1.Progress on research of salviae and salvianolic acid B in treating myocardial infarction with myocardial cell orientating differentiation of bone marrow mesenchymal stem cell.
Shi-long WANG ; Yuan LIN ; Ze-yao TANG
Chinese Journal of Integrated Traditional and Western Medicine 2010;30(12):1334-1337
Animals
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Benzofurans
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pharmacology
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therapeutic use
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Bone Marrow Cells
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cytology
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Cell Differentiation
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drug effects
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Drugs, Chinese Herbal
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pharmacology
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therapeutic use
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Humans
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Mesenchymal Stem Cell Transplantation
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methods
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Mesenchymal Stromal Cells
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cytology
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Myocardial Infarction
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drug therapy
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therapy
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Myocytes, Cardiac
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cytology
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Phytotherapy
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Salvia miltiorrhiza
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chemistry
3.Analysis on genetic diversity and genetic relationship of medicinal species in Dipsacus from China by SRAP.
Da-xia CHEN ; Xue ZHANG ; Yu WANG ; Long-yun LI ; Ze ZHANG
China Journal of Chinese Materia Medica 2015;40(13):2559-2564
The author detected the genetic diversity and genetic relationship within and among eight medicinal species of Dipsacus by the approach of sequence-related amplified polymorphism (SRAP). The associated genetic parameters were calculated by POPGENE 1.31. The Genetic distance was calculated by TREECONW and the systematic diagrams of genetic relationship were clustered by UPG-MA. The results showed that, using 26 primers, a total of 558 bands were produced, of which 539 were polymorphic loci. There was a high level of genetic diversity among species (PPB = 96.59%, Na = 1.9659, Na = 1.3375, H = 0.2143, I = 0.3423). However, genetic diversity was lower within species, the average of genetic parameters was PPB = 6.97%, Na = 1.0697, Na = 1.0311, H = 0.0187, I = 0.0291. The Nei's genetic differentiation coefficient was 0.9126, indicated that most of the genetic variation existed among species. By clustering analysis, different individuals gathered in the same group and the classified result of SRAP marker between traditional modal characters was almost same. The results confirmed that SRAP marker can be used as one of the effective methods to reveal the genetic diversity and relationship among medicinal species of Dipsacus.
China
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Dipsacaceae
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classification
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genetics
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Gene Amplification
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Genetic Variation
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Plants, Medicinal
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classification
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genetics
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Polymorphism, Genetic
4.Analysis on genetic diversity among wild Dipsacus asperoides by SCoT.
Da-xia CHEN ; Xue ZHANG ; Guang-lin CUI ; Yu WANG ; Long-yun LI ; Ze ZHANG
China Journal of Chinese Materia Medica 2015;40(10):1898-1903
To investigate the genetic diversity among wild Dipsacus asperoides in China, 66 germplasmic resources of D. asperoides were analyzed by Start Codon Targeted Polymorphism (SCoT) molecular markers. Genetic distance was calculated by TREECONW software and the systematic diagram of genetic relationship was clustered by UPGMA method. The results showed that the totals of 181 bands were detected using 20 primers , among which 109 were polymorphic bands. The average percentage of polymorphic bands was 60.13%. Genetic distance changed from 0.030 6 to 0.181 4. The clustering results showed that there was no significant correlation between the classification of the wild D. asperoides and their geographical origin. The relatively high genetic diversity of D. asperoides provides the basis for breeding new varieties.
China
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DNA Primers
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genetics
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DNA, Plant
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genetics
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Dipsacaceae
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chemistry
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classification
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genetics
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Genetic Variation
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Phylogeny
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Polymorphism, Genetic
5.Synthesis and evaluation for anti-HCoV-OC43 activity of novel aloperine derivatives with different core structures
Run-ze MENG ; Yue GONG ; Yu-long SHI ; Kun WANG ; Zong-gen PENG ; Dan-qing SONG
Acta Pharmaceutica Sinica 2024;58(2):404-412
In this study, we designed and synthesized 12 novel aloperine derivatives with different core structures. Among them, compound
6.Microbial degradation of aniline by bacterial consortium.
