Based on X86 architecture,a specialized software system was developed for liver teaching,imaging diagnosis and treatment.With MAYA as 3D modeling tool and DirectX3D as major virtual technology,a virtual liver model was created assisted by artificial intelligence and J2EE network communication.In addition,a virtual reality workstation about liver was established based on advanced modelling technology,human-computer interaction and database system to achieve a three-dimensional visualization of the liver on the virtual reality workstation.This system offers a feasible project for real-time chiri-cal consmlfation and teaching,and provides an extensive application of liver study under virtual condition.

Objective To explore the correlation of pregnant metaphase serum CRP and premature rupture of membranes and preterm birth.Methods Totally 300 cases maternals in pregnant metaphase were sampled to collected their fasting venous blood in the middle of elbow to detected the levels of serum CRP by immunoturbidimetry,and allof the maternals were received follow-up until end of the delivery.Of the 300 cases,there were 16 cases with premature rupture of membranes,28 cases with preterm birth,and 256 cases with full -term birth.The levels of serum CRP in pregnant metaphase of the three groups were compared,and the value of threshold to predict premature rupture of membranes and preterm birth was analyzed.Results The levels of serum CRP in pregnant metaphase of the groups of premature rupture of membranes and preterm birth were hoth higher than the group of full-term birth(P ＜ 0.05),but there were not significant difference between the two groups(P ＞ 0.05),and the threshold of the serum CRP were 13.9,10.8mg/L respectively.The sensitivity of serum CRP in pregnant metaphase to predict premature rupture of membranes was 68.75％,and the specificity was 98.94％.The sensitivity of serum CRP in pregnant metaphaseto predict preterm birth was 75.00％,and the specificity was 98.90％.Conclusion The level of pregnant metaphase serum CRP beyond a certain range may increase the possibility of premature rupture of membranes and preterm birth,which is valuable to predict premature rupture of membranes and preterm birth.

Objective To investigate the effect of Xuezhokang treatment on blood pressure and pulse pressure(PP) of elderly patients with primary hypertension(PH). Methods Fifty-eight elderly PH patients were treated with Amlodipine(5 mg/d).After the blood pressure were decreased to mild hypertension level,all the patients were randomly divided into 2 groups:30 patients in the Xuezhikang group,and 28 patients in the control group.The patients in the control group were treated with low fat and low salt diet and Amlodipine as before,and the patients in Xuezhikang group were treated not only with Xuezhikang 300 mg twice a day and Amlodipine as before for 12 weeks.Results The SBP and PP(mm Hg)were improved in both Xuezhikang and control groups,and Xuezhikang showed a better therapeutic effect(P＜0.05)than did the control.Xuezhikang group had SBP of(149±9,pretreatment)and(130±8,posttreatment),and PP of(64±11,pretreatment)and (48±7,posttreatment)(all P＜0.05).Control group showed SBP of(144±9)and(130±8)in pre-and post-treatment respectively,and showed PP of(60±10,pre-Rx)and(48±7,post-Rx)(all P＜0.05). Conclusions Anti-hypertensive medications in a routine dose combined with Xuezhikang have a better effect on the hypertension treatment in elderly patients.

Objective To evaluate the diagnostic value of magnetic resonance tomographic angiography(MRTA) with 3.0T scanner for neurovascular compression in patients with trigeminal neuralgia and hemifacial spasm.Methods MRTA using three-dimensional time-of-flight(TOF) sequence was performed in 52 cases with trigeminal neuralgia and 9 cases with hemifacial spasm.MR oblique sagittal and coronal images were created to display the relationship between trigeminal and facial nerves and surrounding vessels,and compared with that of operation MRA showed that.Results In 52 cases with trigeminal neuralgia,the trigeminal nerves were compressed by vessels or contacted with vessels in 46 cases at MRTA,and in 9 cases with hemifacial spasm,all trigeminal nerves showed having vascular compression or in contact with vessels.The accuracy,sensitivity and specificity of MRA in diagnosing trigeminal neuralgia and hemifacial spasm caused by vascular compression were 90.2%,96.4% and 80%,respectively,which compared with that of operation.Conclusion MRTA is sensitive to neurovascular compression,which plays an important role in diagnosing trigeminal neuralgia and hemifacial spasm.

Aged ; Amyloidosis ; complications ; diagnosis ; Diagnostic Errors ; Dyspnea ; etiology ; Edema ; etiology ; Female ; Humans ; Immunoglobulin Light-chain Amyloidosis

Objective Based on real-time PCR technique and ring primers,to establish a simple,accurate,cost-effective and easily standardized quantitative assay for quantification of HER2 mRNA,and apply to provide medication guidance for clinical tumor personalized molecular targeted therapy.Methods Screening reference gene which was stable expression in breast cancer,and optimizing the PCR reaction system.Then a real-time PCR with Eva Green for quantification of the mRNA expression levels of HER2 gene was developed.The specificity,sensitivity and reproducibility of the method were evaluated 87 specimens including 55 liquid nitrogen-frozen breast cancer tissues and 32 normal tissues were detected by the real-time quantitative reverse transcription (FQ RT)-PCR and immunohistochemistry(IHC).Results The standard curve of the method indicated a good linear relationship between the Ct value and the template concentration with the correlation coefficient being 0.997.The linear range of the system was from 101 to 106 copies/μl and the lower detection limit was 101 copies/μl.It had a high sensitivity and good specificity.The inter-assay coefficients of variation of HER and RPL37A genes were (5.93 ± 0.57)％ and (5.11 ± 0.59)％,(2.49 ±0.81)％ and (2.98 ±0.97)％ respectively.The intra-assay coefficients of variation were (5.76 ±0.58)％and (7.71 ±0.61)％,(3.75 ±0.76)％ and (4.40 ±0.96)％ respectively.Using the optimized FQ RTPCR system,HER2 gene of 87 specimens was quantificated.The sensitivity of the assay was 96.36％ (53/55),the specificity was 78.13％ (25/32),the positive predictive value was 88.33％ (53/60),the negative predictive value was 92.59％ (25/27),and the total coincidence rate between FQ RT-PCR and IHC was 89.66％ (78/87).The correlation of the results between the FQ RT-PCR and IHC was good (Kappa =0.770,P ＞ 0.05).Conclusions The method can quantify the mRNA expression levels of HER2 gene rapidly and cost-effictively with high sensitivity and specificity.It can be applied to clinical molecular diagnosis with attractive prospect.

