Objective To study the epidemiological features of prevalence and distribution of diabetes mellitus (DM) and the injury glucose tolerance (IGT) in elder people in Beijing. Methods Cross over sectional epidemiological survey by random stratified sampling method in elder population was applied and the middle aged people was used as controls. The prevalent status of diabetes and IGT in the population more than 60 years old in urban area was compared with that in rural areas in Beijing in 1997. Results The aggregate age adjusted standardization prevalence of diabetes and IGT were 15 98% and 15 89% , respectively in old age population in Beijing in 1997. Diabetes prevalence in 60 69 years group (only comparable data) increased about 3 2 times from 4 27% in 1981 to 13 73% in 1997. Although it was no difference in IGT prevalence between urban and rural elders, there existed significant difference for diabetes standardized rates between them(17 74% vs 8 83%). The characteristics that diabetes occurrence increased with aging has been confirmed by the correlation of diabetes prevalence to the aging in both middle age( r =1 00, P

Adult ; Craniotomy ; methods ; Humans ; Male ; Nasal Cavity ; pathology ; Paranasal Sinuses ; pathology ; Skull Base Neoplasms ; surgery ; Teratoma ; surgery

Accidents, Occupational ; Acute Disease ; Humans ; Hydrogen Sulfide ; poisoning

Objective To increase the quality of blood transfusion medical record and strengthen the management of clinical blood transfusion by establishing a management system for clinical blood transfusion.Methods The management system of clinical blood transfusion was developed by using Sybase PowerBuilder 10.5 program and Oracle 8/8i database,through the function module's development of blood application and evaluation by using C/S structure.Results The management system of clinical blood transfusion realized the exchange of the internal data information with the blood information management system and LIS database,and implemented online audit of transfusion application and evaluation,which improved the work efficiency and reduced the human error.Conclusion The management system of clinical blood transfusion can improve the quality of blood transfusion medical record and realize real-time regulation of clinical blood transfusion to ensure the safety of transfusion.

Objective Assessment of genetic diversity and genetic structure of germplasm of wild Hedysari Radix. Methods Fifteen germplasms of wild Hedysari Radix. were collected from Wudu pupolations and Tanchang pupolations. ISSR (Intersimple sequence repeat) markers were used. ISSR data were analyzed with the program POPGEN 1.32. The UPGMA tree and PCoA analysis was constructed using Ntsys software. The AMOVA analysis used Arlequin 31 software and Population structure analysis used the structure 2.3.4 software. Results The results of the ISSR-PCR showed that 126 (72.83%) of the 173 ISSR locitested were polymorphic by the nine primers. The change of percentage of polymorphic loci (PPL) of wild germplasm populations were 49.71%-61.85%. The average of PPL was 55.78%. Two populations of species level of observed number of alleles (Na), effective number of alleles (Ne), Nei's gene diversity (H), and Shannon's information index was 1.728 3, 1.364 6, 0.224 2, and 0.334 5, respectively. Total gene diversity (Ht), gene diversity with provenances (Hs), the coefficient of gene differentiation (Gst), and estimate of gene flow from Gst (Nm) of cultivated and wild population were 0.223 0, 0.192 1, 0.138 9, and 3.099 4, respectively. The among-population component accounted for 16.36% of the total variation, while the within-population component accounted for 83.64%, and genetic variation occured mainly within the population. UPGMA analysis showed that 15 samples were clustered into two branches including Ⅰ and Ⅱ. PCoA and population structure analysis confirmed the partitioning results of the UPGMA clustering. Mantel correlation test showed that there existed middle-level correlations between the genetic differentiation and geographical distance. Conclusion Our studies showed that the genetic diversity of Hedysari Radix populations was at a higher level, the characteristics of genetic structure included genetic differentiation that occurs mainly within populations and perennials, which provides theoretical basis for protecting and utilizing germplasm of Hedysari Radix resources.

Objective: Taking the Hedysari Radix cultivated in different regions in Gansu province and its soil as the research objects, the correlation of contents between soil nutrients, including total N, total P, total K, organic matter, available P, available K, calycosin. and formononetin in Hedysari Radix were analyzed in this paper. Methods: The correlations of the contents between soil nutrients and active components were analyzed by the multiple comparisons, correlation analysis, and regression analysis. Results: The statistical results showed that there existed obviously positive correlations between calycosin and the contents of total N and total K of soil. Calycosin is positively correlated with the contents of organic matter and available P, while with available K and total P, it is negatively correlated. Meanwhile the contents of formononetin and total K existed significantly positive correlations. Formononetin was positively correlated with the contents of total N and available P, but it was negatively correlated with available K, organic matter, and total P. Conclusion: When the plant grows, it absorbs some ingredients of soil selectively. The accumulations of calycosin and formononetin are affected by the contents of total K, total N, and available P, which shows that the soil with higher total K, total N, and available P is conducive to the growth of Hedysari Radix.