Jian-Long WANG ; Ze-Yu MAO ; Wei-Zhong WU
Biomedical and Environmental Sciences 2003;16(4):398-404
OBJECTIVETo investigate the characteristics of microbial degradation of aniline by a stable bacterial consortium.
METHODSThe bacterial consortium was isolated from activated sludge treating chemical wastewater using aniline as the sole source of carbon and nitrogen by enrichment and isolation technique. The biomass was measured as optical density (OD) at 510 nm using a spectrophotometer. Aniline concentrations were determined by spectrophotometer. The intermediates of aniline degradation were identified by GC/MS method.
RESULTSThe bacterial consortium could grow at a range of aniline concentrations between 50 and 500 mg/L. The optimal pH and temperature for aniline degradation were determined to be 7.0 and 30, respectively. The presence of NH4NO3 as an additional nitrogen source (100-500 mg/L) had no adverse effect on bacterial growth and aniline degradation. The presence of heavy metal ions, such as Co2+, Zn2+, Ni2+, Mn2+ and Cu2+ had an inhibitory effect on aniline degradation.
CONCLUSIONSThe isolated bacterial consortium can degrade aniline up to 500 mg/L effectively and tolerate some heavy metal ions that commonly exist in chemical wastewater. It has a potential to be applied in the practical treatment of aniline-containing wastewater.
Aniline Compounds ; metabolism ; Bacteria ; Biomass ; Bioreactors ; Carcinogens ; metabolism ; Chemical Industry ; Hydrogen-Ion Concentration ; Metals, Heavy ; analysis ; Waste Disposal, Fluid ; methods ; Water Pollutants ; metabolism
7.Self-made ultrasound/fluorescent bi-functional contrast agent for rabbit's normal lymph node imaging.
En-ze QU ; Zhi-fei DAI ; Shu-min WANG ; Xiao-long LIANG ; Heng-te KE ; Jin-rui WANG
Acta Academiae Medicinae Sinicae 2013;35(4):411-415
OBJECTIVETo prepare a lymph node-targeted ultrasound/fluorescence bi-functional imaging contrast agents, and observe its effectiveness both on contrast-enhanced ultrasound (CEUS) and vivo near infrared fluorescence (NIR) imaging through animal experiments.
METHODSThe chimeric lymph node-targeted ligand (phosphatidylserine) and near-infrared fluorescent substance were assembled to form bi-functional contrast microbubbles. The morphology and size distribution were detected by optical microscope and Malvern potential tests. Five normal New Zealand white rabbits were subcutaneously injected with the prepared contrast agent in bilateral footpads, and the imaging effectiveness of lymph nodes and lymphatic vessel were observed by CEUS and NIR technique. Then blue dye was subcutaneously injected at the same site, and the rabbits were sacrificed for lymph nodes pathological examination.
RESULTSLipid ultrasound microbubbles,with a mean size of 3-5 Μm in diameter, appeared to be uniform in distribution and regular in configuration. The images of inflow lymphatic vessel and relevant lymph node were quickly showed up after the subcutaneous injection by CEUS, which was identical to the result detected by NIR. Biopsy confirmed that all the blue-stained lymph nodes could be displayed by NIR.
CONCLUSIONSThe self-made bi-functional contrast agent has a good imaging ability in CEUS and NIR imaging. It may be a better agent as lymph node tracer.
Animals ; Contrast Media ; chemistry ; Fluoresceins ; chemistry ; Lymph Nodes ; anatomy & histology ; diagnostic imaging ; Lymphatic Metastasis ; pathology ; Male ; Rabbits ; Ultrasonography
8.HB-1, an acute myeloid leukemic cell line with the capability of infiltrating into the brain in CBA/N mice.
