Objective To establish the reference intervals for thyroid-stimulating hormone(TSH) ,triiodothyronine(T3 ) ,thy-roxine(T4 ) ,free triiodothyronine(FT3 ) and free thyroxine(FT4 ) in healthy people aged between 18 and 60 years old in Haikou are-a .Methods According to the recommendations of clinical and laboratory standards institute(CLSI)C28-A3 document ,401 persons aged between 18 and 60 years old were recruited from Haikou(275 male ,126 female) .The makers were analyzed by gender .Estab-lished the reference intervals of TSH ,T3 ,T4 ,FT3 and FT4 in Haikou area .Results Reference intervals based on gender were estab-lished as TSH(0 .58-4 .60 mIU/L) ,T3 (1 .11-2 .31 nmol/L for male ,0 .97-2 .13 nmol/L for female) ,T4 (72 .24-152 .22 nmol/L ) ,FT3 (4 .17-6 .33 pmol/L for male ,3 .95-5 .47 pmol/L for female) ,FT4 (13 .16-21 .29 pmol/L for male ,12 .13-19 .61 pmol/L for female) .Conclusion This study has established the reference intervals of TSH ,T3 ,T4 ,FT3 and FT4 in healthy people aged between 18 and 60 years old in Haikou area .

Objective To investigate the failure of treatment with chloroquine in Yunnan in order to help formulate adequate antimalarial drug policy. Methods A World Health Organization 28-day in vivo test on therapeutic response for uncomplicated falciparum malaria in area with low or moderate transmission was adopted. Patients of age ≥ 6 months old were admitted without limitation in density of parasitaemia and body temperature. Clinical and parasitological observation was conducted for patients on day 0, 1, 2, 3, 4, 7, 14, 21, 28 and 35. Results Of 62 patients identified as malaria cases infected by Plasmodium falciparum only, Plasmodium vivax only or by both species, 52 cases infected by Plasmodium falciparum only were included in the study. The overall treatment failure rate was 40.7%, with early treatment failure (ETF) rate of 1.8% and late treatment failure rate (LTF) of 38.9%. Conclusion The treatment failure rate was much higher than the rate of 25% recommended by WHO. It is suggested that use of single chloroquine should be stopped in the treatment of falciparum malaria cases in such area. No relationship was found between the failure rate and the density of malaria parasites.

A salt-tolerant strain GYW capable of decolorating azo dye was isolated and identified as Halo-monas sp.by 16S rDNA.The result showed that the decolorizing salt-tolerant bacteria could survive above the 10% salt concentration and it could decolorize many dyes.The strain had a high decolorizing rate on acid red GR under the condition of pH 7.5,anaerobic 30?C and 10% NaCl.The ion of Cl- strong inhibited the decolorization of acid red GR,and the ion of SO42- affected little on the decolorization,and the lycine addi-tion with the optimal concentration of 200 mg/L could enhanced the decolorization rate under high NaCl concentration.

Objective To assess the value of magnetic resonance diffusion weighted imaging(DWI) and apparent diffusion coefficients(ADC) values of hepatic alveolar echinococcosis(HAE) in children.Methods 20 cases of children(≤14 years) with HAE were collected in this restrospective study.PNM staging was determined, the HAE peripheral area of DWI lesions with different P stages was observed, and the ADC value of the peripheral area was measured.The comparison of alveococcus lesions in different stages of DWI with continuous edge degree and ADC value difference was done to evaluate the growth activity.Results There were 5 cases of P1 lesions, 7 cases of P2 lesions, 2 cases of P3 lesions and 6 cases of P4 lesions.DWI features of peripheral area were as follows: High signal ring band between HAE lesion edge and adjacent normal hepatic parenchyma was observed.P1 lesions showed almost complete obviously high signal peripheral area, indicating the most active proliferation, P2 and P3 lesions of peripheral area were continuous and with high signal, and still had obvious growth activity.P4 lesions of peripheral area were not continuous, while the signal decreased, indicating the activity also decreased.The highest ADC value was detected in P1 lesions group of and the ADC value of P2 lesions group were lower than P1, and the ADC value of P4 lesions group were the lowest.P3 lesions samples were too small and thus no statistical analysis was done.Differences of ADC value between the three groups were statistically significant (P<0.05).Conclusion DWI image features could be used to assesse the growth activity of HAE in children with different stages to a certain extent.ADC values measurement provides important reference value for evaluating the growth activity at various stages of the lesions.

