Objective:To study the effects of recombinant human vascular endothelial growth factor on proliferation and differentiation of rat osteoblasts. Methods: Cell culturing, light microscop, MTT assay and PNPP assay were performed to observe the effects of rhVEGF on proliferation and differentiation of the cells. Results: rhVEGF could stimulate osteoblasts proliferation and differentiation. The maximal effect on proliferation was observed at a concentration of 3.125 ng/ml on the third day. ALP activities in osteoblasts were increased most significantly at a concentration of 12.5 ng/ml. Conclusion: rhVEGF may not only induce angiogenesis to facilitate bone formation, but also stimulate this process in a direct way by inducing proliferation and differentiation of osteoblasts.

Objective To evaluate the diagnostic value of radionuclide salivagram in children with pulmonary aspiration.Methods From March 2012 to June 2015,a total of 62 patients (37 males,25 females;age range:2 d-14 years) with suspected pediatric aspiration pneumonia were enrolled in this retrospective study.All patients underwent gastroesophageal reflux (GER) imaging and(or) radionuclide salivagram.Detection rate of pulmonary aspiration by the two imaging techniques was compared with x2 test.Results Of 62 patients,14 were diagnosed as pulmonary aspiration,including 1 detected by GER imaging,and 13 detected by salivagram.The detection rate for pulmonary aspiration by radionuclide salivagram (26.0％,13/50) was significantly higher than that by GER imaging (3.1％,1/32;x2=7.211,P＜0.05).Eight of the 13 cases with pulmonary aspiration diagnosed by radionuclide salivagram underwent upper gastrointestinal radiography,and 5 cases had visible contrast agent in the airway.Conclusion Radionuclide salivagram has a higher detection rate for pulmonary aspiration compared to GER imaging,and has good concordance with the traditional upper gastrointestinal radiography.

Objective To develop a novel stress fracture animal model system based on dynamic cyclic mechanical loading.Methods Ulnae and tibiae of rats were respectively fixed using differently shaped fixtures (including a fixed holder and a movable holder).The axial mechanical loading was applied via a linear actuator based on LabVIEW control program.During the loading process,a load cell and a laser displacement transducer were used to detect the force and displacement changes in the limbs,respectively.The two signals were sampled at real time by PC-based LabVIEW data acquisition program.A compressive loading test (peak force of 50 N) was conducted to examine the consistency and reliability of cyclic loads after completing the loading system.The loading system and an ElectroForce 3220 mechanical testing machine were respectively used to measure and compare the Young's modulus of a standard ultra-high molecular weight polyethylene (UHMWPE)cylinder to calibrate the loading system.Results The compressive loading test (peak force of 50 N) demonstrated that the mechanical loading system was able to apply consistent loads with controllable intensity and time.The calibration experiment indicated the accuracy of the loading system.Conclusion The novel mechanical loading device has characteristics of precision,scientificity and reliability,and it is approaching the real development situation of stress fracture,which may provide a reliable experimental base for exploring the precautionary measures of stress fracture.

Objective Cisplatin(DDP)is widely used in the chemotherapy of lung cancer. However, cisplatin resistance represents a major obstacle in its effective treatment. Our preliminary work has demonstrated that inactivated Sendai virus(HVJ-E)shows that it induces apoptosis in murine melanoma cells(B16)and obviously inhibites the tumor growth in tumor-bearing BALB/c nude mice. This study aims to investigate whether inactivated HVJ-E has an effect of inducing apoptosis in cisplatin-resistant A549/DDP lung adenocarcinoma cells in vitro and in vivo. Methods HVJ-E and A549/DDP cells were co-cultured in vitro,and the effect of HVJ-E on the apoptosis in A549/DDP cells was detected by flow cytometry. In addition,HVJ-E was injected into the tumor in vivo, and its oncolytic effect was observed by TUNEL assay of tissue sections and measurement of tumor size. Results After co-cultured with HVJ-E for 12 h,24 h and 36 h, the apoptosis rate of A549/DDP cells in late stage detected by flow cytometry was 7.7%, 12.6% and 18.9%,respectively,showing a significant difference between 12 h and 24 h, and between 24 h and 36 h. TUNEL assay showed that there was more apoptosis in tumor cells in vivo in the experimental group than in the control group. Meanwhile, intratumoral injection of HVJ-E induced a significantly smaller tumor volume in the experimental group compared with the control group(P ﹤ 0.05). Conclusions Our findings indicate that inactivated HVJ-E can induce apoptosis in A549/DDP cells both in vitro and in vivo, and intratumoral injection of inactivated Sendai virus significantly reduces the tumor growth in vivo.

Pyroptosis is a form of new programmed cell death which is dependent on Caspase-1 in recent years.When it' s stimulated by various dangerous signals from hepatic ischemia-reperfusion injury,the intracellular pattern recognition receptors are assembled into inflammasomes and Caspase-1 which was transformed into active form.Activated Caspase-1 promotes the maturation and secretion of pro-inflammatory cytokines IL-1β and IL-18,initiates the innate immunity rapidly and then induces severe inflammatory reaction.In addition,Caspase-1 can also cleave Gasdermin D and release its N-terminal domain triggering pyroptosis.Many studies showed that pyroptosis play a crucial role in hepatic ischemia-reperfusion injury.In this review,we discussed the activation mechanism and research progress of pyroptosis in hepatic ischemia-reperfusion injury.

