1.Effects comparison of gemstone energy spectrum CT atomic number method and infrared spectroscopy for analyzing composition of urinary calculus
Jiali ZHU ; Yi WANG ; Zhiwei LI ; Qun QIN ; Fuying QIU ; Zexia GUO ; Zeqin YAO ; Houzhou LUO ; Zhenqing HUO ; Bing WENCONG ; Liang LIU
Chongqing Medicine 2017;46(33):4662-4663,4666
Objective To investigate the effects of gemstone energy spectrum CT atomic number method and infrared spec-troscopy for analyzing the composition of urinary calculi and to compare their values in qualitative diagnosis of urinary calculi .Meth-ods Two hundreds and sixty cases of urinary tract stones were performed the gemstone spectrum CT urinary scanning and the stone composition was identified by atomic number method .After removing stone ,the stone composition analyzed by infrared spec-troscopy served as the gold standard .Then the consistency identified by the two methods was analyzed .Results The Kappa consis-tency test results showed that the two kinds of method for identifying stone type had good consistency (Kappa=0 .787 ,P<0 .01) . The paired chi square test results showed that the difference of the two methods for identifying the stone type had no statistical sig-nificance(χ2 =6 .581 ,P=0 .254) .The stone crystal composition types measured by gemstone energy spectrum CT atomic number method were less than those measured by infrared spectroscopy .The precise quantification of the stones with different crystal struc-tures was not as accurate as that of infrared spectroscopy (calcium oxalate monohydrate and calcium oxalate dihydrate ) .Conclusion The two methods for analyzing theurinary stone composition all have clinical significance ,the stone analysis method should be se-lected according to the actual situation .
2.Spindle assembly checkpoint complex-related genes TTK and MAD2L1 are over-expressed in lung adenocarcinoma: a big data and bioinformatics analysis.
Zhu LIU ; Zeqin GUO ; Lili LONG ; Yanpei ZHANG ; Yuwen LU ; Dehua WU ; Zhongyi DONG
Journal of Southern Medical University 2020;40(10):1422-1431
OBJECTIVE:
To screen the key genes related to the prognosis of lung adenocarcinoma through big data analysis and explore their clinical value and potential mechanism.
METHODS:
We analyzed GSE18842, GSE27262, and GSE33532 gene expression profile data obtained from the Gene Expression Omnibus (GEO). Bioinformatics methods were used to screen the differentially expressed genes in lung adenocarcinoma tissues and KEGG and GO enrichment analysis was performed, followed by PPI interaction network analysis, module analysis, differential expression analysis, and prognosis analysis. The expressions of MAD2L1 and TTK by immunohistochemistry were verified in 35 non-small cell lung cancer specimens and paired adjacent tissues.
RESULTS:
We identified a total of 256 genes that showed significant differential expressions in lung adenocarcinoma, including 66 up-regulated and 190 down-regulated genes. Thirty-two up-regulated core genes were screened by functional analysis, and among them 29 were shown to significantly correlate with a poor prognosis of patients with lung adenocarcinoma. All the 29 genes were highly expressed in lung adenocarcinoma tissues compared with normal lung tissues and were mainly enriched in cell cycle pathways. Seven of these key genes were closely related to the spindle assembly checkpoint (SAC) complex and responsible for regulating cell behavior in G2/M phase. We selected SAC-related proteins TTK and MAD2L1 to test their expressions in clinical tumor samples, and detected their overexpression in lung adenocarcinoma tissues as compared with the adjacent tissues.
CONCLUSIONS
Seven SAC complex-related genes, including TTK and MAD2L1, are overexpressed in lung adenocarcinoma tissues with close correlation with the prognosis of the patients.
Adenocarcinoma of Lung/genetics*
;
Big Data
;
Cell Cycle Proteins/genetics*
;
Computational Biology
;
Gene Expression Profiling
;
Gene Expression Regulation, Neoplastic
;
Humans
;
Lung Neoplasms/genetics*
;
M Phase Cell Cycle Checkpoints
;
Mad2 Proteins/genetics*
;
Protein-Serine-Threonine Kinases/genetics*
;
Protein-Tyrosine Kinases/genetics*
3. Research of TLD calibrated in different standard X-ray RQR radiation field in terms of eye lens dose H p(3)
Wenyan LI ; Guiying ZHANG ; Zhichao WANG ; Zeqin GUO ; Mengxiao KANG ; Chuanye LIU ; Wentao SHENG ; Yue ZENG ; Xi LUO ; Linlin YUE
Chinese Journal of Radiological Medicine and Protection 2020;40(1):42-46
Objective:
To compare the calibration result of standard X-ray RQR radiation field between SSDL (NIRP) and CEA LIST LNHB(France), and to explore the feasibility of calibrating
4.Discussion of Hp(3) calibration with two thermoluminescent dosimeters in the same standard X-ray RQR radiation field
Wenyan LI ; Guiying ZHANG ; Lantao LIU ; Dongsheng NIU ; Zeqin GUO ; Zhichao WANG ; Hua TUO ; Heyan WU ; Tingting XIA ; Nini CHU ; Jichuan LAI ; Jiaojiao CHEN
Chinese Journal of Radiological Health 2024;33(3):318-322
Objective To compare Hp(3) calibration with a homemade (A) thermoluminescent dosimeter (TLD) and an imported (B) TLD in a standard X-ray RQR radiation field, to explore the different responses of A and B, and to provide foundation for the calibration of Hp(3). Methods A column mode was selected. Hp(3) calibration was performed using A and B in a standard X-ray RQR radiation field in the Secondary Standard Dosimetry Laboratory, National Institute for Radiological Protection, China Center for Disease Control and Prevention. Angle response, energy response, and linear response were calibrated with RQR4 (60 kV), RQR7 (90 kV), and RQR9 (120 kV), respectively. Results In terms of angle response, the calibration results of A were relatively high, while the calibration results of B were relatively low. In terms of energy response, the calibration results showed a similar pattern to angle response. In terms of linear response, the calibration results of both A and B were satisfactory. Conclusion Both A and B can be used for normal calibration of Hp(3) in a standard X-ray RQR radiation field. However, in actual monitoring, attention should be paid to the energy and angle response values of TLDs.