.Interests demands of stakeholders were considered as a key factor in the design of such governance;roles of the stakeholders in such governance were enhanced,to clarify the distribution of ownership,decision-making power and management rights,with widened supervision and narrowed management radius.

Objective To analyze the clinical characteristics and resistance of group B hemolytic streptococcus septicemia in neonates,and to provide the evidence for clinical treatment.Methods 7 neonates with group B hemolytic streptococcus septicemia hospitalized from Jan.2001 to Aug.2011 were enrolled in this study.The clinical data and laboratory results of antimicrobial sensitivity were analyzed retrospectively.Results There were 4 cases of early-onset infection and 3 cases of late-onset infection,6 cases with leukopenia,6 cases with c-reactive protein elevated,4 cases with suppurative meningitis,3 cases with septic shock and 2 cases with disseminated intravascular coagulation.All strains were sensitive to penicillin, vancomycin and linezolid.Conclusion Attention should be paid to neonatal group B hemolytic streptococcus septicemia.Penicillin and vancomycin are first choice for this type of infection.

Objective To investigate the X-ray,CT and MRI features of peripheral primitive neuroectodermal tumors (pPNETs).Methods 14 patients were pathologically diagnosed with pPNETs.The imaging of X-ray (twelve image),Computed Tomography(eleven image) and Magnetic Resonance Imaging(five image) were retrospectively analyzed.Results Of 14 patients,there were two sites of tumour:soft tissue( eight patients) and bones( six patients).For tumoura of the soft tissue region,the appearance of X-ray and CT imaging showed equal or lower density soft mass without dear boundary.In some cases,tumours invaded adjacent bones.The appearance of MRI imaging showed large heterogeneous masses inside soft tissue without clear boundary.Tumours displaced adjacent cortical bone and cavitas medullaris.All masses were heterogeneous enhancement on contrast images.For tumours of bone region,X-ray and CT scan showed large scale osteolysis osteoclasia in bone surrounding with soft tissue masses of various size.The lesions were equal or lower signal on T1 WI.All masses were intermediate or hyperintense signal with heterogeneous midrange or obvious enhancement on T2WI.Conclusion Imaging characteristics of pPNETs were described.X-ray,CT and MRI were useful in understanding the property,extent and staging of tumor and very useful in the diagnosis.