Objective To investigate the prognostic factors for patients with hepatitis B virus-related acute-on-chronic liver failure,and to provide a basis for clinical diagnosis and treatment.Methods A total of 172 patients with hepatitis B virus (HBV)-related acute-on-chronic liver failure who were admitted to The First Hospital of Jilin University from January 1,2006 to January 1,2016 and had complete medical records and follow-up data were enrolled,and a retrospective analysis was performed for their clinical data and laboratory markers to determine prognostic factors.The independent-samples t test was used for comparison of continuous data between groups,the chi-square test was used for comparison of categorical data between groups,and a multivariate logistic regression analysis was performed for the indices determined to be statistically significant by the univariate analysis to screen out independent risk factors for the prognosis of patients with HBV-related acute-on-chronic liver failure.Results The multivariate logistic regression analysis was performed for the indices determined to be statistically significant by the univariate analysis,and the results showed that the prognostic factors were total bilirubin (TBil),prothrombin time activity (PTA),Na +,total cholesterol (TC),Child-Turcotte-Pugh (CTP) score,age ≥50 years,the presence of liver cirrhosis,bilirubin-enzyme separation,and complications.The multivariate regression analysis was performed for the complications determined to affect prognosis by the univariate analysis,and the results showed that the complications as risk factors were hepatic encephalopathy,hepatorenal syndrome,and infection.Conclusion TBil,PTA,Na +,TC,CTP score,age ≥50 years,the presence of liver cirrhosis,bilirubin-enzyme separation,and complications are independent risk factors for the prognosis of patients with HBV-related acute -on-chronic liver failure.Liver failure patients with hepatic encephalopathy,hepatorenal syndrome,and infection tend to have poorer prognosis.Therefore,early judgment of the prognosis of patients with liver failure is of great importance in the prevention and treatment of related complications.

Objective To explore the role of PADI4 mRNA in the initiation and development of rheumatoid arthritis(RA)and its correlation with clinical features.Methods Fifty-three RA patients,27 OA patients and 30 healthy volunteers were included in the study.The real-time-fluorescence quantitative PCR method was used to measure the expression level of PADI4 mRNA.The level of Anti-CCP antibody and DAS 28 of the RA patients were measured at the same time.Results It was shown that the level of PADI4 mRNA from RA was significantly higher than that of the OA and control group(P

ObjectiveTo investigate whether single nucleotide polymorphism (SNP) of macrophage migration inhibitory factor (MIF) gene - 173G/C is associated with severe preeclampsia.Methods Totally 124 severe preeclampsia patients and 160 healthy pregnant women (control group) were included in our study who were recruited consecutively from Affiliated Hospital of Qiugdao University Medical College between March 2010 and March 2011.The SNP was detected through SYBR Green PCR.The levels of fasting blood glucose ( FBG),fasting insulin ( FIN),and serum total cholesterol (TC),triglyceride ( TG),high density lipoprotein (HDL) and light density lipoprotein (LDL) were determined in every participants.The homeostasis model assessment-insulin resistance (HOMA-IR) was calculated.The allele and genotype frequencies between severe preeclampsia patients and control group were compared.The FBG,FIN,body mass index (BMI),HOMA-IR,TC,TG,HDL and LDL in different genotype were compared.Results ( 1 ) The MIF - 173G/C SNP genotype frequencies of GG,CG,and CC were 62.1％ (77/124),30.6％ (38/124),7.3％ (9/124),the allelic frequencies of G and C were 77.4％ ( 192/248 ) and 22.6％ (56/248),respectively,in severe preeclampsia patients; the MIF - 173G/C SNP genotype frequencies of GG,CG,and CC were 64.4％ ( 103/160 ),30.6％ (49/160),5.0％ ( 8/160),the allelic frequencies of G and C were 79.7％ (255/320) and 20.3％ (65/320),respectively,in the control group.No significant differences were observed in the genotypes and allele distributions of MIF - 173G/C SNP between the severe preeclampsia patients and control group (all P ＞ 0.05 ).(2) The severe preeclampsia patients with CG and CC genotypes had higher BMI compared with the GG genotype [ (25 ±4) versus (22 ±4) kg/m2 ; t =3.96,P ＜ 0.05 ].( 3 ) The severe preeclampsia patients with CG and CC genotypes had higher FIN level and higher HOMA-IR compared with the GG genotype [ ( 15.7 ±2.9) versus ( 13.6 ±4.0) mmoL/L,3.3 ±0.5 versus 2.7 ± 0.6 ; t =3.17,t =5.58,all P ＜ 0.05 ].(4) There was no significant difference in FBG,TC,TG,HDL and LDL levels in severe preeclampsia patients with different genotypes (all P ＞0.05 ).Conclusions The present study suggests that the MIF - 173G/C SNP is associated with insulin resistance in severe preeclampsia patients.The CG and CC genotypes increase the degree of insulin resistance,but it is may not associate with susceptibility among severe preeclampsia patients of Han Chinese women.

