Objective To establish a method for fingerprint analysis of amino acids from honey by high performance liquid chromatography ( HPLC). Methods Amino acids of honey were concentrated by 732 cation exchange resin, and then were treated by pre-column derivatization with phenyl isothiocyanate, with praline as control peak. The chromatography was performed on a Waters Symmetry C18 ( 250 mm × 4.6 mm × 5 μm) column, with acetonitrile ∶ water (4∶1) as mobile phase A and 30 mmol/L sodium acetate ∶ acetonitrile (355∶15, acetic acid adjusting pH value to be 6.5) as mobile phase B by gradient elution. The detection wave length was set at 254 nm. The flow rate was 1.0 mL/min. The column temperature was 40℃, and the injection volume was 5μL. Results Sixteen common peaks were shown in the fingerprint of 15 batches of honey samples. The similarity for 15 batches of honey samples was in the range of 0.910 ~ 0.996 . Conclusion The fingerprint detection method is simple, practical, reproducible and specific, and can provide certain reference for quality control of honey.

This investigation was directed to the physiological load intensity in the curricula of track and field studies offered in 8 general colleges. We adopted the sampling survey method and monitored the heart rate of 80 first-year students who were from different grades in 8 colleges and were attending the track and field class. The data collected from these male student show: (1) The pre-exercise heart rate is 80 per minute; the first inflection point appears at 28 minutes after exercise; the heart rate is 132 per minute and the slope is -0.031. (2) The data of exercise load at mobilization stage and at fatigue stage are 90.09 and 104.126 kUw respectively; the data of lasting time are both 28 minutes, and the average heart rates are 133 per minute and 140 per minute respectively. The exercise load is 84.231 kUw at stable stage. (3) When the heart rates are 120-140 per minute and 140-150 per minute respectively, the ratios of load are 32.786% and 31.316% respectively. When the heart rate is 120-150 per minute, the ratio of load is 64.102%. But we are surprised to find that when the heart rate is 150-160 per minute, the ratio of load is only 17.639%. In the analysis of data from this investigation we have noticed that the physiological load intensity is in accord with the changing rule of body function. In order to further adjust the training plan and arrange the load of exercise rationally and promptly, we, the teachers, should pay adequate attention to the heart rate monitoring data on students.
Curriculum ; Female ; Heart Rate ; Humans ; Male ; Physical Education and Training ; Students ; Track and Field ; Universities ; Weight-Bearing ; physiology ; Young Adult