Adult ; Aged ; Chronic Disease ; Female ; Gram-Positive Cocci ; isolation & purification ; Humans ; Male ; Middle Aged ; Nasopharyngeal Neoplasms ; microbiology ; radiotherapy ; Radiotherapy ; adverse effects ; Sinusitis ; etiology ; microbiology ; Young Adult

Objective To investigate the influence of iodine excess on expression of TRAIl/TRAIL-sR1 in NOD and Balb/c mice and to study the effect of TRAIl/TRAIL-sR1 on the pathogenesis of experimental autoimmune thyroiditis(EAT). Methods Both Balb/c and NOD mice were divided randomly into control and iodine excess group by feeding with water containing no NaI or 0.05% Nal. The mice were sacrificed after 8 weeks. TRAIL and TRAIL-sR1 mRNA levels were detected by RT-PCR. The function, morphology and apoptosis of thyroids were also observed by ELISA and Tunnel stain. Results Treated by HI, enlarged follicles and flattened epithelium by accumulation of colloid were found in thyroids of both NOD and Balb/c mice. But significant lymphoid cell infiltration and local fibrosis were only found in thyroids of NOD HI group. The relative weight of thyroids of NOD mice in HI group[(104.8±14.5)mg/kg]was heavier than that of control group [(71.8±20.4)mg/kg]. The level of TT4 declined in HI group[(30.77±3.59)mmol/L]compared with control group[(36.43±2.66)mmol/L], meanwhile, the level of TSH was higher in HI group[(6.98±0.66)μg/L]than that in control group [(5.55±0.56)μg/L]. The difference being statistically significant(t=7.773,-9.526,-4.458, all P < 0.05). The relative weight of thyroids of Balb/c mice of HI group[(155.8±20.8)mg/kg]also heavier than that of control group [(105.1±22.0) mg/kg]. The level of TT4 droped in HI group [(19.75±3.32) mmoL/L]was higher than that in control group[(23.46±6.21)mmoL/L], the level of TSH in HI group[(4.14±1.71)μg/L]was higher than that in control group[(3.55±1.41)μg/L], the difference being statistically significant(t=7.554,-7.239,3.140, all P< 0.05). A great deal of apoptotie ceils observed in NOD (3.97±0.91) and Balb/c mice (1.05±0.45) by Tunnel stain were greater than control groups (0.21±0.15, 0.10±0.03), the difference being statistically significant in beth of the two species(t=-7.167,-17.772, both P < 0.05). The apoptosis index of thyroid follicular epithelium in NOD was obviously higher than Balb/c(t=-7.625, P<0.05). The level of TRAIL mRNA did not remarkably change in Balb/c between control group(0.000 59±0.000 39) and HI group(0.001 24±0.000 46, t=-1.940, P>0.05), but it increased apparently in NOD mice HI group(0.018 88±0.005 77) than that of control group(0.009 61± 0.00591, t=-2.71, P<0.05). The level of the expression of TRAIL-sR1 mRNA increased in HI groups of NOD (0.000 53±0.000 15) and Balb/c mice(0.000 42±0.000 09) than that in control groups of NOD(0.000 28± 0.000 05) and Balb/c mice (0.000 17±0.000 06) and the differences were statistically significant between the two species(t=3.050,3.990, all P<0.05). The differences of the expression of TRAIL and TRAIL-sR1 mRNA between the two species were significant(t=-3.37,-4.76, all P<0.05). Conclusions Iodine excess induces colloid goiter in beth species of mice and thyroiditis in NOD mice. The increase of TRAIL and TRAIL-sR1 influenced by iodine excess is one of the molecular bases of follicular epithelium apoptosis and inflammation in thyroids. Genetic factor is a key factor in the pathogenesis of thyroiditis.

OBJECTIVETo discuss the pathological characteristics of cervical spinal fracture complicating ankylosing spondylitis (AS) and the effect of combined anterior and posterior operation.

METHODSEighteen AS patients with traumatic cervical fracture-dislocation were treated operatively from January 2000 to January 2006. The symptom duration of AS was 14.5 years on average. Three cases had undergone osteotomy in lumbar spine. There were 4 cases of Grade A, 3 cases of Grade B, 9 cases of Grade C and 2 cases of Grade D according to Frankel's score. There were 15 cases of Grade III dislocation and 3 cases of Grade II. All patients underwent surgical procedures by combined anterior and posterior approach.

