Objective: Effects of cholesterol oxides (Ch-Ox) on plasma prostacyclin (PGI 2) and endothelin (ET) levels of rats in different selenium (Se) status were investigated. Methods: Weaning male Wistar rats were fed Se deficient diet (containing Se 0.038 mg/kg) and stock diet (Se 0.326 mg/kg, control group) respectively, for 13 weeks. One of Ch-Ox, cholestane-3 beta, 5 alpha, 6 beta-triol (3-triol) was injected into the rats through tail vein.24 h after treatment, the plasma PGI 2, thromboxane A 2 (TXA 2) and ET of animals were determined by radioimmunoassay. Results: Blood Se content, glutathione peroxidase activity and plasma PGI 2 levels of Se deficient rats were significantly lower than that of the control group (P

Intracerebral hemorrhage is the most important complication after intravenous thrombolytic therapy for acute ischemic stroke.It often occurs inside the infarct focus,and a considerable proportion occurs outside the infarct focus (i.e.remote intracerebral hemorrhage).Remote intracerebral hemorrhage has different risk factors,pathophysiological mechanisms and prognosis from intralesional hemorrhage.In recent years,more and more research has begun to focus on remote intracerebral hemorrhage.This article reviews the diagnostic typing,clinical features,risk factors and potential pathophysiological mechanisms of remote intracerebral hemorrhage after intravenous thrombolysis in acute ischemic stroke.

Amino Acid Sequence ; Autophagy ; Binding Sites ; Genomics ; Molecular Sequence Data ; Phosphorylation ; Saccharomyces cerevisiae Proteins ; chemistry ; genetics ; metabolism

Targeted protein degradation(TPD)has rapidly emerged as a therapeutic modality to eliminate previously undruggable proteins by repurposing the cell's endogenous protein degrada-tion machinery.However,the susceptibility of proteins for targeting by TPD approaches,termed"degradability",is largely unknown.Here,we developed a machine learning model,model-free anal-ysis of protein degradability(MAPD),to predict degradability from features intrinsic to protein tar-gets.MAPD shows accurate performance in predicting kinases that are degradable by TPD compounds[with an area under the precision-recall curve(AUPRC)of 0.759 and an area under the receiver operating characteristic curve(AUROC)of 0.775]and is likely generalizable to inde-pendent non-kinase proteins.We found five features with statistical significance to achieve optimal prediction,with ubiquitination potential being the most predictive.By structural modeling,we found that E2-accessible ubiquitination sites,but not lysine residues in general,are particularly associated with kinase degradability.Finally,we extended MAPD predictions to the entire proteome to find 964 disease-causing proteins(including proteins encoded by 278 cancer genes)that may be tractable to TPD drug development.

Ubiquitination, an essential post-transcriptional modification (PTM), plays a vital role in nearly every biological process, including development and growth. Despite its functions in plant reproductive development, its targets in rice panicles remain unclear. In this study, we used proteome-wide profiling of lysine ubiquitination in rice (O. sativa ssp. indica) young panicles. We created the largest ubiquitinome dataset in rice to date, identifying 1638 lysine ubiquitination sites on 916 unique proteins. We detected three conserved ubiquitination motifs, noting that acidic glutamic acid (E) and aspartic acid (D) were most frequently present around ubiquitinated lysine. Enrichment analysis of Gene Ontology (GO) annotations and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of these ubiquitinated proteins revealed that ubiquitination plays an important role in fundamental cellular processes in rice young panicles. Interestingly, enrichment analysis of protein domains indicated that ubiquitination was enriched on a variety of receptor-like kinases and cytoplasmic tyrosine and serine-threonine kinases. Furthermore, we analyzed the crosstalk between ubiquitination, acetylation, and succinylation, and constructed a potential protein interaction network within our rice ubiquitinome. Moreover, we identified ubiquitinated proteins related to pollen and grain development, indicating that ubiquitination may play a critical role in the physiological functions in young panicles. Taken together, we reported the most comprehensive lysine ubiquitinome in rice so far, and used it to reveal the functional role of lysine ubiquitination in rice young panicles.
Acetylation ; Lysine/metabolism* ; Oryza/metabolism* ; Plant Proteins/metabolism* ; Protein Interaction Maps ; Protein Processing, Post-Translational ; Proteome/metabolism* ; Ubiquitin/metabolism* ; Ubiquitination