Objective To explore the risk factors of vascular dementia (VD) in stroke patients, expecial-ly, the relationship between VD and carotid artery atherosclerosis. Methods 64 patients with cerebrovascular dis-ease , who had a history of stroke , were divided into VD groups (n = 64) and control group (n = 60). The age, disease course, education and blood lipid and carotid artery stenosis were compared. Results The age of patients , course of disease ,educational levels, the serum levels of TC ,TG, LDL and internal diameter of carotid artery were different between the two groups (P < 0.05 ). Conclusion The age of patients , course of disease , education, the serum levels of TC ,TG ,LDL and carotid artery atherosclerosis are related to vascular dementia in stroke patients.

Objective To observe the effect of Shuxuening Injection NO, NOS, SOD and MDA on of angina pectoris patients. Methods 100 patients with angina pectoris were randomly divided into two groups. 50 patients in treatment group were treated with Shuxuening Injection, 5 mL once, drop into 250 mL 5% glucose injection (it change into 250 mL 0.9% sodium chloride injection if the patient have diabetes), one time a day. 50 patients in control group were treated with isosorbide mononitrate injection, 60 mg once, drop into 250 mL 5% glucose injection (it change into 250 mL 0.9% sodium chloride injection if the patient have diabetes), 15 gtt one minute, one time a day. Results NO, NOS and SOD of the treatment group were objective improved, and MDA objective reduced. NO improved from (47.61?8.76)?mol/L to (71.84? 13.26)?mol/L, NOS improved from (1.64?0.23)U/mL to (2.31?0.29)U/mL, SOD improved from (46.34? 5.62)U/mL to (64.54?9.10)U/mL, and MDA reduced from (8.96?1.76)U/mL to (6.71?1.43)U/mL. Total effective rate in improving angina pectoris was 96%, and ECG was 76%. The time (ischemic ST-T changes) of holter ECG was reduced from (130.45?18.61)min to (32.33?5.32)min, there was a significant difference between the two groups (P

Objective:To evaluate the effects of shade and light irradiation condition on the polymerization of composite resin core material.Methods:The light-proof silicon rubber cuboid mold with one end open were stringed and filled by dual-cured flowable composite resin core material with the shade of dentine and white(Paracore) respectively,then the open end of mold was irradiated directly by a light unit at 1 000 mW/cm2 ×20 s and 3 200 mW/cm2 ×6 s respectively.And all specimens of 4 groups(n =5) were stored in the light-proof box.1 h and 24 h after irradiation,Knoop microhardness was measured along the vertical surfaces of the specimens at the depth of 1,2 and 3 mm respectively.Data were analyzed by One-way ANOVA.Results:At all light irradiation conditions,the hardness of all specimens decreased with the increase of measurement depth.24 h after irradiation,the hardness of dentine shade specimens at each measurement depth showed no difference between 1 000 mW/cm2 × 20 s and 3 200 mW/cm2 × 6 s irradiation,while the white shade specimens irradiated by 1 000 mW/cm2 × 20 s showed higher hardness than those by 3 200 mW/cm2 × 6 s.Conclusion:To obtain sufficient polymerization of composite core material with different shade optimal light irradiation condition should be selected.

OBJECTIVE: To observe the clinical efficacy and safety of additional use of Cypate gingival fluid in periodontal basic therapy before surgery in the treatment of pregnancy gingival tumor. METHODS: A total of 86 patients with pregnancy gingival tumor in Second Affiliated Hospital of Qiqihar Medical University during Jan. 2015 to Jan. 2016 were divided into observation group and control group according to random number table, with 43 cases in each group. Control group received ligation and oral micro-electrosurgical surgery according to the volume of tumor. Observation group was given Cypate gingival fluid-based periodontal basic therapy (3-5 mL for gargling, 2-3 min each time, 3-5 times each day, 5 d) and then receive surgery. Clinical efficacies, gingival related indexes (gingival index, sulcular bleeding index, probing depth), quality scores of life, ADR, the occurrence of complications were observed in 2 groups. The followed-up was conducted 1 year after surgery, and the recurrence of gingival tumor was compared between 2 groups. RESULTS: No case was found shedding in the study. The total response rate of observation group was 97. 67％, which was significantly higher than 79. 07％ of control group (P＜0. 05). After treatment, the gingival related indexes of 2 groups were decreased significantly compared to before treatment, while quality scores of life were increased significantly compared to before treatment, observation group was significantly better than control group (P＜0. 05). No severe ADR or complication was found in 2 groups during treatment. Recurrence rate of gingival tumor in observation group was 2. 33％, which was significantly lower than 13. 95％ in control group 1 year after treatment (P＜0. 05). CONCLUSIONS: Compared with surgery alone, additional use of Cypate gingival fluid in periodontal basic therapy before surgery has better clinical efficacy and lower recurrence rate, with good safety.

