Objective To explore the role of Extracellular signal-regulated kinase(ERK)signal transduction pathway in the mechanism of liver fibrosis via investigating the expression of ERK1 in fibrotic liver tissue of human.Methods SABC immunohistochemistry was used to detect the expression of ERK1 in 32 liver fibrosis and 12 normal liver tissues from Jan.2001 to Dec.2003.Results The expression of ERK1 in liver fibrotic tissues were found chiefly in hepatic stellate cells(HSC),and it presented with significantly progressive tendency for the expression of ERK1 in normal and liver fibrosis cirrhosis tissues(P

Objective To explore the feasibility of laparoscopic Ladd’s procedure and its indications for intestinal malrotation accompanying midgut volvulus. Methods Laparoscopic Ladd’s procedure was performed in 15 children from July 2002 to March 2006. The procedure was performed using three trocars. Under laparoscopic visualization, the midgut volvulus was untwisted by grasping and pulling the intestine, the Ladd’s band was divided and broadened, the duodenum and the small intestine were mobilized, and finally an appendectomy was performed through an abdominal wall port. Results Laparoscopic Ladd’s procedure was completed successfully in the 15 children. Of them, 1 patient with duodenal web, 1 patient with paraduodenal hernia, and 1 patient with ectopic pancreas also had a concomitant procedure. The operative time was 45~150 min (mean, 75 min). The patients began to take food on 1~3 postoperative day. There was no surgical complications. The length of postoperative hospital stay ranged 4~6 days (mean, 5 days). Follow-up observations in 12 patients for 1~42 months (mean, 21 months) showed normal development and free of symptoms.Conclusions Laparoscopic Ladd’s procedure is a safe and effective technique. It can be performed in neonates and applicable subacute midgut volvulus, but may be unadvisable for acute volvulus with abdominal distention.

Objective To investigate the pathological changes of gastric antrum mucosa after Helicobacter pylori eradication.Methods Total 180 cases who suffered from epigastralgia and took the endoscopic examination were randomly divided into two groups,one was Hp eradication group which included 98 cases and given anti-Hp medication treatment,and the other was control group which included 82 cases and given an expectant treatment.At the end of the study,they took reexamination by gastroscope and tests on Hp by Giemsa dyeing & rapid urase detection and on gastric antrum pathological changes by HE dyeing.Results In treatment group,atrophic gastritis as well as intestinal metaplasia decreased significantly after Helicobacter pylori eradication,but in control group,no change of atrophic gastritis was found while intestinal metaplasia aggravated.Conclusion The eradication of Helicobacter pylori is able to decrease atrophic gastritis and intestinal metaplasia.

Objective To explore the method, safety, and efficacy of laparoscopic intracorporeal sutured gastrojejunostomy.Methods Three children with gastric outlet obstruction underwent laparoscopic sutured gastrojejunostomy from May to September 2005. Of them, two patients with pyloric stenosis secondary to peptic ulceration received a concomitant highly selective vagotomy. A suitable segment of jejunum was lifted over the transverse colon and apposed to the gastric antrum. A continuous 4/0 suture was conducted at the seromuscular layer making the two organs together. Then the stomach and adjacent jejunum were incised with an ultrasonic scalpel. A side-to-side gastrojejunostomy was performed with full-thickness continuous suture of gastric and jejunal wall followed by anterior interrupted suture of seromuscular layers. Results All the intracorporeal sutured gastrojejunostomies were completed successfully under laparoscope. The operating time was 135, 150, and 180 min, respectively. The postoperative hospital stay was 6 d. There was no surgical complications. Postoperative follow-up at 8, 10, and 12 months, respectively, showed that all patients had normal diet and nutriture. Conclusions Laparoscopic sutured gastrojejunostomy is a safe and feasible technique, with advantages of minimal invasion, rapid recovery, and good cosmetic outcomes.

