Objective: To determine phellodendrine in Cortex Phellodendri by HPLC. Methods : HPLC condition consists of C 18 column (Phenomenex, 150mm, 5?), actonitrile:250mL of 0.05mol?L -1 phosphoric acid +0.4mL of diethylamine (9∶91, v/v) as mobile phase, detection wavelength at 284nm. Results : The averagy recovery of phellofendrine was 101.43% ( RSD =1.56%, n =5) and the linear range of phellodrine was 0.4368～2.1840?g??L -1 , r =0.9998. Conclusion : The method is simple, accurate and reproducible, and can be used for the determination of phellodendrine in Cordex Phellodendri.

Objective To improve the detail of the surgery for cicatricial ectropion,and the the effect was investigated. Methods Based on 46 patients withⅡ,Ⅲdegree cicatricial ectropion admitted to our department,choose the junction of normal skin and scar tissue as the incision line according to the degree of ectropion and local situation,and to reserve and recover the orbicularis oculi muscle as possible. The released areas exceeded separately the external canthus and reached the level of eye fissure,take advantage of full-thickness skin graft to correct the parallel lines and make fixation to the eyelid ectropion fusion skin graft on external canthal ligament. Results After followed up for 6 months to 2 years,46 cases of eyelid skin grafts survived completely and eyelids were in good reduction,with none case of incomplete eyelid closure. Conclusion Choose the surgical incision and the released areas in a proper way to reserve the structural integrity of orbicu-laris muscle and make skin graft fixation to inner and external canthal skin can prevent effectively the recurrence of ectropion and improve surgical progress.

Objective: The effects of purple-fleshed sweet potato anthocyanin (PSA) on anti-oxidation and anti-aging were investigated. Methods: The effects of different doses of PSA(100、 500、1000 mg/kg bw)on the serum T-AOC were investigated in aged mice after administration for 3 d, 10 d and 18 d. The content of MDA, the activity of serum SOD and blood GSH-Px were measured, and compared with those in Vit E and control group after 30 d. Result: PSA significantly increased the serum T-AOC in aged mice, and this increment was higher when given longer. PSA significantly decreased MDA and enhanced SOD and GSH-Px activities. The efficiency on antioxidative capacity of aged mice at PSA 100 mg/kg bw was equivalent to that of vitamin E at the same amount (100 mg/kg bw). The antioxidation state of 13 mo mice given PSA at 1000 mg/kg bw was near to that of 4 mo mice. Conclusion: PSA has marked effect of antioxidation and can delay aging.

Objective To observe the alteration of capillary density and apelin/APJ expression in soleus muscles of high-fat diet rats.Methods Male 5-week-old Sprague-Dawley rats were randomly divided into a control group and a high-fat diet group.After 12 weeks of high-fat diet,16 rats were se lected and randomly divided into a sedentary group and a treadmill running group.The exercise rats underwent 60-minute treadmill running at 26 m/min 5 days a week for 10 weeks.The body weight,body fat and blood lipid level were measured for all rats.The protein expression of Soleus CD31 and apelin was determined using immunohistochemical staining,soleus apelin content was determined using the radioimmunoassay,and the mRNA expression of apelin/APJ was detected using real-time PCR.Results Compared with the control group,significant increase was observed in the body weight,body fat and the level of total triglyceride,total cholesterol and low density lipoprotein cholesterin,but significant decrease was found in the high density lipoprotein cholesterin in the high-fat diet group.There were no significant differences in the capillary density and mRNA levels of apelin/APJ between the two groups.Compared with the sedentary high-fat diet group,significant improvement was observed in the body weight and blood lipid level of the treadmill running group.Moreover,significant increase was also observed in the capillary density,the expression of apelin/APJ mRNA,as well as that of apelin protein in the treadmill running group (P＜0.05).Conclusion The treadmill running can significantly increase capillary density of obese rats,as it may activate the expression of Apelin/APJ.

Objective:To detect immunological effects of procyanidin ,gastrodin,baicalein and apigenin on antigen presenting cells of RAW264.7 and DC2.4 and search for new tumor vaccine adjuvant.Methods: After different concentrations of four kinds of Chinese herbal monomer and LPS were used to stimulate RAW 264.7 or DC2.4 cells for 48 hours,cells were obtained and stained with CD80,CD86,MHCⅠand MHCⅡ antibody.Then protein expression levels of CD80,CD86,MHCⅠ and MHCⅡ on RAW264.7 and DC2.4 cells were analyzed by flow cytometry.Results: Results showed that after 48 hours stimulated with different concentrations of four kinds of Chinese herbal monomer on RAW 264.7 or DC2.4 cells,the expression levels of CD80,CD86,MHCⅠ,MHCⅡ were all up-regulated in comparison with control , procyanidin and baicalein exhibited stronger immunological stimulating activity on a cellular level.Conclusion:Procyanidin and baicalein were found having potential application value in clinical treatment as tumor vaccine adju -vants.