OBJECTIVE: To investigate the effects of Kangfuxin solution combined with Chuangyangling and gelatin sponge on dry socket disease in patients with type 2 diabetes mellitus after the extraction of impacted teeth. METHODS: A total of 85 patients with impacted teeth and type 2 diabetes mellitus during Jan. 2016-Jan. 2017 were divided into group A (29 cases), B (25 cases), C (31 cases) according to random number table. All patients were treated with turbine drill method; in group A, Kangfuxin solution + Absorbable gelatin sponge was used to fill the wound site; in group B, Chuangyangling+compound preparation of Kangfuxin solution and gelatin sponge were used to fill the wound site; in group C, the wounds were filled with gauze but without any drugs. All patients received routine anti-infective treatment after surgery for 5-7 d. The time of impacted teeth extraction was observed in 3 groups; granulation tissue coverage integrity rate of tooth socket and the incidence of dry socket disease 7 d after surgery, the incidence of other postoperative complications, treatment cost, satisfaction scores were also comparod. RESULTS: There was no statistical significance in the time of impacted teeth extraction or treatment cost among 3 groups (P＞0. 05). Seven days after surgery, granulation tissue coverage integrity of tooth socket and satisfaction scores were in descending order: group B＞group A＞group C; the incidence of dry sockets were in ascending order: group B＜group A＜group C, with statistical significance (P＜0. 05). The incidence of other postoperative complications as bleeding, pain, swelling, limitation of mouth opening in group A and B were significantly lower than group C, with statistical significance (尸＜0. 05). There were no statistical significance between group A and B (P＞0. 05). CONCLUSIONS: Kangfuxin solution combined with Chuangyangling and gelatin sponge can effectively increase granulation tissue coverage integrity of tooth socket, and reduce the incidence of dry socket disease and other postoperative complications in patients with type 2 diabetes mellitus, but do not increase economic burden of patients and obtain high patient satisfaction.

[Objective] To identify the risk factors associated with uric acid stones, construct a nomogram model for predicting the occurrence of the disease, and evaluate its predictive performance. [Methods] A retrospective analysis was conducted on the general and clinical data of 876 patients who underwent surgical treatment for stones at the Department of Urology, the First Affiliated Hospital of Soochow University, during Jan.2020 and Dec.2022.Based on the analysis results of stone composition, the patients were divided into the uric acid stone group (n=82) and non-uric acid stone group (n=794). All patients were then randomly split into the training group (n=526) and validation group (n=350) in a ratio of 6∶4.The training group underwent LASSO regression, univariate, and multivariate logistic regression analyses to identify predictive factors associated with the occurrence of uric acid stones.Based on the factors, a nomogram model was constructed.The performance of the model was evaluated using the validation group data by comparing it with models from other research centers. [Results] LASSO regression, univariate, and multivariate logistic regression analyses revealed that cystatin C, urine pH, and stone CT values were predictive factors for uric acid stones.The area under the receiver operating characteristic curve (AUC) of the model was 0.968 for the training group and 0.956 for the validation group.Compared to other models, this model showed better predictive performance.The integrated discrimination improvement (IDI) and net reclassification index (NRI) in the training group were 0.420 0(95%CI: 0.328 2-0.511 8), P<0.001, and 0.484 2(95%CI: 0.321 3-0.647 2), P<0.001, respectively.In the validation group, the IDI and NRI were 0.405 9 (95%CI: 0.330 7-0.481 1), P<0.001, and 0.365 3 (95%CI: 0.211 6-0.519 0), P<0.001, respectively. [Conclusion] The nomogram model based on cystatin C, urine pH, and stone CT values can predict the occurrence of uric acid stones more accurately than other models, and can serve as a clinical supportive tool for the treatment and prevention of stone recurrence.

Objective:To explore the effect of chronic intermittent hypoxia(CIH)on learning and memory dysfunc-tion in rats,as well as the expression of high mobility group box-1(HMGB1)and nuclear transcription factor-KB(NF-κB)in the hippocampus region.Methods:The CIH rat model was established,and forty SD rats were randomly divid-ed into four groups:normoxia group,hypoxia for 4 weeks group(CIH4 group),hypoxia for 8 weeks group(CIH8 group),and hypoxia for 12 weeks group(CIH12 group).Morris water maze was used to assess the learning memory ability of rats,and immunohistochemistry and ELISA were used to detect the expression of HMGB1 and NF-κB in the hippocampus of rats.Results:Compared with the normoxia group,the CIH12 and CIH8 groups had longer escape la-tency,the number of crossing the platform and the residence time in the quadrant of the platform were significantly shortened,but there was no significant difference in the CIH4 group.Additionally,there was no significant expression of HMGB1 and NF-κB in the hippocampal region of the normoxia group,but little expression was observed in CIH4 group,and significantly expressed in CIH8 group and CIH12 group,and the expression of CIH12 group was significantly higher than that of CIH8 group.Conclusion:CIH can lead to a decline in learning and memory function in rats,and the longer time of intermittent hypoxia led to the more significant effect on their learning and memory function.In addi-tion,CIH also leads to increased expression levels of HMGB1 and NF-κB in the hippocampus region,and the expres-sion increased more significantly after hypoxia for 12 weeks,comparing to hypoxia for 8 weeks.

