By means of retrograde HRP tracing method, the innervation of the left half of cervical portion of oesophagus in cats was investigated in this study. The HRP-labelled cells were found bilaterally in the retrofacial nuclei, nuclei supraspinalis, nuclei dorsomedialis and ipsilaterally in the dorsal motor nucleus of the vagus nerve, solitary nucleus, nucleus ambiguus, intermediate zone, nucleus retroambiguus and spinal nucleus of the accessory nerve. The distribution of the labelled cells in the dorsal motor nucleus of vagus nerve showed certain localization characteristics. They were more numerous at the levels above and below the obex. The labelled sympathetic preganglionic neurons with long axons appeared in T_1-T_3 spinal segments at the injected side (88.96%) and mainly in T_2(66.45%). The majority of labelled cells were located in the intermediolateral nucleus(95.02%); and the rest were in the lateral funiculus, intercalated nucleus and dorsolateral nucleus of the ventral horn, there were two of preganglionic neurons (i.e. parasympathetic and sympathetic) innervating the oesophagus. The postganglionic neurons were mainly located in the stellate ganglion(61.99%), anterior and middle cervical ganglia, and T_2-T_5 ganglia of the sympathetic trunk, bilaterally. There were some labelled cells in the nodose ganglion and the C_1 to T_5 spinal ganglia(mainly in C_6 and T_2). The ratio between the labelled cells in the nodose ganglion and the spinal ganglia is 2.6:1, which means that the afferent fibres ran predominantly via vagus nerve.

Objective:Through a study on knowledge, attitudes, and practice of tobacco control of medical staff in Gansu Province, the paper aims to analyze the issues of participation, community tobacco control leadership and other aspects that impact the creation of a smoke-free healthcare system in order to provide a basis for the formulation of policies to improve the health professional mechanism of tobacco control. Methods:The stratified cluster sampling method was adopted to select 34 companies, accounting for a total of 805 medical staff as the study population in all kinds of health institutions. A self-designed questionnaire was adopted to investigate their knowledge, attitudes, and practice via on-site self-administered surveys. Results:the medical staff lacks knowledge as to the depth and breadth of damage from the use of tobacco. Although their attitude towards tobacco control is positive, the comprehensive to-bacco control service capabilities are insufficient. Logistic regression analysis showed that tobacco control capabilities of medical staff is related to their attitude toward smoking and whether or not they discourage smoking or asking about smoking . Conclusion:The key to the creation of a smoke-free health system to lead the community in tobacco control is the targeted tobacco cessation knowledge and skills training of medical staff to reduce smoking rates among medical staff and enhance the awareness of administrative staff.

Previous studies proposed that the synergistic effect of fibroblast growth factor-21 (FGF-21) and insulin may be due to the improvement of insulin sensitivity by FGF-21. However, there is no experimental evidence to support this. This study was designed to elucidate the mechanism of synergistic effect of FGF-21 and insulin in the regulation of glucose metabolism. The synergistic effect of FGF-21 and insulin on regulating glucose metabolism was demonstrated by investigating the glucose absorption rate by insulin resistance HepG2 cell model and the blood glucose chances in type 2 diabetic db/db mice after treatments with different concentrations of FGF-21 or/and insulin; The synergistic metabolism was revealed through detecting GLUT1 and GLUT4 transcription levels in the liver by real-time PCR method. The experimental results showed that FGF-21 and insulin have a synergistic effect on the regulation of glucose metabolism. The results of real-time PCR showed that the effective dose of FGF-21 could up-regulate the transcription level of GLUT1 in a dose-dependent manner, but had no effect on the transcription level of GLUT4. Insulin (4 u) alone could up-regulate the transcription level of GLUT4, yet had no effect on that of GLUT1. Ineffective dose 0.1 mg kg(-1) FGF-21 alone could not change the transcription level of GLUT1 or GLUT4. However, when the ineffective dose 0.1 mg x kg(-1) FGF-21 was used in combination with insulin (4 u) significantly increased the transcription levels of both GLUT1 and GLUT4, the transcription level of GLUT1 was similar to that treated with 5 time concentration of FGF-21 alone; the transcription level of GLUT4 is higher than that treated with insulin (4 u) alone. In summary, in the presence of FGF-21, insulin increases the sensitivity of FGF-21 through enhancing GLUT1 transcription. Vice versa, FGF-21 increases the sensitivity of insulin by stimulating GLUT4 transcription in the presence of insulin. FGF-21 and insulin exert a synergistic effect on glucose metabolism through mutual sensitization.

