OBJECTIVE:To prepare Duzhong pingya dispersible tablets and establish their quality control.METHODS:The ratios of the excipients were optimized with disintegrating time,dispersible uniformity and compressibility as indexes.The Duzhong pingya dispersible tablets were prepared by trituration,and the content of chlorogenic acid was determined by HPLC.RESULTS:The optimized ratios of the excipients were as follows:manicol 60 g,microcry stalline cellulose 40 g,low-substituted hydroxypropyl cellulose 30 g,gum arabic 20 g,crospovidone 4 g,magnesium stearate 2 g,edulcorant 2 g,and plasdone vis quantum.The optimized Duzhong pingya dispersible tablets were brown in color and up to the standard in dispersible uniformity.The linear range of chlorogenic acid was 1.882～18.820 ?g(r=0.999 9)and its average recovery rate was 99.2%(RSD=1.63%,n=6).CONCLUSION:The ratios of the excipients were up to the standard required for the preparation technology,and the established quality standard is applicable for the quality control of Duzhong pingya dispersible tablets.

10pg/ml and IL-8≥70 pg/ml as the critical value,PCT and IL-6 levels had diagnostic sensitivities of 90.6% and 75.0%,and specificities of 83.3% and 77.7%,respectively.The combination of PCT and IL-6 had a sensitivity of 81.2%,and specificity of 94.2%.The positive predictive value was of 96.2% and the negative predictive value of 73.0%.CONCLUSIONS Serum PCT appears to be a promising indicator in differentiating viral infection from bacterial gastroenteritis,but the combination of PCT and IL-6 has a more clinical value.

OBJECTIVE To analyze in vitro activity of ertapenem and other antibiotics against clinical bacterial isolates of ESBLs-producing Escherichia coli and Klebsiella pneumoniae.METHODS E.coli and K.pneumoniae were identified by WalkAway40 with 21CN.ESBLs-producing isolates confirmed accordance with the CLSI guidelines.E-test strips were used for determining the minimum inhibitory concentration(MIC) of ertapenem,while disk diffusion test was performed on each isolate using 10 ?g ertapenem disc.RESULTS ESBLs-producing strains were isolated mainly from sputum,urine,secretion and blood,most of them were from nephrology,neurosurgery,Respiratory department and ICU.High resistance rates were shown for both E.coli and K.pneumoniae against cefepime,ciprofloxacin(63.42%),SMZco(61.78%),tobramycin(45.22%)and gentamicin(61.14%).Most K.pneumoniae and E.coli isolates were susceptible to amikacin(78.70%) and piperacillin/tazobactam(81.30%).Tmipenem showed 100.00% of activity the isolates.Ertapenem showed activity against 100.00% ESBLs-producing E.coli,one ESBLs-producing K.pneumoniae isolate showed ertapenem resistance and another one intermediate,the ratio of susceptibility was 99.40%,ertapenem had MIC at which 50.00% and 90.00% of the isolates were inhibited of 0.032 ?g /ml and 1.0 ?g /ml for ESBLs-producing organisms respectively.CONCLUSIONS Ertapenem is less active to ESBLs-producing organisms then imipenem.Ertapenem resistance appears to be the most sensitive indicator for detection of KPCs.

Through literature method,expert consultation method,K-means algorithm and empirical analysis method,the paper analyzes the methods for evaluation on the layout of first-aid sites,discusses the indexes for scientific evaluation on the rationality of layout of first-aid sites,conducts visualized and practical calculation based on the first-aid related data of Wuhan city in 2015,verifies the rationality and operability of the evaluation indexes,and provides reference for overall allocation of first-aid resources and rational layout of first-aid sites.

Objective To investigate the effects of the interaction between human hepatoma cells and hepatic stellate cells on their growth state,and study its role of interaction on the progression of hepatocellular carcinoma.Methods Human hepatoma cell line HepG2 and hepatic stellate cell line hepatic stallate cells (HSC)-T6 were used and the methods including methyl thiazolyl tetrazolium (MTT) assay,flow cytometry (FCM) analysis,immunohistochemistry,and electron microscopy were employed in this experiment.The effects of conditioned medium (CM) of HepG2 on the activation and proliferation of HSC were explored.The effects of activated HSC CM on HepG2 proliferation were investigated.The uhrastructural changes of the two co-cultured cells were observed.Results MTT assay result showed that HepG2/HSC CM could promote HSC/HepG2 proliferation.FCM result demonstrated that HepG2/HSC CM could influence the cell cycle distribution in HSC/HepG2.Immunohistochemistry exhibited that after the treatment of HepG2/HSC CM,the expression ofα-smooth muscle actin (α-SMA) in HSC and proliferating cell nuclear antigen (PCNA) in HepG2 were increased.When HepG2 and HSC were co-cultured,the ultrastructure of HSC displayed an activated feature.Conclusions HepG2 cells can induce the activation and proliferation of HSC,and the activated HSC can also stimulate the proliferation of HepG2.Interaction between hepatoma cells and hepatic stellate cells may play an important role in the progression of hepatocellular carcinoma.

