Objective To investigate the damaging effect of sulfur mustard on the mitochondria in rat lymphocytes in vitro.Methods Rat spleen lymphocytes were isolated by density gradient centrifugation and cultured with 100 ?mol/L sulfur mustard in vitro.DNA ladderring was used to detect the cell apoptosis.Cyt-c release was measured by Western blotting.The mitochondria function was detected by MTT method.Fluorescent probe labeled with Rhodamine 123 was used to study mitochondria potential.Results The mitochondria potential and cell viability decreased at 4 h after treated with 100 ?mol/L sulfur mustard.There was a liner correlation between the mitochondria potential and cell viability in lymphocytes.The content of Cyt-c increased significantly at 4 h as compared with control group in Western blotting.Conclusion Sulfur mustard could damage the rat lymphocyte mitochondria significantly,and mitochondria participates in cytotoxic effect induced by the sulfur mustard.

Objective To investigate the protective effects of GSH on the mitochondria in rat lymphocyte in vitro.Methods Rat spleen lymphocytes were isolated by density gradient centrifugation and cultured with sulfur mustard of 100,500,1000 ?mol/L in vitro.The mitochondria function was detected by MTT.The changes of GSH were detected by fluorescence spectrophotometry and DNA fragments were also detected.Results There was a positive line correlation between the changes of mitochondria function and the GSH content(P

3D ultrasound reconstruction in 63 cases of the solid space occupying lesions of liver was reported.Among them,there were 34 primary liver cancer,20 liver metastases and 9 cases hamangioma.By 30 imaging,the internal structure of the tumor could be visualized in 71%(45/63); the relation of the tumor to the surrounding vessels was apparent in 65%(41/63); the margin of the tumor could be outlined in 56%(35/63).The vesucts indicate that 3D ultrasound is superior to conventional 2D sonography to show more accurately the space localization,internal structure and morphology of the lesion thus provides meaningful imaging diagnostic evidences clinicaly.

Objective To explore surgical technique and clinical outcome of transabdominal preperitoneal hernia repair(TAPP)and totally extraperitoneal hernia repair(TEP)in inguinal hernia repair.MethodsThe clinical data of 23 patients underwent laparoscopic herniorrhaphy were retrospectively analyzed.Nine patients were underwent TAPP,and 14 patients underwent TEP.ResultsNone of patients was changed to open operation.Only one patient of TEP was changed to TAPP.Average operative time was(82.1?40.6)min.Blood loss was(5.7?3.0)ml.Disruption of peritoneum happened to 2 patients underwent TEP.The rate of postoperative complications was 21.7%(5/23),which included serum swelling(1 case),scrotal emphysema(2 cases),transient neurapraxia in the area of repair(1 case)and urinary retention(1 case).No chronic pain,foreign body sensation and infection were found.Average hospitalization after operation was(4.3?0.9)d.No recurrence was observed during a follow-up period of 1-15 months.ConclusionLaparoscope herniorrhaphy is safe due to lower recurrence and complications.It also has the advantages of slight pain and rapid recovery.

Objective To investigate the causes of intra-abdominal hernia and improve the level of early (diagnosis) and therapy of intra-abdominal hernia. Methods A retrospective analysis of the clinical data of 68 cases of intra-abdominal hernia was made. Results The postoperative confirmed diagnosis was para-occal hernia in 1 patient;herniation of efferent jejunal loop through the space between the afferent jejunal loop and the mesocolon after Billroth II gastrectomy in 6 patients,one of which had bowel necrosis,all of the 6 patients were cured after reduction of the hernia or enterectomy after and enteroanastomsis;internal henia though (ruptured) pelvic (peritoneum) after Miles operation in 4 patients;internal hernia though the space between the (descending) colon and the lateral abdominal wall after colostomy of descending colon in 6 patients;internal (hernia) caused by (adhesion) of omentum to the intestinal wall of sigmoidostomy in 1 patient;internal hernia through hiatuses caused by postoperative or post-peritonitis adhesions to the peritoneum,ovaries,urinary (bladder),uterus,(intestinal) wall or between loops of intestine in 50 patients(73.5%),including 3 cases of bowel (necrosis).All of those patients were cured after reduction of the hernias,repair of the hiatuses,release of (adhesions) or enterectomy and enteroanastomosis. Conclusions Preoperative diagnosis of internal abdominal hernia is difficult.The possibility of internal abdominal hernia should be cansidered in patients with a history of operation,and who complain of frequent abdominal pain or bowel obstruction.Early diagnosis and prompt (operation) is necessary to prevent the occurrence of bowel strangulation and bowel necrosis.