Chondrosarcoma original from the zygomatic arch is a very rare disease with high malignancy. Surgery is the main means of treatment at present for duo to its poor sensitivity to radiochemotherapy. We reported a young patient who was recovery well in a 4-years follow-up without radiochemotherapy after a total resection of the tumor.
Bone Neoplasms ; Chemoradiotherapy ; Chondrosarcoma ; Humans ; Self Concept ; Zygoma

ObjectiveTo investigate the effects of berbamine hydrochloride on sorafenib resistance in hepatocellular carcinoma cells and the underlying mechanisms. MethodThe sorafenib-resistant cell line SMMC-7721/S was selected by the concentration increment method starting at 1.25 μmol·L^-1 sorafenib. Both SMMC-7721 and SMMC-7721/S cells were treated with 0， 2.5， 5， 10， 15， 20 μmol·L^-1 sorafenib， and the cell counting kit-8 （CCK-8） assay was employed to determine the half maximal inhibitory concentration （IC₅₀） and calculate the resistance index （RI）. Western blot was conducted to compare the expression of proteins involved in autophagy and phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin （PI3K/Akt/mTOR） signaling pathway between SMMC-7721 and SMMC-7721/S cells. Furthermore， SMMC-7721/S cells were treated with 5 μmol·L^-1 berbamine hydrochloride alone or in combination with 2.5， 5， 10 μmol·L^-1 sorafenib， and the cell growth was assessed by the CCK-8 assay. In addition， SMMC-7721 and SMMC-7721/S cells were treated with 5 μmol·L^-1 berbamine hydrochloride alone or in combination with 5 μmol·L^-1 sorafenib， and the cell proliferation was examined by the colony formation assay. The immunofluorescence assays with Microtubule-associated protein 1 light chain 3 （LC3） and LysoTracker as probes were employed to assess the lysosomal acidification in SMMC-7721 cells treated with 5 μmol·L^-1 berbamine hydrochloride or 0.1 μmol·L^-1 autophagy inhibitor bafilomycin A1 （Baf）. Further， the expression of proteins involved in autophagy and PI3K/Akt/mTOR signaling pathway was determined by Western blot and compared between groups. ResultSorafenib showed the IC₅₀ of 9.56 mol·L^-1 （P<0.01） and 7.99 mol·L^-1 for SMMC-7721/S and SMMC-7721 cells， respectively， at 24 h. The resistance index （RI） of SMMC-7721/S for sorafenib was 1.20 （P<0.01）， which indicated mild resistance. Compared with SMMC-7721 cells， SMMC-7721/S cells exhibited up-regulated expression of p-mTOR， p-Akt， and LC3Ⅱ， down-regulated expression of p62 protein （P<0.01）， and unchanged Akt protein level. CCK-8 and colony formation assays demonstrated that the combination of berbamine hydrochloride and sorafenib exhibited a synergistic effect （Q>1.15）， with berbamine hydrochloride partially reversing the resistance of liver cancer cells to sorafenib. The immunofluorescence detection of LC3 revealed that berbamine hydrochloride and Baf significantly increased LC3 in SMMC-7721 cells. The detection with LysoTracker as the probe showed that berbamine hydrochloride inhibited the acidity of lysosomes in SMMC-7721 cells （P<0.01）， indicating the suppression of autophagy. Berbamine hydrochloride further enhanced the downregulation of p-mTOR and p-Akt protein levels and did not change the Akt protein level in SMMC-7721 cells exposed to sorafenib. Berbamine hydrochloride inhibited the increase in p-mTOR expression， down-regulated the p-Akt protein level， and did not change the total Akt protein level in the SMMC-7721/S cells exposed to sorafenib. ConclusionBerbamine hydrochloride can ameliorate the resistance of liver cancer cells to sorafenib by inhibiting cellular autophagy and the PI3K/Akt/mTOR signaling pathway.

Hutchinson-Gilford progeria syndrome (HGPS) and Werner syndrome (WS) are two of the best characterized human progeroid syndromes. HGPS is caused by a point mutation in lamin A (LMNA) gene, resulting in the production of a truncated protein product-progerin. WS is caused by mutations in WRN gene, encoding a loss-of-function RecQ DNA helicase. Here, by gene editing we created isogenic human embryonic stem cells (ESCs) with heterozygous (G608G/+) or homozygous (G608G/G608G) LMNA mutation and biallelic WRN knockout, for modeling HGPS and WS pathogenesis, respectively. While ESCs and endothelial cells (ECs) did not present any features of premature senescence, HGPS- and WS-mesenchymal stem cells (MSCs) showed aging-associated phenotypes with different kinetics. WS-MSCs had early-onset mild premature aging phenotypes while HGPS-MSCs exhibited late-onset acute premature aging characterisitcs. Taken together, our study compares and contrasts the distinct pathologies underpinning the two premature aging disorders, and provides reliable stem-cell based models to identify new therapeutic strategies for pathological and physiological aging.
Aging ; genetics ; physiology ; DNA Helicases ; genetics ; Human Embryonic Stem Cells ; metabolism ; physiology ; Humans ; Kinetics ; Lamin Type A ; genetics ; Mesenchymal Stem Cells ; metabolism ; physiology ; Mutation ; Progeria ; genetics ; physiopathology ; Werner Syndrome ; genetics ; physiopathology