Objective To explore the distribution and drug resistance of pathogens isolated from patients with cholesteatoma otitis media so as to provide guidance for clinical use of antibiotics.Methods This survey analyzed the spectrum of organisms causing cholesteatoma otitis media and their sensitivities to commonly antimicrobial agents from Hebei province eye hospital in 2014.Results There was 86 positive speciments were cultured from 89 samples,the positive rate was 96.6％.A total of 90 strains of pathogens have been isolated,including 52 strains of gram-positive coccus (57.8％),35 strains of gram-negative bacilli (38.9％),3 strains of gram-positive bacilli (3.3 ％),and 0 strain of fungi.Staphylococcus epidermidis,Staphylococcus aureus and Staphylococcus chromogenes ranked the top three species of pathogens,accounting for 20.0％,16.7％,and 12.2％,respectively.The gram-positive cocci were susceptible to vancomycin,rifampicin and amikacin,and showed higher drug-resistancerate to penicillin,amoxicillin and azithromycin.When applied to gram-negative bacilli,the drugs with best resistance were penicillin and cefazolin,and the drugs with the highest sensitivity were levofloxacin and pipercillin/ sulbactam.Conclusion Staphylococcus is the predominant pathogens of cholesteatoma otitis media in hospital,and the bacteria have a high antibiotic resistance.Enhanced monitoring on pathogenic bacteria distribution and drug resistance analysis of cholesteatoma otitis media could benefitthe guide of clinical rational use of antimicrobial agents.

OBJECTIVE:To study the distribution law of Valsartan capsules in the ocular tissue of rabbit eyes after intragastri-cal administration. METHODS:3 rabbits(6 eyes)were intragastrically administrated Valsartan solution,7.5 mg every time,twice a day,for 6 d. Rabbits were executed after last administration,and the cornea,iris,aqueous humor,lens,vitreous and retina tis-sues were collected. HPLC was used to determine the content of valsartan in each tissue. RESULTS:Content of valsartan in retina, iris,cornea,aqueous humor,vitreous and lens were (4.274 ± 1.75),(2.233 ± 0.72),(0.871 ± 0.66),(0.713 ± 0.49),(0.177 ± 0.07),(0.083 ± 0.06)μg/g,respectively. CONCLUSIONS:After multiple administrations,valsartan distributes unevenly in each tissue of eyes,showing highest distribution content in retina,followed by iris,and lowest content in lens and vitreous.