Objective To investigate the association between the genetic variant in the promoter region of miR-34b/c and cancer risk.Methods Databases such as PubMed、EMBase、Wanfang、VIP、CNKI and so on were searched comprehensively.Based on the including and excluding criteria,literatures that were eligible were screened and data were retrieved.Meta analysis was performed by RevMan 5.1 software.Results Through searching and manually searching relevant references,a total of 9 articles with 10 independent studies were included.No significant associations were detected in C vs T,CC vs TT,CC + CT vs TT and CC vs CT + TT comparison models.However,in the CT vs TT comparison model,the result showed a significant association (Z =2.33,P =0.02).Meanwhile,subgroup analysis of hepatocellular carcinoma and colorectal cancer both showed significant associations with the polymorphism,with C vs T (OR =1.11,Z =2.10,P =0.04),CT vs TT (Z=2.40,P =0.02),CC +CT vs TT (Z=2.45,P=0.01) and CC vs TT (OR =0.66,Z=2.43,P=0.02),CC vs CT + TT (OR =0.67,Z =2.40,P =0.02) respectively.The C allele increased significantly the susceptibility of hepatocellular carcinoma,and the CC genotype reduced significantly the susceptibility of colorectal cancer.Conclusion The genetic variant in the promoter region of miR-34b/c rs4938723 T ＞ C is significantly associated with the susceptibility of hepatocellular carcinoma and colorectal cancer.

Objective To investigate the association between a genetic variant in Dicer and cancer risk .Methods Both English and Chi-nese databases were carefully searched comprehensively .Based on the including and excluding criteria ,literatures that were eligible were carefully screened and data were retrieved .Meta analysis was performed by RevMan 5 .1 software .Pooled odds ratios(OR) and 95% confi-dential interval(CI)were used for analysis .Results Ten eligible literatures were included in the meta analysis .Meta analysis showed that the mutant allele C in 3′untranslated region polymorphism loci rs1057035 of Dicer gene might reduce the cancer risk(OR=0 .92 ,95% CI=0 .87-0 .97 ,P=0 .003) .Conclusion The variant rs1057035 T>C in Dicer gene could be related to cancer susceptibility .

Acupuncture Therapy ; Adult ; Aged ; Colitis ; therapy ; Female ; Humans ; Male ; Middle Aged ; Young Adult

Objective:To detect the levels of TGF-β1 in hypertensive renal function impairment patients and its clinical value. Methods: Primary hypertension patients with no target organs damage were enrolled in control group, primary hypertension patients with renal function stageⅠand stageⅡwere enrolled in renal function stageⅠgroup and stageⅡgroup. Serum TGF-β1 levels and renal function indices of three groups were compared. Results:There were no significant difference of serum creatinine and blood urea nitrogen of three groups(F=23.523, P<0.05);endogenous creatinine clearance rate of renal functionⅠandⅡgroups were lower than that of control group, TGF-β1 levels of renal functionⅠandⅡ groups were higher than of control group; TGF-β1 levels were negatively correlated with creatinine clearance rate. Conclusion:TGF-β1 level is an ideal index to reflect renal function for its level significantly changes in renal damage stageⅠ,Ⅱand has good correlation with Cin.

Objective: To establish an enzyme linked immunosorbent assay(ELISA)for erythropoietin(EPO) in serum, and observe its clinical application value thereof. Methods: Prepare the EPO polyclonal antibody, wash the plate with isopropyl alcohol, and then choose the suitable concentration of the antibody, enzyme labeled antibody, and antigen. After the reaction, check the sensitivity, recovery, specificity and stability of the method. The serum samples of anaemia and breast carcinoma individuals were detected. The results of radioimmunodetection were compared with that of normal control group. Results: The immo-assay plate showed strong adherence to proteins. The optimal concentrations of the antibody, enzyme labelled antibody and antigen were 1∶1 000, 1∶6 000 and 1∶800 separately. The sensitivity was 0.46 U/L. The cross-reaction with growth hormone and ferritin was low. The mean recoveries of samples with high and low concentrations were 96.3%, 97.3% respectively. The coefficients of variation of intra-assay and inter-assay were just 8.31% and 7.82%, and the stability was good. The EPO levels were higher in anaemia and breast carcinoma groups than that of normal group. There was no significant difference between the results of the radioimmunodetection and ELISA. Conclusion: The double-antibody sandwich ELISA method was established for EPO in serum, which had certain clinical application value.