1.Effect of basic fibroblast growth factor on the gene expression of decorin in periodontal ligament cells
Yuan CHENG ; Zhaofang ZENG ; Jun ZHU
Chinese Journal of Tissue Engineering Research 2010;14(11):2068-2071
OBJECTIVE:Studies have confirmed that basic fibroblast growth factor stimulating periodontal ligament cells can promote the proliferation of human periodontal ligament cells.so as to facilitate the reconstruction of lost periodontal tissues.By use of different concentrations of basic fibroblast growth factor,the in vitro cultured normal human periodontal ligament cells were stimulated to observe the decorin gene expression in the periodontal ligament cells.METHODS:The periodontal ligament cells were isolated and cultured using trypsin digestion method.The third generation of cells at logarithmic growth phase were preserved in DMEM containing 10% DMSO and 20% FBS frozen crvopreservation fluid,then moved into liquid nitrogen preservation at the second day Immunohistochemistry was used to identify anti-vimentin and cytokeratin staining The sixth generation of human periodontal ligament cells were divided into experimental group and control group.The experimental group was cultured in DMEM culture medium containing basic fibroblast growth factor at the concentration of 0.1,1,10 μg/L under standard conditions for 24 hours;control group was cultured with DMEM culture medium under standard conditions for 24 hours.The intracellular decorin gene expression was determined using RT-PCR.RESULTS:By microscopic observation,the cells in the control group had no significant changes,and cells proliferated in the experimental group,before stimulation the cells at the bottom of the bottle became denser than those in sparse regions.Adding basic fibroblast growth factor into periodontal ligament cells,could significantly decrease the mRNA expression of decorin,along with the increase of basic fibroblast growth factor concentration,the inhibition effect gradually weakened and became the strongest in the 0.1 μg/L but the weakest at 10 μg/L.CONCLUSION:The basic fibroblast growth factor stimulation can promote the proliferation of periodontal ligament cells and inhibit the synthesis of decorin in a dose-dependent manner,0 1 μg/L induces the strongest inhibitory effect.
2.Investigation on an incident of occupational contact dermatitis in a farm
Baofeng LIU ; Runan QIN ; Xudong LI ; Jun ZHU ; Qiang ZENG
Journal of Preventive Medicine 2022;34(3):294-296
Objective:
To investigate the cause of an incident of occupational contact dermatitis in a farm in Tianjin Prefecture, so as to provide insights into occupational safety.
Methods:
The disinfection process, use of disinfectants and individual protective measures in this farm were collected, and a field epidemiological investigation was conducted to collect the demographic characteristics, history of occupational contact, clinical symptoms, diagnosis and treatment data, and onset of disease among individuals with the same type of job. The cause of this incident was analyzed.
Results:
There were ten interns exposed to potassium hydrogen sulfate compounds simultaneously in this farm, and then, nine interns developed skin flushing across the body, and swelling and itching of the skin. Among these ten interns, five individuals were admitted to hospitals because of severe symptoms and were then clinically diagnosed as systemic contact dermatitis. All five individuals were cured following treatments. Epidemiological survey showed that all cases had a definite history of occupational contact with potassium hydrogen sulfate compounds but without use of any protective agents. In addition, there were thirty-five individuals with the same type of job in this farm that developed similar symptoms when they joined in the disinfection for the first time, and these individuals were self-cured following cessation to contact; however, recurrence of symptoms was found following contacts again.
Conclusion
This is a cluster of occupational contact dermatitis caused by exposure to potassium hydrogen sulfate compound.
3.No significant effect of iodinated contrast after single percutaneous coronary intervention on thyroid function and auto-antibodies
Xiaoyun ZENG ; You CHEN ; Jun ZHU ; Wei WEI ; Li ZHANG
Chinese Journal of Endocrinology and Metabolism 2013;(3):250-251
The effect of iodinated contrast on thyroid function and auto-antibodies after a single percutaneous coronary intervention (PCI) was explored.In 96 patients with randomly selected PCI,serum TSH,T3,T4,thyroid peroxidase antibody (TPOAb),thyroglobulin antibody (TgAb) were determined preoperatively as well as by 3-6 months postoperatively.After PCI,the level of TSH,T3,T4,showed no significant changes; TgAb,TPOAb which were positive preoperatively,after 3-6 months were statistically increased(P<0.05).Iodinated contrast in PCI has no significant influence on thyroid function and auto-antibodies after 3-6 months.
