OBJECTIVE:To standardize the operation of monitoring work of the adverse drug reaction in our hospital.METHODS:Based on the newly revised reporting and monitoring administration measures on adverse drug reaction,a detailed specification on a series of operations such as the finding,collection,analyzing,evaluation,feedback,sorting and quantization check of the drug adverse reaction(ADR)and the organization of the drug adverse reaction monitoring in our hospital was formulated.RESULTS:ADR reporting cases increased year by year in our hospital after the specification was formulated.CONCLUSION:This specification is feasible in operation,which can be put into practice well.

Along with the appearance and clinical use of rhEPO,a new approach has improved the treatment to tumor associated annemia. At present the clinical research confirmed the security and effectiveness of treating tumor related anaemia,but the effect of rhEPO Was not awgre of the generation of tumor cells.It effecls the treatable value of rhEPO on tumor related anaemia.So the effect of rhEPO on tumor associated anaemia has received great attention.

To observe the changes of artrial natriuretic peptide (ANP) and endothelin (ET) lev-els in patients with c0ngenital heart disease (CHD) and discuss their mechanism and clinical significance.Methods: The pulmonary pressure of the pulmonary hypertension (PH)group was higher than 2 kPa- Thepulmonary pressure of Non-PH group was n0rmal. Healthy children served as control group. Results: (1)Plasma ANP level of the PH group was apparently higher than that 0f the N0n-PH group and c0ntrolgroup. (2) Plasma ET levels in the peripheral vein, vena cava,right atrium, right ventricle, pulmonaryartery and left atrium of the PH group were particularly higher than that of the Non-PH group and c0ntr0lgroup, especially in the left atrium. C0nclusi0n: (l) CHD with PH induces the increase in cardiac v0lumeand pressure load, thus accelerates the release 0f ANP. It is actually a compensatory mechanism for the el-evation of endogenic ANP. (2) ET is a vasoconstrictor. ET owns the importance at PH pathogenesis andhas a certain clinical value in the diagn0sis 0f PH. (3) ET causes ANP releasing, then inhibits ET effectthrough negative feed. B0th of them are antag0nists each other.

Arsenic compounds are poisonous to organisms,which may induce chromosome aberration and gene mutation of cells.Arsenic compounds may also change the gene functions and cause carcinoma.After exposure to arsenic compounds,gene expressions in some cells were changed.The results of some molecular biology studies showed that both prokaryote and eukary-ote had responses to arsenic compounds at molecular levels.It will be important for unveiling the molecular mechanisms of ar-senic compoundseffects on cells and organisms and the arsenic-resistance of various cells and organism by studying the inter-actions between arsenic compounds and genes.In addition,it was recently found that arsenic trioxide could induce cell apoptosis by various cellular signal transduction pathways.

Objective To study the effects of arsenic compounds on metallothionein-3 gene expression in human normal hepatic cells. Methods cDNAs were cloned by SMART method. Bioinformatics was utilized to analyze the homologue, chromosomal localization, structure and encoding protein of the cloned gene and the trans-membrane information of the encoding protein. Metallothionein-3 gene expression level in L-02 cell line treated by arsenite was determined by cDNA microarrays. Results Metallothionein-3 cDNA gene was cloned and located in the chromosome 16q13. The results of bioinformatics analysis showed that the protein encoded by metallothionein-3 gene could cross the biologic membrane. Metallothionein-3 gene expression up-regulation in human normal hepatic L-02 cell line was found by cDNA microarrays in the early stage after the cells being exposed to arsenite. Conclusion The results of this study showed that the human metallothionein-3 gene was arsenic related gene and this gene might play a vital role in the detoxification metabolism of arsenic compounds at early stage.

Objective To detect the expression of EPOR in ovarian epithelial tumor. Study the biologic effect of rHuEPO to ovarian epithelial tumor cell H08910. Methods RT-PCR analyses were used to detect the expression of EPOR in ovarian epithelial tumor cell, FCM assay was used to detect rHuEPOs effect to ovarian epithelial tumor cell proliferation and inhibition ratio. Results There is EPOR expressions in ovarian epithelial tumor cell. rHuEPO could reduce ovarian epithelial tumor cell expressions (P <0.01). Conclusion The lower expression of EPOR was observed in ovarian epithelial tumor cell. Use rHuEPO couldn't promote the cancer's progression and bring negative effect in clinical practice. Further clinical research is required to prove it.

