The complex field cure instrument is a new medical instrument with which an experiment was carried out. Rats were continuously irradiated by the complex field for 90 days, with a day's total dose of 285.9 M.T.G. while other rats weren't irradiated for control group. The animals were respectively killed at 7d, 14d, 30d, 60d and 90d, and their blood samples were taken for cell and humoral immune analysis. The results show that values of lymphocyte transform rate, soluble receptor (SIL-2R), total hemolytic complement levels (CH50) and immunoglobulin (A.G.) after irradiation are more than those of the control group having proved that the instrument may improve immune function of rats.
Animals ; Female ; Immunoglobulin A ; blood ; Immunoglobulin G ; blood ; Immunoglobulins ; blood ; Lymphocyte Activation ; Lymphocyte Count ; Male ; Physical Therapy Modalities ; instrumentation ; Rats ; Rats, Sprague-Dawley ; Receptors, Interleukin-2 ; blood ; Time Factors

To clear the effect of the wound to the growth of the larva of the host to the Ophiocordyceps sinensis, the wounds of same severity at the same position were made artificially to the larva and which were artificial fed at the same environment and condition. The results indicated that, over the winter, the survival rate of the wounded of the infection larva was lower than that of the healthy larva, but the weight had no significant difference between the wounded and the healthy larva. The survival rate of the wounded of the no infection larva was lower than that of the healthy larva, but except with black skin, the wounded larva with offwhite and dusty red had no influence on the variety of the weight. In summery, wound had no advantage to the survival rate, but had no influence to the weight. The result had provided theoretical basis to the reforming of the system of the artificial culture O. sinensis.
Animals ; Body Weight ; Breeding ; methods ; Hypocreales ; growth & development ; Larva ; Moths ; growth & development ; microbiology

OBJECTIVETo prepare a stable water-based magnetic fluid.

METHODSA water-based magnetic fluid was prepared by addition of polyvinylpyrrolidone (PVP) as the coating agent for the magnetic particles. After preparation of Fe3O4 by co-precipitation method, PVP was added for its coating, followed by ultrasonic agitation and purification.

RESULTSThe magnetic nanoparticles of homogeneously small size and water-based magnetic fluid were obtained, which had good dispersion in water with strong magnetism.

CONCLUSIONPVP can be used as a surfactant to stabilize the magnetic fluid.

Ferrous Compounds ; chemistry ; Magnetics ; Materials Testing ; Nanoparticles ; chemistry ; Povidone ; chemistry ; Surface Properties ; Surface-Active Agents ; chemical synthesis ; chemistry

UNLABELLEDTo study the effects of the integrated pest control techniques on growth of host larvae of Cordyceps sinensis.

METHODThe integrated pest control techniques were compared with conventional techniques to evaluate the effects on growth of host larvae.

RESULT AND CONCLUSIONThe results showed that the techniques had broken the balance of the microbial living in the material, produced effective inhibition on the pests, raised the survival rate and promoted the growth of the host larvae at the same time.

Animals ; Cordyceps ; physiology ; Larva ; growth & development ; microbiology ; Moths ; growth & development ; microbiology ; Pest Control, Biological ; methods

OBJECTIVETo study the effects of ulinastatin on coagulation in children who underwent open-heart surgery with cardiopulmonary bypass (CPB).

METHODSFifty children who underwent open-heart surgery for ventricular septal defect were randomly divided into two groups: ulinastatin treatment and control. Before CPB, ulinastatin (1.0×10(4) U/kg) was added to CPB priming fluid only in the ulinastatin treatment group. Activated partial thromboplasin time (APTT), prothrombin time (PT), thrombin time (TT), fibrinogen and international normalized ratio (INR) were measured both before and at 1 hr, 6 hrs and 24 hrs after CPB.

RESULTSThe PT in the ulinastatin group was more prolonged than in the control group at 1 hr after CPB (18.7 ± 0.7 s vs 15.5 ± 0.5 s) and 6 hrs after CPB (17.5 ± 0.6 s vs 15.0 ± 0.6 s). The APTT in the ulinatatin group was also significantly more prolonged than in the control group at 6 hrs after CPB (38.7 ± 3.1 s vs 35.3 ± 3.1 s) and 24 hrs after CPB (34.2 ± 3.0 s vs 31.1 ± 2.6 s).

CONCLUSIONSUlinastatin may prolong PT and APTT after CPB, and thus affects coagulation in children.

Blood Coagulation ; drug effects ; Cardiac Surgical Procedures ; Cardiopulmonary Bypass ; Female ; Glycoproteins ; pharmacology ; Humans ; Infant ; Male ; Partial Thromboplastin Time ; Prothrombin Time ; Trypsin Inhibitors ; pharmacology

Adult ; Aged ; Aged, 80 and over ; Female ; Humans ; Leukemia, Prolymphocytic, B-Cell ; diagnosis ; Male ; Middle Aged ; Retrospective Studies