Hui-Jie JIANG ; Hu-Shan SUN ; Xu-Dong WANG ; Chang-Liu WANG ; Ze-Long LIU ; Hiroyuki GONDA ; Kenkichi SUGIMOTO
Acta Physiologica Sinica 2006;58(4):377-383
An acute myeloid leukemic HB-1 cell line was cloned and established from the spleen cells of irradiated CBA/N mice. Acute myeloma leukemia-like syndrome would be induced in normal CBA/N mice after intravenous injection of HB-1 cells, and the death of mouse happened within about two weeks. In general, leukemic cells transplanted into the mice would infiltrate into the hematopoietic organs, lungs, kidneys and liver. An interesting observation in our study was that HB-1 cells were present not only in the lung, kidney, and liver but also in the cerebrum and cerebellum. It was beyond our expectation that the leukemic cells could go through the blood-brain barrier in most circumstances. On the basis of the observation, we expect that HB-1 cells could be used as a very useful model to elucidate the mechanism of infiltrating the blood-brain barrier for certain type of cells.
Animals
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Blood-Brain Barrier
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physiopathology
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Brain
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pathology
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Cell Line, Tumor
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Central Nervous System Neoplasms
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Leukemia, Experimental
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pathology
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Leukemia, Myeloid, Acute
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pathology
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Mice
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Mice, Inbred CBA
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Neoplasm Invasiveness
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Neoplasm Transplantation
9.Clinical observation on hemiplegia early rehabilitation effects of different acupuncture programs.
Long-ming LEI ; Jun PANG ; Jia-xing CHEN ; De-hui PENG ; Ze-tao WANG
Chinese Acupuncture & Moxibustion 2005;25(4):233-236
OBJECTIVETo observe early rehabilitation of two different acupuncture programs for hemiplegia due to stroke.
METHODSSeventy cases of early hemiplegia due to stroke were randomly divided into a treatment group and a control group. The treatment group were treated by acupuncture at acupoints of both the healthy and affected sides, twice each day, respectively, and the control group by acupuncture at the affected side, once daily. Their therapeutic effects were evaluated by the brief Fugl-Meyer movement scale and the modified Barthel indexes.
RESULTSThe patients with over grade IV for FMA accounted for 68.6% and the good rate for ADL was 74.3% in the treatment group, and 31.4% and 42.8% in the control group, respectively, the therapeutic effect of the treatment group being better than that of the control group (P<0.01 or P<0.05).
CONCLUSIONThe acupuncture program in which acupoints at both the healthy and affected sides are applied alternately is more beneficial to recovery of motor function and activity daily living (ADL) of the patient with hemiplegia due to stroke.
Activities of Daily Living ; Acupuncture Points ; Acupuncture Therapy ; Hemiplegia ; therapy ; Humans ; Stroke ; therapy
10.Clinical observation on acupuncture at pain point combined with moxibustion at Zusanli (ST 36) for treatment of periarthritis of shoulder.
Xiang-Ling ZENG ; Long-Ming LEI ; Zheng LIN ; Ze-Tao WANG
Chinese Acupuncture & Moxibustion 2005;25(5):326-328
OBJECTIVETo find an effective therapy for periarthritis of shoulder.
METHODSSixty cases were divided into two groups, 30 cases in each group. They were treated by acupuncture at pain point combined with moxibustion at Zusanli (ST 36) and routine acupuncture, respectively. Pain of the shoulder and movement functions of shoulder joints were used as indexes of therapeutic effects and their therapeutic effects were compared.
RESULTSThe total effective rate was 96.7% in the treatment group and 90.0% in the routine group with no significant difference between the two groups (P > 0.05); the cured rate in the two groups were 46.7% and 20.0% respectively with a significant difference between the two groups (P < 0.05). There were statistically significant differences between the two groups in improvement of movement function of shoulder joints and alleviation of pain (both P < 0.01).
CONCLUSIONAcupuncture at pain points combined with moxibustion at Zusanli (ST 36) in increase of the cured rate and alleviation of pain, and improvement of movement function of shoulder joints is better than the routine acupuncture and moxibustion for treatment of periarthritis of shoulder.
Acupuncture Therapy ; Humans ; Moxibustion ; Pain ; Periarthritis ; therapy ; Shoulder