Objective To compare the differences of salivary alpha(α) amylase (sAA) activity and its activity ratio from the sali‐va before and after citric acid stimulation and approach the correlations among sAA activity determined by the methods of iodine‐starch ,Bernfeld and EPS‐G7 velocity respectively .Methods Ten saliva samples were collected from five healthy volunteers before and after citric acid stimulation .Their activities were determined three times by the three methods ,and the variation coefficient (CV) of sAA activity and activity ratio were calculated .Moreover ,correlation among sAA activities determined by the three meth‐ods were analyzed .Results The significant differences (P< 0 .05) were found in sAA activity and total CV from three determined methods ,and sAA activity and total CV by the method of EPS‐G7 velocity were minimum .There were no significant differences (P> 0 .05) in sAA activities ratio and its CV ;Significant correlation was found between sAA activity determined by random two of three methods(P < 0 .05) ,and their correlation coefficients were above 0 .96 .Conclusion The sAA activity data determined by three methods could be transformed each other by regression equation ,and determined precision by the method of EPS‐G7 velocity is highest ,and data processing method of sAA activity ratio could decrease differences among CV from three methods .

OBJECTIVE:To manufacture non-PVC multilayer extrusion film infusion bag and establish its quality standard METHODS:Polypropylene(PP),Polyethylene(PE) and Polyamide(PA) were mixed in proportion and extruded into film Infusion bag was prepared with the film Accroding to the demend of bulk infusion,its quality standard was established RESULTS:The infusion bag was good in quality,simple in preparation technic with no need of expensive facility CONCLUSI_ON:Non-PVC multilayer extrusion film infusion bag is worth further investigating

Objective To observe the effects of enriched environment on cognitive function in mice with sepsis-associated encephalopathy and to study the neuron PAS domain protein 4 (NPAS4)/brain deprive neurotrophic factor (BDNF)related mechanisms.Methods Sixty adult male mice were divided randomly into three groups:sham operation with standard environment group (group SS,n =12),cecal ligation and puncture with standard environment group (group CS,n =24),cecal ligation and puncture with enriched environment group (group CE,n =24).All mice were reared in standard environment or enriched environment for 28 days.The fear condition test was conducted on day 29 af-ter operation in mice.The change of NPAS4 and BDNF,the density of dendritic spine were detected by western blot or golgi staining.Results Compared with group SS,the context freezing time, NPAS4 and BDNF expression and the density of dendritic spine in hippocampus decreased significantly in group CS (P < 0.05).Compared with group CS,the context freezing time,NPAS4 and BDNF expression and the density of dendritic spine in hippocampus increased significantly in group CE (P <0.05).No significant difference was observed in the conditional freezing time among three groups.Conclusion Enriched environment can obviously improve cognitive function impairment induced by sepsis-associated encephalopathy,which may be related with up-regulated expression of NPAS4/BDNF,and promoted synaptic plasticity.

Objective To compare the curative effect of perindopril and metoprolol in the treatment of diabetic patients with asymptomatic cardiac insufficiency.Methods According to the method of random numbers, 152 diabetic patients with asymptomatic cardiac insufficiency were divided into the observation group and control group,with 76 cases in each group.The observation group was given with perindopril treatment,while control group was given with metoprolol treatment.The cardiac function index,glycosylated hemoglobin,BNP levels,the incidence of cardiac insufficiency and the occurrence of adverse drug reactions before and after treatment were compared.Results There were no significant differences in LVEF,Tei index and E/A between two groups before treatment (all P >0.05 ).Compared with before treatment,the cardiac function indexes of the two groups were significantly improved after treatment(t=5.96,4.83,4.91,10.22,5.68,5.18,all P<0.05),but the observation group were improved more significantly(t=5.64,4.67,5.03,all P<0.05).Before and after treatment,there was no significant difference in the levels of glycosylated hemoglobin(all P>0.05),and the difference between the two groups was not statistically significant(all P>0.05).The BNP levels of the observation group before and after the treatment were (515.43 ± 58.85)pg/mL and (214.43 ±32.42)pg/mL respectively,of which the control group were (513.94 ±59.11)pg/mL and (263.32 ±43.31)pg/mL respectively,and after treatment,the BNP levels of the two groups were significantly decreased(t=23.45,17.68,all P<0.05),of which the observation group decreased more obvious(t=6.97,P<0.05).The cardiac insufficiency incidence of the observation group was 3.95%,which was significantly lower than 14.47% of the control group(χ2 =5.03,P<0.05).The incidence of adverse drug reactions had no obvious differ-ences in both groups(χ2 =0.08,P>0.05).Conclusion Using perindopril in treatment of diabetic patients with asymptomatic heart function can obviously improve cardiac function,reduce the incidence of BNP and cardiac insuffi-ciency.It has the clinical curative effect and high safety.