Objective To summarize the experience of laparoscopic massive splenectomy combined with selective pericardial devascularization using endoligature technique. Methods By using silk ligature and hamonic scapel or the LigaSure, 6 patients with portal hypertension and esophagogastric varices underwent laparoscopic massive splenectomy and selective pericardial devascularization. Results All the operations were completed successfully under laparoscope. The intraoperative blood loss was 80~200 ml (mean, 130 ml). None of the patients required blood transfusion or conversion to open procedure. The operation time was 150~210 min (mean, 190 min). There were no surgical complications. All the patients resumed to normal activities 5 days after operation. Follow-up observations for 3~10 months (mean, 8 months) revealed no recurrent variceal hemorrhage. Conclusions Laparoscopic massive splenectomy combined with selective pericardial devascularization using endoligature technique is a feasible, effective, safe, little hemorrhagic, and minimally invasive procedure for portal hypertension with esophagogastric varices.

Objective To explore the role of connective tissue growth factor(CTGF)in the mechanism of liver fibrosis.Methods Liver fibrosis models of rats were made by subcutaneously injecting with CCl4.On the other hand,32 human liver cirrhosis and 12 normal liver tissues were collected too.Immunohistochemical staining was used to detect the expression of CTGF in these tissues.Results The expression of CTGF in rats after injection with CCl4 was found mainly in hepatic stellate cells(HSC) and hepatocytes are all significantly higher than those in normal rats.Moreover,it presents with progressive tendency for the expression of CTGF in rats respectively at 1st,4th and 8th week after injection of CCl4.The same tendency was found in human normal and fibrotic liver tissues(P

Objective To solve the problem of additional airway resistance from oxygen supply tube and humidity liquid dropping tube in trachea pipe for patients with trachea dissection and intubatton along with the fixation of respiration detector.Methods The device was used in patients with trachea dissection and intubatton and the result is desirable.Conclusion This device solves the problem of additional airway resistance from oxygen supply tube and humidity liquid dropping tube in trachea pipe along with the fixation of respiration detector.It is an indispensable device for patients with trachea dissection and intubatton.

Objective To evaluatethe expression and significance of RUNX3 gene and protein in hepatocellular carcinoma.Method Reverse transcription-polymerase chain resction (RT-PCR) and immunoitistochemistry SP method were used to detect the expressions of RUNX3 gene and protein in 23 cases of hepatocellular carcinoma and their adjacent non-cancerous tissue.Results The expression of RUNX3 gene and protein were significantly reduced in hepatocellular carcinoma tissue (26.1% and 17.4% respectively),which were prominently lower than those in adjacent non-cancerous tissue:91.3% (21/23) and 87.0% (20/23),P＜0.01.Conclusion The lower-expression of RUNX3 gene and protein may play an important role in hepatocellular carcinoma.

RT-PCR and Western blot were used to detect the LATS2 mRNA and protein level in 57 cases of oral squamous cell carcinoma (OSCC)specimens and 12 normal oral mucosa tissues.LATS2 mRNA and protein were expressed in all the normal oral mucosa samples (100%),but only 47.4% (27 /57)and 42.1% (24 /57)in OSCC samples respectively with positive correlation of both(P <0.01),and were respectively correlated with the tumor differentiation degree and lymph node metastasis(P <0.05).

OBJECTIVETo apply single nucleotide polymorphism (SNP) microarray for delineation of small supernumerary marker chromosome (sSMC) in two newborns.

METHODChromosome karyotyping was performed on newborns who were born in Jan. 2013 and Jan. 2014 in Haidian Maternal and Child Health Hospital because of the abnormalities found in pregnancy checkups. SNP microarray analysis was carried out on 2 newborns with de novo sSMCs (one was mos 47,XY, + mar[45]/46,XY[5] and the other was mos 47, XY, + mar [30]/46, XY [20]), which could not be determined by conventional banding techniques. Genomic DNA was extracted from cord blood samples, amplified, tagged and hybridized following the manufacturer' s protocol. Data were collected and analyzed.

RESULTThere was a 78. 6 Mb duplication in chromosome 8 for Newborn A, which was associated with 8p22 duplication syndrome; and a 32. 7 Mb duplication in chromosome 13 for Newborn B, which was not yet reported definitely as pathogenic. The newborn A was identified with agenesis of the corpus callosum, obvious right eyelid drooping, the onset of low muscle tone and mental developmental lag behind their peers, while the newborn B had normal findings on physical and mental evaluation.

CONCLUSIONSNP-array can identify sSMCs of newborns at the DNA level, and can be used as an important supplement to the conventional karyotype analysis, but the pathogenicity of positive outputs need further verification.