Objective:To study the relation between the distribution ofapolipoprotein E(apoE)genotypes and cognitive impairment onset in long lived elderly in Bama area in Guangxi in china.Methods:A total of 112 long lived elderly aged 90 years old and over were collected and tested with MMSE to inspect their cognitive function,and they were classified into cognition impaired group and non-impaired group according to MMSE scores.We determined the AopE genotypes by way of PCR-RFLP technique,and compared the differences of AopE allele and genotype of the two groups.Result:The cognitive disfunction was found to be 14.29% in long lived elderly in Bama area.The ApoE ? 3/? 3 genotypes have highest frequency in long-lived elderly,next is ?2/3,and ?4/4 is lowest frequency.There were significant differences of ? 4 allele frequencies between cognition impaired group and non-impaired group(P

OBJECTIVETo assess the association between SIRT1 gene polymorphisms and the longevity phenomena in Yongfu region of Guangxi. In this case-control study, 500 individuals from Yongfu region of Guangxi were recruited. The subjects were divided into a longevity group (n=223, average age=93.17 U+00B1 3.08 yr) and a healthy control group (n=277, average age=46.92 U+00B1 17.12 yr). Polymerase chain reaction-high resolution melting curve (PCR-HRM) and DNA sequencing were used to determine the allelic and genotypic frequencies of rs3758391, rs3740051, rs2273773, rs4746720 and rs10997870 polymorphisms of SIRT1 gene in the two groups. The association between above polymorphisms and longevity was assessed.

RESULTSIn the longevity group, CT genotype of the rs4746720 locus was significantly more common than CC and TT genotypes (P=0.000, OR=2.098, 95%CI:1.412-4.117). However, no significant difference was found in the allelic and genotypic frequencies of rs3758391, rs3740051 and rs2273773 between the two groups.

CONCLUSIONThere is an association between rs4746720 of SIRT1 gene and longevity in Yongfu region of Guangxi.

Adult ; Aged ; Aged, 80 and over ; Alleles ; Asian Continental Ancestry Group ; genetics ; Base Sequence ; Case-Control Studies ; China ; Female ; Gene Frequency ; Gene Order ; Genetic Association Studies ; Genotype ; Humans ; Longevity ; genetics ; Male ; Middle Aged ; Polymorphism, Single Nucleotide ; Sirtuin 1 ; genetics ; Young Adult

Objective In order to cure some patients with the following characteristics which appear only when smiling: elevation and shortening of the upper lip, a transverse furrow below the co-lumella, and increased exposure of the maxillary gums, we reported a new surgical procedure to cor-rect this deformities. Methods By means of a transfixion incision extended laterally along the vestibu-lar or the upper gingivolabial sulcus incision, part of the depressor septi and orbicularis muscle were excised, and extensive separation were made to lower the upper lip. Results 27 cases of short upper lip were treated. Among the total, the transfixion incisions were made in 9 cases, and the upper gingi-volabial sulcus incisions were made in 18 cases. These procedures achieved lowering the upper lip, lighting or disappearing the transverse furrow, and covering the exaggerated exposure of the gums when patients smiled. At the same time the rhinoplasties were done in 6 cases. Through the above-mentioned we were able to correct the deformities on rhino-gingivolabial part only when the faces moved, achieved some kind of beauty for patients, and obtained satisfactory results. Conclusions Some patients have added defects .that are exported only when they begin to make facial movements. The cause of these characteristics is hypertrophy of the depressor septi nasi muscles. We describe the technique that is not only to correct the defects but also to enhance the patient's smile. It is worth to recommend for clinical application.

OBJECTIVE To explore a novel pH-sensitive fluorescent probe for in vivo tumor imaging. METHODS Zn5 were obtained in 140℃ after mixed with MeOH, water, Zn(NO3)2 · 6H2O, H4L and trimethylamine. The fluorescence spectra of Zn5 with the same concentration in different pH aqueous solutions were detected. And the stability of Zn5 was investigated by time dependent fluorescence emission spectra of Zn5 in BSA aqueous solution and 5.0% serum solution. Then, the cytotoxicity of Zn5 was detected by MTT assays. To clarify whether a similar fluorescence response occurs in biological organisms, HeLa cells were pretreated with probe Zn5 (0.5 μmol·L- 1) and fluorescence imaging were collected for targeting lysosomes in living cells because of lysosomes' acidic microenvironment. The A375 tumor-bearing mice were used to assess the imaging ability of Zn5 in vivo. Mouse tumor xenografts were established by injection of A375 cells with 2×106 cells per flank. Probe (1 μg·g-1) was administered to mice by injection. Images were obtained using IVIS Spectrum CT Imaging System. RESULTS There is a 11-fold intensity increasing as the pH values changing from 8 to 2. The almost unchanged emission intensities suggest Zn5 is stable in both BSA and serum. Zn5 has negligible cytotoxicity for HeLa, 293T and CHO-K1 cells. Zn5 can selectively display lysosomes in living cells. Both the 2D and 3D images in vivo distinguish the tumor from other tissues with good fluorescence contrast. CONCLUSION The high chemical stability, emission in the Vis/NIR range, pH sensitivity, a pKa located in the tumor pH range, and low toxicity make Zn5 is suitable for application as a pH- sensitive fluorescent probe for bio-imaging.