RESULTSThere were 4 anterior-posterior procedures, 8 anterior-posterior-anterior procedures and 6 posterior-anterior procedures. Seven patients had one stage operation and 11 cases underwent two stage surgeries. There was certain extent of neurological improvement in 14 incomplete paraplegic patients, but no improvement in 4 complete paraplegic patients. The follow-up period was 21.2 months on average and the time for bone fusion was 3.6 months. There were 4 complications during operation and a long-term complication in follow-up.

CONCLUSIONSThe study suggests that anterior combined with posterior approach makes the spine stable and relieves the pressure immediately. It is a reasonable surgical strategy for treatment of cervical spinal fracture-dislocation with AS.

Adult ; Cervical Vertebrae ; injuries ; Humans ; Joint Dislocations ; surgery ; Male ; Middle Aged ; Postoperative Complications ; etiology ; Retrospective Studies ; Spinal Fractures ; surgery ; Spondylitis, Ankylosing ; complications

Metabolomics is an omics subject which studies on the metabolic network of organism and the intrinsic metabolic change of entirety with the aim of clarifying the mechanism of medicinal effect and pathogenesis of disease, is similar to the whole concept theory of Chinese medicine. The mectabolomics technology helps to promote the modern process of traditional Chinese medicine. This article illustrated the application research on the concept of metabolomics, the syndrome of Chinese medicine, quality and components of traditional Chinese medicine, traditional Chinese medicine compounds and so on, explored the problems on the current research and the prospect meanwhile.

As a new method for simultaneous imaging of endogenous low molecular weight (＜ 1000 D) metabolites of complex biological samples through quantitative and qualitative analyses,metabolomics has been applied to biomarkers discovery,diagnosis,treatment,and prognostic judgement of diseases.Blood deficiency and blood stasis syndroms are common traditional Chinese medicine syndromes.Metabolomics can reflect the dynamic change regularity of metabolites of blood deficiency and blood stasis.The object of this article is to review the application of metabolomicsin blood deficiency and blood stasis.

Objective To study the difference effects of enriching blood function of different medicinal parts in Angelica on the hemolytic blood deficiency in rats.Methods Wistar rats were randomly divided into 6 groups:blank group,model group,positive control group,Angelica group,Angelica body group,Angelica tail group.The hemolytic blood deficiency rats was established by acetyl phenylhydrazine hydrochloride.Rats in normal and model groups were orally administered with saline,while rats in positive control group were orally treated with Lvjiaobuxue granule at the dose of 3.60 g · kg-1.Water decoction of Angelica,Angelica body and Angelica tail groups were orally at the dose of 10.8g · kg-1.All the drugs were administered to rats for consecutive 12 d.The contents of red blood cell (RBC),hematocrit (HCT),hemoglobin (HGB),platelet (PLT),mean corpuscular volume (MCV),mean corpuscular hemoglobin(MCH) and mean corpuscular hemoglobin concentration(MCHC) were detected by hematology analyzer.While liver index (LI),thymus index (TI),spleen index (SI),reticulocyte (RC),and marrow karyocyte were calculated.After collecting thighbone,marrow cell cycle was detected by flow cytometry method flow cytometry.Results With the treatment of Angelica and Angelica body water decoction,the whole blood contents of RBC,HGB,HCT,PLT,MCHC were (6.05 ±0.22),(6.12 ±0.12) 1012/L;(155.80 ±4.89)％,(154.40 ±5.19)％;(53.79±1.88),(53.27 ± 1.57) g · L-1;(68.21 ± 5.63),(67.53 ± 5.11) 1010/L;(289.70 ± 3.94),(289.91 ± 8.41)g · L-1,respectively in each group,which were obviously higher than that of model group (P ＜ 0.05,P ＜ 0.01).The contents of MCH,MCV in Angelica and Angelica body groups were (25.75 ± 0.44),(25.23 ± 1.09) pg;(88.88 ± 1.67),(87.01 ±2.72) fl,which were lower than that of model group(all P ＜0.05).After used by Angelica tail,the contents of RBC,HGB,HCT were (5.69 ± 0.40) 1012/L,(123.10 ± 3.70) ％,(51.41 ± 2.03) g · L-1,which were higher than that of model group (all P ＜ 0.05).Conclusion Angelica and Angelica body,in which has same enrichment blood effect has effect of enriching blood on the hemolytic blood deficiency rats,and Angelic tail has weaker effect of enriching blood.