Objective To detect circumsporozoite protein (CSP) in anopheline vectors from south Yunnan and to evaluate ELISA in the detection. \ Methods\ Salivary glands of the anopheline mosquitoes were taken for finding sporozoites by microscopy and part of the glands was used for detecting CSP by ELISA. An. minimus was experimentally infected by blood from vivax malaria patient (with Plasmodium vivax) and examined for sporozoites and CSP. Eight species of anopheline mosquitoes were caught in the field and examined. Monoclonal antibodies to P.falciparum (Pf2A10) and P.vivax (Pv210, Pv247) were used in ELISA for detecting CSP. \ Results\ Sporozoites were found in the salivary glands of 27 out of 36 An. minimus experimentally infected (75\^0%), 29 were ELISA CSP positives (80\^6%), and 26 of the 27 mosquitoes showed Pv210 CSP positive. Among \{1 010\} parous anopheline mosquitoes from the field, 7 were found sporozoite positive (0\^69%), 8 were ELISA CSP positive (0\^79%), and 6 of the 7 mosquitoes showed CSP positive. Of \{4 675\} wild mosquitoes in 8 anopheline species with different ages, 11 were found CSP positive (0\^24%) including An.minimus, An.sinensis and An.maculatus with a positive rate of 0\^20%, 0\^24% and 0\^39% respectively.Among the 11 mosquitoes, 9 were Pv210 positive and 2 were Pf2A10 positive. Conclusion CSP detection by ELISA is a useful method to monitor the malaria transmission capacity of anopheline vectors.

Objective To identify a psoriasis susceptibility locus on chromosome4q in a Chinese Han population.Methods The genome search was performed using12microsatellite spanning chromosome4q in64Chinese Han families(372family members)comprising197affected and175unaffected individuals.GENEHUNTER was used for the parametric and non-parametric linkage analyses.Results(1)Non-paramet-ric linkage analyses:single-point analyses revealed that two adjacent loci on chromosome4q D4S413and D4S1597had evidence of linkage,with NPL-scores of2.04and2.23,and P values of0.021and0.014re-spectively.Multi-point analyses showed a peak NPL-score of3.44and the corresponding P value of0.00056at157.9cM where D4S413located.Moreover,NPL score of more than3was found with a range from155.1cM to172.3cM.(2)Parametric linkage analyses revealed a LOD score of3.70,a heterogeneity LOD score of4.35and a high proportion of linked families(?)of85%under the assumption of a dominant model with dis-ease-allele frequency of0.0062and penetrance of10%at D4S1597.Conclusion Chromosome4q contains genes involved in the susceptibility to psoriasis vulgaris in a Chinese Han population.

Objective To study the changes of hearing function and cochlear morphology on diffuse brain in-jury model in rat .Methods One hundred and fifty SD rats with normal hearing were randomly divided into five groups ,each group consisted of 30 SD rats ,including a control group and four experimental groups which endured diffuse brain injury(DBI) from one to four weeks .Diffuse brain injury model of rats were established ,then ABR , 40 Hz AERP and ASSR examination ,light microscopy ,electron microscopy were used to evaluate the change of hearing function and morphology .Results The difference of ABR ,40 Hz AERP and ASSR thresholds between the experimental and the normal control group were significant (P<0 .05) .The thresholds of ABR ,40HzAERP and AS-SR were increased in the first week of DBI ,then the threshold continuously increased in the second and third week , at last the threshold decreased in the fourth week .The results under scaning electron microscope demonstrated that the ciliums of the majority of outer hair cells lodged in the first week of DBI .The results under transmission electron microscope showed that in the first week of DBI ,there were edema and denuration of mitochondrial ,mitochondrial cristaes were obscured or disappeared .The changes were deteriorate in the second and third week ,whereas the changes were mitigatal in the fourth week .Conclusion Cochlear morphology and hearing damage were observed in diffuse brain injury model of rats .

