4.Simultaneous Electrochemical Detection of Uric Acid and Dopamine Based on Graphene Quantum Dot Modified Glassy Carbon Electrode
Jinghui JIANG ; Hongwei YU ; Ze ZHANG ; Dong CHANG
Journal of Modern Laboratory Medicine 2017;32(4):9-11
Objective To preparegraphene quantum dots (GQDs) and construct a novel biosensor for determination of dopamine (DA) and uric acid (UA).Methods The GQDs was prepared by carbonization method from citric acid as carbon sources.Differential pulse voltammetry was used by the modified electrodes to detect uric acid and dopamine,and the detection performance was evaluate.Collected three experimenters morning urine on July 29,2016.The proposed sensor was used for biological samples detection.Results The size of GQDs were between 3 to 5 nm,which was observed by transmission electron microscopy (TEM).The proposed sensor had good linear correlation of 50~600 μmol/L UA (r2 =0.996 6) and 2~ 240 μmol/L DA (r2 =0.992 5).In uric acid in urine samples' detection (n=3),RSD value was less than 3%.The standard addition recovery rates of UA and DA were in 95.7%~101.4% and 96%~102% respectively.Conclusion The biosensor based on GQDs for determination DA and UA had been constructed successfully.
5.Analysis on genetic diversity among wild Dipsacus asperoides by SCoT.
Da-xia CHEN ; Xue ZHANG ; Guang-lin CUI ; Yu WANG ; Long-yun LI ; Ze ZHANG
China Journal of Chinese Materia Medica 2015;40(10):1898-1903
To investigate the genetic diversity among wild Dipsacus asperoides in China, 66 germplasmic resources of D. asperoides were analyzed by Start Codon Targeted Polymorphism (SCoT) molecular markers. Genetic distance was calculated by TREECONW software and the systematic diagram of genetic relationship was clustered by UPGMA method. The results showed that the totals of 181 bands were detected using 20 primers , among which 109 were polymorphic bands. The average percentage of polymorphic bands was 60.13%. Genetic distance changed from 0.030 6 to 0.181 4. The clustering results showed that there was no significant correlation between the classification of the wild D. asperoides and their geographical origin. The relatively high genetic diversity of D. asperoides provides the basis for breeding new varieties.
China
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DNA Primers
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genetics
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DNA, Plant
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genetics
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Dipsacaceae
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chemistry
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classification
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genetics
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Genetic Variation
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Phylogeny
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Polymorphism, Genetic
6.Analysis on genetic diversity and genetic relationship of medicinal species in Dipsacus from China by SRAP.
Da-xia CHEN ; Xue ZHANG ; Yu WANG ; Long-yun LI ; Ze ZHANG
China Journal of Chinese Materia Medica 2015;40(13):2559-2564
The author detected the genetic diversity and genetic relationship within and among eight medicinal species of Dipsacus by the approach of sequence-related amplified polymorphism (SRAP). The associated genetic parameters were calculated by POPGENE 1.31. The Genetic distance was calculated by TREECONW and the systematic diagrams of genetic relationship were clustered by UPG-MA. The results showed that, using 26 primers, a total of 558 bands were produced, of which 539 were polymorphic loci. There was a high level of genetic diversity among species (PPB = 96.59%, Na = 1.9659, Na = 1.3375, H = 0.2143, I = 0.3423). However, genetic diversity was lower within species, the average of genetic parameters was PPB = 6.97%, Na = 1.0697, Na = 1.0311, H = 0.0187, I = 0.0291. The Nei's genetic differentiation coefficient was 0.9126, indicated that most of the genetic variation existed among species. By clustering analysis, different individuals gathered in the same group and the classified result of SRAP marker between traditional modal characters was almost same. The results confirmed that SRAP marker can be used as one of the effective methods to reveal the genetic diversity and relationship among medicinal species of Dipsacus.