4.Effect of alendronate on proliferation of human osteoblast and the mRNA expression of osteoprotegerin and receptor activator of nuclear factor kappa B ligand
Yun ZHU ; Shirong CHEN ; Jun ZENG ; Lihua PENG
Chinese Journal of Tissue Engineering Research 2007;0(15):-
BACKGROUND:Alendronate is a new generation of diphosphate,which is the second generation of osteoporosis drugs.It is widely used to treat diseases related to increase of bone absorption in clinic.OBJECTIVE:To observe the effect of alendronate on the proliferation and the mRNA expression of osteoprotegerin(OPG) and receptor activator of nuclear factor kappa B ligand(RANKL) of human osteoblasts.DESIGN,TIME AND SETTING:A single sample observational experiment was performed at the Basic Research Institute of Chongqing Medical University from November 2007 to March 2008.MATERIALS:Osteoblasts were isolated from cancellous bone in a surgical operation.Alendronate was the product of Merck Sharp & Dohme S.p.A.METHODS:Osteoblasts were cultured with various concentrations of alendronate(1?10-9,1?10-8,1?10-7,1?10-6,1?10-5,1?10-4 mol/L).Osteoblasts cultured without alendronate were assigned as controls.MAIN OUTC0ME MEASURES:①The morphology and growth of osteoblasts were observed.②Effect of alendronate on the proliferation of osteoblasts were detected by MMT method.③The effect of various concentrations of alendronate on the mRNA expression of OPG and RANKL were detected by using RT-PCR.RESULTS:1?10-5 and 1?10-4 mol/L alendronate inhibited the proliferation of osteoblasts.1?10-9-1?10-6 mol/L alendronate promoted the proliferation of osteoblasts,and the 1?10-8 mol/L alendronate had the strongest effect.Alendronate inhibited RANKL mRNA expression in a concentration-dependent manner,1?10-5 mol/L alendronate had a strongest effect at 72 hours after culture(P
5.Outcome analysis of endemic fluorosis control in Guangxi in 2006
Min, LIAO ; Jun, LIU ; Zhen-zhu, TANG ; Li-rong, HUANG ; Rui-jun, NING ; Xuan-ping, ZENG
Chinese Journal of Endemiology 2008;27(3):300-302
Objective To investigate the distribution of water fluoride and the status of water-improving defluoridation projects,thus to explore the condition of endemic fluorosis in Guangxi Province.Methods According to"The National Technical Scheme for Endemic Disease Contml in 2005",the fuorine content in water Was determined by F-ion selective electrode,children's dental fluorosis was checked by Dean method.and the skeletal fluorosis was checked by the standard of clinical scale of skeletal fluorosis.Results 305 water samples in 61 villages were examined,among which 71 waters were exceeded the standard,accounting for 23.28%(71/305).The projects of defluoriding drinking water were running well except one was discarded.The prevalence of dental fluorosis was 13.55%(356/2627),the prevalence of skeletal fluorosis was 4.02%(65/1615).Conclusions The situation of endemic fluorosis control is not optimistic in Guangxi,which needs fuaher prevention and controls.
6.Correlation between di-2-ethylhexyl phthalate and idiopathic oligoasthenospermia.
Dian-Jun GAO ; Bo GAO ; Zeng-Jun ZHU ; Gang LIU ; Bo YANG
National Journal of Andrology 2014;20(8):702-705
OBJECTIVETo investigate the relationship between the level of di-2-ethylhexyl phthalate (DEHP) and idiopathic oligoasthenospermia by measuring the content of DEHP in the semen samples of different subjects.
METHODSWe obtained semen samples from 100 infertile men with idiopathic oligoasthenospermia, 50 working all the year round in the plastic greenhouse (group A) and the other 50 constantly dining from plastic meal boxes (group B). We also enrolled 50 normal male volunteers as controls (group C). We conducted semen analyses using a computer-assisted sperm analyzer, measured the DEHP concentration by reversed-phase high-pressure liquid chromatography, and subjected the data to statistic processing by t-test and correlation analysis.
