ObjectiveTo investigate the mechanism by which tetracycline inhibits the development of experimental abdominal aortic aneurysms (AAA).MethodsMale Wistar rats underwent intraluminal perfusion of the abdominal aorta with 50 units of porcine pancreatic elastase followed by treatment for 14 days with tetracycline (25?mg/day, subcutaneously; n=10) or with NS alone (n=10). Aortic wall elastin and collagen concentrations were measured with image analysis system and fixed aortic tissues were examined by light microscopy for inflammation. The expression of MMP-2 and MMP-9 were observed in aortic tissues using immunohistochemistry and molecular in situ histohybridization technique. Results AAAs developed in all NS-treated rats, and only 1/10 of tetracycline-treated rats at 14 days(P

Objective:To construct a canine thoracic aortic dissection(TAD) model for later studying. Methods:The soft tube with graduation and the puncture needle were used to produce the appliance for making TAD model.Under X ray, the appliance was placed into the canine thoracic aortic for elastin enzyme perfusion through iliac arteries or abdominal aorta. Results:TAD were showed instantly,2 weeks and 1 month later by angiography. Conclusion:This appliance can construct a canine TAD model.

0.05). The CYFRA21-1 level measured at the fifth day after treatment in death group was higher than that in survival group (P

Objective To evaluate the surgical treatment of carotid artery stenosis. MethodAccording to the level and degree of the stenosis, different operations were performed on 120 patients who suffered from the extracranial carotid stenosis. Treatment result was retrospectively reviewed. ResultSurgery was successful in all the patients. We performed carotid endarterectomy on 111 cases and other operations on 9 cases. The incidence of postoperative complications was low, especially for carotid endarterectomy. ConclusionCarotid endarterectomy is still the main therapy for the treatment of carotid artery stenosis with a satisfactory result.

Objective To investigate the relationship between the polymorphism of cytochrome P450 19 (CYP19) gene rs10046 and the risk of colon and rectal cancer.Methods Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to analyze gene polymorphism in CYP 19 gene rs10046 in 198 cases of colon and rectal cancer patients (case group) and 309 cases of healthy controls (control group).The genotype frequency and relative risk of CYP19 gene rs10046 between the two groups were compared and the relationship with the clinicopathological features of colorectal cancer was analyzed.Results In case group,the prevalence rates of CYP19 rs10046 genotypes C/C,C/T and T/T were 28.3％,44.4％ and 27.3％,respectively,and 17.2％,51.8％ and 31.1％ in the control group,respectively,with statistical significant difference (P ＜ 0.05).Compared with wild-type C/C,the susceptibility of colorectal cancer with the genotypes of C/T and T/T was decreased by 0.521 (95％ CI:0.330-0.822)and 0.532 (95 ％ CI:0.322-0.880) respectively.Moveover,in the non-smoking group,the risk of colorectal cancer with genotype T/T or C/T was decreased by 0.409 (95％ CI:0.210-0.798) compared with genotype C/C.The interaction was not exist in smoking group.Conclusions The polymorphism of CYP19 gene rs10046 is related to the susceptibility of colon and rectal cancer.The T/T,C/T genotype of CYP19 rs10046 decrease the risk of colon and rectal cancer,and which might be the protective factor of colon and rectal cancer.