Thirty-six subclinical hypothyroidism patients with different pathogenic background were treated by levothyroxine. After treatment the levels of serum total cholesterol, low density lipoprotein and triglyceride were significantly lower than those before treatment (P

10 years group than those in ≤10 years group(p0 05).ConclusionIntravenous glucagon stimul ation test is of clinical value in distinguishing the difference of pancreas isl et function in type 2 diabetes mellitus.

Objective:To investigate the expression profile of Ly49A in a mouse GVHD model and the ex vivo expression alteration of it in T cells exposal to CsA, IL 4, and IL 15, respectively.Methods:Based on mouse aGVHD model, two color fluorescence flow cytometry was used to assay the expression of Ly49A on splenic and bone marrow CD3 +, CD4 + and CD8 + T cell subsets, before and after engraftment in different time interval. Cell culture was used to analyse in vitro Ly49A expression on splenic T cells treated with IL 4 and IL 15.Results:①In normal B6 and F1 strain, the frequencies of Ly49A + cells in BMCs , splenocytes and PMNCs remained at a low level and almost no, if any, such cells were detected in thymus; ②The proportions of CD3 +/Ly49A + andCD8 +/Ly49A + cells rose in early stage(7th,day) , tended to decline until 5 w post engraftment, and thereafter arose and exceed that pretreatment, which was down regulated in the presence of CsA; ③With or without ConA, the CD3 +/Ly49A + proportion were up modulated in purified CD3 +splenic cells, by IL 4 and IL 15 with no different effect demonstrated between those cytokines.Conclusion:KIR +T lymphocytes were most belong to peripheral lymphocytes. There exist a immunological tolerant tendency between grafts and host in semimatched transplant, and some cytokines and immunosuppressants can enhance the tolerance.

Based on the point of view that the human body is an organic whole and the fact that virus and body is a unity of contradiction,the interaction mechanism of virus and immunity in the research of the anti-HIV/AIDS Chinese materia medica is discussed.On the one hand,it can widen our thinking to find new target of anti-virus drugs.On the other hand,it can also provide scientific support for understanding the complex mechanics of the anti-HIV/AIDS Chinese materia medica.

This article elucidated the meaning of moving cupping method based on heaven-human-earth theory. The heaven-human-earth moving-cupping method has innovated the idea, operation, and application protocol of moving-cupping method. It also diversifies the moving routine and function, standardizes protocol, expands the area, and quantifies the operation. It endows the cupping therapy with a new method and idea. However, the action mechanism still expects further study.

Objective To observe the effect of fluvastatin on urinary albumin excretion rate(UAER)of microalbuminuria in type 2 diabetes patients.Methods Type 2 diabetes patients with mieroalbuminuria were randomly divided into fluvastatin goup(n=126)and control group(n=129).Fluvastatin group was given fluvastatin 40 mg each night.Follow up was conducted for 1.5 years.The UAER and glomerular filtration rate(GFR)as well as blood lipid level before and after therapy were compared.Results UAER in fluvastatin group was significantly lower than that in control group[(59.6±10.5)vs(87.5±12.3)mg/min,P＜0.05]before therapy[(104.4±25.2)vs(110.6±19.7)mg/min,P＜0.05],which was independent of its lowering-lipid effect.But GFR had no signifimicroalbuminuria which is independent of lowering-lipid effect in type 2 diabetic nephropathy patients and delay the progress of diabetic nephropathy.

~(131)I uptake by thyroid were assayed in 64 patients with Graves′ disease (GD) and 69 patients with non-GD hyperthyroidism. GD group had higher rate of ~(131)I uptake than non-GD group at 2, 6 and 24 h, and there was no overlap between the two groups at these 3 time points.~(131)I uptake at 2 and 6 h could differentiate GD fromlow ~(131)I uptakehyperthyroidism.

Objective:To explore the significance of next-generation sequencing to detect gene mutations for diagnosis of patients with unexplained pancytopenia.Methods:The next-generation sequencing was used to detect 32 common hematological tumor gene mutations in 113 patients with unexplained pancytopenia in Huazhong University of Science and Technology Union Shenzhen Hospital from February 2016 to February 2019.Results:The next-generation sequencing results showed that of the 32 genes tested in 113 patients, 69 mutations occurred in 25 genes. Among them, 105 patients (92.9%) carried at least 1 gene mutation, and the median number of mutations was 2 (1-5). Fifty-one patients were diagnosed with myelodysplastic syndrome (MDS), and the related mutant genes were detected; 62 patients were diagnosed with atypical aplastic anemia or cytopenia of unknown significance. The total effective rate of treatment for 113 patients was 65.5% (74/113).Conclusion:The next-generation sequencing helps to diagnose patients with unexplained pancytopenia, and provides targeted strategies for finding new treatments and prolonging the overall survival of patients.

Objective To study the constituent ratio of thyroid nodules in inland Changchun area and compare it with the data in the coastal Shanghai area Methods A retrospective analysis was made on 9216 cases of thyroid nodules proved by pathology in a period of 39 years in Changchun and on 7018 cases in a period of 37 years in Shanghai, to compare the difference of the constituent ratio of thyroid cyst, nodular goiter, thyroid adenoma and thyroid carcinoma in these two areas Results The constituent ratio of thyroid cyst in Changchun and Shanghai area was 1 83% and 8 68%, that of nodular goiter was 48 31% and 18 62%, that of thyroid adenoma was 39 30% and 49 94%, and that of thyroid carcinoma was 10 55% and 22 76%, respectively There was a significant difference in the constituent ratio of thyroid nodules between the two areas Conclusion The difference is significant in the constituent ratio of thyroid nodules between China inland and coastal areas

Objective To find new way to reduce non-recurrent laryngeal nerve (NRLN) injuries by applying intraoperative neuromonitoring(IONM) to identify NRLN in thyroidectomy. Methods Records of 279 patients who underwent complex thyroidectomy by applying IONM to identify and monitor RLN from Mar. 2009 to Jan. 2010 were veviewed. We proposed the skills to identify and monitor NRLN and predict RLN varition through exploring vagus nerve and RLN before RLN dissection. Results 6 cases NRLN located on the right side were all accurately identified by IONM, thus no injury of NRLN occurred during thyroid operations. Conclusions NRLN is difficult to be predicted preoperatively and identified by naked eyes. The application of IONM to predict, identify and monitor NRLN could remarkably reduce the possibility of NRLN injury.