Study on chemical coposition of essential oil from Zanthoxylum nitidum fruit collected at Me Linh district, Vinh Phuc province by GC/MS method. Results: the yield of essential oil from fruit was 0.35% by air-dry material, 27 constituents were identified, in which the main constituents were linalool L. (72.17%) and sabinen (11.24%)
chemistry ; Oils, Volatile ; Zanthoxylum

The determination of the contents and chemical components essential oil of Sen hoi (Zanthixylum rhetsa Roxl DC) collected in Mai Chau, Hoa Binh provine in Feb 2003 showed that the essential oil content reached 9,6% of dry product and 24 compouds was identified, among then the main compounds are benzen derivatives, 1- methoxy-4 (1- propenyl), benzaldehyd-4-methoxy, 1-butanon, 1- (4- hydroxy phemyl) and benzen-e,ethanol, alpha-ethyl-4-4 methoxy
Chemistry ; Oils, volatile ; Zanthoxylum

OBJECTIVETo study the chemical constituents from the stems of Zanthoxylum dissitum.

METHODColumn chromatography on macroporous resin and silica gel, and spectral analysis were used to isolate and elucidate the constituents.

RESULTSeven compounds were isolated and identified as 5,8-dimethoxyethane-3,4-epoxy-furanocoumarin (1), isoimpinellin (2), beta-sitosterol (3), lupeol (4), neohesperidin (5), beta-daucosterol (6), ursolic acid (7).

CONCLUSIONAll above the compounds were isolated from the plant for the first time and the compound 1 is a new compound.

Drugs, Chinese Herbal ; chemistry ; Plant Stems ; chemistry ; Zanthoxylum ; chemistry

OBJECTIVETo establish the characteristic fingerprint of Zanthoxylum nitidum by HPLC, and to provide a reference for the quality control of Z. nitidum in the market.

METHODThe established HPLC characteristic fingerprint of Z. nitidum, combined with similarity evaluation and system clustering analysis method, were applied to distinguish 25 batches of samples purchased from market preliminarily, to identify the authenticity and quality of Z. nitidum ingredients.

RESULTIn the 25 batches of samples purchased from market, only 8 batches were identified as genuine with good quality, 7 batches were identified as defective, 7 batches were identified as common counterfeit Toddalia asiatica, and 3 batches were identified as counterfeit.

CONCLUSIONThis method is accurate, convenient and reliable. It can be used for identification and quality control of Z. nitidum ingredients.

Chromatography ; Drugs, Chinese Herbal ; chemistry ; standards ; Quality Control ; Zanthoxylum ; chemistry

Zanthoxylum belongs to the Rutaceae family, and there are 81 Zanthoxylum species and 36 varieties in China. Most of the Zanthoxylum plants are used as culinary spice. In recent years, scholars in China and abroad have carried out in-depth research on Zanthoxylum plants, and found that the peculiar numbing sensation of Zanthoxylum plants originates from amides. It is also determined that amides are an important material basis for exerting pharmacological effects, especially in anti-inflammatory analgesia, anesthesia and other aspects. In this paper, 123 amides in 26 Zanthoxylum plants and their pharmacological activity that have been reported were summarized, which provided scientific reference for the clinical application of Zanthoxylum plants and the research and development of new drugs, and also facilitated the sustainable development and utilization of Zanthoxylum plant resources.
Zanthoxylum/chemistry* ; Amides/chemistry* ; Plant Extracts/pharmacology* ; China

OBJECTIVETo study the chemical constituents of the dried roots of Zanthoxylum dimorphophyllumr. spinifolium and to find out the active constituents of the plant.

METHODModern chromatography was used to purify chemical constituents, and their structures were identified by various spectral methods.

RESULTFour compounds were isolated and identified as isopimpinellin (I), xanthoxyletin (II), 6-(2', 3'-dihydroxy-3'-methyl-butyl)-7-hydroxy-2H-1-benzopyran-2-one (III), 6-(2'-O-beta-D-glucopyranosyloxy, 3'-dihydroxy-3'-methybutyl)-7-hydroxy-2H-1-benzopyran-2-one (IV).

CONCLUSIONAll of the above compounds were isolated from the above mentioned plant for the first time.

Coumarins ; chemistry ; isolation & purification ; Furocoumarins ; chemistry ; isolation & purification ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry ; Zanthoxylum ; chemistry

OBJECTIVETo study the chemical components from dried roots of Zanthoxylum dimorphophyllum var. spinifoliun.

