1.Solitary spleen metastasis of endometrial carcinoma: a case report.
Chinese Journal of Cancer 2010;29(1):30-31
Adenocarcinoma
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drug therapy
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pathology
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secondary
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surgery
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Chemotherapy, Adjuvant
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Endometrial Neoplasms
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drug therapy
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pathology
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surgery
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Female
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Humans
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Hysterectomy
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Middle Aged
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Splenectomy
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Splenic Neoplasms
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drug therapy
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pathology
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secondary
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surgery
2.Pulmonary hyalinizing granuloma: report of two cases.
Wen-ze WANG ; Hong-rui LIU ; Li-na GUO
Chinese Journal of Pathology 2006;35(8):505-507
Adult
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Female
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Granuloma, Respiratory Tract
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pathology
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Humans
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Lung, Hyperlucent
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pathology
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Male
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Middle Aged
3. Effect of ginsenoside Rh2(S) on apoptosis of human leukemia cell by inhibiting HDAC6
Chinese Traditional and Herbal Drugs 2018;49(24):5876-5881
Objective To explore the specific mechanism of ginsenoside Rh2(S) inducing the apoptosis of leukemia cells. Methods The effect of Rh2(S) on proliferation of leukemia cells K562 and KG1a was measured by cell counting kit-8 assay (CCK-8 assay). The growth states of cells were observed under the inverted phase microscope, and cell cycle distribution, and apoptosis were determined by flow cytometry (FCM). The expression levels of HDAC6, HSP90, Akt, GSK-3β, β-catenin, and cell cycle apoptosis related proteins were ascertained by Western blotting. Results The results of CCK-8 showed that Rh2(S) had the most obvious inhibitory effect on the proliferation of leukemia cells. Rh2(S) significantly induced apoptosis and led to cell cycle arrest at G0/G1 phase of leukemia cells by FCM. While the microscope observation showed that the number of cells was decreased and normal cell morphology changed. Rh2(S) decreased the expression of Bcl-2, Cyclin D1, HDAC6, HSP90, p-Akt, and β-catenin and increased the expression of Bax, Ac-α-tubulin, and GSK-3β by Western blotting. Conclusion Rh2(S) can effectively inhibit the proliferation and promote the apoptosis of K562 and KG1a cells, its specific mechanism may relate to inhibitting the expression of HDAC6, resulting in a decline in the expression of HSP90, so as to further inhibit the activity of Akt activation of GSK-3, and finally inhibit Wnt/β-catenin pathway.
4.Construction of prokaryotic expression vector, expression and purification of ginseng Cu/Zn superoxide dismutase.
Hong-Mei LIN ; Ze-Yu WANG ; Yue SHAO ; Xiao-Ye QIN ; Shi-Chao LIU ; Xin ZHANG ; Li-Min YANG
China Journal of Chinese Materia Medica 2013;38(23):4052-4055
The total RNA was extracted from ginseng leaves of Panax ginseng. The Cu/Zn-SOD gene was amplified via RT-PCR and the pET-28(a)-Cu/Zn-SOD expression vector was constructed. The pET-28 (a)-Cu/Zn-SOD recombinant plasmid was transformed into Escherichia coli BL21 (DE3) competent cells and was induced by IPTG in order to select optimal induction of expression conditions. The target protein was purified by the nickel ions (Ni ) affinity chromatography and the target protein enzyme activity was determinated by the xanthine oxidase method. The similarity of the Cu/Zn-SOD gene sequences and the Cu/Zn-SOD gene sequences of Korean ginseng in NCBI was 99. 00%. The target protein expression level was about 44.42%, and the molecular weight was 16.30 kDa after the pET-28(a)-Cu/Zn-SOD recombinants were induced by IPTG. The purified Cu/Zn-SOD protease activity reached 10,596.69 U x mg(-1). The P. ginseng pET-28(a)-Cu/Zn-SOD prokaryotic expression vector was built by the method of molecular biology, which provided the foundation for studying the Cu/Zn-SOD biology function.
Cloning, Molecular
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Escherichia coli
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genetics
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Gene Expression
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Genetic Engineering
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methods
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Genetic Vectors
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genetics
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Panax
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enzymology
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genetics
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Sequence Analysis
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Superoxide Dismutase
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genetics
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isolation & purification
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metabolism
5.Protective effect of acupuncture on heart in mice with hyperlipemia and its mechanism.
