1.RESEARCH ADVANCES ON STRUCTURES AND FUNCTIONS OF BACULOVIRUS CHITINASE GENES
Microbiology 1992;0(02):-
The chitinase gene of baculovirus is an unnecessary and high conservative gene Chitinase is expressed in the late phase virus replication in insect cells There are signal peptide in its N termini, chitinases active motifs in its central section and ER attaching motifs of chitinase in its C termini Baculoviral chitinase possesses both endo and exo chitinase activities, which can hydrolyze inherent chitin in insects body Chitinase is necessary for liquefaction of the virus infected host insect, and it may also serve as a molecular chaperone for pro V Cath Perhaps Baculoviral chitinase has same ancestors with bacteriums chitinase
2.The effects of early rehabilitation therapy in mechanically ventilated patients
Zehua DONG ; Bangxu YU ; Yunbo SUN ; Wei FANG ; Lei LI
Chinese Journal of Emergency Medicine 2013;22(10):1153-1156
Objective To investigate the effects of early rehabilitation therapy in mechanically ventilated ICU in patients.Methods A randomized controlled trial was carried out.Sixty mechanically ventilated patients,with tracheal intubation or tracheostomy more than 48 h and less than 72 h,were admitted to the intensive care unit (ICU) of the Affiliated Hospital of Medical College Qingdao University from May 2010 to May 2012.These patients were randomly (random number) divided into two groups,the rehabilitation group and the control group,30 patients in each group.In rehabilitation group,rehabilitation therapy was taken twice daily,and the training time and intensity was adjusted according to the condition of the patients.Early rehabilitation therapy included head up actively,transfer from the supine to sit,out of bed,transfer to a chair,standing bedside bed and walking bedside bed.The patient' s body mass index,days to first out of bed,duration of mechanical ventilation,ICU stay,APACHE Ⅱ score,highest FiO2,lowest PaO2/FiO2 and hospital mortality of patients were compared between rehabilitation group and control group.The differences between the groups were compared using t test.Results There was no significant difference in body mass index,APACHE Ⅱ score,highest FiO2,lowest PaO2/FiO2 and hospital mortality between rehabilitation group and control group (P > 0.05).Patients in the rehabilitation group had shorter first out of bed time [(3.8 ± 1.2) d vs.(14.9 ±4.7) d; P =0.00],duration of mechanical ventilation [(5.6±2.1) dvs.(12.7±4.1) d; P=0.005] andICUstay [(7.3±2.8) dvs.(15.2±4.5) d;P =0.01] compared with control group.Conclusions Early rehabilitation therapy was safe and effective in improving the outcomes of mechanical ventilation patient.
3.Application of recombinant human growth hormone in improving difficult weaning
Zehua ZHANG ; Fang WANG ; Dehua KONG ; Jun LIU ; Sizhao LI ; Chao YAN ; Chao ZHOU
Chinese Journal of Primary Medicine and Pharmacy 2014;(15):2278-2279,2280
Objective To observe the applicated value of recombinant human growth hormone ( r-hGH) ther-apy in improving difficult weaning .Methods 40 patients with difficult weaning were randomly divided into the treat-ment group (20 cases) and control group (20 cases).The control group received routine ICU treatment .The treat-ment group was given the conventional treatment and subcutaneous injection of r -hGH 8IU,1 time a day,a total of 14 days.The serum total protein,albumin,oxygenation index,peak of blood glucose,independent tidal volume changes were observed before and after treatment , the success rate and mortality rate were compared .Results After treat-ment,the total protein,albumin,tidal volume of the treatment group were (68.1 ±13.4) g/L,(40.8 ±6.0) g/L, (432 ±57)mL,which were significantly higher than those before treatment [(52.3 ±12.5)g/L,(28.4 ±4.5)g/L, (278 ±54)mL](t=3.856,7.394,8.771,all P<0.05).While after treatment,the total protein,albumin,tidal vol-ume in the control group were (58.4 ±14.2)g/L,(31.0 ±5.8)g/L,(398 ±48)mL,compared with before treatment (53.2 ±13.3)g/L,(28.1 ±4.2)g/L,(271 ±49)mL,the differences were not statistically significant (all P>0.05).The success rate was 85% in the treatment group,which was higher than 50% in the control group(χ2 =4.103,P<0.05).In the treatment group,3 cases died,in the control group 9 cases died,there was statistically signif-icant difference between the two groups (χ2 =4.286,P<0.05).Conclusion r-hGH can improve the nutritional sta-tus of critically ill patients ,increase the strength of the respiratory muscles ,improve lung function ,help to improve the weaning rate .