METHODThe chemical components were isolated by low pressure column chromatography and their structures were identified by spectroscopic methods.

RESULTFive compounds were isolated and identified as 6-(2', 3'-dihydroxy-3'-methyl-butyl)-7-hydroxy-5-methoxy-2H-1-benzopyran-2-one (I), 6-(2',3'-dihydroxy-3'-methyl-butyl)-7-methoxy-8-(3'-methyl-but-2'-enyl)-2H-1-benzopyran-2-one (II),6-(2',3'-dihydroxy-3'-methyl-butyl)-7-hydroxy-2H-1-benzopyran-2-one (III), 6-(2', 3'-oxiranyl-3'-methyl-butyl)-7-methoxy-8- (3-methyl-but-2-enyl)-2H-1-benzopyran-2-one (IV), 7-methoxy-8-(3'-methyl-but-2'-enyl)-2H-1-benzopyran-2-one (V).

CONCLUSIONThese compounds were isolated from the plant for the first time.

Coumarins ; chemistry ; isolation & purification ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry ; Zanthoxylum ; chemistry

There are 250 species of Zanthoxylum (Rutaceae) in the world. This genus distributed in tropical and subtropical regions. Alkaloids are the major and representative ingredients in these plants including quinolines, isoquinolines, and amide alkaloids, with such biological activities as anti-tumor, anti-inflammatory, analgesic, anti-virus, anti-platelet aggregation, anti-bacteria and anti- oxidant. These species have been used for a long time to treat toothache, urinary and venereal diseases, lumbago and rheumatism. This review summarizes the chemical constituents and pharmacological activities from the Z. sppplants, in an effort to the systematic research and application of the alkaloids of this genus.
Alkaloids ; chemistry ; pharmacology ; Animals ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans ; Molecular Structure ; Zanthoxylum ; chemistry

OBJECTIVETo obtain the optimal conditions for separating the alkaloids from the extract of Radix of Zanthoxylum nitidum by selecting appropriate macroporous adsorption resins.

METHODEight types of macroporous adsorption were evaluated in separating efficiency with measuring the adsorption ratio and eluting ratio of Alkaloids as indexes.

RESULTThe XDA-5 macroporous adsorption resin had the best separating efficiency. After enrichment and purification with it, the product purity and yield of alkaloids were up to 33.25% and 90.15%, respectively.

CONCLUSIONThis method is simple, feasible and fit for industry production.

Adsorption ; Alkaloids ; chemistry ; isolation & purification ; Porosity ; Resins, Plant ; chemistry ; Zanthoxylum ; chemistry

"Huajiao" is dried ripe fruit peel of Zanthoxylum bungeanum or Z. schinifolium, is konwn as geoherbs, especially the "Dahongpao" cultivated in Hanyuan, Maoxian and Jiulong of Sichuan province. However, the genetic basis of Dao-di "Huajiao" is virtually unknown. The transcriptome of the fruit and leaf from Sichuan(Hanyuan, Jiulong, Lixian, Maoxian), Gansu(Wudu) province and Shaanxi(Fengxian) province was sequenced. Trinity de novo assembling resulted in a total of 177 616 unigenes. Through the KEGG, NR, SwissProt, Trembl, KOG/COG, GO, Pfam database comparision 106 644 annotated Unigene finally, 4 574 deferentially expressed genes were found in fruit between Sichuan and other provinces, including 3 740 up-regulated genes and 834 down-regulated genes. Among the up-regulated genes, 27 up-regulated genes were raleted to terpenoids, and 8 up-regulated genes were related to isoquinoline alkaloid bio-synthesis. Furthermore, it was also showed remarkable differences in groups which enrichment ratio of the diffe-rent expressed gene compared. The different expressed genes were annotated by the KEGG database into plant-pathogen interaction, plant hormone signal transduction and phenylpropanoid biosynthesis in fruit and leaf, but isoflavonoid bio-synthesis and betaine bio-synthesis were significantly different in fruit and leaf. The study laid a certain reference basis for comparison of quality and different expressed gene of Z. bungeanum from different groups.
China ; Fruit/chemistry* ; Gene Expression Profiling ; Plant Leaves/chemistry* ; Plants, Medicinal/chemistry* ; Secondary Metabolism ; Transcriptome ; Zanthoxylum/chemistry*