Purpose To investigate the expression level and the role of TUSC7 in human cutaneous malignant melanoma (CMM).Methods Quantitative real time-PCR (qRT-PCR) assay was performed to detect the expression of TUSC7 in 60 cases of CMM tissues,20 cases of benign nevus,4 CMM cell lines,and one normal human epidermal melanocytes.Then overexpression of TUSC7 was performed and its role in tumor progression was explored.Results TUSC7 expression was significantly downregulated in primary CMM tissues (n =60) compared to benign nevi (n =20),which were significantly downregulated in all the four melanoma cell lines,especially in A375 cells,compared with the normal melanocytes cells (all P ＜ 0.05).In comparison with the A375 cells transfected with the empty plasmid,those transfected with pcDNA-TUSC7 showed an obvious decrease in the proliferation and colony formation activity,while increase in the apoptosis rate (all P ＜ 0.05).Conclusion Our results suggested that the dysregulation of TUSC7 may play an important role in the CMM progression.

Objective:To establish a three-dimensional visualization model of craniofacial hard and soft tissues with data based on CT. It could be used in clinic as diagnosis and operation simulation. Methods:Original data of patients' craniofacial hard and soft tissues by CT scanning was transferred into the system. After 2-D and 3-D image preprocessing, advanced Marching Cubes Algorithm (Marching Tetrahedron Algorithm) was used for surface fitting;3-D volume rendering was accomplished by footprint method. Results: The whole craniofacial frame and its surface could be observed clearly in reconstructed 3-D Model. Furthermore, the 3-D Model could be seen from any sight angle and sectioned in any direction and place. Doctors could observe the characters of craniofacial deformity for more details. Conclusion: The problems of X-ray reflection of metal brackets, control of X-ray dosage and time spending in 3-dimensional visualization model reconstruction were solved. This model could be used in clinic for diagnosis and operation simulation.

Objective: To investigate the effects of silencing nuclear division cycle 80 (NDC 80) gene expression on the proliferation, migration and invasion abilities of human gallbladder carcinoma GBC-SD and NOZ cells. Methods: Real-time fluorescent quantitative PCR and Western blotting were used to detect the expressions of NDC80 mRNA and protein in gallbladder carcinoma GBC-SD and NOZ cells after the transfection of NDC80 siRNA, respectively. After NDC80 gene silencing, the proliferation of GBC-SD and NOZ cells were detected by CCK-8 test and clone formation test, respectively. Transwell chamber assay was used to detect the migration and invasion abilities of gallbladder carcinoma cells after NDC80 gene silencing. Results: The NDC80 siRNA (si-NDC80 group) and the negative control (si-NC group) were successfully transfected into gallbladder carcinoma GBC-SD and NOZ cells, respectively. The expression levels of NDC80 mRNA and proteins in GBC-SD and NOZ cells transfected with si-NDC80 were significantly lower than those in si-NC group (all P < 0.001). Compared with the si-NC group, si-NDC80 transfection reduced the proliferation and clone formation abilities of GBC-SD and NOZ cells (all P < 0.001), and the migration and invasion abilities of GBC-SD and NOZ cells transfected with si-NDC80 were significantly decreased (all P < 0.001). Conclusion: Silencing the expression of NDC80 gene can inhibit the proliferation, migration and invasion of human gallbladder carcinoma cells, suggesting that NDC80 plays an important role in the occurrence and development of gallbladder carcinoma.

Cholecystectomy is currently the most important surgical treatment for gallbladder disease, however, the complications of cholecystectomy, especially the occurrence of intestinal tumors has gradually aroused attention of scholars. In recent years, scholars have found that bile acid metabolism changes significantly after cholecystectomy, which may be the main cause of colorectal cancer induction, but the specific mechanism of colorectal cancer induced by cholecystectomy has not been clarified. In this article, the changes of bile acid metabolism after cholecystectomy (the size and composition of bile acid pool) and the mechanism of colorectal cancer induction after cholecystectomy were summarized and discussed based on the research progress at home and abroad, aiming at providing theoretical basis for the exploration of the prevention and treatment of colorectal cancer.

After the promulgation and implementation of the Vaccine Administration Law of the People’s Republic of China， the compensation of suspected adverse reactions in China is to be reformed and innovated. There have been attempts at compensation through government finance and insurance， but there has been no precedent for a fund of vaccine-related compensation in China， which means that this could be a new method of solving disputes of compensation for vaccine-related incidences and enhancing public confidence in vaccination. It is suggested that under the current system， we can select a province as a pilot to explore the fund compensation mechanism. The fund comes from special financial allocation， special taxation of vaccine enterprises， fund investment income， charitable donation and other channels. Through a special fund management organization， the independent identification and compensation process can be realized， so as to shorten the current compensation procedure， improve the amount of compensation， ultimately protect the interests of all parties， and promote the steady development of vaccination.