AIM: To observe the effects of pioglitazone on the hearts of the rat' s models of ischemia-reperfusion in vivo. METHODS: Sprague-Dawley rats were randomly divided into two groups. One was the group of 30 min reperfusion, which was subdivided into sham (n =5), model (vehicle, n=6) and pioglitazone (3 mg?kg~(-1), n = 7) with 30 min ischemia followed by 30 min reperfusion to detect some data related to cardiac function and the area of myocardium infarction (MI). Another was the group of 2 h reperfusion, which was further subdivided into the sham (n = 5), model(vehicle, n=6), pioglitazone 0.3 mg?kg~(-1) (n = 6), 1 mg?kg~(-1) (n = 1) and 3 mg?kg~(-1) (n = 5) group. Then immunohistochemistry and in situ hybridization were performed to detect the expression of MMP-2 and PPAR? protein and mRNA. RESULTS: Compared with the model group, nec/aar after injecting pioglitazone decreased by 28% (P

Aim To observe the feature of pivanampeta (Piv) against ischemia-reperfusion injury with rat heart in vivo and to probe its molecular mechanism.Methods Sprague-Dawley rats were divided into two groups randomly.One was 30 min reperfusion group,which was subdivided into sham,model,vehicle,Piog and Piv with 30 min ischemia followed by 30 min reperfusion to detect some data related to cardiac function and the area of myocardium infarction.Another was 2 h reperfusion group,which was further subdivided into such sections as follows: sham,model,vehicle,Piog,Piv 3,6 and 9 mg?kg~(-1).Besides the sham group,vehicle,Piog,Piv and NS(model) were administered with Piv intravenously 30 min before occlusion.Then hearts were excised,paraffined and cut into 4 ?m.Immunohistochemistry,in situ hybridization,TUNEL and DNA agarose gel electrophoresis were performed to detect the expression of bax,bcl-2,caspase-3,MMP-2 and PPAR? protein and MMP-2,PPAR? mRNA.Results ① Compared with those of model,the ratio of nec/aar after Piv injection decreased 21%(P

Objective To understand serological detection results of syphilis among hospitalized patients in a gen-eral hospital,and provide new ideas for further prevention and treatment of syphilis. Methods Clinical data of inpa-tients with abnormal serological detection results for syphilis in a hospital from January 2012 to December 2013 were analyzed. Results A total of 164442 patients were admitted to a hospital from January 2012 to December 2013, 112576 of whom were performed syphilis serological screening,2048 cases were with abnormal results of serologi-cal detection for syphilis. The abnormal serological detection results were mainly in patients of 31~ and 41~ years (39.06% ),followed by patients of 51~,61~ years (34.42% ),and≥71 years (15.63% );unemployed people (34.08% ),farmers(23.05% ),and retirees(19.19% )were the main population with abnormal results. The abnor-mal serological detection results distributed in all 36 departments,the main departments were departments of respir-atory medicine(9.86% ),gynaecology(7.13% ),and cardiovascular internal medicine(6.88% ). Non-marital sex is the main route of transmission(56.79% );the main syphilis serological detection results were both positive for Treponemapallidum particle agglutination (TPPA)assay and rapid plasma reagin (RPR)(46.14% ),as well as TPPA positive and RPR negative (43.31% ). 860 (41.99% )patients were with latent syphilis. Conclusion The current status of patients with abnormal serological detection results of syphilis is not optimistic, mainly concentrated in patients of 31-50 years. It is necessary to strengthen publicity and education on prevention and treatment of syphilis,implement syphilis prevention and treatment policy,intensity syphilis screening,so as to pre-vent the epidemic and spread of syphilis.

Currently ischemic preconditioning is one of the most efficacious ways to treat ischemia reperfusion via triggering endogenously protective system. However, pharmacologic preconditioning has been produced due to the difficulty of performing ischemia preconditioning. Pharmacologic preconditioning, including both receptors and non-receptors, is actively investigated for the treatment of ischemia reperfusion.

Heat shock protein 70 (HSP70) is expressed differently in hepatocellular carcinoma (HCC) tissues. Since its expression and regulatory mechanism remain unclear, whether HSP70 can help with the early diagnosis, treatment, and prognostic evaluation of HCC has become a hot research topic. This article reviews the source of HSP70 and its family, abnormal expression of HSP70 in HCC, and its association with treatment methods and prognostic evaluation of HCC, in order to provide a reference for clinical diagnosis and treatment of HCC.