Objective To observe the expression of silent information regulator 1(SIRT1)in esophageal squamous cell carcinoma,and its relationship with invasion and metastasis of esophageal squamous cell carcinoma and its effect on survival and prognosis.Methods The clinical and pathological data of 104 patients with esophageal cancer who underwent radical esophagectomy at the Affiliated Tumor Hospital of Xinjiang Medical University from January to December 2009 were retrospectively reviewed. Immunohistochemical method (EnVision method) was used to detect the expression of SIRT1 in cancer tissues and adjacent tissues. The clinicopathological features of different SIRT1 expressions were compared. The factors affecting the prognosis of the patients with esophageal cancer were analyzed.Results The positive expression rate of SIRT1 protein was significantly higher in esophageal squamous cell carcinoma tissues than that in adjacent tissues(61.54% vs. 29.81%, χ2=21.100, P<0.05). The expression of SIRT1 in esophageal squamous cell carcinoma was significantly correlated with vascular infiltration(VI),infiltration depth(pT),lymph node metastasis(pN)and clinical stage(pTNM)(P<0.05).Univariate survival analysis showed that the overall survival time(OS)was significantly lower in patients with SIRT1 positive expression than that of patients with negative expression(Log-rank χ2=10.065,P<0.05).SIRT1 positive expression, lymph node metastasis,deep tumor infiltration,late clinical stage and vascular infiltration showed poor prognosis(P<0.05). Cox multivariate regression analysis showed that SIRT1 positive expression, lymph node metastasis, depth of invasion and clinical stage were independent prognostic factors for esophageal squamous cell carcinoma. The prognosis of patients with deep tumor invasion, lymph node metastasis, late clinical stage and high expression of SIRT1 was worse. Conclusion SIRT1 is highly expressed in esophageal squamous cell carcinoma, and which is closely related to the invasion and metastasis of esophageal squamous cell carcinoma and has an effect on the prognosis of patients.

Objective To investigate the change of the basic fibroblast growth factor(bFGF) leve in human detrusor muscle(DM)in bladder outlet obstruction(BOO)due to benign prostatic hyperplasia(BPH)and its implication.Methods Fifty-four patients with BPH were divided into two groups:the obstructive DM stability and instability groups;and 15 men with bladder tumor who underwent operation in the same period were enrolled in the control group.The bFGF mRNA level in DM was measured by reverse transcription and polymerase chain reaction(RT-PCR)and the bFGF protein level was measured by immunohistochemical staining method.Results The bFGF-mRNA expression level of bladder smooth muscle cells was significantly lower in the control group than that in the obstructive DM stability and instability groups(all P＜0.05),but there was no significant difference between the obstructive DM stability and instability groups(P＞0.05). Conclusions The expression level of bFGF mRNA in bladder DM is elevated in BOO due to BPH,but there is little or no correlation between the increased expression of bFGF mRNA and detrusor muscle instability.

Objective To investigate the clinical method of closing defect and regain the sensory on forefoot injury.Methods Lateral tarsal artery,flap was designed as a reverse flow flap to close forefoot de- fect in dorsal lateral foot while perforating branche of lateral tarsal artery as turning point.Lateral cutaneous nerve was inosculated to lateral plantar fascia.Donor site was covered by skin-grafting.Results seventeen cases survived satisfactorily with good shape and regaining sensory.Conclusion Lateral tarsal artery flap can be used in coveraged of forefoot defect.Lateral tarsal artery flap was thin flap with good shape and to regain the sensory of forefoot.

Objective To determine the relationship between estimated glomerular filtration rate (eGFR) and proteinuria with cardiovascular events in subjects aged 80 years or older.Methods Data for this retrospective prognostic study were drawn from the patient database for routine checkup in Beijing hospital between January 2001 to December 2001.Baseline eGFR and proteinuria were evaluated in 340 subjects [mean age:(85.6 ± 4.0) years].eGFR was calculated using the modified abbreviated MDRD equations based on the Chinese chronic kidney disease patients.The subjects were divided into normal renal function group and reduced renal function group (eGFR ＜ 60 ml · min-1 · 1.73 m-2).The subjects were divided into subjects without proteinuria and subjects with proteinuria group.Cardiovascular events included cardiovascular death,nonfatal myocardial infarction,nonfatal stroke.Results The proportion of reduced renal function was 36.8％ (125/340).The proportion of proteinuria was 10.3％ (35/340).The proportion of reduced renal function or proteinuria was 41.8％ (142/340).Follow-up time was 79 months (40-114 months).Cardiovascular events rate was significantly higher in reduced renal function group than in normal renal function group [37.6％ (47/125)vs.26.2％ (55/210),P ＜0.05] and in proteinuria group than in without proteinuria group [50.0％ (17/34) vs.28.2％ (85/301),P ＜ 0.01].Cox multivariate analysis revealed that both eGFR (HR =0.978,95％ CI:0.961-0.994,P ＜ 0.05) and proteinuria (HR =2.049,95％ CI:1.132-3.709,P ＜ O.05) were independent risk factors for cardiovascular events after adjusting for age,gender,triglyceride,high-density lipoprotein cholesterol,low-density lipoprotein cholesterol,uric acid,hypertension,coronary heart disease,diabetes mellitus.Conclusions Reduced eGFR and presence of proteinuria are independent risk factors for cardiovascular event in subjects aged 80 years or older.eGFR and proteinuria can thus be used for cardiovascular event risk stratification in subjects aged 80 years or older.