China
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Dipsacaceae
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classification
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genetics
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Gene Amplification
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Genetic Variation
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Plants, Medicinal
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classification
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genetics
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Polymorphism, Genetic
7.Correlation of urinary iodine and thyroid function in elderly men
Zhi-peng, WANG ; Shuang, LIANG ; Ze-hui, FANG ; Hui, ZHANG ; Chun-yu, ZHAO ; Wei-bing, ZHANG
Chinese Journal of Endemiology 2012;31(2):216-218
ObjectiveTo study the correlation of urinary iodine and thyroid function in elderly men in Harbin,and to provide the basis for formulation of health measures for the elderly.MethodsSeventy five cases of clinically healthy elderly men were enrolled for check-up of urinary iodine,thyroid function and B-ultrasound in Geriatric Ward the Forth Affiliated Hospital of Harbin Medical University in 2010.The subjects of investigation were divided into iodine appropriate and iodine sufficient groups and thyroid function parameters and B-ultrasound results were compared.ResultsThe average age of the 75 cases of healthy elderly men was (79.07 ± 4.78) years old and the median of urinary iodine was 198.4 μg/L.There were 62.67% (47/75) elderly males whose iodine nutritional status was appropriate,but there were still some individuals(6.67%,5/75) in the iodine excess state.The level of TSH of the iodine appropriate group [(1.91 ± 0.82)mU/L] was lower than that iodine sufficient group [(4.98 ±0.60)mU/L,t =12.58,P < 0.05],while the level of FT3 of the iodine appropriate group[(4.71 ± 0.56)pmol/L]was higher than that iodine sufficient group[(3.31 ± 0.43)pmol/L,t =12.18,P < 0.05].But the difference of FT4between the two groups [(14.91 ± 3.12),(14.06 ± 2.79)pmol/L] was not statistically significant (t =1.40,P >0.05].The thyroid volume of iodine sufficient group[(20.9 ± 6.1 )cm3] was higher than that iodine appropriate group [(17.9 ± 5.6)cm3,t =2.11,P < 0.05].ConclusionsSufficient quantities of iodine intake may affect the thyroid of elderly people.Whether the quantity of iodine intake of the elderly population should be decreased or not need to be further studied.
8.Detection of congenital uterine malformation by using transvaginal three-dimensional ultrasound.
Li-Li, YU ; Xuan, ZHANG ; Ting, ZHANG ; Han-Rong, CHEN ; Ze-Hua, WANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(5):782-4
This study assessed the clinical application of transvaginal three-dimensional ultrasound (3D TVUS) in the diagnosis of congenital uterine malformation. A retrospective study was performed on 62 patients with congenital uterine malformation confirmed hysteroscopically and/or laparoscopically. The patients were subjected to transvaginal two-dimensional ultrasound (2D TVUS) and 3D TVUS. The accuracy rate was compared between the two methods. The accuracy rate of 3D TVUS was (98.38%, 61/62), higher than that of 2D TVUS (80.65%, 50/62). 3D TVUS coronal plane imaging could demonstrate the internal shape of the endometrial cavity and the external contour of the uterine fundus. It allowed accurate measurement on the coronary plane, and could three-dimensionally show the image of cervical tube, thereby providing information for the diagnosis of some complex uterine malformation. 3D TVUS imaging can obtain comprehensive information of the uterus malformation, and it is superior to 2D TVUS for the diagnosis of congenital uterine malformations, especially complex uterine anomaly.
9.Applications of adhibiting agent on tooth and bone sections.
Li-Hua HONG ; Ning MA ; Ze-Bing ZHANG ; Yu WANG ; Cheng-Ku LI
Chinese Journal of Pathology 2008;37(1):61-62
Animals
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Bone and Bones
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drug effects
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Dental Cements
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chemistry
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pharmacology
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Rats
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Tooth
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drug effects
10.Pharmacokinetics study on costunolide and dehydrocostuslactone after administration of traditional Chinese medicine Weichang'an pills.
Jing-ze ZHANG ; Lei WANG ; Zhao-xiang JIN ; Zhuo QU ; Yu-ling CHEN ; Wen-yuan GAO
China Journal of Chinese Materia Medica 2015;40(6):1173-1178
A HPLC-MS/MS multiple-reaction monitoring (MRM) quantitative analysis was made to establish a determination method for drug concentrations of costunolide (Co) and dehydrocostuslactone (De) in blood samples in the positive ion mode, with diazepam as the internal standard substance, in order to study the pharmacokinetic process of sesquiterpene lactones costunolide and dehydrocostuslactone after the oral administration of Weichang'an pills, and provide an theoretical basis for further studies on the substance basis for the anti-diarrhea effect of Weichang'an pills. In the blood samples, Co and De showed a good linearity within concentration ranges 0.700 0-769.7, 2.510-956.0 μg x L(-1), respectively. The results of precision, stability and recovery experiences proved the stability and reliability of the plasma concentration determination method. After the oral administration, the concentrations of Co and De in plasma increased with the increase in dose, with T(max) between 10.65-12.98 h, indicating a long time to reach peak plasma concentrations; C(max) of costunolide and dehydrocostuslactone ranged between 3.750-5.450,15.34-44.52 μg x L(-1), respectively. The in vivo adsorption of Co and De conformed to the one-compartment model, with a longer time to attain the peak plasma concentrations. These results provided an experimental basis for revealing the active substance basis and clinical medication of Weichang'an pills.
Administration, Oral
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Animals
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Drugs, Chinese Herbal
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administration & dosage
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chemistry
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pharmacokinetics
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Lactones
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administration & dosage
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blood
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pharmacokinetics
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Male
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Rats
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Rats, Wistar
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Sesquiterpenes
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administration & dosage
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blood
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pharmacokinetics
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Tablets
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administration & dosage
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chemistry
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pharmacokinetics