Acetyl-CoA C-Acyltransferase ; deficiency ; Amino Acid Metabolism, Inborn Errors ; diagnosis ; enzymology ; metabolism ; Diagnosis, Differential ; Humans ; Infant ; Isoleucine ; metabolism ; Male

Objective To investigate the association between single nucleotide polymorphism (SNP) of macrophage migration inhibitory factor (MIF) gene-rs1007888 and the pathogenesis of gestational diabetes mellitus (GDM).Methods A total of 120 GDM pregnant women (GDM group) and 165 healthy pregnant women (control group) from Affiliated Hospital of Medical College,Qingdao University were recruited from June 2011 to July 2012.Their age,gestational week,height and weight were recorded.The levels of fasting blood glucose (FBG) and fasting insulin (FIN) were determined.Body mass index (BMI),the hemeostasis model assessment-insulin resistance (HOMA-IR) and hemeostasis model assessment-β cell function (HOMA-β) were calculated.DNA was extracted from fasting blood samples.SNP of MIFrs1007888G/A was determined by DNA sequencing.The FBG,FIN,HOMA-IR and HOMA-β were compared between GDM group and the control group.They were also compared among pregnancies withdifferent genotypes.Results (1) GDM group had higher FBG,FIN and HOMA-IR levels,but lower HOMA-β than the control group (all P ＜ 0.05).(2) MIF-rs1007888 SNP genotype frequencies of GG,GA and AA were 37.5％,45.8％ and 16.7％,and the allelic frequencies of G and A were 60.4％,39.6％ in GDM group; However,in the control group,the frequencies of GG,GA and AA were 26.1％,54.5％ and 19.4％,and the allelic frequencies of G and A were 53.3％,46.7％,respectively.The distributions of MIF genotypes in GDM patients were significantly different from the healthy subjects (P ＜ 0.05).No significant difference of MIF-rs1007888 allele distributions was observed between GDM group and the control group (P ＞0.05).(3) The FBG,FIN and HOMA-IR in pregnant women with GG genotype were statistically higher than those with GA or AA genotypes,while HOMA-β was lower in women with GG genotype (all P ＜0.05).Conclusions The SNP of MIF rs-1007888 was related to the insulin resistance and pancreatic β cell function of pregnant women.GG genotype of MIF-rsl007888 might be a genetic susceptible factor in the pathogenesis of GDM.

Objective To investigate the clinical characteristics and treatment of acute postrenal acute renal dysfunction associated with ceftriaxone.Methods Twenty-five cases of the ceftriaxione-associated acute postrenal renal insufficiency were reviewed.There were 16 males and 9 females,mean age 28years.The serum contents of BUN and Cr were ( 18.6 ± 7.0) mmol/L and (635.5 ± 248.7 ) μmol/L,respectively.All patients were divided into two groups depending on the therapy:11 patients accepted the drug therapy (alkalinization of the urine,antispasmodic,etc) and 14 patients accepted the intraureteral cannula.The clinical characteristics and the treatment effect were compared between the 2 groups.Results The patients of the intraureteral cannula group ( 1.4 ± O.7 d) went to hospital earlier than the drug therapy group (3.0 ± 1.4 d) ( P =0.045 ) after the symptom of oliguria or anuria appeared.There were no significant differences in serum creatinine,urea nitrogen,and the age between the 2 groups ( P ＞ 0.05 ).All the patients were cured after treatment.There were no significant differences in recovery time (2.9 ± 1.1 d and 3.2 ± 1.2 d,P =0.963) and hospitalization time (7.0 ±2.3 d and 5.9 +3.9 d,P =0.568) between the 2 groups.Conclusions The acute renal failure associated with ceftriaxone should have high attention.The prompt medical attention,including the intraureteral eannula and the drug therapy,can both achieve the satisfying curative effect.

BACKGROUND: Retinitis pigmentosa (RP) is a non-inflammatory, bilaterally progressive, retinal degeneration characterized by loss of photoreceptor cells via an apoptotic mechanism, and it eventually leads to blindness.Research shows that the traditional Chinese medicines of Astragalus has great prospect on blocking the progression of RP disease.OBJECTIVE: To observe the protective effect of Astragalus on N-methylN-nitrosourea (MNU)-induced retinal damage in Sprague-Dawley (SD) rats and provide the optimal treatment for RP in humans.DESIGN: Randomized controlled experiment.SETTING: School of Pharmaceutical Sciences, Xinxiang Medical College.MATERIALS :The experiment was completed in Pharmacological Laboratory of Zhongshan Ophthalmic Centre, Sun Yat-sen University between March to December 2004. Totally 114 female SD rats were purchased from the Animal Center of Zhongshan Medical College, Sun Yat-sen University.MNU was purchased from Sigma Company of America. Astragalus injection was purchased from Chengdu Diao Jiuhong Pharmaceutical Factory (Batch No. Z99060535, 2 mL/ampoule, main ingredient: Astragalus).METHODS: Among 114rats, 30 were for morphometric analysis of retinal layers, 30 were for detection of apoptosis and 54 were for detection of NF-κB p65 activity. All of them were randomly divided into different groups and each group had 6 rats. Astragalus at doses of 2.5, 5 and 10 g/kg were injected intraperitoneally into 47-day rats once a day. Meanwhile, a single intraperitoneal injection of 60 mg/kg MNU was given to 50-day rats in model and Astragalus groups. At different intervals after MNU treatment,the animals were sacrificed. Retinal damage was evaluated based on retinal thickness, the apoptotic index of the photoreceptor cells was detected by TUNEL labeling and the DNA-binding activity of NF-κB p65 was analyzed according to transcription factor assay kit.MAIN OUTCOME MEASURES: Comparison of retinal thickness, apoptotic index and the activity of nuclear NF-κB p65.RESULTS: Totally 114 rats entered the result analysis. Pretreatment with Astragalus could dose-dependently suppress MNU-induced photoreceptor cell loss and decreased the apoptotic index. Astragalus at dose of 10 g/kg also time-dependently up-regulated the activity of nuclear NF-κB p65.However, protective effect of Astragalus on MNU-induced central retinal damage was not found.CONCLUSION: Astragalus partially protects against MNU-induced retinal damage by up-modulating the activity of nuclear NF-κB p65 to inhibit apoptosis of photoreceptor cells in a dose-dependent manner.

