1.Implementation of the guided method in Clinical Laboratory Instrument Course
Zhaofang ZENG ; Hua XIANG ; Jun ZHENG
Chinese Journal of Medical Education Research 2011;10(3):279-280
Guided method was used in the Clinical Laboratory Instrument courses to guide students with inquiry-based learning problems,breaking the traditional"cramming"teaching,learning and giving full play the initiative and enthusiasm,stimulateing students'interest in learning to optimize the teaching process and improve the quality of teaching.
2.Clinical analysis of 21 cases of cervical adenosquamous carcinoma
Hongning CAI ; Xufeng WU ; Qunying XIANG ; Yongyan XIONG ; Jun ZENG
Chinese Journal of Obstetrics and Gynecology 2008;43(2):124-127
Objective To explore the clinical diagnostic and therapeutic characteristics,prognostic factors of pailents with primary clear cell carcinoma of the cervix.Methods The clinical,pathologic and follow-up data of patients with primary clear cell carcinoma of the cervix treated in our hospital from Jan 2003 to Dec 2006 were collected and analyzed retrospectively.The relative literature was reviewed.ResultsFive patients with primary clear cell carcinoma of the cervix were treated(1 case stage Ⅰ b1,2 of stage Ⅰ b2,1of stage Ⅱ a,1 of stage Ⅳa).The mean age was 40.2 years(32 to 50 years).The primary symptom was mostly irregularly vaginal bleeding(3/5)and clinical type was predominantly(4/5)endophytie growth.The positive rate of cervical cytologic examination was 2/4,the negative rate of cervical human papillomavirus(HPV)DNA examination was 4/4.Serum CA125 level was abnormal(62.5 to 592.1 kU/L)before operation and when relapse occurred,and returned to normal after operation.All of five patients underwent operation,pathologic examination showed that three patients with infihration in deep 1/2 myomctrium of cervix,and two patients with infiltration in cervix-corpus juncture.Four patients underwent radical abdominal hysterectomy with systematic pelvic lymphadenectomy.All of four patients underwent four courses of chemotherapy with fluorouracil(5-FU)and carboplatin,one patient(stage Ⅱ a)was added with intracavitary brachytherapy.None of the four patients had relapse or metastasis after a follow-up of 10 to 44 months.The patient with stage Ⅳ a underwent firstly hysterectomy and prerectum mass removal.Pelvic relapse occurred three months after operation and the patient then underwent the second operation,external beam radiotherapy and intracavitary brachytherapy and 8 courses of chemotherapy with paclitaxel(taxol) and carboplatin.There was no relapse or metastasis after a follow-up of 26 months.Conclusions Primary clear cell carcinoma of the cervix may be unrelated to HPV infection.It shows predominantly endophytic growth and tends toward deep infiltration in cervix and extending to uterine corpus.Operation combined with chemotherapy with carboplatin and 5-FU or taxol may lead to relatively perfect short-term therapeutical effect.Serum CA125 can help to monitor prognosis.Objective To study the clinical characteristics,treatment modalities.and prognosis of cervical adenosquamous carcinoma.Methods The data of 21 patients with adenosquamous cervical cancer who were admitted into Zhongnan Hospital,Wuhan University from Jan 2001 to Dec 2005 were analyzed retrospectively.Six patients received Surgical therapy only and 15 patients received combined therapy.ResultsSeven cases were with positive pelvic lymph node metastases and 3 cases were with ovarian metastases.The median survival time of the combined therapy group and surgical therapy group was 54 and 20 months,respectively.In stage Ⅰ and stage Ⅱ patients,the median survival time of the combined therapy group and surgical therapy group was 66 and 20 months,respectively.The difference was significant between the two groups(P<0.05).ConclusionsCombined therapy should be given to patients with adenosquamous carcinoma of the cervix.Surgical therapy and chemotherapy play an important role in the management and prognosis of adenoquamous carcinoma of cervix.Preserve of ovary for patients with adenosquamous carcinoma of the cervix should only be done when the ovary is confirmed free from any malignant involvement by pathology.
4.Clinical analysis of chronic barium poisoning.
Sheng-Jun MENG ; Qing-Long LU ; Zeng-Xiang MAS
Chinese Journal of Industrial Hygiene and Occupational Diseases 2009;27(6):370-371
Adolescent
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Adult
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Barium
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poisoning
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Chronic Disease
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Female
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Humans
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Male
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Middle Aged
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Occupational Diseases
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diagnosis
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therapy
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Retrospective Studies
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Young Adult
5.Exploration and practice of building "Clinical testing of equipment" course
Zhaofang ZENG ; Hua XIANG ; Guoming XIE ; Jun ZHENG
Chinese Journal of Medical Education Research 2006;0(10):-
Clinical testing of equipment is an interdisciplinary professional and technical course,of which we made continuous innovation and practice by optimizing the curriculum system ,upgrading teaching contents,reforming teaching methods,opening up the construction of teach-ing materials,building the teaching staff and improving teaching quality management etc.After five years of positive efforts the remarkable teaching results were achieved.