RESULTSThe mean concentrations of DEHP in the seminal plasma were (0.72 +/- 0.48), (0.71 +/- 0.49) and (0.21 +/- 0.18) mg/L in groups A, B and C, respectively, significantly higher in A and B than in C (both P < 0.05). The DEHP concentration was negatively correlated with sperm motility (P < 0.05).
CONCLUSIONThe DEHP level in the seminal plasma is higher in infertile men frequently exposed to plastic products than in normal males and excessive DEHP may be one of the important factors of idiopathic male infertility.
Adult ; Case-Control Studies ; Diethylhexyl Phthalate ; adverse effects ; Humans ; Male ; Oligospermia ; etiology ; Plastics ; adverse effects ; Semen ; chemistry
7.Flow cytometric immunophenotyping of fine-needle aspiration specimens
Nenggang JIANG ; Jun SU ; Xueying SU ; Huanling ZHU ; Tingting ZENG ; Yongqian JIA
Chinese Journal of Laboratory Medicine 2009;32(6):649-653
Objective Comparative evaluation of flow cytometric immunophenotyping in the diagnosis and differentiation of lymphadenopathy,lymphoma and reactive lymphoid hyperplasia. Methods Ninty-nine fine-needle aspiration specimens from patients with tentative clinical lymphoprofierative disorders were consecutively analyzed by both cytology and flow cytometry with histology results as the gold standard. The three color antibodies including CD3,CD3,CD4,CD5,CD10,CD19,CD20,CD23,CD45,K,λ,FMC7 and CD34 were used for cell composition evaluation and cells with abnormal phenotype. Lymphoma cases were classified according to new WHO classification and subtypes were categorized by immunophenotypic analysis. The results from flow cytometry and cytology were compared. Results By cytological study, 40 of 99 cases were diagnosed with lymphoma, 29 cases were diagnosed with metastatic carcinoma, and 30 cases were diagnosed with reactive lymphoid hyperplasia, necrosis or tuberculosis. Among them, 2 non-Hodgkin lymphoma(NHL) cases were misdiagnosed as reactive lymphoid hyperplasia by cytology. Biopsy was performed in 18 cases of NHL including 16 B-NHL and 2 T-NHL By flow cytometry study, 35 of 99 eases were diagnosed with lymphoma, including 4 cases of lymphoblast lymphoma, 1 case of T-cell lymphoma, and 30 eases of other B-NHL For those 30 cases of B-NHL, 28 cases showed monoclonal light chain expression, and k: λ orλ: k atios exceed 3: 1, and B-cell proportion was (73. 2±27. 2)%. Twenty-six cases could be sub-classified by immunophenotyped. Among 16 histologically confirmed B-NHL cases, only 2 cases diagnosed with follicular lymphoma showed discrepancy with flow cytometry results. In all cases diagnosed with reactive lymphoid hyperplasia and metastasis carcinoma , no abnormal lymphocytes can be found, and k: λ or k: λ ratios were less than 3: 1. Conclusions Fine-needle aspiration analysis with flow eytometrie immunophenotyping can be helpful in diagnosis and differential diagnosis as well as sub-classification of NHL
8.Age-dependent Expression of GAP-43,Netrin-1,Collapsin-1,and Neuropilin-1 in Murine Cerebellum
Ning ZHU ; Jun MA ; Shaoju ZENG ; Yutao LIN ; Xinwen ZHANG ; Mingxue ZUO
Progress in Biochemistry and Biophysics 2009;36(6):750-760
GAP-43,netrin-1,collapsin-1,and neuropilin-1 have been regarded to play crucial roles in the formation of patterned neural connections.The cerebellum consists of five distinct concentric layers:white matter,internal granule layer (IGL),Purkinje cell layer (PCL),molecular layer (ML),and external granule layer (EGL) in young rodents.Cells in EGL are generated after birth.In contrast Purkinje neurons are born before birth,which receive main innervations of climbing fibers fi'om the inferior olivary nucleus and parallel fibers from the internal granule cells.These innervations are mostly established in the first three postnatal weeks,accompanying the sprouting and maturation of Purkinje cells.The potential roles of GAP-43,netrin-1,collapsin-1 and neuropilin-1 in the postnatal development of cerebellum remain unclear.To get insights into the above issue,the expression of GAP-43,netrin-1,collapsin-1,and neuropilin-1 mRNAs and proteins were examined in the cerebellum of mice at postnatal days (P) 5,P10,P20 and adulthood.The results showed that these four molecules were expressed in different temporal and spatial patterns in the postnatal cerebellum of mice,which was in match with axonal synaptogenesis,elongation and synapse formation during postnatal development and adulthood.By using double immunohistocbemistry,it was found that the Purkinje cells stained for GAP-43 were also positive for either netrin-1 or collapsin-1 at P10,and cells stained for collapsin-1 were also positive for netrin-1 or neuropilin-1.It was suggested that the four molecules are involved in the postnatal development of cerebellum.
