Objective:To explore the clinical characteristics of senile degenerative valvular heart disease (SDHVD)compli-cated heart failure (HF).Methods:A total of 68 SDHVD + HF patients were enrolled as SDHVD + HF group,another 62 aged HF patients without valvular calcification in the same period were enrolled as HF control group.Characteristics of two groups were analyzed.Results:Compared with HF control group,there were significant rise in percentages of severe cardiac dysfunction (NYHA class III:19.35% vs.38.24%,NYHA class IV:35.49% vs.55.88%),incidence rates of at-rial premature (17.74% vs.38.24%),sinoatrial block (22.58% vs.50.00%),atrial fibrillation (27.42% vs.52.94%), paroxysmal atrial tachycardia (30.65% vs.48.53%),atrioventricular block (33.87% vs.52.94%)and bundle branch block (25.81% vs.48.53%)in SDHVD + HF group,P <0.05 or <0.01. Conclusion:When SDHVD patients compli-cate with HF,the HF degree aggravates and incidence rate of arrhythmia rises.

Objective To compare the outcomes of 23G and 25G plus (25G+) vitrectomy in treatment of proliferative diabetic retinopathy (PDR).Methods This is a prospective randomized study.Fifty-seven PDR patients (75 eyes) with symptoms requiring vitrectomy were randomly divided into 23G vitrectomy group (30 patients,39 eyes) and 25G+ vitrectomy group (27 patients,36 eyes).Visual acuity,intraocular pressures,ophthalmoscopy,B-scan ultrasound was examined before surgery.The follow-up period was 10.0 (23G group) and 8.5 months (25G+ group) respectively.Intraoperative complications,operation time,postoperative visual acuity,intraocular pressure,postoperative complications and postoperative ocular conditions were analyzed.Results The mean surgical times were (53.35± 7.42)minutes and (49.16±5.17) minutes in 23G and 25G+ group respectively,and the difference was significant (t=4.37,P＜0.05).Iatrogenic injuries occurred in 11 eyes (28.21％) and 5 (13.89％) eyes in 23G and 25G + group respectively,and the difference was significant (x2 =4.93,P＜0.05).The postoperative visual acuity of 23G and 25G+ group were improved compared to before surgery (x2=16.81,18.29; P＜0.05).At last follow-up,there was 25 eyes and 24 eyes with visual acuity ≥ 0.05 in 23G and 25G+ groups respectively,and the difference was not significant (x2 =0.13,P＞0.05).Hypotony was detected in 7 and 3 eyes at the third postoperative day in 23G and 25G+ group respectively,and the difference was significant (x2 =5.67,P＜0.05).Conclusion 25G+ vitrectomy is a safe and effective treatment for PDR with shorter surgery time and fewer surgical complications.

Objective To explore the clinical features and urgent treatment strategies of open stabbing and penetrating wound of the heart. Methods In recent five years, 22 cases of cardiac trauma patients were sent to our emergency department, among them 19 sustained penetrating wounds produced by sharp instruments, 3 with perforating wounds, produced by firearm in 2 and sharp instrument in one. Emergent thoracotomy was performed in all the patients. The injury involved right auricle in 4, left auricle in 1, right ventricle in 11, left ventricle in 3, and perforating wounds of both right and left ventricles in 3 patients. Results Among them 19 survived, and their cardiac and pulmonary recovered 2 weeks after the operation. Three patients with injury of the great vessel died, one of them died 8 hour after the operation. Conclusion When the patients who presented open wounds on the anterior or posterior chests, showing a low or undetectable blood pressure, which could not raised even with fluid resuscitation with two venous lines or in some of them displaying signs of Beck's triad, open cardiac injury should be seriously suspected, and emergent thoracotomy should be taken in order to rescue the patients.

Objective To explore the effects of DU meridian electroacupuncture on the survival,differentiation and immigration of neural stem cells(NSCs) transplanted in the injured site of rat spinal cord transection. Methods Twenty adult SD rats were divided into NSCs transplanted 14d group(NSCsl4d group),DU meridian electroacupuncture plus NSCs transplanted 14d group(EA+NSCs14d group),NSCs transplanted 30d group(NSCs30d group) and DU meridian electroacupuncture plus NSCs transplanted 30d group(EA+NSCs30d group).T10 spinal cord segments of all animals were completely transected.The rats of EA+NSCs14d group and EA+NSCs30d group were treated with DU electroacupuncture at 5 days after operation.The sacrifice times of the rats were respectively at 14 days and 30 days after operation.The spinal cords were taken for observing the survival,differentiation and immigration of neural stem cells transplanted in the injured site of rat spinal cord transection. Results 1.The surviving number of transplanted NSCs in EA+NSCs14d group or EA+NSCs30d group was more than that of NSCsl4d group or NSCs30d group,and the surviving number of transplanted NSCs in EA+NSCs30d group and NSCs30d group was less than that in EA+NSCs14d group and NSCs14d group.2.Some transplanted NSCs at injured site of transected completely spinal cord and neighboring tissue showed microtubule association protein 2(MAP2) positive staining in EA+NSCs30d group or NSCs30d group.3.Many transplanted NSCs at injured site of transected completely spinal cord and neighboring tissue could be observed to show glial fibrillary acidic protein(GFAP) positive staining in EA+NSCs 30d group or NSCs 30d group.4.The immigrating distance of transplanted NSCs toward caudal tissue of injured site of spinal cord was longer in EA+NSCs 14d group and EA+NSCs 30d group than that in NSCs 14d group and NSCs 30d group.Conclusion DU meridian electroacupuncture may promote the survival of neural stem cells transplanted in injured site of rat spinal cord transected completely,and these cells can differentiate into MAP2 or GFAP possive cells.DU meridian electoacupuncture may affect the immigrating direction of neural stem cells transplanted at injured site of spinal cord toward host spinal cord tissue.