Hong-Bo SHEN ; Li ZHANG ; Jia GUO ; Xiao-Lan JI ; Bo PENG ; Fu-Yun LI ; Cheng LIU ; Ze-jun HUO
Chinese Acupuncture & Moxibustion 2014;34(4):373-378
OBJECTIVETo observe the inhibiting effect of acupuncture on blood lipid, myocardial hypertrophy and fibrosis in mice with hyperlipemia, and explore its possible action mechanism.
METHODSTen inbred mice (C57) were applied. Forty ApoE(-/-) mice who removed gene of apolipoprotein E were randomly divided into a control group, a non-acupoint group, an acupoint group and a medication group. The points 0. 5 cm and 1 cm next to the end of mice tail were respectively punctured in the non-acupoint group; "Neiguan" (PC 6) and "Fenglong" (ST 40) were punctured in the acupoint group; intragastric administration of simvastatin was applied in the medication group. After 8 weeks of treatment, the changes of total cholesterol (TC) and ratio of heart to body mass in each group were measured; changes of cardiac muscle fiber and ventricular wall thickness were observed; enzyme linked immunosorbent assay (ELISA) was used to test the level of angiotensin II (Ang I ) in plasma, and western blotting method was used to test protein content of angiotensin II type 1 receptor (AT1R) and endothelin-1 type A receptor (ETAR) in the heart.
RESULTSAfter 8 weeks of intervention, compared with the control group, rising range of blood lipid was obviously decreased (P<0.01) in the acupoint group and medication group, ratio of P<0.01), myocardial heart to body mass was decreased (P<0.05), thickness of ventricular wall was reduced (P fibrosis was relieved, levels of Ang II and ET-1 in plasma were decreased (P<0. 05), content of NO was increased (P<0. 05), and protein content of AT1R and ETAR was decreased in the heart (P<0. 05).
CONCLUSION40) could inhibit the rising of blood lipid in ApoE(-/-) mice, lower the levels of Ang II and ET-1 in peripheral blood, increase the content of NO and inhibit the expression of AT1R and ETAR in heart tissue, which could relieve myocardial hypertrophy and fibrosis to play a protective role on heart.
Acupuncture Points ; Acupuncture Therapy ; Angiotensin II ; metabolism ; Animals ; Blood Pressure ; Disease Models, Animal ; Heart ; physiopathology ; Heart Diseases ; etiology ; metabolism ; physiopathology ; prevention & control ; Humans ; Hyperlipidemias ; complications ; physiopathology ; therapy ; Male ; Mice ; Mice, Inbred C57BL ; Myocardium ; metabolism
6.Research progresses on NLRP3 inflammasomes-induced anti-tumor immunity
Cui-cui SUN ; Jing-wen DONG ; Ze-an KUANG ; Ming-xiao YIN ; Xiao-jia LIU ; Hong-bin DENG
Acta Pharmaceutica Sinica 2022;57(9):2612-2621
More and more studies have shown that NOD-like receptor protein 3 (NLRP3) inflammasome has become the regulatory factor of inflammatory response and protective immunity, and the assembly and activation of NLRP3 inflammasomes are closely related to the anti-tumor immunity effect. Depending on the cell type and stimuli, activation of the NLRP3 inflammasome can induce immune cells to become polarized, hyperactive, or pyroptotic, releasing interleukin (IL)-1
7.Clinical observation on the influence of psychological intervention to the mental state after chemotherapy of breast cancer patients in the rural area
Chinese Journal of Modern Nursing 2008;14(27):2861-2863
Objective To assay the clinical observation about the influence of psychological intervention to the mental state after chemotherapy of breast cancer patients in the rural area. Methods A total of 120 breast cancer patients in the rural area were randomly divided into the treatment group (n=60) and the control group (n=60). The treatment group was treated with the active psychological interventions, and the control group was treated with the routine nursing and health education. The mental health states of the two groups were measured by SCL-90 self measuring scale and self rating depression/anxiety scale designed by Zung, then the changes of mental health states before and after the intervention were compared. Results The score of SCL-90 self-measuring scale of the treatment group was (1.54±0.33), which was significantly lower than (1.82±0.43) in the control group after the psychological interventions. The grades of depression and anxiety of the treatment group were significantly lower than those of the control group. The differences between the two groups had statistical meanings (P< 0.05). Conclusions The nuring on basis of traditional therapy, combined with systemic psychological intervention, can improve the mental state of breast cancer patients with chemotherapy.