Objective:In the context of the aging trend of China's population,it aims to analyze the trend of elderly disabled population growth in various regions and calculate the potential payment pressure of long-term care insurance for the disabled population,thereby providing policy implications for China's long-term care insurance system.Methods:A model for estimating the amount of expenditure on long-term care insurance fund was constructed,using the population age structure of each province in China from 2025 to 2060 obtained from the random forest model,and combining multi-source data such as the disability rate of the elderly population and the care cost of disabled elderly people to predict the number of elderly disabled people and the payment amount of long-term care insurance in each province in the future.Results:The aging trend in China will continue to intensify and exhibit a significant structural feature.The peak of the aging ratio in northeastern regions such as Inner Mongolia,Heilongjiang,Jilin,and Liaoning will come earliest,while the number of elderly people with disabilities in provinces with large populations such as Shandong,Sichuan,and Henan are relatively larger.Guangdong will have the largest number of disabled elderly people by 2055.Conclusion:It is suggested that the long-term care insurance system should be steadily advanced,with national-level support for the construction of the system in Northeast China and populous provinces.

Objective:In the context of the aging trend of China's population,it aims to analyze the trend of elderly disabled population growth in various regions and calculate the potential payment pressure of long-term care insurance for the disabled population,thereby providing policy implications for China's long-term care insurance system.Methods:A model for estimating the amount of expenditure on long-term care insurance fund was constructed,using the population age structure of each province in China from 2025 to 2060 obtained from the random forest model,and combining multi-source data such as the disability rate of the elderly population and the care cost of disabled elderly people to predict the number of elderly disabled people and the payment amount of long-term care insurance in each province in the future.Results:The aging trend in China will continue to intensify and exhibit a significant structural feature.The peak of the aging ratio in northeastern regions such as Inner Mongolia,Heilongjiang,Jilin,and Liaoning will come earliest,while the number of elderly people with disabilities in provinces with large populations such as Shandong,Sichuan,and Henan are relatively larger.Guangdong will have the largest number of disabled elderly people by 2055.Conclusion:It is suggested that the long-term care insurance system should be steadily advanced,with national-level support for the construction of the system in Northeast China and populous provinces.

Objective:In the context of the aging trend of China's population,it aims to analyze the trend of elderly disabled population growth in various regions and calculate the potential payment pressure of long-term care insurance for the disabled population,thereby providing policy implications for China's long-term care insurance system.Methods:A model for estimating the amount of expenditure on long-term care insurance fund was constructed,using the population age structure of each province in China from 2025 to 2060 obtained from the random forest model,and combining multi-source data such as the disability rate of the elderly population and the care cost of disabled elderly people to predict the number of elderly disabled people and the payment amount of long-term care insurance in each province in the future.Results:The aging trend in China will continue to intensify and exhibit a significant structural feature.The peak of the aging ratio in northeastern regions such as Inner Mongolia,Heilongjiang,Jilin,and Liaoning will come earliest,while the number of elderly people with disabilities in provinces with large populations such as Shandong,Sichuan,and Henan are relatively larger.Guangdong will have the largest number of disabled elderly people by 2055.Conclusion:It is suggested that the long-term care insurance system should be steadily advanced,with national-level support for the construction of the system in Northeast China and populous provinces.

Objective:In the context of the aging trend of China's population,it aims to analyze the trend of elderly disabled population growth in various regions and calculate the potential payment pressure of long-term care insurance for the disabled population,thereby providing policy implications for China's long-term care insurance system.Methods:A model for estimating the amount of expenditure on long-term care insurance fund was constructed,using the population age structure of each province in China from 2025 to 2060 obtained from the random forest model,and combining multi-source data such as the disability rate of the elderly population and the care cost of disabled elderly people to predict the number of elderly disabled people and the payment amount of long-term care insurance in each province in the future.Results:The aging trend in China will continue to intensify and exhibit a significant structural feature.The peak of the aging ratio in northeastern regions such as Inner Mongolia,Heilongjiang,Jilin,and Liaoning will come earliest,while the number of elderly people with disabilities in provinces with large populations such as Shandong,Sichuan,and Henan are relatively larger.Guangdong will have the largest number of disabled elderly people by 2055.Conclusion:It is suggested that the long-term care insurance system should be steadily advanced,with national-level support for the construction of the system in Northeast China and populous provinces.

Objective:In the context of the aging trend of China's population,it aims to analyze the trend of elderly disabled population growth in various regions and calculate the potential payment pressure of long-term care insurance for the disabled population,thereby providing policy implications for China's long-term care insurance system.Methods:A model for estimating the amount of expenditure on long-term care insurance fund was constructed,using the population age structure of each province in China from 2025 to 2060 obtained from the random forest model,and combining multi-source data such as the disability rate of the elderly population and the care cost of disabled elderly people to predict the number of elderly disabled people and the payment amount of long-term care insurance in each province in the future.Results:The aging trend in China will continue to intensify and exhibit a significant structural feature.The peak of the aging ratio in northeastern regions such as Inner Mongolia,Heilongjiang,Jilin,and Liaoning will come earliest,while the number of elderly people with disabilities in provinces with large populations such as Shandong,Sichuan,and Henan are relatively larger.Guangdong will have the largest number of disabled elderly people by 2055.Conclusion:It is suggested that the long-term care insurance system should be steadily advanced,with national-level support for the construction of the system in Northeast China and populous provinces.