Objective To explore the effects of Zinc Protoporphyrin and Heme on the expression of HO-1 in cochlear and the change of auditory brainstem response on diffuse traumatic brain injury model of rats.Methods Diffuse traumatic brain injury model of rats were established and randomly divided into thirteen groups.Then auditory brainstem response examination,light microscope,immunohistochemistry technique were used to evaluate the change of auditory brainstem response and the expression of HO-1 in cochlear.Results The differences of auditory brainstem response threshold and latency of wave between the experimental and the normal control group were obvious(P＜0.05).The expression of HO-1 in the control group was normal,whereas there were obvious changes of inner ear HO-1 expression in the traumatic groups.The grey value of HO-1 expression in trauma group,Znpp group and heme group was significantly associated with auditory function change(P＜0.05).Conclusion There were influence of Zinc Protoporphyrin and Hemeon the inner ear HO-1 expression and the change of auditory brainstem response with diffuse traumatic brain injury model of rats.The protective effect of heme on auditory function may be associated with the increased expression of HO-1.

Objective:To analyze results of microbiological culture of lesion exudates and drug sensitivity test in patients with pemphigus, and to optimize the management protocol of pemphigus.Methods:Inpatients with pemphigus were collected from Department of Dermatology and Venereology, the First Affiliated Hospital of Anhui Medical University from January to December in 2008, and the results of microbiological culture of skin lesion exudates and drug sensitivity test were retrospectively analyzed.Results:A total of 176 patients were collected, and skin lesion exudate culture yielded 25 kinds of bacteria and 11 kinds of fungi in 156 (88.64%) patients. Of the 156 patients, 1 kind of pathogen was yielded in 104 (66.67%), and 2 or more kinds of pathogens were yielded in 52 (33.33%). Gram-positive cocci were detected in 134 (76.14%) cases, including Staphylococcus aureus in 80, Staphylococcus hemolyticus in 21, and Staphylococcus epidermidis in 16; Gram-negative bacilli were detected in 66 (37.50%) cases; fungi were detected in 24 (13.64%) cases. In addition, 89 (93.68%) of 95 relapsed patients showed positive culture results, 67 (82.72%) of 81 patients with newly developed pemphigus had positive culture results, and the positive rate significantly differed between the two groups ( χ2 = 5.22, P <0.05). Drug sensitivity test for Staphylococcus aureus isolates showed that 71 (97.26%) of 73 were sensitive to vancomycin, 69 (97.18%) of 71 to linezolid, and 61 (87.14%) of 70 to rifampin. Conclusion:Cutaneous infection, especially Gram-positive cocci infection, is very common in patients with pemphigus, and Staphylococcus aureus clinical isolates were highly sensitive to vancomycin, linezolid and rifampicin.

OBJECTIVETo explore the possible genetic model of psoriasis vulgaris.

METHODSThe complex segregation analysis and heritability calculation were performed with the aid of Penrose method, Falconer regression method and SAGE-REGTL program.

RESULTSIt was found that in 1043 patients with psoriasis vulgaris, 305 patients (29.24%) have the family history of psoriasis, and 738 patients have not the family history. A ratio of s/q approached 1/(square root of q) with Penrose method, and the heritability values of psoriasis in the first-degree and second-degree relatives were 67.04%, 46.6% respectively. By complex segregation analysis, Mendelian, non-major-gene model and environment model were rejected for psoriasis.

CONCLUSIONThe results suggest that psoriasis follows a pattern of polygenetic or multifactorial inheritance rather than single-gene inheritance.

Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Data Interpretation, Statistical ; Family Health ; Female ; Humans ; Male ; Middle Aged ; Models, Genetic ; Psoriasis ; genetics