8.Study of oral care on improving cough reflex sensitivity of recurrent pneumonia patients
Ze-Zheng HAN ; Hui LIU ; Yan-Hong FANG ; Ai-Hong CHENG ; Huan-Huan ZHAO ; Hong-Mei YU ; Chang-Rong WU
Chinese Journal of Modern Nursing 2009;15(7):624-625
Objective To explore the effects of oral care on improving cough reflex sensitivity and prolonging time span of recurrent pneumonia. Methods Oral care was conducted among 19 pateints with recurrent pneumonia. Using capsaicin-induced cough reflex method, cough reflex thresholds were measured in 19 patients with recurrent pneumonia before the study began, at the 20th day and the twelfth month after the study began. The recorrent time spans were recorded before and after the oral care at the same time. Results Cough reflex threshold was (1.54±0.06) mmol at the 20th day after the oral care began, which was significantly lower than (2.13±0.05) mmol before the the oral care began (P<0.05); There was no significant difference between the cough reflex thresholds at the 20th day and the twelfth month (1.61±0.08) mmol (P>0.05). Pneumonia recurrent time span (210±54)days was significandy longer after performing the oral care than that (143±71)days of before performing the oral care (P<0.05). Conclusions Oral care may improve the cough reflex sensitivity among pateints with recurrent pneumonia. It can prolong the time span of recurrent pneumonia.
9.Construction of Brucella Unmarked Deletion Mutant by Using Conventional Cloning Vector as Suicide Plasmid
Yu-Fei WANG ; Ze-Liang CHEN ; Hong-Qing ZHAO ; Xi-Tong YUAN ; Liu-Yu HUANG ; Ling ZHANG ; Jing-Mei LIU ; Hong-Bin SONG ;
Microbiology 1992;0(04):-
Construction of mutant strain is an essential method in pathogenesis researches. The conventional method for Brucella unmarked deletion mutant construction is based on suicide plasmid, but the efficiency is very low. In the present study, we first optimized the electroporation parameters, and then, the cloning plasmid pEX18Gm containing sacB was successfully used to construct unmarked deletion mutant of the type IV secretion system. This indicated that by using conventional cloning plasmid as suicide plasmid in Brucella, unmarked deletion mutants can be constructed with high efficiency.
10.Study of Helicobacterpylori on p38MAPK signal transduction pathway activation in gastric epithelial cancer cells line MKN45
Qi LI ; Zhong-Ze FAN ; Jue SUN ; Ning-Ning LIU ; Li-Hong ZHOU ; Qiong WU ; Yan WANG ; Hua SUI ; Hong GAO ;
China Oncology 2006;0(11):-
Background and purpose:Cyclooxygenase-2 (Cyclooxygenase-2,COX-2) is an important rate-limiting enzyme that is responsible for transformation of arachidonic acid into prostaglandins(PGs).Although Helicobacter pylori(Hp) infection-induced gastric over-expression of COX-2 (COX-2) is an important factor of gastric cancer,the mechanism of COX-2 expression in gastric mucosa cells infected with Hp is still not clear.Our study was to reveal the effect of Hp on expression of COX-2(cyclooxygenase-2),the impact of p38MAPK signaling pathway in human gastric epithelial cancer cells line MKN45,and to investigate the potential mechanisms of expression of COX-2. Methods:The expression of COX-2 mRNA infection by standard Hp NCTC11637 in human gastric epithelial cancer cells line MKN45 was evaluated by real-time fluorogenic quantitative polymerase chain reaction (RFQ-PCR).The effect of infection by Hp on COX-2 expression,activation of p38MAPK and its downstream of the ATF-2 was assayed by Western blot.Results:The expression of COX-2 mRNA in MKN45 cells infected by Hp compared with control group,COX-2 mRNA had 3 fold,7.2 fold,5.1 fold,4.3 fold up-regulation after 3 hrs,6 hrs,9 hrs,12 hrs,respectively. COX-2 mRNA expression in each time group was significantly higher than that in the control group(P