4.A comparative study of deglutition function of amyotrophic lateral sclerosis patients with or without diabetes mellitus type Ⅱ
Zehua FANG ; Yu ZHENG ; Biying YANG ; Zhijian ZHOU ; Baoxin DU
Chinese Journal of Neuromedicine 2016;15(12):1255-1259
Objective To explore the association between diabetes mellitus type Ⅱ (DM) and deglutition function of amyotrophic lateral sclerosis (ALS) patients.Methods Seventy-five ALS patients older than 45 years and admitted to our hospital from August 2008 to November 2015 were selected into this study;67 of them were without DM and 8 were with DM.Water swallow test,amyotrophic lateral sclerosis severity scale-swallow (s-ALSSS),amyotrophic lateral sclerosis functional rating scale (ALSFR-R) and videofluoroscopic swallow study (VFS) were performed to evaluate the deglutition functions of these ALS patients with or without DM,and the results of the two groups were compared.Results (1) Patients with DM had significantly higher s-ALSSS scores,ingurgitation part in ALSFRS-R scores and in parts of VFS scores,such as transportation to pharyngeal,pharyngeal transit,flow into pharyngeal before reflexion,epiglottic vallecula residue,and piriform sinus residue than patients without DM (8.88±1.34 vs.7.54±1.47,3.50±0.54 vs.2.96±0.77,2.88±0.35 vs.2.16±0.69,2.75±0.46 vs.2.09±0.69,2.88±0.35 vs.2.42±0.56,2.88±0.35 vs.2.39±0.58,P<0.05).(2) Scores of Kubota drinking test,ALSFRS-R,VGF (including oral phase,pharyngeal phase and aspiration degree) in patients without DM were 2.15±1.12,7.18±1.41(1.78±0.69,1.69±0.60 and 3.72±0.65),and those in patients with DM were 1.88±1.34,8.00±0.93(2.13±0.64,2.00±0.53 and 4.00±0.00);no significant differences were noted between the two groups (P>0.05),but there was a trend showing that DM patients had higher scores in these evaluations.Conclusion As compared with ALS patients without DM,ALS patients with DM get more mildly impaired deglutition function.
5.Association of programmed cell death 1 (PDCD1) gene polymorphisms with colorectal cancer among Han Chinese population.
Yuancun ZHAO ; ; Zhangj@scu.edu.cn. ; Zhigang MAO ; Hua PANG ; Xiaohong ZHAO ; Shu ZHANG ; Zehua GAO ; Yiwen YANG ; Ting FANG ; Qizhao MA ; Xiaodan MA ; Yufang WANG ; Ji ZHANG
Chinese Journal of Medical Genetics 2018;35(2):219-223
OBJECTIVETo assess the association of programmed cell death 1 (PDCD1) gene polymorphisms with the susceptibility and/or progression of colorectal cancer.
METHODSA hospital-based case-control study was carried out, which recruited 426 colorectal cancer patients and 500 healthy individuals. Five single nucleotide polymorphisms, namely rs36084323, rs11568821, rs2227981, rs2227982 and rs10204525, were selected for the study and genotyped with a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.
RESULTSThe G allele of rs36084323 under a dominant model was associated with increased risk of advanced TNM staging of colorectal cancer progression (OR=1.59, 95%CI=1.02-2.48). Haplotypes G-G-C-T-A and A-G-C-C-G of the rs36084323, rs11568821, rs2227981, rs2227982, and rs10204525 were negatively associated with the occurrence of colorectal cancer.
CONCLUSIONThe G allele of rs36084323 is associated with increased risk of advanced TNM staging of colorectal cancer. Conversely, the incidence of colorectal cancer is negatively associated with the haplotypes G-G-C-T-A and A-G-C-C-G of rs36084323, rs11568821, rs2227981, rs2227982, and rs10204525.
Asian Continental Ancestry Group ; genetics ; Case-Control Studies ; China ; ethnology ; Colorectal Neoplasms ; genetics ; pathology ; Genetic Predisposition to Disease ; Haplotypes ; Humans ; Neoplasm Staging ; Polymorphism, Single Nucleotide ; Programmed Cell Death 1 Receptor ; genetics
6.Segmentation of prostate region in magnetic resonance images based on improved V-Net.
Mingyuan GAO ; Shiju YAN ; Chengli SONG ; Zehua ZHU ; Erze XIE ; Boya FANG
Journal of Biomedical Engineering 2023;40(2):226-233
Magnetic resonance (MR) imaging is an important tool for prostate cancer diagnosis, and accurate segmentation of MR prostate regions by computer-aided diagnostic techniques is important for the diagnosis of prostate cancer. In this paper, we propose an improved end-to-end three-dimensional image segmentation network using a deep learning approach to the traditional V-Net network (V-Net) network in order to provide more accurate image segmentation results. Firstly, we fused the soft attention mechanism into the traditional V-Net's jump connection, and combined short jump connection and small convolutional kernel to further improve the network segmentation accuracy. Then the prostate region was segmented using the Prostate MR Image Segmentation 2012 (PROMISE 12) challenge dataset, and the model was evaluated using the dice similarity coefficient (DSC) and Hausdorff distance (HD). The DSC and HD values of the segmented model could reach 0.903 and 3.912 mm, respectively. The experimental results show that the algorithm in this paper can provide more accurate three-dimensional segmentation results, which can accurately and efficiently segment prostate MR images and provide a reliable basis for clinical diagnosis and treatment.
Male
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Humans
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Prostate/diagnostic imaging*
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Image Processing, Computer-Assisted/methods*
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Magnetic Resonance Imaging/methods*
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Imaging, Three-Dimensional/methods*
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Prostatic Neoplasms/diagnostic imaging*
7. Serum antibodies against norovirus GⅠ.1 and GⅡ.4 in populations in central and eastern China
Zibo HAN ; Yun KANG ; Zehua LEI ; Runyu YUAN ; Zhenni WEI ; Shuo SHEN ; Zhenlu SUN ; Fang TANG ; Junwei HOU ; Lifang DU ; Jing ZHANG ; Qiming LI
Chinese Journal of Microbiology and Immunology 2019;39(11):840-847
Objective:
To detect norovirus (NoV) GⅠ.1- and GⅡ.4-specific IgG, IgA and histo-blood group antigen (HBGA)-blocking antibodies in healthy populations of all age groups in China for better understanding the epidemiological features of norovirus in China from a serological point of view and providing basic data for vaccine development and clinical trial design.
Methods:
Indirect ELISA and HBGA-blocking assay were used to detect NoV-specific IgG, IgA and HBGA-blocking antibodies in serum samples collected from healthy natural populations (