Endophytes had plentiful biological diversity,and become an potential microorganism resource.The research on endophytes could have some significance for the protection of wild and endangered medicinal plants,expanding medicinal resources and the research and development of new drugs.The active metabolites of endophytes,interaction between endophytes and medicinal plants and influences of endophytes on genuine medicinal materials are reviewed.It will provide reference for further study on endophytes.

Objective To identify endophytes from Glycyrrhiza uralensis and study their anti-inflammatory effects in vivo and in vitro.Methods RAW264.7 cells in the logarithm growth period were made 1 × 106 cell · mL-1 to be single cell suspension.The blank group was added with 1640 culture medium,the model group was added with 1 mg mL-1 lipopolysaccharide (LPS)for culture,the control group was added with Glycyrrhiza water extracts(10,20,40 mg · L-1),and experimental group was added with 10,20,40 mg · L-1 endophyte fermentation products (JTYB006,JTYB012,JTYB029,JTYF023) for 2 h of treatment,then 1 nag · L-1 LPS was added for stimulation.The content of nitric oxide (NO) were measured by Griess,and interleukin-1β (IL-1β) and prostaglandin E2 (PGE2) were measured by ELISA.Kunming mice were randomly divided into 7 groups:model,control Ⅰ,control Ⅱ,experimental Ⅰ,experimental Ⅱ,experimental Ⅲ,experimental Ⅳ.Then xylene-induced mouse ear edema test and Glacial acetic acid-induced mouse abdominal capillary permeability increase test were conducted.Results Three strains of endophytic bacterium are Bacillus,belonging to 3 species,and 1 strain of endophytic fungi is Aspergillus ochraceus.The contents of NO,PGE2 and IL-1β were (89.53 ± 8.02) μmol · L-1,(420.85 ± 57.85) pg · mL-1,(35.24 ± 5.77) pg · mL-1 in model group.When the mass concentration was maintained at 40mg· L-1,the contents of NO were (65.60 ±4.31),(63.48 ±6.89),(62.30 ±5.92),(56.47 ±7.26) μmol · L-1,the contents of PGE2 were(254.07 ±43.62),(295.40 ±49.13),(280.65 ±52.91),(296.42 ±54.90) pg·mL-1,the contents of IL-1β were(21.34 ±5.36),(20.65 ±4.78),(16.67 ±3.98),(22.54 ±5.30)pg · mL-1 in 4 experimental groups.Comparison between model group and experimental groups showed that the differences were significant (P ＜0.05 or P ＜0.01).The ear swelling degree and the absorbance of abdominal Flushing fluid in model group were 17.99 ± 1.60,0.28 ±0.02.The ear swelling degree were 15.05 ± 2.38,13.68 ± 2.69,15.36 ±2.03,the absorbance of abdominal Flushing fluid were 0.24 ±0.04,0.18 ±0.05,0.21 ±0.05 in experimental Ⅰ,Ⅲ,Ⅳ groups.By comparison between model group and experimental groups,the differences were significant (P ＜0.05 or P ＜ 0.01).Conclusion The fermentations of endophytes in 3 strains (JTYB006,JTYB029 and JTYF023) have anti-inflammatory effects both in vitro and in vivo.

OBJECTIVETo explore the possibility of creating a rat new scar model by inserting gelatin sponge into rat excisional wounds.

METHODSTwo full-thickness wounds were created in each of total 49 SD rats. In the Experimental group (n = 19), a regular incisional wound (1 cm) was created on the left side, and an excisional wound of 1.0 cm x 0.2 cm was created on the right side with a gelatin sponge inserted. In control 1 group (n = 15), an excisional wound with sponge insertion was created on both sides of rats. In control 2 group (n = 15), two excisional wounds were created on both sides, and only one side wounds were inserted with a sponge. Animals were sacrificed at various time points for different examinations.

RESULTSThe wound/scar width increased 4 - 11 times in inserted wounds than in regular incisional wounds (P < 0.01), with an obvious delay of epithelialization. No difference in wound/scar width was found in both sides of wounds of control 1 group at various locations. In contrast to the linear scar of sponge-inserted wounds, contracted and irregular scar was found in non-inserted wounds of control 2 group.

CONCLUSIONSGelatin sponge insertion can create a thick linear scar in rat wounds, and thus provides a new model for scar research.

Animals ; Cicatrix ; pathology ; Dermatologic Surgical Procedures ; Disease Models, Animal ; Gelatin Sponge, Absorbable ; Male ; Rats ; Rats, Sprague-Dawley ; Skin ; pathology ; Suture Techniques ; Wound Healing