Objective To explore the expressions and distributions of glial cell line -derived neurotrophic factor (GDNF)and itstyrosine kinase receptor RET in the terminal rectums of fetal rats with congenital anorectal malfor-mations (ARM)at different gestationalage,and to explore their effects on the enteric nervous system in the terminal rectum of ARMfetal rats.Methods Thirty -five SD pregnancy rats were divided into a saline group (n =1 0)and an ethylenethiourea experiment group (n =25)by simple randomized study.The fetal rats were removed from the pregnant rats at the gestational 1 6 d,1 8 d and 20 d.The fetal rats were divided into the saline control group,the ethylenethiourea control group (fetal rats without ARM)and the ethylenethiourea malformation group (ARM fetal rats)by the naked eye and dissecting microscope.HE staining was used to observe the morphology and the intestinal ganglion cells in the terminal rectum were counted.The immunohistochemical staining and Western blot methods were used to observe the distributions of GDNF and RET in the rectum at the gestational 1 6 d,1 8 d and 20 d.The quantitative real -time poly-merase chain reaction (qRT -PCR)was used to detect the expression of GDNF mRNA in the fetal rats in the terminal rectum at the gestational 1 6 d,1 8 d and 20 d.Results HE staining:the development of anorectal terminal in 3 groups of fetal rats was unclear at the gestational 1 6 d.A small amount of scattered nerve plexuses were observed in the muscu-lar layer.The nuclei were small and sparse.The axons and cytoplasms were less.The serosal layer,muscular layer,sub-mucosa,mucosal layer and glands in the terminal rectum were gradually clear in the saline control group and the ethyle-nethiourea control group at the gestational 1 8 d and 20 d.The intermuscular submucosal nerve plexuses increased gra-dually (1 1 .400 ±3.1 34 and 1 1 .200 ±3.425 at the gestational 1 8 d;66.1 00 ±4.954 and 67.600 ±5.481 at the gesta-tional 20 d).While,the layer was unclear in the ethylenethiourea malformation group and the nerve plexus was less (7.800 ±1 .989 at the gestational 1 8 d,and 25.200 ±3.048 at the gestational 20 d),and the difference was statistical-ly significant compared with 2 control groups (F =7.591 ,271 .833,all P <0.05).Immunohistochemistry satning:the expressions of GDNF and RET in all layers of the intestinal wall in the 3 groups of fetal rats were unclear at the gesta-tional 1 6 d and only a few positive cells were observed.The GDNF and RET were expressed in the mucosal layer and submucosa of the terminal rectum as well as intermuscular nerve plexus in the saline control group and the ethylene-thioured control group at the gestational 1 8 d and 20 d.With the continuous development of the embryo,their expression intensities were gradually increased.The expressions of GDNF and RET positive cells were decreased gradually in the ethylenethiourea malformation group.The difference was significant statistically compared with 2 control groups (all P <0.05).qRT -PCR:the expressions of GDNF mRNA showed no statistical difference among 3 groups at the gestational 1 6 d (P >0.05);the expressions of GDNF and RET protein were 1 03.624 ±27.533 and 1 05.1 84 ±1 9.634 at the ges-tational 1 8 d;1 51 .496 ±33.622 and 1 50.738 ±21 .423 at the gestational 20 d in 2 control groups.Compared with the ethylenethiourea malformation group (79.1 69 ±1 1 .697 at the gestational 1 8 d;94.873 ±1 1 .309 at the gestational 20 d),and the difference were statistically significant (all P <0.05).Conclusions The expressions of GDNF and its tyrosine kinase receptor RET had a certain temporal correlation in the terminal rectum of normal fetal rats at different gestational ages and ARM.Moreover,the abnormal expressions of GDNF and its tyrosine kinase receptor RET in the dis-tal rectum of ARMfetal rats can affect the development of enteric nervous system.