Based on the point of view that the human body is an organic whole and the fact that virus and body is a unity of contradiction,the interaction mechanism of virus and immunity in the research of the anti-HIV/AIDS Chinese materia medica is discussed.On the one hand,it can widen our thinking to find new target of anti-virus drugs.On the other hand,it can also provide scientific support for understanding the complex mechanics of the anti-HIV/AIDS Chinese materia medica.

AIM: To investigate the details of age-related changes of T lymphocytes in order to seek for sensitive biomarker for immunosenesence. METHODS: Heparin anticoagulated venous blood was collected freshly from young (20-35 years) and elderly (50-75 years) volunteers and three or four color immunofluorescence staining was performed. The nucleated cells were acquired and the phenotypes of T lymphocyte subpopulations were analyzed with flow cytometry. RESULTS: There was no significant difference in percentages of pan-T (CD3 +), helper T (CD4 +) and cytotoxic (CD8 +) T subsets between young and elderly, whereas the density of CD3 molecule (MFI) on T cells in elderly group decreased significantly. It was also found that the rates of CD44 + and CD62L + T cell subsets in young group did not have statistical difference from elderly. However,the rates of CD95 + pan-T, helper T and cytotoxic T subsets of elderly group were all markedly higher than that in young group. CONCLUSIONS: The relative rates of T cell and its subsets displayed no age-related changes while the density of CD3 was down-regulated during aging in these groups investigated. Moreover, the expression percentage of CD95 (Fas) on T cells increased as aging, suggesting that it is a potential biomarker for evaluating immunosenescence.

AIM: To construct the mammalian cell expression vector for enhanced green fluorescent protein (EGFP) and HLA-A*0201 fusion protein and analyze its expression and subcellular localization in the transfected K562 cells. METHODS: The HLA-A*0201 cDNA was cloned by RT-PCR and the gene was inserted into pEGFP-N1 to construct a vector for the fusion protein. The expression of the fusion protein in K562 cells transfected with the vector was evaluated by flow cytometry and its subcellular localization was investigated by confocal microscopy. RESULTS: The full-length encoding region of HLA-A*0201 cDNA was cloned from two HLA-A2 positive donors and the expression vector for the HLA-A*0201-EGFP fusion protein was constructed by PCR using a primer pair to introduce a Kozak sequence before ATG and the stop codon was deleted. Five hours after K562 cells was transfected with the vector, the expression percentages of HLA-A*0201 and EGFP were 25.12?2.26 and 27.37?3.59, respectively and no significant increase was observed after 24 h. The fusion protein was predominantly located on the membrane with low level distribution within the cells. In contrast, no HLA-A*0201 but only EGFP was detected in the empty vector transfected K562 cells and the EGFP was dispersed within the cells. CONCLUSIONS: The expression vector for HLA-A*0201-EGFP fusion protein was constructed and the fusion protein expressed in K562 cells was primarily distributed on the membrane. The results suggest that the transfected K562 cells are potential antigen-presenting cells.

PurposeSpinal cord is one of the most frequently involved sites of multiple sclerosis (MS), which seriously affects the life quality of patients. In this paper, we investigate the application value of voxel-based morphology (VBM) and resting-state magnetic resonance imaging (RS-fMRI) in multiple sclerosis patients with single spinal cord involvement (MS-SSCI).Materials and Methods Three-dimensional T1WI data and RS-fMRI data were acquired from 20 patients with MS-SSCI and 20 normal controls, grey matter volume (GMV), changes of white matter volume (WMV), total intracranial volume (TIV) and local nuclei volume were compared between the two groups using VBM, posterior cingulate cortex (PCC) was regarded as the seed point and the functional connectivity about whole brain was compared between the two groups by using resting-state functional connectivity analysis, the relationships between MS-SSCI structure, function change parameters and expanded disability states scale (EDSS) scores were further explored.Results①Compared with the control group, GMV, WMV, TIV of MS-SSCI group were not significantly reduced, only the volume of some regions (bilateral middle temporal gyrus, left inferior temporal gyrus) showed significant atrophy (P<0.01); MS-SSCI exhibited increased functional connectivity (FC) in the left medial prefrontal cortex, left inferior temporal gyrus, left caudate nucleus and right supplementary motor area (two-sample t test, after AlphaSim correction,P<0.01, voxel size >40).②There was no significant correlation (P>0.05) between MS-SSCI structure change parameters and EDSS; while a significant correlation between EDSS scores and FC was noted in the left inferior temporal gyrus (r=0.633,P<0.05).Conclusion Both structural abnormalities and altered FC with PCC can be detected in MS-SSCI, but only functional parameters are associated with clinical abnormalities, which are more sensitive than microstructural changes.