9.Clinical evaluation study on long-term effect of acupuncture with pattern/syndrome differentiation on functional dyspepsia.
Duo-Duo LI ; Zeng-Hui YUE ; Li-Chao XU ; Tao XIE ; Gang-Zhu HU ; Jun YANG
Chinese Acupuncture & Moxibustion 2014;34(5):431-434
OBJECTIVETo compare the efficacy difference in the treatment of functional dyspepsia between acupuncture at the acupoints selected by pattern/syndrome differentiation and domperidone.
METHODSSeventy cases were randomized into an acupuncture group (35 cases) and a western medication group (35 cases). In the acupuncture group, Zusanli (ST 36) and Neiguan (PC 6) were selected. Taichong (LR 3) and Neiting (ST 44) were added for excess syndrome while Gongsun (SP 4) and Yinlingquan (SP 9) were added for deficiency syndrome. A pair of electrodes was attached to one acupoint and an assistant point (2 mm next to the acupoint centripetally) and stimulated with disperse-dense wave at 2 Hz/100 Hz, once a day. In the western medication group, domperidone was prescribed for oral administration, 10 mg each time, three times a day. In the two groups, the treatment of 5 days made one session and 4 sessions were required totally. Nepean dyspepsia index (NDI) was compared after treatment, 1, 2, 3, 4 and 5 months after treatment between the two groups respectively.
RESULTSThe score of symptom and score of life quality in NDI after treatment and at each follow-up time point were improved obviously in the acupuncture group as compared with those before treatment (all P < 0.01). In the western mediation group, the score of symptom and the score of life quality in NDI after treatment and in follow-up of 1, 2 and 3 months were improved obviously as compared with those before treatment (all P < 0.01), but the differences were not significant in follow-up of 4 and 5 months (both P > 0.05). Compared with the western medication group, the symptom score of NDI was reduced obviously after treatment and in each time point of follow-up in the acupuncture group (P < 0.05, P < 0.01), and the score of life quality was increased obviously (P < 0.05, P < 0.01).
CONCLUSIONAcupuncture at the acupoints selected by pattern/syndrome differentiation and domperidone are effective in the treatment of functional dyspepsia. Domperidone is unsatisfactory in the long-term effect, but acupuncture achieves the positive short-term and long-term effects on functional dyspepsia.
Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Dyspepsia ; diagnosis ; psychology ; therapy ; Female ; Humans ; Male ; Middle Aged ; Quality of Life ; Time ; Treatment Outcome ; Young Adult
10.Granuloma-like interdigitating dendritic cell sarcoma: report of a case.
Rong-jun MAO ; Xiong-zeng ZHU ; Qi-ming LI ; Hui-qiong FANG
Chinese Journal of Pathology 2012;41(2):134-136
Antigens, CD
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metabolism
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Antigens, Differentiation, Myelomonocytic
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metabolism
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Dendritic Cell Sarcoma, Follicular
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metabolism
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pathology
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Dendritic Cell Sarcoma, Interdigitating
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Granuloma
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metabolism
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pathology
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surgery
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Head and Neck Neoplasms
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metabolism
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pathology
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surgery
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Humans
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Leukocyte Common Antigens
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metabolism
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Lymph Nodes
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metabolism
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pathology
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Lymphoma, Large-Cell, Anaplastic
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metabolism
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pathology
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Lymphoma, T-Cell
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metabolism
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pathology
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Male
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Middle Aged
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Neoplasms, Multiple Primary
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metabolism
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pathology
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surgery
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Receptors, Cell Surface
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metabolism
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S100 Proteins
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metabolism
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Tonsillar Neoplasms
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metabolism
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pathology
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secondary