Objective To observe the effects of acupuncture therapy on triglyceride (TG) content of quadriceps femoris in the high fat diet feeding-induced insulin resistance rats; To discuss acupuncture mechanisms to improve the insulin sensitivity. Methods Thirty-two Wistar rats at the age of eight weeks were randomly divided into normal group, model group, electro-acupuncture group, and sham electro-acupuncture group. The normal group was given basic diet, and model group, electro-acupuncture group and sham electro-acupuncture group were given high fat diet for 8 weeks, after which, the model of insulin resistance was successfully obtained. Electro-acupuncture group was given acupuncture therapy with “Guanyuan (GV4)”, “Zhognwan (GV12)”, “Zusanli (ST36)”, and “Fenglong (ST40)” for 8 weeks. Sham electro-acupuncture group was given stimulation at non-acupuncture points which were not on the meridians and near the acupoints but not at the area of the acupoints. In addition, food intake and body weight of each group were recorded, blood glucose was tested, TG content of quadriceps femoris was measured by enzymatic method after the treatment. Results The weight of the rats and TG levels of quadriceps femoris in electro-acupuncture group and sham electro-acupuncture group were significantly lower than those in model group, with significant statistical difference (P<0.05), especially in electro-acupuncture group. Conclusion Acupuncture therapy could improve insulin sensitivity to prevent and its related diseases.

A total of 189 stool samples from swine with diarrhea, collected in various porcine farms in the central region of China were tested for porcine enteric caliciviruses (PEC) member porcine sapoviruses (SaV) by reverse transcription polymerase chain reaction (RT-PCR) amplification using primers designed to detect porcine SaV. Selected amplicons were sequenced to establish phylogenetic relationships with reference strains. Porcine SaV were detected in 12.70% (24/189) of the samples. Phylogenetic studies based on partial RNA polymerase gene sequences indicated that the field strains of viruses isolated in China were closely related (75.6 88.3% identity) to the porcine SaV Cowden reference strain. These results provide evidence that caliciviruses of the genus sapovirus circulate in piglets in China, but further studies are needed to clarify their importance as cause of diarrhea. This is the first report of PEC in China.

OBJECTIVE:To establish a method for the concentration determination of voriconazole in human plasma.METHODS:Plasma samples were precipitated with acetonitrile.Using ketoconazole as internal standard,HPLC method was adopted.The determination was performed on Dionex U-3000 Dimonsil C18 column with mobile phase consisted of triethylamine-glacial acetic acid-water mixed solution (1 ∶ 1∶98,V/V/V,pH was about 4.0)-acetonitrile (40∶60,V/V) at the flow rate of 1.0 mL/min.The detection wavelength was set at 255 nm.The column temperature was 40 ℃,and sample size was 20 μL.RESULTS:The linear range of voriconazole was 0.2-20.0 μg/mL.The limits of quantification was 0.2 μg/mL,and the minimum detection limit was 0.03 μg/mL.RSDs of inter-day and intra-day were lower than 10％.The method recoveries were 92.06％-106.26％ (RSD＜5％,n=5),and extraction recoveries were 75.62 ％-90.59 ％ (RSD ＜ 5 ％,n=5).The plasma concentration of voriconazole in 10 children ranged 0.22-4.90 μg/mL (n =10).CONCLUSIONS:The method is simple,rapid,specific and can be used for drug monitoring of voriconazole.

Objective To analyze and discuss the specific effect and mechanism of Guizhi Fuling pill on angiogenesis in rats with endometriosis. Methods A rat model of endometriosis was established in 40 rats in the same batch, they were randomly divided into 4 groups. The model control group did not give any drugs; the low dose of Guizhi Fuling pill group was given 0. 5 g Guizhi Fuling pill every day; the high dose of Guizhi Fuling pill group was given 3. 0 g cinnamon Fuling pill every day; gestation three ketone group was given three ketene 2 g every day. After 4 weeks of continuous administration, the rats were killed and the expression of VEGF and HIF-1 alpha in the rats was determined. The mechanism of Guizhi Fuling pill in the treatment of endometriosis was determined through the expression of VEGF and HIF-1α. Results Guizhi Fuling pill significantly inhibited the level of intravascular VEGF in rat endometriosis and inhibited the level of mRNA expression of HIF-1α. Conclusion Guizhi Fuling pill can inhibit the angiogenesis of endometriosis, and its mechanism is related to the inhibition of VEGF and HIF-1 alpha.