Objective:To explore prognostic lncRNA related with fatty acid metabolism,immune response and drug sensitivity in hepatocellular carcinoma(HCC).Methods:Bioinformatics was used to obtain HCC gene data of TCGA database,most prognostic lncRNA were screened out,and prognostic model was constructed,whose accuracy was verified by ROC curve and nomogram.GO and KEGG were used for biological analysis of lncRNA.GSVA and TMB were used to analyze immune status and mutant genes of HCC patients.TIDE score was used to analyze benefit of immunotherapy in HCC patients to identify potentially effective drugs for HCC.Results:Four lncRNAs(AC074117.1,TMCC1-AS1,LINC01063,MKLN1-AS)related to fatty acid metabolism were identified.K-M curve and ROC curve confirmed that stage and risk score could evaluate prognosis of HCC patients.Nomogram confirmed accuracy of the model prediction;GO and KEGG analysis confirmed abundant biological functions of lncRNA related to fatty acid metabolism.GSVA analysis showed that plasma cells,B cells,neutrophils,NK cells and type Ⅱ-IFN response were significantly different between two risk groups.Patients with low TMB and low risk had the best prognosis.TIDE score of high-risk group was significantly higher than that of low-risk group.Five anticancer drugs more suitable for high-risk population were screened out,namely cyclopamine,rapamy-cin,Proteasome inhibitor,paclitaxel and sunitinib.Conclusion:Prediction model constructed by 4 fatty acid metabolism-related lncRNAs helps to evaluate prognosis of HCC and may provide insights for clinical application and immunotherapy of HCC patients.

Objective: The present study evaluated the clinical effects of Invisalign-aided molar distalization in the treatment of mild or moderate crowding in anterior teeth. Methods: Eleven adults with class Ⅱ dental malocclusion and a class Ⅰ skeletal pattern were selected as subjects. The patients’ molar occlusion did not exhibit an end-to-end relationship. Subjects were selected for straight profile, mild or moderate crowding in maxillary teeth and normal or mild crowding in mandibular teeth. Nonextraction and Invisalign-aided molar distalization were planned for treatment. Model measurement and cephalometric analysis were performed before and after treatment. A paired t test was used for the statistical analysis. Results: The crowding and class Ⅱ molar relationship were corrected in all 11 patients. The upper first molars were moved distally by 2.32 mm (t = 3.315, P < 0.01) and were inclined distally by 3.35° (t = 3.959, P < 0.01) on average. The central incisors were protruded by 1.72 ° (t = 3.274, P < 0.01) on average. The buccal movement of the upper first molars was 1.32 mm (t = 2.461, P < 0.05) on average. The above differences were statistically significant. Conclusion Upper molar distalization can be achieved using a class Ⅱ elastic-aided Invisalign technique. The end-to-end molar occlusion can be corrected, and front teeth with mild or moderate crowding can be aligned using our treatment protocol.

Traditional surgical resection, radiotherapy, and chemotherapy still play a dominant role in the treatment of liver cancer; however, their application in liver cancer patients is often limited by the toxic and side effects, unstable efficacy, and unclear targets of chemotherapeutic drugs. Therefore, in order to improve the efficacy of drugs in the treatment of liver cancer, nanomedicine, which has been developed in the biomedical field in recent years, has attracted more and more attention. Nano-drug delivery system has been gradually applied in clinical research for its advantages of low toxicity, wide bioavailability, controllable drug release, and good stability. This article focuses on the latest research advances in nano-drug delivery system in the treatment of liver cancer.

[Objective] To evaluate the risk of bacterial contamination of platelets apheresis and improve the clinical diagnosis rate of transfusion-transmitted bacterial infections. [Methods] A retrospective analysis was conducted on 11 cases involving bacterial contamination detected in apheresis platelets during quality inspections at our center from 2021 to 2023, as well as cases of transfusion-transmitted bacterial infection (TTBI) caused by platelet transfusion. The return of positive platelet bacteria test results and clinical transfusion adverse reactions were statistically analyzed. [Results] There were 9 donors with bacteria-contaminated platelets, of which 3 were reported as clinical transfusion reaction, 4 were detected by quality sampling, and 2 were identified by appearance detection before transfusion. The bacterial contamination rate of platelets was about 0.08% (9/10 762). The contaminated platelets were involved in 11 cases of TTBI, with an incidence of TTBI of approximately 0.05% (11/21 916). Only 3 cases of transfusion reactions were clinically reported, while the rest were case tracking with positive results of platelet bacterial test from quality sampling. The clinical return rate of TTBI was 27.27% (3/11), with an average reporting time of 8.12 hours after the occurrence of transfusion reactions. The majority of the contaminated platelets were detected at the end stage of storage, with 55.56% (5/9) of platelets collected on the 4th day after collection. Partial contaminated platelets were identified through quality sampling, with a positive rate of 2.78% (4/144). [Conclusion] The platelet bacterial contamination rate is high, but with low clinical return rate. It is recommended to conduct routine platelet bacterial monitoring and improve clinical diagnostic level.