Objective To summarize the incidence, diagnosis, treatment and prognosis of patients with hemolysis, elevated liver enzymes and low platelet count (HELLP) syndrome. Methods The clinical data of fourteen cases with HELLP syndrome complicated by severe pregnancy-induced hypertension were analyzed retrospectively during the past seven years. Results The incidence of HELLP syndrome was 8% in the patients with severe pregnancy-induced hypertension. According to the diagnosis criteria used by Tennessee University, there were eight cases with complete HELLP syndrome and six cases with partial HELLP syndrome. The major therapeutic way were intensive maternal and fetal monitoring, active management of preeclampsia and eclampsia, administration of corticosteroids and termination the pregnancy as fast as possible. The major complications were DIC, placental abruption, pneumonedema and acute renal failure. The maternal and perinatal mortality were 7% and 29%, respectively. Conclusions HELLP syndrome was a serious life-threatening complication of severe pregnancy- induced hypertension. In order to decrease the maternal and perinatal mortality rate, HELLP syndrome should be diagnosed and treated as fast as possible.

Objective To investigate serum cancer antigens (CA) 125 expression in different types of ovarian endometriosis and related factors.Methods A total of 182 patients with ovarian endometriosis was collected,then they were divided into two groups:ovarian endometriosis combined with other parts of lesions (A group,88 cases),simple ovarian endometriosis group (B group,94 cases).And selected 100 healthy physical check-up women from the hospital outpatient department were as control group.The serum level of CA125 was detected.The patients' information including body shape,menstrual factors,reproductive activities and disease staging were collected.Chi-square criterion and multivariate binary Logistic regression analysis were completed.Results The serum levels of CA125 in A group,B group and control group were (48.64 ± 33.18),(41.35 ± 23.46),(22.16 ± 13.73) kU/L,respectively,and the positive expression rate of three groups were 64.77％ (57/88),52.13％ (49/94),2.00％ (2/100),respectively,there were significant differences (P ＜ 0.05).The multivariate binary Logistic regression analysis result showed that age of 35-49years,reforming American fertility society of Ⅲ-Ⅳ stage,pelvic adhesion and menorrhalgia were the important factors of positive expression of CA125.Conclusion The serum level of CA125 can be used as the evaluation of the diagnosis and treatment of ovarian endometriosis.

This article reviews the major advances of basal and clinical research on the adrenal diseases at home and abroad from 2010 to 2012.There are many advances including the diagnostic value of steroidogenic factor-1 in adrenal tumors,the pathogenesis and new diagnostic methods of aldosterone-producing adenoma,the treatment for severe ACTH-dependent Cushing's syndrome,approach to the patient with an adrenal incidentaloma and subclinical hypercortisolism,screening test for subclinical hypercortisolism in the patients with diabetes or osteoporosis,the characterization of macronodular adrenocortical hyperplasia of the zona reticularis,as well as genetic testing for pheochromocytoma and paragangliomas,etc.

3D ultrasound reconstruction in 63 cases of the solid space occupying lesions of liver was reported.Among them,there were 34 primary liver cancer,20 liver metastases and 9 cases hamangioma.By 30 imaging,the internal structure of the tumor could be visualized in 71%(45/63); the relation of the tumor to the surrounding vessels was apparent in 65%(41/63); the margin of the tumor could be outlined in 56%(35/63).The vesucts indicate that 3D ultrasound is superior to conventional 2D sonography to show more accurately the space localization,internal structure and morphology of the lesion thus provides meaningful imaging diagnostic evidences clinicaly.

Zebrafish is widely used as a model organism in the process of drug discovery. It expresses drug metabolizing enzymes like cytochrome P450 (CYP450), uridine 5'-diphospho-glucuronosyltransferase (UGT) and nuclear receptors like pregnane X receptor (PXR), aryl hydrocarbon receptor (AHR), etc. This article summarized the profiles of main drug metabolizing enzymes and nuclear receptors, and reviewed the advances on xenobiotics metabolism in zebrafish.

Objective To explore the correlation between HBV-DNA and serum HBV markers quantitative detection and determine its diagnosis value in hepatitis B virus infection. Methods The HBV-DNA was measured by fluorescence quantitative PCR and hepatitis B virus was detected by time-resolved fluorescence immunoassay (TRFIA). The relationship between the two tests was analyzed statistically using software. Results Detection of HBV-DNA in the peripheral blood of patients with HBsAg (+), HBeAg (+), HBcAb ( + ) showed 92. 9% (79/85) positive rate,and the average DNA copy number was (4.31 ± 1.64) × 106 copies/ml. While the detection of HBV-DNA in the peripheral blood of patients with HBsAg ( + ), HBeAb ( + ), HBcAb ( + ) showed 47.7% (105/220) positive rate, and the average DNA copy number was (2.47 ± 2. 21 ) × 104 copies/ml. In normal controls,blood HBV-DNA detection was negative. Furthermore, HBV-DNA copy number correlated positively with HbeAg quantitation (r = 0. 59, P = 0. 041 ). However, we found no significant correlation between HBV-DNA copy number and HbsAg quantititation (r = 0.221, P = 0.077). Conclusions Different HBV-M had diverse correlation with HBV-DNA number, comprehensive detection of them would be better to assist clinical diagnosis and evaluate the response to treatments.