BACKGROUND: It remains unclear whether ectomesenchymal cells also derived from neural crest stem cell in mammals.OBJECTIVE: To understand the specific markers and differentiation directions of maxillofacial and mandibular processes progenitor cells,and to explore the source and differentiation phenotype of ectomesenchymal stem cells.METHODS: The expression and changes of expression profiles of rat ectomesenchymal cells at E9.5,E10.5,E11.5,and E12.5days were observed by immunohistochemistry and flow cytometry.RESULTS AND CONCLUSION: The progenitors expressed multi-lineage markers,including neural system and several rnesenchymal tissue types,importantly the facts that molecule profiles were changed with time prolonged,suggesting these progenitors were in active differentiating stage,so they were stem like cells or contain stem like cells.Moreover,small populations(2%-3%)of CD57 and P75 phenotypes were detected by flow cytornetry,suggesting that ectomesenchymal stem cells were derived from neural crest,which maintained a quantitative stabilization though it is gradually differentiate after localization.

Some locally advanced tumors involving multiple abdominal organs without distal metastasis may cause digestive tract obstruction.Some of these patients can achieve long-term survival after Ro resection,others can obtain digestive tract obstruction relief,pain anesis and survival prolongation after R1 resection combined with postoperative comprehensive treatment.In this review,the efficacy of complex pancreaticoduodenectomy combined with organs resection was introduced based on reviewing the literatures.After the surgery,some patients achieved R0 resection and long-term survival,and the quality of life was significantly improved in some patients.

[Objective]To elucidate the experience of treating radial nerve injury with external treatment based on"treatment for flaccid paralysis aiming at Yangming meridian".[Methods]Based on the symptoms and manifestations of radial nerve injury,the etiology and pathogenesis were analyzed,and the connotation of"treatment for flaccid paralysis aiming at Yangming meridian"was deeply analyzed.The strategies of treating radial nerve injury with external treatment were expounded with the guidance of classical articles,and a typical medical case was presented for demonstration.[Results]The causes of radial nerve injury are nothing more than deficiency and excess.The deficiency symptoms are deficiency of the spleen and stomach,deficiency of the liver and kidney,and the excess symptoms include dampness,heat,blood stasis,phlegm turbidity block,and it is easy to be mixed with deficiency and excess."Treatment for flaccid paralysis aiming at Yangming meridian"not only focuses on the spleen and stomach,but also not blindly tonifying the spleen and stomach,but paying attention to regulating the spleen and stomach as the core,distinguishing deficiency and excess,taking into account the five viscera,and clearing and tonifying Qi and blood as the fundamental.The external treatment strategy under the guidance of"treatment for flaccid paralysis aiming at Yangming meridian"can be summarized as"use acupuncture treatment,and focus on selecting Yangming""use acupressure to promote Qi and blood""external use of traditional Chinese medicine to promote blood circulation and remove meridian obstruction""rehabilitation exercise,supplemented by physiotherapy"and"combining Chinese and western,and get results quickly",clinical flexible cooperation of many means can harvest significant results.In the medical case cited,the patient suffered from the radial nerve injury due to a car accident,and identified as the flaccidity syndrome of blood stasis block.The treatment was based on the principle of promoting blood circulation and removing blood stasis,clearing the channels and relieving pain,and the comprehensive use of acupuncture,massage and traditional Chinese medicine fumigation and washing was used to treat the patient's wrist freely for one month,with remarkable results.[Conclusion]"Treatment for flaccid paralysis aiming at Yangming meridian"is not only tonifying the deficiency of the spleen and stomach,but focusing on dredging Yangming Qi and blood,and producing normally.The theory guides the thinking of treating radial nerve injury from the angle of promoting blood circulation and removing blood stasis,attaches importance to the advantages of acupuncture,massage and traditional Chinese medicine fumigation and washing.

Fullerene is an effective free radical scavenger and antioxidant. The fullerene derivatives obtained by chemical modification of fullerene have good water solubility and biological activities. Fullerene and its derivatives have many advantages in cell protection and antioxidant properties, antibacterial activity, antiviral activity, photodynamic activity, drug delivery and anti-tumor activities, playing an important role in the field of medicine. In recent years, great progress has been made in this field. In this review, we summarized the latest research progress and applications of fullerene and its derivatives in medicine field at home and abroad from four aspects of regulating tumor microenvironment, drug delivery, photodynamic therapy and anti-oxidative stress. At last, the future development and application of fullerene and its derivatives in the domain of medicine are prospected.
10.11665/j.issn.1000-5048.20180202

Objective To investigate effect of modified yinqiao powdeRon the expression of gene profiling in monkey with hand-foot-mouth disease infected by CA16 cells of genomics.Methods Thirty SPF Ganges riveRyoung monkey were randomly divided into treatment group (n=15) and control group (n=15).The monkeys in treatment group were treated with yinqiao powder,while the monkeys in control group were treated nothing.AfteR7 days,sample gene expression profiles were analyzed by PCA.The gene expression patterns between two groups were studied.Results Totally 894 differentially expressed genes were screened by gene expression profiles,which were significantly clustered into 2 majoRtypes.Differential expression of genes involved in the enrichment of moleculaRfunction,including many inflammatory chemokines expression,cell communication,cell cycle,regulation of the immune system process,transcription and metabolism process of biological process.Conclusion There are significant differences in the gene expression profile of ovarian granulosa cells between the two groups.The biological processes involved in the differential expression of genes are related to the effects of yinqiao powdeRon CA16 induced inflammatory chemotaxis and immune system response.