6.Quality course construction of ‘the clinical laboratory equipment'
Zhaofang ZENG ; Hua XIANG ; Guoming XIE ; Jun ZHENG ; Huaquan RAN
Chinese Journal of Medical Education Research 2006;0(07):-
By updating their concepts of education,carrying out the reform of teaching content,teaching methods and means,strengthening the construction of teaching materials,creating a high standard teaching team,the author aims to implement the construction of quality course of clinical testing equipment and comprehensively improve the teaching quality.
7.Establishment and evaluation of methods for determinating cystic fibrosis transmembrane conductance regulator quantitatively.
Feng QIU ; Jie ZENG ; Kun LI ; Ai-jun CHEN ; Wan-xiang XU ; Ya NI
Chinese Journal of Applied Physiology 2015;31(2):154-157
OBJECTIVETo establish and evaluate a BA-ELISA method for the quantitative detection of cystic fibrosis transmembrane conductance regulator (CFTR) protein.
METHODSWe deliberately selected three tables of CFTR and made the synthetic peptide be expressed in E. coli, then used the antigen to immunize rabbits to obtain the anti-CFTR polyclonal serum. After that, 96 well plates were coated with the purified antibody against CFTR. The antigen CFTR which was extracted from human sperm was detected by anti-CFTR antibody labeled with biotin, horseradish peroxidase conjugated avidin, and the substrate. The concentrations of two kinds of antibodies and the experiment parameters were optimized. Thereby, the double antibody sandwich BA-ELISA method for the quantitative detection of CFTR protein was established. Furthermore, the reproducibility, specificity and so on were evaluated by clinical specimens of sperm.
RESULTSThe optimal concentration of coated anti-CFTR IgG was 4 µg/ml, while the biotin labeled anti-CFTR IgG was 10 µg/ml; the optimal blocking buffer was 1% BSA-PBST, the optimal time of the reaction between antigen and antibody was 60 min, the optimal chromogenic time was 15 min, the intra-assay and inter-assay coefficient were 2.16%-9.23% and 2.29%-11.71% respectively; The lowest detectable limit was 0.15 ng/ml; the standard curve had a good linear correlation of R2 = 0.962.
CONCLUSIONThe BA-ELISA method for the quantitative detection of CTFR protein is successfully established, and it is demonstrated that the method has strong specificity, high sensitivity and good reproducibility. It provides the basis and evidence of the further application of the method.
Animals ; Antibodies ; Cystic Fibrosis Transmembrane Conductance Regulator ; analysis ; Enzyme-Linked Immunosorbent Assay ; methods ; Escherichia coli ; Humans ; Peptides ; Rabbits ; Reproducibility of Results ; Sensitivity and Specificity
8.CT Technique Supplied by DSA System
Weichu XIANG ; Jun LI ; Zhihui CHEN ; Li LUO ; Xiaohua ZENG ; Lianting MA
Chinese Medical Equipment Journal 2003;0(10):-
Objective To study the clinical value of CT function supplied by DSA system. Methods 278 patients were scanned by Innova CT ,which was concomitant with Innova 3100 DSA system, to check fresh blood .In the PTC operation, Innova CT was used to direct the site,degree,deepness of needle. Results Innova CT found intracranial hemorrhage in 4 cases . The fresh blood were checked by helical -CT,too. Innova CT comparing with B type ultrasonograph ,the orientation of needle were more convenient, exact, faster. Conclusion Innova CT could perform CT scan in interventional room to save time for emergence patients. It is a great improvement that gets CT images by DSA system. There is better consistency between Helix CT and Innova CT.
10.Magnetic resonance imaging in the assessment of the temporomandibular joint structure following mandibular advancement.
Chinese Journal of Stomatology 2007;42(11):665-668
OBJECTIVETo investigate the changes in the disc-condyle-fossa relationship following mandibular advancement.
METHODSTwenty-five patients (10 boys and 15 girls, 11.1 +/- 1.1 years) with class II division 1 malocclusions were treated with Activator. The average treatment time was 10.9 months. Parasagittal MRIs in closed mouth position were analyzed before and after Activator treatment by means of the pair-t test.
RESULTSCa-Tm value [(-0.1 +/- 1.5) mm and (-0.6 +/- 0.9) mm] of the left temporomandibular joint (TMJ) and Ca-Ca' value [(2.3 +/- 0.8) mm and (1.8 +/- 0.5) mm] of the right TMJ were changed significantly after Activator treatment (P < 0.05). This indicated that the condyle was located anteriorly in the fossa. No significant changes were found in the disc-fossa relationship and disc-condyle relationship (P > 0.05).
CONCLUSIONSActivator could promote the growth of the mandible and at the same time, it had no apparent negative influence on TMJ function.
Adolescent ; Child ; Female ; Humans ; Male ; Malocclusion, Angle Class II ; pathology ; therapy ; Mandibular Advancement ; Postoperative Period ; Temporomandibular Joint ; pathology