Objective To study the characteristics of two operation methods and to choose a better one for repairing the sunken upper eyelid deformity by comparing with the traditional operation methods. Methods Two kinds of operation method were used, in which the brow fat pad flap and free fascia fat tissue graft were transfered for repairing the sunken upper eyelid deformity. Results Four cases of sunken upper eyelid were repaired successfully using the transferring of brow fat pad flap. The operative results were satisfactory with less complications. The fat did not absorb after a year follow-up. Other 5 cases of sunken upper eyelid were repaired successfully using free fascia fat tissue graft. The fat absorbed a little after a year follow-up and the shape of the upper eyelid was satisfactory. Conclusion The inversion of brow fat pad flap is a new method to repair the sunken upper eyelid deformity and has the following advantages: ⑴the fat tissue transferred to the recipient site has good blood supply with no absorption, and the long term result is permanent; ⑵the construction of brow fat pad is similar to the orbital septum fat, and the result is better and more natural; ⑶the donor site is adjacent to the recipient site, and the brow fat pad can be transferred easily, and ⑷there is no apparent postoperative scar in the donor site. The fascia-fat composite is expected to have a better survival rate than free fat alone and to be lighter than a dermis-fat, and is much similar to the anatomical structure of the repair site. Therefore, the method of fascia fat graft is one of good methods to be selected to repair the sunken upper eyelid.

Objective To increase the quality of blood transfusion medical record and strengthen the management of clinical blood transfusion by establishing a management system for clinical blood transfusion.Methods The management system of clinical blood transfusion was developed by using Sybase PowerBuilder 10.5 program and Oracle 8/8i database,through the function module's development of blood application and evaluation by using C/S structure.Results The management system of clinical blood transfusion realized the exchange of the internal data information with the blood information management system and LIS database,and implemented online audit of transfusion application and evaluation,which improved the work efficiency and reduced the human error.Conclusion The management system of clinical blood transfusion can improve the quality of blood transfusion medical record and realize real-time regulation of clinical blood transfusion to ensure the safety of transfusion.

Objective: To study the effects of ginger oleoresins(GO) on blood lipids in hyperlipidemic mice and the platelet aggregation in rats.Methods: The rat platelet aggregation was induced by ADP and AA in vitro, and the inhibition by GO was tested by light transmission. The animal model of hyperlipidemia was establised by feeding Kunmimg mice with high lipid feed stuff. The effects of GO on serum lipid and hepatic MAD in hyperlipidemic mice was investigated. Results: GO could effectively inhibit rat platelet aggregation induced by ADP and AA in vitro, which were 58.9%,97.5% respectively, and in dose dependant manner. Besides, GO could also significantly decrease serum TC, LDL, AS index and hepatic MDA in hyperlipidemic mice, which were 16.45%, 60.45%,37.30% and 54.27% respectively, and showed no side-effect on liver. Conclusion: Long-term consumption of GO can effectively modulate blood lipids, prevent thrombosis and AS.

Objective Utilization of proteome method,by observing differences on protein expression of hypothalamus in the hyperthyroidism rats with hyperactivity of liver-yang and normal rats,to search the differential protein.Methods Models of animal were prepared by ip L-T4 and Fuzi Decoction.Both the quantitative and qualitative changes of the protein expression were compared between model group and treated group by protein technique.Results Proteins were mainly displayed at range of pI 3～8,Mr(14.4～75)kD.18 protein increased and 24 protein decreased in model group compared with treated group, included Thioredoxin,Ubiquitin carboxyl-terminal hydrolase isozyme L1,Platelet-activating factor acetylhydrolase IB gamma subunit,glutamate dehydrogenase,dihydropyrimidinase-related protein 2, NSFL1 cofactor p47,Tubulin beta 5.Conc(?)sion There were 18 spots high expressed and 24 spots low expressed in the hyperthyroidism with hyperactivity of liver-yang,that maybe related with the develepment of the hyperthyroidism with hyperactivity of liver-yang.

Female ; Heart Septal Defects, Atrial ; complications ; Humans ; Levocardia ; complications ; Situs Inversus ; complications ; Young Adult

Objective To observe the renal lesion caused by aristolochia manshuriensis kom(AMK) through 2 infants who had used AMK before hospitalization.Method Retrospecting the 2 cases of infants caused by AMK from 2002 to 2003,and evaluating their pathogenesis,treatment,and prognosis.Result Two infants both presented with symptoms of acute renal failure(ARF),and poor outcome.Conclusions Renal lesion in infant caused by AMK is serious.Some medcines,such as glucocorticosteroid,may be useful for its treatment and prognosis.

Objective To explore the pathogenesis and prognosis of acute renal insufficiency in children.Method The pathogensis,clinical manifestation,treatment and prognosis retrospected and discussed by analysis of the clinical features of 34 children with acute renal insufficiency hospitalized in 2002-2005.Results Of 34 children,there were 15 females and 19 males,the age range from 16 days to 15.5 years old.Among pathogenesis of acute renal insufficiency,primary glomerular diseases occupied 35.3% and drug-induced acute renal insufficiency occupied about 29.4%.The mortality of drug-induced acute renal insufficiency was 20% of and about 30%(deve-)loped chronic renal insufficiency was 30%.Conclusions Primary glomerular diseases rank the dominant causes of acute renal insufficiency,while drug-induced acute renal insufficiency has poor prognosis.So it is important to treat primary glomerular diseases in early stage and emphasize the side effect of drugs to kidney.

Objective:Establish mass-scale purification technology of cell-derived influenza virus. Methods: A microcarrier-based process was used to produce human influenza virus in serum free-adapted Vero cells. The virus was purified in a sequence of downstream processing steps including inactivation, clarification, anion exchange chromatography, affinity chromatography and size-exclusion chromatography. Results: The recovery of HA reached 102%. 95.3% total protein, including 99.77% host cell protein, and 99.99% host cell DNA were removed during downstream processing. Conclusion: Providing a high effective purification technology for cell-derived influenza virus.

A series of novel sorafenib analogues were designed and synthesized. The cytotoxic activities of these compounds were tested in four tumor cell lines. Some of the compounds showed potent antiproliferative activity against the tested cell lines with IC50 = 4-20 micromol x L(-1). Some compounds demonstrated competitive antiproliferative activities to sorafenib against tested cancer cell lines. Among them, compound 7c demonstrated significant inhibitory activities on ACHN, HCT116 and MDA-MB-231 cell lines with IC50 values of 9.01, 4.97, 6.61 micromol x L(-1), respectively.
Antineoplastic Agents ; chemical synthesis ; chemistry ; pharmacology ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Humans ; Inhibitory Concentration 50 ; Molecular Structure ; Niacinamide ; analogs & derivatives ; chemical synthesis ; chemistry ; pharmacology ; Phenylurea Compounds ; chemical synthesis ; chemistry ; pharmacology ; Structure-Activity Relationship

Agmatine ; administration & dosage ; pharmacology ; Analgesia ; methods ; Animals ; Injections, Spinal ; Male ; Morphine ; pharmacology ; Rats ; Rats, Sprague-Dawley ; Spinal Cord ; drug effects