Recently, the clinical trials of combined immunotherapy for advanced hepatocellular were completed successfully (Imbrave150, Keynote524), which have significantly increased the objective response rate and prolonged median overall survival time. The success of combined immunotherapy provides great potential and reality for subsequent surgical intervention of advanced hepatocellular carcinoma. Nowadays, the hot topics of combined immunotherapy include the choices of combined immunotherapy, the perioperative period determination, tumor immune microenvironment, and effective biomarkers of immunotherapy selection, and so on. This paper intends to summarize the above-related issues.

In the clinical education of oral and maxillofacial plastic surgery,how to enhance students’study enthusiasm and interest,promote them to carry on the ponder,and sharpen their generalized analysis ability is a challenge for the OMPS(oral and maxillofacial plastic surgery) teachers.This article proposes a new teaching method:the stereo teaching method,and evaluate its effect.

Adolescent ; Adult ; Female ; Graft vs Host Disease ; diagnosis ; etiology ; prevention & control ; Hematopoietic Stem Cell Transplantation ; adverse effects ; Humans ; Male ; Middle Aged ; Risk Factors ; Young Adult

Objective To investigate if pretreatment with repeated electroacupuncture (EA) at Baihui acupoint could increase HSP 70 expression after cerebral ischemia-reperfusion injury in rats.Methods Twenty male SD rats weighing 280-320 g were randomly divided into 2 equal groups of ten : control group received no pretreatment and EA group received pretreatment with EA at Baihui acupoint 30 min a day for 5 days. The animals in both groups were anesthetized with isoflurane. Right common carotid artery (CCA) , external and internal carotid artery (EGA, 1C A) were exposed through a longitudinal incision in the neck. EGA was ligated and a 3-0 nylon monofilament (Ethicon) with rounded tip was inserted into ICA and threaded cephalad until resistance was felt. In this way middle-cerebral artery was occluded for 120 min in both groups. The nylon filament was then withdrawn to allow reperfusion. The animals were allowed to recover. At the end of 24 h reperfusion, the animals were anesthetized with intraperitoneal pentobarbital 40 mg?kg-1 and killed. Brain was immediately removed. Two pieces of brain tissue were obtained from right parietal lobe for microscopic examination (HE staining) and determination of expression of heat shock protein (HSP) 70 using immuno-histochemistry technique.Results In EA group the levels of HSP 70 expression were significantly increased (1.94?0.57) compared with those in control group (0.86?0.35) ( P

Objective:To obtain the optimal conditions for separating catalpol from leaves of Rehmannia by selecting appropriate macroporous adsorption resins.Methods:The detection indication was the content of catalpol, which was determined by HPLC method. Twelve different kinds of macroporous adsorption resins were studied on the static capacity of adsorption and desorption, and H103 resin was selected for the research of separation and purification.Results:The H103 resin had a good capacity for adsorption and desorption.The best process of purifying catalpol by H103 resin was 1mg/ml concentration, the adsorption rate of 1-2 BV/h,the flow rate of 1-3 BV/h, and 8 BV with 10% alcohol.Conclusion:The method is simple and available, which can simplify the production process and lower costs.

Objectives To investigate the expression of matrix metalloproteinase-9 (MMP-9) and its tissue inhibitor (TIMP-1) in cutaneous squamous cell carcinomas (SCC) and the relationship between the expression and invasive growth and metastasis of SCC. Methods Immunohistochemical method of streptavidin-peroxidase (SP) was used to detect the expression of MMP-9 and TIMP-1 proteins on the paraffin embedded sections of 65 patients with cutaneous SCC and 5 cases of normal epidermis. The immunohistochemical results were analyzed quantitatively using an image analysis system. Results MMP-9 and TIMP-1 proteins were diffusely expressed on the tumor nests and the mesenchymal cells around the nests, while in normal epidermis MMP-9 and TIMP-1 were weakly positive. The expression level of MMP-9 protein and the ratio of MMP-9/TIMP-1 were higher in aggressive SCC group than those SCC in situ group (t = 2.33 and 2.36, respectively, P

Objective To observe the change in IL-1β,IL-13mRNA expression in drowning rat lungs and serum,so as to investigate the significance of IL-1β and IL-13 mechanism in the development of drowning.Methods SD rats were randomly divided into control group,drowning group.Then using TaqMan probe method to determine the expression of IL-1β and IL-13 mRNA in Right lower lobe of lung tissue and the serum of right ventricle,which were extracted respectively from each group of rats.Results (1) The lung tissue morphological changes:Typical appearance signs and anatomy of drowning group meet ante-mortem drowning feature.(2) The expression of IL-1β,IL-13 in lung tissue:compared with the control group,the expression of IL-1β and IL-13 were slightly decreased,which has no statistical significance.(3) The expression of IL-1β and IL-13 in serum:compared with the control group,the expression of IL-1β and IL-13 were significant increased,both of which has statistical significance.Conclusion (1)The expression of IL-1β and IL-13 were decreased in lung tissue may be due to drowned rats present compensatory anti-inflammatory response syndrome which causes immune incompetent performance.(2) The expression of IL-1β and IL-13 were significant increased in serum may be relate to drown stress and drowning associated acute lung injury after traumatic stress.

Carcinoma, Non-Small-Cell Lung ; metabolism ; pathology ; Cell Membrane ; metabolism ; Cell Nucleus ; metabolism ; Cytoplasm ; metabolism ; Humans ; Lung Neoplasms ; metabolism ; pathology ; Signal Transduction ; TCF Transcription Factors ; metabolism ; Transcription Factor 7-Like 2 Protein ; Wnt Proteins ; physiology ; beta Catenin ; metabolism

Adult ; Dermatologic Surgical Procedures ; Female ; Humans ; Male ; Middle Aged ; Skin ; blood supply ; injuries ; Surgical Flaps ; Young Adult

Adolescent ; Amiodarone ; therapeutic use ; Anti-Arrhythmia Agents ; therapeutic use ; Child ; Child, Preschool ; Emergency Medical Services ; Female ; Humans ; Infant ; Male ; Prognosis ; Tachycardia, Ventricular ; complications ; pathology ; therapy ; Treatment Outcome