Objective :To observe therapeutic effect and safety of perindopril combined amlodipine (P+ A) and valsartan combined amlodipine (V+A) on hypertension .Methods :A total of 126 patients with hypertension treated in our hospital were enrolled.The patients were randomly and equally divided into V + A group and P+ A group ,both groups received corresponding treatment based on routine intervention for 12 weeks.Levels of systolic blood pressure (SBP) ,diastolic blood pressure (DBP) ,heart rate (HR) ,plasma nitric oxide (NO) ,endothelin (ET)-1 ,von Willebrand factor (vWF) ,serum cystatin C (CysC) and uric acid (SUA) before and after treatment ,therapeutic effect and incidence rate of adverse reac-tions were observed and compared between two groups .Results :Total effective rate of V+A group was significantly higher than that of P+A group (92.06% vs.79.37%) , P＝0.042. Compared with before treatment ,after 12-week treatment , there were significant reductions in levels of SBP ,DBP ,plasma ET-1 and vWF ,and significant rise in plasma NO level in two groups ;significant rise in serum CysC level in V+A group , P＝0.001 all.Compared with P+A group after 12-week treatment ,there were significant reductions in levels of DBP [(85.34 ± 6.27)mmHg vs.(80.25 ± 6.31)mmHg] ,SBP [(130.33 ± 10.18)mmHg vs.(125.61 ± 10.25)mmHg] ,plasma ET-1 [(63.48 ± 9.30)pg/ml vs.(54.32 ± 9.21) pg/ml] , vWF [(125.78 ± 13.37)% vs.(113.54 ± 13.26)% ] and serum CysC [(1.41 ± 0.31)mg/L vs.(0.89 ± 0.25)mg/L] ,and significant rise in plasma NO level [(75.48 ± 10.65) μmol/L vs.(82.94 ± 10.56)μmol/L] in V+A group ,P＜0.05 or ＜0.01. There was no significant difference in incidence rate of adverse reactions between two groups , P＝0.143. Conclu-sion :Valsartan and perindopril respectively combined amlodipine can effectively reduce blood pressure level in hypertensive patients ,but the former can more significantly improve vascular endothelial function with better therapeutic effect .

Objective: To explore the anti-lung cancer mechanism of Maimendong Tang and Qianjin Weijingtang (Jin Fang) by detecting the expression profiles of long noncoding RNA (lncRNA) and mRNA in mice tumor tissues of orthotopic Lewis lung cancer model. Method: C57BL/6 mice were randomly divided into normal group, model group and Jin Fang group (20 g·kg^-1·d^-1). After successful establishment of Lewis lung cancer model in situ in mice, Jin Fang was given orally the next day after treatment. Using gene chip technology, differential lncRNA and mRNA closely related with Jin Fang' s anti-lung cancer effect were detected, and cluster analysis was performed. The key lncRNA and mRNA were screened out by t-test and fold change of differential expression. Bioinformatic methods were used to predict target genes regulated by differential lncRNA, and functional and pathway analysis was performed. The histopathological technique was used to detect the differences in the tumor tissue of each group under light microscope. Result: lncRNA and mRNA chip hybridization results showed that Jin Fang regulated differential expressions of 887 lncRNA, in which 442 were up-regulated and 445 were down-regulated (P<0.05). There were 610 differential mRNA expressions, in which 376 were up-regulated and 234 were down-regulated(P<0.05). GO analysis showed that down-regulated or up-regulated target genes were mainly involved in biological processes (BP), such as cell metabolism regulation and signal pathway regulation. Molecular function (MF) analysis showed such functions DNA binding, protein binding, receptor binding and transcription factor activity in down-regulated or up-regulated target genes. Target genes were involved in multiple biological processes, cellular components and molecular functions. Signal pathway predictions indicated that Jin Fang can regulate the Janus kinase/signal transducer and activator of tran-ions(JAK/STAT) and other signaling pathways closely related to the development of lung cancer. The results of histopathological examination confirmed that Jin Fang could significantly improve the pathological changes of lung tissues in the mice compared with the model group. Conclusion: Jin Fang may exert its anti-lung cancer effect by regulating the expressions of multiple lncRNAs and mRNAs, and down-regulating related signaling pathways.

The present study was designed to investigate the effects of Laminaria japonica (Laminaria) on pharmacokinetics of glycyrrhetinic acid (GA) following oral administration of Liquorice extract in rats. Following oral administrations of single-dose and multi-dose Liquorice extract and Liquorice-Laminaria extract, respectively, plasma samples were obtained at various times and the concentrations of GA, liquiritigenin, and isoliquiritigenin were measured by LC-MS. The effects of Laminaria extract on pharmacokinetics of GA were also investigated, following single-dose and multidose of glycyrrhizic acid (GL). The effects of Laminaria extract on intestinal absorption of GA and GL were studied using the in situ single-pass intestinal perfusion model. The metabolism of GL to GA in the contents of small and large intestines was also studied. The results showed Liquorice-Laminaria extract markedly increased the plasma concentration of GA, accompanied by a shorter Tmax. Similar alteration was observed following multidose administration. However, pharmacokinetics of neither liquiritigenin nor isoliquiritigenin was affected by Laminaria. Similarly, Laminaria markedly increased concentration and decreased Tmax of GA following oral GL were observed. The data from the intestinal perfusion model showed that Laminaria markedly increased GL absorption in duodenum and jejunum, but did not affect the intestinal absorption of GA. It was found that Laminaria enhanced the metabolism of GL to GA in large intestine. In conclusion, Laminaria increased plasma exposures of GA following oral administration of liquorice or GL, which partly resulted from increased intestinal absorption of GL and metabolism of GL to GA in large intestine.
Administration, Oral ; Animals ; Drug Interactions ; Glycyrrhetinic Acid ; blood ; Glycyrrhiza ; chemistry ; Glycyrrhizic Acid ; blood ; pharmacokinetics ; Intestinal Absorption ; Intestinal Mucosa ; metabolism ; Laminaria ; Male ; Plant Extracts ; blood ; pharmacokinetics ; pharmacology ; Rats, Sprague-Dawley