Objective To investigate the human papillomavirus (HPV) infection situation among female patients and its subtypes distribution rule for providing the measures of diagnosis and prevention .Methods The nucleic acid hybridization was adopted to de‐tect HPV subtypes of cervical exfoliated cells in 12 422 female outpatients and inpatients in a hospital .The HPV infection situation among different patients were analyzed .Results The HPV infection rate was 17 .0584% ,the HPV infection rate had statistical difference among different age groups (P<0 .01) .Totally 20 subtypes of HPV were identified .HPV16 ,HPV52 and HPV58 were the most prevalent high‐risk subtypes .Conclusion The HPV subtype detection can be used in the screening and condition judgment of cervical cancer .The key for the HPV positive population is early diagnosis and early treatment for blocking the HPV persistent infection and preventing the cervical cancer occurrence .

Objective To investigate the pathogenesis of small cell lung cancer ( SCLC) and to ex-plore the expression of cell cycle related kinase ( CCRK) in SCLC and its clinical significance.Methods Reverse transcription polymerase chain reaction ( RT-PCR) and Western blot were performed to examine ex-pression of CCRK in SCLC and normal tissues.Results The expressions of gene [(0.51 ±0.11)IU/L] and protein [(0.61 ±0.13)IU/L] of CCRK in SCLC tissues were significantly higher than normal tissues [(0.30 ±0.08)IU/L, (0.34 ±0.09)IU/L] ( P <0.05).The expression of CCRK was closely correlated with the clinical curative effect ( P <0.05 ) rather than the clinical stages ( P >0.05 ) .Conclusions The expressions of gene and protein of CCRK in SCLC tissues were significantly higher than normal tissues. CCRK promoted the occurrence and progress of SCLC.Chem can restrain effectually the excessive expres-sion of CCRK.The expressions of gene and protein of CCRK in the different clinical curative effect group had significant difference.

The laboratory teaching of bacteriological analysis is very important for training practical analysis professionals.For better quality of laboratory teaching,in this paper,we practice a series of reform facilitating the lab teaching of bacteriological analysis,which is involved in the exploration and study of new curriculum system and teaching evaluation.The educational practices create effective teaching and learning environment and better performance.