Objective To construct extravillous trophoblast(EVCT) tissue microarray and detect the expression of phosphorylated signal transducer and activator of transcription 3 (pStat3) in EVCT and to explore the role of Stat3 signal transduction pathway in the pathogenesis of preeclampsia.Methods Placentas of 80 pregnant women with preeclampsia and 58 normal pregnant women hospitalized in the Third Affiliated Hospital of Zhengzhou University from December 12,2007 to December 31,2010 were recruited for constructing EVCT tissue microarray.Vimentin,cytokeratin and human leukocyte antigen-G were used to verify EVCT tissue microarray immunohistochemically.The difference of pStat3 expression was detected between preeclampsia patients and normal pregnant women by immunohistochemical staining.Rank sum test,Kruskai-Wallis H test,t-test and Chisquare test were used for statistical analysis.Results Placental tissues from 57 preeclampsia patients (109 tissue cores) and 31 normal pregnant women (65 tissue cores) were suitable for constructing EVCT tissue microarray.The target tissue was positive for both cytokeratin and human leukocyte antigen-G staining and negative for vimentin,which was in accordance with the characters of EVCT tissue.Totally 86.4％(76/88) samples retained the target EVCT tissues,which meant EVCT tissue microarray was constructed successfully.The expression of pStat3 was significantly decreased in EVCT of preeclampsia patients (51.1％,24/47),the early onset (50.0％,19/38) and severe preeclampsia patients(52.3％,23/44) as compared to normal pregnant women (72.4％,21/29) (U=492.00,473.00 and 401.00,P＜0.05 respectively).Conclusions EVCT tissue microarray has been successfully constructed,and could be used to detect pStat3 expression.pStat3 signal transduction pathway may be involved in the development of preeclampsia.

Objective To investigate the role of nurses in civil cardiac death organ donation work.Methods Cooperating,propagating and promoting in civil cardiac death organ donation; building a bridge between the families of organ donation,donor coordinators,physicians and hospital ethics committees; participating in organ donation medical ethics assessment work; cooperating with the doctors do intend to maintain organ donation,access and preservation; protecting donors remains.Results During the time of July 2011 to November 2013,we successfully completed 52 cases of civil cardiac death organ donation cooperating with doctors,and got 41 liver,which entered the national organ allocation system for distribution.Conclusions The nurses will play a more and more important role in work of civil cardiac death organ donation cooperating with doctors.

Objective To explore influencing factors for complete resection and operation time of endoscopic submucosal dissection( ESD) for colorectal tumors. Methods This retrospective study included 95 consecutive colorectal tumors in 88 patients whose pathological diagnosis was adenoma and carcinoma, treated with ESD at the Department of Endoscopy of the First Hospital of Jilin University from January 2013 to December 2014. Multiple logistic regression analysis was conducted on the factors related to complete resection and operation time. Results Average tumor size was 28. 7±14. 1 mm(range,8?80 mm), and the average procedure time was 80. 72±63. 90 min. The rate of complete resection was 92. 6%(88/95),and the rate of incomplete resection was 7. 4%(7/95). Multivariate logistic regression analysis revealed that fibrosis (P=0. 012,OR=52. 473, 95%CI:2. 571?1140. 438) contributed to incomplete resection. Fibrosis ( P=0. 001, OR=0. 045, 95%CI:0. 007?0. 289) ,tumor size ( P=0. 035,OR=0. 170, 95%CI:0. 033?0. 884) ,granular?type laterally spreading tumor ( P=0. 013, OR=34. 432, 95%CI:2. 138?554. 476 ) , non?granular?type laterally spreading tumor(P=0. 044,OR=31. 715, 95%CI:1. 093?919. 904) were independent factors for extending operation time of colorectal ESD. Conclusion The severer fibrosis can induce higher rate of incomplete resection. The more severe fibrosis is, the larger tumor size is, and the longer operation time is.