1.Effects of motilin on potassium and calcium currents of rat's proximal colon smooth muscle cells
Zengyou WU ; Hesheng LUO ; Chengbai LIANG ; Ying LIU ; Hong XIA
Chinese Journal of Digestion 2012;32(7):445-449
Objective To investigate the effects of motilin on the voltage dependent potassium channel and L-type calcium channel currents in rat proximal colon smooth muscle cells (PCSM) and to explore its mechanism in increasing colonic motility.Methods PCSM were isolated by collagenase.The voltage dependent potassium channel transit outward current (IKA ) and delayed rectifier current (IKdr) and L-type calcium currents (ICa(L)) were measured by whole cell patch clamp technique.Groups were analyzed by paired t-test.Results There was no significant effect of motilin on IKA and IKdr.L-type calcium channel was dose-dependently activated by motilin from 0.5 × 105 mmol/L to 10.0 ×10-5 mmol/L.At 6 × 10-5 mmol/L motilin and under - 10,0 and 10 mV stimulating voltage,maximum current density increased by 154.61%,62.69% and 21.02% respectively and activation kinetics curve obviously left shifted.Half activation voltage decreased from (2.740±1.211) mV prior administration to ( - 25.290 ± 0.614) mV (t =8.534,P =0.007 ) and there was no significant difference in slope factor. Conclusions Motilin increases colonic smooth muscle contraction by promoting calcium influx. However the frequency of colonic smooth muscle contraction could not change with frequency of equilibrium potential and action potential of colonic smooth muscle.
2. The mediation mechanism of coronary artery lesions in both male and female patients with Kawasaki disease
Yihan ZHANG ; Rongzhou WU ; Junyong HU ; Zengyou JIN ; Zehao YE ; Huixian QIU ; Maoping CHU ; Hongying SHI
Chinese Journal of Epidemiology 2019;40(12):1634-1638
Objective:
To explore the mediation mechanism of coronary artery lesion among both male and female Kawasaki disease (KD) children.
Methods:
Children with KD that hospitalized in the Wenzhou Medical University affiliated Yuying Children’s Hospital from January 2009 to December 2014, were included in this study. Differences on demographical characteristics, clinical manifestations, laboratory indicators, regimen and time of treatment, results from pre/post echocardiography and treatment between male and female patients, were compared. The independent effect of gender on the risk of coronary artery lesions (CAL) was evaluated, and the mediating effect of BMI, visiting time and KD type on the association between gender and CAL were also studied.
Results:
The average BMI level of male patients was higher than that of female patients. The difference was statistically significant (