Cluster Analysis ; Fuzzy Logic ; Humans ; Mathematics ; Medicine, Chinese Traditional

The metagenomic DNA was extracted from the deep sea sediment with the depth of 900m of Prydz Bay, Antarctic. A lipase gene (lip3) with the size of 948bp was cloned from the metagenomic DNA by PCR with the primers designed. The deduced Lip3 protein was composed of 315 amino acids (AA) with a molecular mass of 34.577 ku. The motifs GFGNS(GXGXS)and G-N-S-M-G(GXSXG)in the AA sequences of Lip3 were found to be conserved in other lipase. They were most conserved sequence among the serine hydrolase and were necessary for the activity. A 35 ku of Lip3 was purified by Ni-NTA chelating sepharose column from the extract of recombinant E.coli Top 10F′ cell harboring a pLLP-OmpA plasmid inserted with lip3. The purified Lip3 was most active at 25℃ and kept 22% of activity at 0℃. Only 10% of activity was retained after it was incubated at 35℃ for 60 min. The optimal pH value for the Lip3 activity was 8.0. The Km value of the enzyme towards p-nitrophenyl palmitate increased with the increasing of assayed temperature. These results indicated that Lip3 was a typical alkaline cold active enzyme.

Excessive fructose intake can induce metabolic syndrome and increase serum uric acid levels.Uric acid levels are high in types of fructose-induced metabolic syndrome and may play a pathogenic role in the progression of these diseases.In this review,we focus on the role of uric acid in fructose-induced metabolic syndromes.

Currently,China lacks enough public health physicians and the quality of them varies much.In order to solve this problem,proving the access to public health physician is necessary.On the basis of literature research,this essay summarizes the existing problems of the access to public health physicians:improper professional education in university,curriculum setting and provision about physician registration.The essay argues that it is a feasible way to improve the access of public health physicians in China by further piloting and perfecting the unified post-graduation standardized training system,implementing curriculum reform to enhance students' practical ability and building network physician registration.

Objective:To explore the significance of multiple detector spiral CT multi-phase enhanced scan in differentiating liver tumors.Methods: A total of 141 patients, during from March 2011 to May 2015 in our hospital, pathologically diagnosed as liver tumors were selected to undergo multiple detector spiral CT multi-phase enhanced scan. The different performances and results in CT plain and enhanced scan for diagnosing liver cancer were observed and the sensitivity of CT multi-phase enhanced scan was analyzed.Results: 93 cases were found in CT plain scan; 141 cases were found in the arterial phase, 112 cases were found in portal venous phase and 108 cases were found in extension period in CT multi-phase enhanced scan. The lesion detection rate of CT plain scan was significantly lower than whichever of CT multi-phase enhanced scan(P<0.05); under CT multi-phase enhanced scan, the majority of lesions were typical imaging and could be diagnosed. Using CT multi-phase enhanced scan, the sensitivity of hemangiomas was 88.9%, abscess was 71.4%, focal nodular hyperplasia was 83.3%, primary liver cancer was 81.6%, and liver metastases was 97.8%; the total accuracy was 88.7%.Conclusion: The multiple detector spiral CT multi-phase enhanced scan can accurately identify liver tumor lesion stages, and it can contribute to further clinical treatment.

This article elaborates on the problem of nursing action accountability,that is,ethical accountability and law accountability.Through two vcrdict examples it gives furthor explanation of nursing action accountability.Therefore,nurses should take the patients' interest into first account and closely bond the internal restraining system-ethical accoantability and the external monitoring system-law accountability together,then they can protect their legitimate right and provide high quality of care for patients.

Objective To investigate the clinical manifestations of cytomegalovirus (CMV) infection in patients with systemic lupus erythematosus (SLE). Methods Data of the consecutive cases of SLE complicated with active CMV infection including clinical manifestations, SLEDAI score, dosage of corticosteroid and immunosuppressants used for treatment,radiological and laboratory examinations were collected and analyzed.Results Among 2221 consecutive patients of SLE, 5.4%(121 cases) were diagnosed to be complicated with active CMV infection. Fever was the most common symptom, followed by serious liver function damage,respiratory symptoms,hematological abnormalities, myocarditis, and encephalopathy, accounted for 81%(98cases), 6.6%(8 cases), 4.1%(5 cases), 3.3%(4 cases), 0.8%(1 case), and 0.8%(1 case)respectively; in addition, 22 (18.2%) cases had no symptom. SLEDAI was higher than 15 in 47.1% cases, and 10-14 in 28.1% cases. 81% of patients were treated with corticosteroid, and 55.4% were treated with immunosuppressants. Ganciclovir was given once the diagnosis of active CMV infection was established. In most of the patients, active CMV infection had been controlled within 14-28 days, except 4 died and 6 gave up the therapy. Conclusion SLE with active CMV infection is common,especially in patients who are treated with corticosteroid and/or immunosuppressants. Clinical manifestations of SLE complicated with active CMV infection are generally nonspecific.In patients with unexplained fever,or liver damage,or lung disease,or active SLE patients who have no symptom but are refractory to the treatment, CMV infection should be suspected and the relevant laboratory tests should be ordered for early diagnosis and treatment.

Objective To observe the effect of triplex surgical treatment, including rectal mucosa ligation,Xiaozhiling injection,encircing fighting surgery of the anus ring,on complete rectum prolapse. Methods From February 2007 to February 2010,fifteen cases with complete rectum prolapse were enrolled into this study and accepted triplex surgical treatment All patients were followed up for 1-4 years. Results The curative rate of the triplex surgical treatment was 100% with no serious complications. Conclusion Triplex surgical treatment was a simple, effective and reliable method in treating complete rectum prolapse, with less pain,fewer complications and quicker recovery.

Despite detailed examinations have been performed,the etiology in about one third of ischemic strokes remain uncertain,which are called cryptogenic ischemic strokes.In the recent 2 decades,it has been found that patent foramen ovale(PFO)is closely associated with these cryptogenic strokes,and a roamber of profound studies have been performed subsequently,and a lot of new conceptions have been put forward.INs article reviews advances in research on the relationship between PFO and iscbemic stroke.influencing factors of PFO leading to ischemic stroke,as well as the diagnosis and treatment of PFO.

Objective To study the differences of CXCR7 expression between in osteosarcoma tissues and normal peri-osteosarcoma tissues, and observe the effect on cell proliferation and inflammatory cytokine PGE2 secretion in MG63 cells when it had been transfected with pEGFP-Adv-CXCR7 vector. Methods CXCR7 expression in osteosarcoma tissues, cells and normal peri-osteosarcoma tissues detected by RT-qPCR. The CXCR7 mRNA expression, cell proliferation and PGE2 secretion of MG63 cells after transfected with pEGFP-Adv-CXCR7 expression vector were evaluated by RT-qPCR, MTT and ELISA, respectively. Results The expression rate and quality of CXCR7 mRNA in osteosarcoma tissue were 93.80% and (2. 221 ±0. 112), which significantly higher than those of normal peri-osteosarcoma tissues 45.71% and (0. 3448 ±0.098). Low expression of CXCR7 in MG63 cells was also observed. The proliferation and PGE2 secretion of MG63 cells were significantly increased at the time point of 48, 72 and 96h after pEGFPAdv-CXCR7 expression vector transfected into MG63 cells ( P < 0. 05). Conclusion The expression of CXCR7 mRNA in osteosarcoma tissues were significantly higher than normal peri-osteosarcoma tissues. CXCR7 high expression may have a negative effect on the progress of osteosarcoma.